Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand
Hookworm infections remain a significant public health concern in tropical and subtropical regions, including Thailand. This study investigated the species and genetic diversity of hookworm infections in domestic dogs from northeastern Thailand. The molecular analysis focused on amplifying and seque...
Gespeichert in:
| Veröffentlicht in: | Parasitology research (1987) 2024-02, Vol.123 (2), p.115-115, Article 115 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 115 |
|---|---|
| container_issue | 2 |
| container_start_page | 115 |
| container_title | Parasitology research (1987) |
| container_volume | 123 |
| creator | Rodpai, Rutchanee Sanpool, Oranuch Sadaow, Lakkhana Boonroumkaew, Patcharaporn Intapan, Pewpan M. Maleewong, Wanchai Yingklang, Manachai Janwan, Penchom Vaisusuk, Kotchaphon Chatan, Wasupon Piratae, Supawadee Thanchomnang, Tongjit |
| description | Hookworm infections remain a significant public health concern in tropical and subtropical regions, including Thailand. This study investigated the species and genetic diversity of hookworm infections in domestic dogs from northeastern Thailand. The molecular analysis focused on amplifying and sequencing specific regions of ribosomal RNA genes (ITS1-5.8S-ITS2 region) and the mitochondrial cytochrome c oxidase subunit 1 (
cox
1) gene in hookworm larvae recovered from 21 domestic dog stool samples. Among 21 larvae (one larva per infected dog) analyzed, 14 had sequences identical to
Ancylostoma caninum
, and 7 showed sequences almost identical to
Ancylostoma ceylanicum
. Phylogenetic analysis of
cox
1 sequences placed
A. caninum
and
A. ceylanicum
in separate clades. The median-joining network of
A. caninum cox
1 sequences from Thailand showed high haplotype diversity and belonged to the same cluster as sequences from Australia while forming separate clusters from those of
A. caninum
samples from the USA. The available published
A. ceylanicum cox
1 sequences (
n
= 33), in combination with seven sequences in the present study, represented 15 haplotypes distributed among three clusters. Interestingly,
A. ceylanicum
sequences from dogs and humans shared the same haplotypes. These findings are crucial for recognizing the potential for zoonotic transmission, highlighting the necessity for targeted control measures, and increasing awareness among pet owners and healthcare professionals to mitigate the risk of hookworm transmission to humans. |
| doi_str_mv | 10.1007/s00436-024-08134-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2919744912</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2919803495</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-bb60e77de4a516671344ab16b94577f978ac782c3d88b427471f6db33175745b3</originalsourceid><addsrcrecordid>eNp9kUtv1DAUhS0EokPhD7BAltiwIOBnHC9RxUsqYlPWluPczLgkvsVOQB3-PJ6ZAhILVr6yv3PusQ4hTzl7xRkzrwtjSrYNE6phHZeq2d8jG66kaLjV-j7ZMFtnxrk8I49KuWaMm1aph-RMdqLTXNgN-fkJJwjr5DONA6QljjH4JWKiPg10CwmWGOgQv0MucbmlONI9YsLD7Q7x6w_MM41phHAQlTrSAWcoRxVuCx0zzjRhXnbgywI5vaRXOx-nav-YPBj9VODJ3XlOvrx7e3Xxobn8_P7jxZvLJkijl6bvWwbGDKC85m1r6k-V73nbW6WNGa3pfDCdCHLoul4Jowwf26GXkhttlO7lOXlx8r3J-G2t2dwcS4CpZgBcixOWW6OU5aKiz_9Br3HNqaY7Uh2TyupKiRMVMpaSYXQ3Oc4-3zrO3KEad6rG1WrcsRq3r6Jnd9ZrP8PwR_K7iwrIE1DqU9pC_rv7P7a_ANKnm2E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2919803495</pqid></control><display><type>article</type><title>Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand</title><source>SpringerLink Journals</source><creator>Rodpai, Rutchanee ; Sanpool, Oranuch ; Sadaow, Lakkhana ; Boonroumkaew, Patcharaporn ; Intapan, Pewpan M. ; Maleewong, Wanchai ; Yingklang, Manachai ; Janwan, Penchom ; Vaisusuk, Kotchaphon ; Chatan, Wasupon ; Piratae, Supawadee ; Thanchomnang, Tongjit</creator><creatorcontrib>Rodpai, Rutchanee ; Sanpool, Oranuch ; Sadaow, Lakkhana ; Boonroumkaew, Patcharaporn ; Intapan, Pewpan M. ; Maleewong, Wanchai ; Yingklang, Manachai ; Janwan, Penchom ; Vaisusuk, Kotchaphon ; Chatan, Wasupon ; Piratae, Supawadee ; Thanchomnang, Tongjit</creatorcontrib><description>Hookworm infections remain a significant public health concern in tropical and subtropical regions, including Thailand. This study investigated the species and genetic diversity of hookworm infections in domestic dogs from northeastern Thailand. The molecular analysis focused on amplifying and sequencing specific regions of ribosomal RNA genes (ITS1-5.8S-ITS2 region) and the mitochondrial cytochrome c oxidase subunit 1 (
cox
1) gene in hookworm larvae recovered from 21 domestic dog stool samples. Among 21 larvae (one larva per infected dog) analyzed, 14 had sequences identical to
Ancylostoma caninum
, and 7 showed sequences almost identical to
Ancylostoma ceylanicum
. Phylogenetic analysis of
cox
1 sequences placed
A. caninum
and
A. ceylanicum
in separate clades. The median-joining network of
A. caninum cox
1 sequences from Thailand showed high haplotype diversity and belonged to the same cluster as sequences from Australia while forming separate clusters from those of
A. caninum
samples from the USA. The available published
A. ceylanicum cox
1 sequences (
n
= 33), in combination with seven sequences in the present study, represented 15 haplotypes distributed among three clusters. Interestingly,
A. ceylanicum
sequences from dogs and humans shared the same haplotypes. These findings are crucial for recognizing the potential for zoonotic transmission, highlighting the necessity for targeted control measures, and increasing awareness among pet owners and healthcare professionals to mitigate the risk of hookworm transmission to humans.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-024-08134-z</identifier><identifier>PMID: 38285129</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cytochrome-c oxidase ; Dogs ; Domestic animals ; Genetic diversity ; Haplotypes ; Immunology ; Infections ; Larvae ; Medical Microbiology ; Microbiology ; Parasites ; Phylogeny ; Public health ; rRNA ; rRNA 5.8S ; Zoonoses</subject><ispartof>Parasitology research (1987), 2024-02, Vol.123 (2), p.115-115, Article 115</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-bb60e77de4a516671344ab16b94577f978ac782c3d88b427471f6db33175745b3</citedby><cites>FETCH-LOGICAL-c375t-bb60e77de4a516671344ab16b94577f978ac782c3d88b427471f6db33175745b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-024-08134-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-024-08134-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38285129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodpai, Rutchanee</creatorcontrib><creatorcontrib>Sanpool, Oranuch</creatorcontrib><creatorcontrib>Sadaow, Lakkhana</creatorcontrib><creatorcontrib>Boonroumkaew, Patcharaporn</creatorcontrib><creatorcontrib>Intapan, Pewpan M.</creatorcontrib><creatorcontrib>Maleewong, Wanchai</creatorcontrib><creatorcontrib>Yingklang, Manachai</creatorcontrib><creatorcontrib>Janwan, Penchom</creatorcontrib><creatorcontrib>Vaisusuk, Kotchaphon</creatorcontrib><creatorcontrib>Chatan, Wasupon</creatorcontrib><creatorcontrib>Piratae, Supawadee</creatorcontrib><creatorcontrib>Thanchomnang, Tongjit</creatorcontrib><title>Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Hookworm infections remain a significant public health concern in tropical and subtropical regions, including Thailand. This study investigated the species and genetic diversity of hookworm infections in domestic dogs from northeastern Thailand. The molecular analysis focused on amplifying and sequencing specific regions of ribosomal RNA genes (ITS1-5.8S-ITS2 region) and the mitochondrial cytochrome c oxidase subunit 1 (
