Association of the PDE4D gene variant with selected markers in individuals with ischaemic heart disease: a pilot study
The aim of the study was to evaluate the variant (rs2910829) of the PDE4D gene in relation to its influence on biochemical, anthropometric and physiological parameters in patients with coronary artery disease and healthy subjects of the Eastern Slovak population. The male group consisted of 72 indiv...
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| Veröffentlicht in: | Central European journal of public health 2023-12, Vol.31 (Suppl 1), p.S75-S81 |
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| creator | Mydlárová Blaščáková, Marta Hricová, Katarína Mašlejová, Katarína Majherová, Mária Vašková, Hedviga Poráčová, Janka Bernátová, Renáta Konečná, Mária Sedlák, Vincent |
| description | The aim of the study was to evaluate the variant (rs2910829) of the PDE4D gene in relation to its influence on biochemical, anthropometric and physiological parameters in patients with coronary artery disease and healthy subjects of the Eastern Slovak population.
The male group consisted of 72 individuals and the female group consisted of 132 individuals. On the basis of clinical screening the subjects were divided into two groups - with ischaemic heart disease and control group. Genomic DNA was isolated from peripheral blood using a commercial NucleoSpin
Blood Machenery-Nagel kit. Molecular genetic analysis of the polymorphism under study was performed using the StepOne™ Real-Time PCR System instrument. The lipid profile markers TC, HDL, LDL, TG were measured by Cobas Integra 400 plus biochemical analyser, and systolic and diastolic blood pressure using a digital blood pressure monitor. Among anthropometric parameters, body height and weight, waist and hip circumference were measured and BMI and WHR indices were calculated.
A statistically significant (p = 0.018) possible association between the mutant T allele and ischaemic heart disease was found in men. In women, we found a statistically significant difference in the systolic (p = 0.013) and diastolic blood parameters (p = 0.005) in the CC genotype. In the group of women, we found statistically significant differences in all observed anthropometric parameters and in LDL and TC markers. In the group of men divided on the basis of BMI, statistical significance was found in systolic blood pressure (p = 0.028). In the group of women with ischaemic heart disease, we found a negative correlation between BMI and HDL.
The study contributes to new findings of the representation of genotypes and alleles of the rs2910829 PDE4D gene polymorphism in the Slovak population. This is a pilot study. Interactions between genotype and observed anthropometric, physiological and biochemical markers were confirmed. |
| doi_str_mv | 10.21101/cejph.a7843 |
| format | Article |
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The male group consisted of 72 individuals and the female group consisted of 132 individuals. On the basis of clinical screening the subjects were divided into two groups - with ischaemic heart disease and control group. Genomic DNA was isolated from peripheral blood using a commercial NucleoSpin
Blood Machenery-Nagel kit. Molecular genetic analysis of the polymorphism under study was performed using the StepOne™ Real-Time PCR System instrument. The lipid profile markers TC, HDL, LDL, TG were measured by Cobas Integra 400 plus biochemical analyser, and systolic and diastolic blood pressure using a digital blood pressure monitor. Among anthropometric parameters, body height and weight, waist and hip circumference were measured and BMI and WHR indices were calculated.
A statistically significant (p = 0.018) possible association between the mutant T allele and ischaemic heart disease was found in men. In women, we found a statistically significant difference in the systolic (p = 0.013) and diastolic blood parameters (p = 0.005) in the CC genotype. In the group of women, we found statistically significant differences in all observed anthropometric parameters and in LDL and TC markers. In the group of men divided on the basis of BMI, statistical significance was found in systolic blood pressure (p = 0.028). In the group of women with ischaemic heart disease, we found a negative correlation between BMI and HDL.
