Effects of limonin on oxidative stress and early apoptosis in oocytes during in vitro maturation
To investigate the effects of limonin (Lim) on oxidative stress and early apoptosis in bovine oocytes during in vitro maturation (IVM), different concentrations of Lim (0, 10, 20, 50 μmol/L) were added to bovine IVM medium. Oocyte maturation rates and development 24 h after in vitro fertilization (I...
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| Veröffentlicht in: | Theriogenology 2024-04, Vol.218, p.8-15 |
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| creator | Jiao, Anhui Sun, Jingyu Sun, Zhaoyang Zhao, Yuhan Han, Tiancang Zhang, Hongbo Gao, Qingshan |
| description | To investigate the effects of limonin (Lim) on oxidative stress and early apoptosis in bovine oocytes during in vitro maturation (IVM), different concentrations of Lim (0, 10, 20, 50 μmol/L) were added to bovine IVM medium. Oocyte maturation rates and development 24 h after in vitro fertilization (IVF) were examined to determine the optimal Lim concentration. The optimal Lim concentration was added to the IVM medium, and 0 μmol/L Lim was used as the control. Immunofluorescence staining was used to detect the abnormal rate of spindle assembly, reactive oxygen species (ROS), glutathione (GSH), mitochondrial membrane potential (MMP) levels, mitochondrial distribution, and the fluorescence intensity of cathepsin B (CB)-active LC3 protein. RT‒qPCR was used to detect the mRNA expression levels of antioxidant-, apoptosis- and autophagy-related genes in oocytes. The total number of blastocysts and the proportion of apoptotic cells among blastocysts were detected. The results showed that the PBI ejection rate, cleavage rate and blastocyst rate of bovine oocytes in the 20 μmol/L Lim group were significantly higher than those in the control group (P |
| doi_str_mv | 10.1016/j.theriogenology.2024.01.025 |
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•Limonin is a natural antioxidant.•Its role in the repair of oxidative damage has attracted more and more attention from researchers, but the specific role of limonin in animal reproduction, especially in female gamete development, is rarely studied.•Lim has a positive effect on the IVM of bovine oocytes by regulating REDOX homeostasis during IVM to reduce spindle damage and enhance mitochondrial function, thereby inhibiting oocyte apoptosis and autophagy.]]></description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2024.01.025</identifier><identifier>PMID: 38290232</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Apoptosis ; Bovine oocytes ; Development of embryos ; IVM ; Limonin</subject><ispartof>Theriogenology, 2024-04, Vol.218, p.8-15</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c327t-43085700b8c178118b00bfea75dcaff3814f102ba7205faaa6c654e948042f193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2024.01.025$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38290232$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiao, Anhui</creatorcontrib><creatorcontrib>Sun, Jingyu</creatorcontrib><creatorcontrib>Sun, Zhaoyang</creatorcontrib><creatorcontrib>Zhao, Yuhan</creatorcontrib><creatorcontrib>Han, Tiancang</creatorcontrib><creatorcontrib>Zhang, Hongbo</creatorcontrib><creatorcontrib>Gao, Qingshan</creatorcontrib><title>Effects of limonin on oxidative stress and early apoptosis in oocytes during in vitro maturation</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description><![CDATA[To investigate the effects of limonin (Lim) on oxidative stress and early apoptosis in bovine oocytes during in vitro maturation (IVM), different concentrations of Lim (0, 10, 20, 50 μmol/L) were added to bovine IVM medium. Oocyte maturation rates and development 24 h after in vitro fertilization (IVF) were examined to determine the optimal Lim concentration. The optimal Lim concentration was added to the IVM medium, and 0 μmol/L Lim was used as the control. Immunofluorescence staining was used to detect the abnormal rate of spindle assembly, reactive oxygen species (ROS), glutathione (GSH), mitochondrial membrane potential (MMP) levels, mitochondrial distribution, and the fluorescence intensity of cathepsin B (CB)-active LC3 protein. RT‒qPCR was used to detect the mRNA expression levels of antioxidant-, apoptosis- and autophagy-related genes in oocytes. The total number of blastocysts and the proportion of apoptotic cells among blastocysts were detected. The results showed that the PBI ejection rate, cleavage rate and blastocyst rate of bovine oocytes in the 20 μmol/L Lim group were significantly higher than those in the control group (P < 0.05). Compared with those in the control group, ROS levels, abnormal mitochondrial distribution, the proportion of abnormal spindle assembly, CB activity and LC3 protein fluorescence intensity of oocytes in the 20 μmol/L Lim group were significantly decreased (P < 0.05), and GSH and MMP levels were significantly increased (P < 0.05). The expression of antioxidant genes (Prdx3, Prdx6, Sirt1) and antiapoptotic genes (Bcl-xl, Survivin) were significantly upregulated (P < 0.05), and the expression levels of proapoptotic genes (Caspase-4, BAX) and autophagy-related genes (LC3) were significantly downregulated (P < 0.05). The total number of cells among in vitro fertilized embryos was significantly increased (P < 0.05), and the apoptosis rate of blastocysts was significantly decreased (P < 0.05). Here, we show that Lim exerts positive effects on bovine oocyte IVM by regulating REDOX homeostasis, reducing spindle damage and enhancing mitochondrial function during IVM, thereby inhibiting oocyte apoptosis and autophagy.