cox
1) gene in hookworm larvae recovered from 21 domestic dog stool samples. Among 21 larvae (one larva per infected dog) analyzed, 14 had sequences identical to
Ancylostoma caninum
, and 7 showed sequences almost identical to
Ancylostoma ceylanicum
. Phylogenetic analysis of
cox
1 sequences placed
A. caninum
and
A. ceylanicum
in separate clades. The median-joining network of
A. caninum cox
1 sequences from Thailand showed high haplotype diversity and belonged to the same cluster as sequences from Australia while forming separate clusters from those of
A. caninum
samples from the USA. The available published
A. ceylanicum cox
1 sequences (
n
= 33), in combination with seven sequences in the present study, represented 15 haplotypes distributed among three clusters. Interestingly,
A. ceylanicum
sequences from dogs and humans shared the same haplotypes. These findings are crucial for recognizing the potential for zoonotic transmission, highlighting the necessity for targeted control measures, and increasing awareness among pet owners and healthcare professionals to mitigate the risk of hookworm transmission to humans.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cytochrome-c oxidase</subject><subject>Dogs</subject><subject>Domestic animals</subject><subject>Genetic diversity</subject><subject>Haplotypes</subject><subject>Immunology</subject><subject>Infections</subject><subject>Larvae</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Parasites</subject><subject>Phylogeny</subject><subject>Public health</subject><subject>rRNA</subject><subject>rRNA 5.8S</subject><subject>Zoonoses</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kUtv1DAUhS0EokPhD7BAltiwIOBnHC9RxUsqYlPWluPczLgkvsVOQB3-PJ6ZAhILVr6yv3PusQ4hTzl7xRkzrwtjSrYNE6phHZeq2d8jG66kaLjV-j7ZMFtnxrk8I49KuWaMm1aph-RMdqLTXNgN-fkJJwjr5DONA6QljjH4JWKiPg10CwmWGOgQv0MucbmlONI9YsLD7Q7x6w_MM41phHAQlTrSAWcoRxVuCx0zzjRhXnbgywI5vaRXOx-nav-YPBj9VODJ3XlOvrx7e3Xxobn8_P7jxZvLJkijl6bvWwbGDKC85m1r6k-V73nbW6WNGa3pfDCdCHLoul4Jowwf26GXkhttlO7lOXlx8r3J-G2t2dwcS4CpZgBcixOWW6OU5aKiz_9Br3HNqaY7Uh2TyupKiRMVMpaSYXQ3Oc4-3zrO3KEad6rG1WrcsRq3r6Jnd9ZrP8PwR_K7iwrIE1DqU9pC_rv7P7a_ANKnm2E</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Rodpai, Rutchanee</creator><creator>Sanpool, Oranuch</creator><creator>Sadaow, Lakkhana</creator><creator>Boonroumkaew, Patcharaporn</creator><creator>Intapan, Pewpan M.</creator><creator>Maleewong, Wanchai</creator><creator>Yingklang, Manachai</creator><creator>Janwan, Penchom</creator><creator>Vaisusuk, Kotchaphon</creator><creator>Chatan, Wasupon</creator><creator>Piratae, Supawadee</creator><creator>Thanchomnang, Tongjit</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240201</creationdate><title>Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand</title><author>Rodpai, Rutchanee ; Sanpool, Oranuch ; Sadaow, Lakkhana ; Boonroumkaew, Patcharaporn ; Intapan, Pewpan M. ; Maleewong, Wanchai ; Yingklang, Manachai ; Janwan, Penchom ; Vaisusuk, Kotchaphon ; Chatan, Wasupon ; Piratae, Supawadee ; Thanchomnang, Tongjit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-bb60e77de4a516671344ab16b94577f978ac782c3d88b427471f6db33175745b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cytochrome-c oxidase</topic><topic>Dogs</topic><topic>Domestic animals</topic><topic>Genetic diversity</topic><topic>Haplotypes</topic><topic>Immunology</topic><topic>Infections</topic><topic>Larvae</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Parasites</topic><topic>Phylogeny</topic><topic>Public health</topic><topic>rRNA</topic><topic>rRNA 5.8S</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodpai, Rutchanee</creatorcontrib><creatorcontrib>Sanpool, Oranuch</creatorcontrib><creatorcontrib>Sadaow, Lakkhana</creatorcontrib><creatorcontrib>Boonroumkaew, Patcharaporn</creatorcontrib><creatorcontrib>Intapan, Pewpan M.