The study contributes to new findings of the representation of genotypes and alleles of the rs2910829 PDE4D gene polymorphism in the Slovak population. This is a pilot study. Interactions between genotype and observed anthropometric, physiological and biochemical markers were confirmed.</description><identifier>ISSN: 1210-7778</identifier><identifier>EISSN: 1803-1048</identifier><identifier>DOI: 10.21101/cejph.a7843</identifier><identifier>PMID: 38272484</identifier><language>eng</language><publisher>Czech Republic: National Institute of Public Health</publisher><subject>Alleles ; Anthropometry ; Atherosclerosis ; Biochemical markers ; Biochemistry ; Blood pressure ; Body height ; Body mass index ; Cardiovascular disease ; Cardiovascular diseases ; Coronary artery disease ; Coronary vessels ; Disease control ; Gene polymorphism ; Genetic analysis ; Genotype & phenotype ; Genotypes ; Health care ; Heart ; Heart diseases ; High density lipoprotein ; Ischemia ; Lipids ; Low density lipoprotein ; Men ; Mortality ; Parameters ; Peripheral blood ; Phosphodiesterase ; Physiology ; Pilot projects ; Polymorphism ; Population ; Population genetics ; Statistical analysis ; Vein & artery diseases ; Women ; Womens health</subject><ispartof>Central European journal of public health, 2023-12, Vol.31 (Suppl 1), p.S75-S81</ispartof><rights>Copyright National Institute of Public Health Dec 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c314t-9730ad2d56075c9d3af76b30915fba925ce727bb7784aeae8546ab6c7de8a393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38272484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mydlárová Blaščáková, Marta</creatorcontrib><creatorcontrib>Hricová, Katarína</creatorcontrib><creatorcontrib>Mašlejová, Katarína</creatorcontrib><creatorcontrib>Majherová, Mária</creatorcontrib><creatorcontrib>Vašková, Hedviga</creatorcontrib><creatorcontrib>Poráčová, Janka</creatorcontrib><creatorcontrib>Bernátová, Renáta</creatorcontrib><creatorcontrib>Konečná, Mária</creatorcontrib><creatorcontrib>Sedlák, Vincent</creatorcontrib><title>Association of the PDE4D gene variant with selected markers in individuals with ischaemic heart disease: a pilot study</title><title>Central European journal of public health</title><addtitle>Cent Eur J Public Health</addtitle><description>The aim of the study was to evaluate the variant (rs2910829) of the PDE4D gene in relation to its influence on biochemical, anthropometric and physiological parameters in patients with coronary artery disease and healthy subjects of the Eastern Slovak population.
The male group consisted of 72 individuals and the female group consisted of 132 individuals. On the basis of clinical screening the subjects were divided into two groups - with ischaemic heart disease and control group. Genomic DNA was isolated from peripheral blood using a commercial NucleoSpin
Blood Machenery-Nagel kit. Molecular genetic analysis of the polymorphism under study was performed using the StepOne™ Real-Time PCR System instrument. The lipid profile markers TC, HDL, LDL, TG were measured by Cobas Integra 400 plus biochemical analyser, and systolic and diastolic blood pressure using a digital blood pressure monitor. Among anthropometric parameters, body height and weight, waist and hip circumference were measured and BMI and WHR indices were calculated.
A statistically significant (p = 0.018) possible association between the mutant T allele and ischaemic heart disease was found in men. In women, we found a statistically significant difference in the systolic (p = 0.013) and diastolic blood parameters (p = 0.005) in the CC genotype. In the group of women, we found statistically significant differences in all observed anthropometric parameters and in LDL and TC markers. In the group of men divided on the basis of BMI, statistical significance was found in systolic blood pressure (p = 0.028). In the group of women with ischaemic heart disease, we found a negative correlation between BMI and HDL.