•Limonin is a natural antioxidant.•Its role in the repair of oxidative damage has attracted more and more attention from researchers, but the specific role of limonin in animal reproduction, especially in female gamete development, is rarely studied.•Lim has a positive effect on the IVM of bovine oocytes by regulating REDOX homeostasis during IVM to reduce spindle damage and enhance mitochondrial function, thereby inhibiting oocyte apoptosis and autophagy.]]></description><subject>Apoptosis</subject><subject>Bovine oocytes</subject><subject>Development of embryos</subject><subject>IVM</subject><subject>Limonin</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkE1rGzEQhkVJady0f6Ho0EMuu5mR9hN6CSFpA4FcWuhNlbUjV2Z35UpaE__7yjgt5BYQSIjnfYd5GPuMUCJgc7Ut028Kzm9o9qPfHEoBoioBSxD1G7bCru0LKSSesRVAL4umx5_n7H2MWwCQTYPv2LnsRA9CihX7dWstmRS5t3x0k5_dzH0-T27Qye2JxxQoRq7ngZMO44Hrnd8lH13kR9SbQ6LIhyW4eXP82bsUPJ90WkIu8PMH9tbqMdLH5_uC_bi7_X7zrXh4_Hp_c_1QGCnaVFQSuroFWHcG2w6xW-e3Jd3Wg9HWyg4riyDWuhVQW611Y5q6or7qoBIWe3nBLk-9u-D_LBSTmlw0NI56Jr9EJfocbHMFZvTLCTXBxxjIql1wkw4HhaCOjtVWvXSsjo4VoMqOc_zT86RlPdHwP_xPagbuTgDlffeOgorG0WxocCG7VoN3r5v0F_gUl7s</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Jiao, Anhui</creator><creator>Sun, Jingyu</creator><creator>Sun, Zhaoyang</creator><creator>Zhao, Yuhan</creator><creator>Han, Tiancang</creator><creator>Zhang, Hongbo</creator><creator>Gao, Qingshan</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240401</creationdate><title>Effects of limonin on oxidative stress and early apoptosis in oocytes during in vitro maturation</title><author>Jiao, Anhui ; Sun, Jingyu ; Sun, Zhaoyang ; Zhao, Yuhan ; Han, Tiancang ; Zhang, Hongbo ; Gao, Qingshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-43085700b8c178118b00bfea75dcaff3814f102ba7205faaa6c654e948042f193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Apoptosis</topic><topic>Bovine oocytes</topic><topic>Development of embryos</topic><topic>IVM</topic><topic>Limonin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiao, Anhui</creatorcontrib><creatorcontrib>Sun, Jingyu</creatorcontrib><creatorcontrib>Sun, Zhaoyang</creatorcontrib><creatorcontrib>Zhao, Yuhan</creatorcontrib><creatorcontrib>Han, Tiancang</creatorcontrib><creatorcontrib>Zhang, Hongbo</creatorcontrib><creatorcontrib>Gao, Qingshan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiao, Anhui</au><au>Sun, Jingyu</au><au>Sun, Zhaoyang</au><au>Zhao, Yuhan</au><au>Han, Tiancang</au><au>Zhang, Hongbo</au><au>Gao, Qingshan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of limonin on oxidative stress and early apoptosis in oocytes during in vitro maturation</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>218</volume><spage>8</spage><epage>15</epage><pages>8-15</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract><![CDATA[To investigate the effects of limonin (Lim) on oxidative stress and early apoptosis in bovine oocytes during in vitro maturation (IVM), different concentrations of Lim (0, 10, 20, 50 μmol/L) were added to bovine IVM medium. Oocyte maturation rates and development 24 h after in vitro fertilization (IVF) were examined to determine the optimal Lim concentration. The optimal Lim concentration was added to the IVM medium, and 0 μmol/L Lim was used as the control. Immunofluorescence staining was used to detect the abnormal rate of spindle assembly, reactive oxygen species (ROS), glutathione (GSH), mitochondrial membrane potential (MMP) levels, mitochondrial distribution, and the fluorescence intensity of cathepsin B (CB)-active LC3 protein. RT‒qPCR was used to detect the mRNA expression levels of antioxidant-, apoptosis- and autophagy-related genes in oocytes. The total number of blastocysts and the proportion of apoptotic cells among blastocysts were detected. The results showed that the PBI ejection rate, cleavage rate and blastocyst rate of bovine oocytes in the 20 μmol/L Lim group were significantly higher than those in the control group (P < 0.05). Compared with those in the control group, ROS levels, abnormal mitochondrial distribution, the proportion of abnormal spindle assembly, CB activity and LC3 protein fluorescence intensity of oocytes in the 20 μmol/L Lim group were significantly decreased (P < 0.05), and GSH and MMP levels were significantly increased (P < 0.05). The expression of antioxidant genes (Prdx3, Prdx6, Sirt1) and antiapoptotic genes (Bcl-xl, Survivin) were significantly upregulated (P < 0.05), and the expression levels of proapoptotic genes (Caspase-4, BAX) and autophagy-related genes (LC3) were significantly downregulated (P < 0.05). The total number of cells among in vitro fertilized embryos was significantly increased (P < 0.05), and the apoptosis rate of blastocysts was significantly decreased (P < 0.05). Here, we show that Lim exerts positive effects on bovine oocyte IVM by regulating REDOX homeostasis, reducing spindle damage and enhancing mitochondrial function during IVM, thereby inhibiting oocyte apoptosis and autophagy.
•Limonin is a natural antioxidant.•Its role in the repair of oxidative damage has attracted more and more attention from researchers, but the specific role of limonin in animal reproduction, especially in female gamete development, is rarely studied.•Lim has a positive effect on the IVM of bovine oocytes by regulating REDOX homeostasis during IVM to reduce spindle damage and enhance mitochondrial function, thereby inhibiting oocyte apoptosis and autophagy.]]></abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38290232</pmid><doi>10.1016/j.theriogenology.2024.01.025</doi><tpages>8</tpages></addata></record> |
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| subjects | Apoptosis Bovine oocytes Development of embryos IVM Limonin |
| title | Effects of limonin on oxidative stress and early apoptosis in oocytes during in vitro maturation |
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