</creatorcontrib><creatorcontrib>Maleewong, Wanchai</creatorcontrib><creatorcontrib>Yingklang, Manachai</creatorcontrib><creatorcontrib>Janwan, Penchom</creatorcontrib><creatorcontrib>Vaisusuk, Kotchaphon</creatorcontrib><creatorcontrib>Chatan, Wasupon</creatorcontrib><creatorcontrib>Piratae, Supawadee</creatorcontrib><creatorcontrib>Thanchomnang, Tongjit</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodpai, Rutchanee</au><au>Sanpool, Oranuch</au><au>Sadaow, Lakkhana</au><au>Boonroumkaew, Patcharaporn</au><au>Intapan, Pewpan M.</au><au>Maleewong, Wanchai</au><au>Yingklang, Manachai</au><au>Janwan, Penchom</au><au>Vaisusuk, Kotchaphon</au><au>Chatan, Wasupon</au><au>Piratae, Supawadee</au><au>Thanchomnang, Tongjit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2024-02-01</date><risdate>2024</risdate><volume>123</volume><issue>2</issue><spage>115</spage><epage>115</epage><pages>115-115</pages><artnum>115</artnum><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Hookworm infections remain a significant public health concern in tropical and subtropical regions, including Thailand. This study investigated the species and genetic diversity of hookworm infections in domestic dogs from northeastern Thailand. The molecular analysis focused on amplifying and sequencing specific regions of ribosomal RNA genes (ITS1-5.8S-ITS2 region) and the mitochondrial cytochrome c oxidase subunit 1 (
cox
1) gene in hookworm larvae recovered from 21 domestic dog stool samples. Among 21 larvae (one larva per infected dog) analyzed, 14 had sequences identical to
Ancylostoma caninum
, and 7 showed sequences almost identical to
Ancylostoma ceylanicum
. Phylogenetic analysis of
cox
1 sequences placed
A. caninum
and
A. ceylanicum
in separate clades. The median-joining network of
A. caninum cox
1 sequences from Thailand showed high haplotype diversity and belonged to the same cluster as sequences from Australia while forming separate clusters from those of
A. caninum
samples from the USA. The available published
A. ceylanicum cox
1 sequences (
n
= 33), in combination with seven sequences in the present study, represented 15 haplotypes distributed among three clusters. Interestingly,
A. ceylanicum
sequences from dogs and humans shared the same haplotypes. These findings are crucial for recognizing the potential for zoonotic transmission, highlighting the necessity for targeted control measures, and increasing awareness among pet owners and healthcare professionals to mitigate the risk of hookworm transmission to humans.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38285129</pmid><doi>10.1007/s00436-024-08134-z</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2024-02, Vol.123 (2), p.115-115, Article 115 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2919744912 |
| source | SpringerLink Journals |
| subjects | Biomedical and Life Sciences Biomedicine Cytochrome-c oxidase Dogs Domestic animals Genetic diversity Haplotypes Immunology Infections Larvae Medical Microbiology Microbiology Parasites Phylogeny Public health rRNA rRNA 5.8S Zoonoses |
| title | Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T01%3A20%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20identification%20and%20genetic%20diversity%20of%20zoonotic%20hookworm%20infections%20in%20domestic%20dogs%20from%20northeastern,%20Thailand&rft.jtitle=Parasitology%20research%20(1987)&rft.au=Rodpai,%20Rutchanee&rft.date=2024-02-01&rft.volume=123&rft.issue=2&rft.spage=115&rft.epage=115&rft.pages=115-115&rft.artnum=115&rft.issn=0932-0113&rft.eissn=1432-1955&rft_id=info:doi/10.1007/s00436-024-08134-z&rft_dat=%3Cproquest_cross%3E2919803495%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2919803495&rft_id=info:pmid/38285129&rfr_iscdi=true |