The study contributes to new findings of the representation of genotypes and alleles of the rs2910829 PDE4D gene polymorphism in the Slovak population. This is a pilot study. Interactions between genotype and observed anthropometric, physiological and biochemical markers were confirmed.</description><subject>Alleles</subject><subject>Anthropometry</subject><subject>Atherosclerosis</subject><subject>Biochemical markers</subject><subject>Biochemistry</subject><subject>Blood pressure</subject><subject>Body height</subject><subject>Body mass index</subject><subject>Cardiovascular disease</subject><subject>Cardiovascular diseases</subject><subject>Coronary artery disease</subject><subject>Coronary vessels</subject><subject>Disease control</subject><subject>Gene polymorphism</subject><subject>Genetic analysis</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Health care</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>High density lipoprotein</subject><subject>Ischemia</subject><subject>Lipids</subject><subject>Low density lipoprotein</subject><subject>Men</subject><subject>Mortality</subject><subject>Parameters</subject><subject>Peripheral blood</subject><subject>Phosphodiesterase</subject><subject>Physiology</subject><subject>Pilot projects</subject><subject>Polymorphism</subject><subject>Population</subject><subject>Population genetics</subject><subject>Statistical analysis</subject><subject>Vein & artery diseases</subject><subject>Women</subject><subject>Womens 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lipoprotein</topic><topic>Ischemia</topic><topic>Lipids</topic><topic>Low density lipoprotein</topic><topic>Men</topic><topic>Mortality</topic><topic>Parameters</topic><topic>Peripheral blood</topic><topic>Phosphodiesterase</topic><topic>Physiology</topic><topic>Pilot projects</topic><topic>Polymorphism</topic><topic>Population</topic><topic>Population genetics</topic><topic>Statistical analysis</topic><topic>Vein & artery diseases</topic><topic>Women</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mydlárová Blaščáková, Marta</creatorcontrib><creatorcontrib>Hricová, Katarína</creatorcontrib><creatorcontrib>Mašlejová, Katarína</creatorcontrib><creatorcontrib>Majherová, Mária</creatorcontrib><creatorcontrib>Vašková, Hedviga</creatorcontrib><creatorcontrib>Poráčová, Janka</creatorcontrib><creatorcontrib>Bernátová, Renáta</creatorcontrib><creatorcontrib>Konečná, Mária</creatorcontrib><creatorcontrib>Sedlák, 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Academic</collection><jtitle>Central European journal of public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mydlárová Blaščáková, Marta</au><au>Hricová, Katarína</au><au>Mašlejová, Katarína</au><au>Majherová, Mária</au><au>Vašková, Hedviga</au><au>Poráčová, Janka</au><au>Bernátová, Renáta</au><au>Konečná, Mária</au><au>Sedlák, Vincent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of the PDE4D gene variant with selected markers in individuals with ischaemic heart disease: a pilot study</atitle><jtitle>Central European journal of public health</jtitle><addtitle>Cent Eur J Public Health</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>31</volume><issue>Suppl 1</issue><spage>S75</spage><epage>S81</epage><pages>S75-S81</pages><issn>1210-7778</issn><eissn>1803-1048</eissn><abstract>The aim of the study was to evaluate the variant (rs2910829) of the PDE4D gene in relation to its influence on biochemical, anthropometric and physiological parameters in patients with coronary artery disease and healthy subjects of the Eastern Slovak population.
The male group consisted of 72 individuals and the female group consisted of 132 individuals. On the basis of clinical screening the subjects were divided into two groups - with ischaemic heart disease and control group. Genomic DNA was isolated from peripheral blood using a commercial NucleoSpin
Blood Machenery-Nagel kit. Molecular genetic analysis of the polymorphism under study was performed using the StepOne™ Real-Time PCR System instrument. The lipid profile markers TC, HDL, LDL, TG were measured by Cobas Integra 400 plus biochemical analyser, and systolic and diastolic blood pressure using a digital blood pressure monitor. Among anthropometric parameters, body height and weight, waist and hip circumference were measured and BMI and WHR indices were calculated.
A statistically significant (p = 0.018) possible association between the mutant T allele and ischaemic heart disease was found in men. In women, we found a statistically significant difference in the systolic (p = 0.013) and diastolic blood parameters (p = 0.005) in the CC genotype. In the group of women, we found statistically significant differences in all observed anthropometric parameters and in LDL and TC markers. In the group of men divided on the basis of BMI, statistical significance was found in systolic blood pressure (p = 0.028). In the group of women with ischaemic heart disease, we found a negative correlation between BMI and HDL.
The study contributes to new findings of the representation of genotypes and alleles of the rs2910829 PDE4D gene polymorphism in the Slovak population. This is a pilot study. Interactions between genotype and observed anthropometric, physiological and biochemical markers were confirmed.</abstract><cop>Czech Republic</cop><pub>National Institute of Public Health</pub><pmid>38272484</pmid><doi>10.21101/cejph.a7843</doi><oa>free_for_read</oa></addata></record> |
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| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Alleles Anthropometry Atherosclerosis Biochemical markers Biochemistry Blood pressure Body height Body mass index Cardiovascular disease Cardiovascular diseases Coronary artery disease Coronary vessels Disease control Gene polymorphism Genetic analysis Genotype & phenotype Genotypes Health care Heart Heart diseases High density lipoprotein Ischemia Lipids Low density lipoprotein Men Mortality Parameters Peripheral blood Phosphodiesterase Physiology Pilot projects Polymorphism Population Population genetics Statistical analysis Vein & artery diseases Women Womens health |
| title | Association of the PDE4D gene variant with selected markers in individuals with ischaemic heart disease: a pilot study |
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