Polysaccharide of Asparagus cochinchinensis (Lour.) Merr regulates macrophage immune response and epigenetic memory through TLR4-JNK/p38/ERK signaling pathway and histone modification

Innate immune memory of macrophages is closely linked to histone modifications. While various studies have demonstrated that the polysaccharide of Asparagus cochinchinensis (Lour.) Merr (ACMP), extracted through alcohol-alkali extraction, enhances macrophages' non-specific immune function; no l...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Phytomedicine (Stuttgart) 2024-02, Vol.124, p.155294-155294, Article 155294
Hauptverfasser: Xie, Xiao-Dong, Tang, Min, Yi, Shou-Li, He, Ying, Chen, Si-Yu, Zhao, Yi, Chen, Qi, Cao, Mi-Xia, Yu, Mei-Ling, Wei, Ying-Yi, Yu, Wei-Hua, Hu, Ting-Jun
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 155294
container_issue
container_start_page 155294
container_title Phytomedicine (Stuttgart)
container_volume 124
creator Xie, Xiao-Dong
Tang, Min
Yi, Shou-Li
He, Ying
Chen, Si-Yu
Zhao, Yi
Chen, Qi
Cao, Mi-Xia
Yu, Mei-Ling
Wei, Ying-Yi
Yu, Wei-Hua
Hu, Ting-Jun
description Innate immune memory of macrophages is closely linked to histone modifications. While various studies have demonstrated that the polysaccharide of Asparagus cochinchinensis (Lour.) Merr (ACMP), extracted through alcohol-alkali extraction, enhances macrophages' non-specific immune function; no literature currently addresses whether ACMP's regulatory effect is related to innate immune memory and histone modification. This study aims to investigate if ACMP induces innate immune memory emergence in macrophages via pattern recognition receptor (PRR). After co-incubating different doses of ACMP with RAW264.7 cells and BMDM cells, we observed changes in signaling pathways related to PRR and assessed the presence of innate immune memory phenomenon in the cells. We observed the morphological characteristics of the ACMP using a scanning electron microscope, infrared spectrum, and HPLC pre-column derivatization method. We used q-PCR, Western blot, RNA-seq, and CUT&Tag-seq methods to examine ACMP's regulation of macrophage immune response and innate immune memory and explored its specific mechanism. ACMP, primarily composed of Man, GlcN, Rha, Fuc, GalA, Xyl, Glc, Gal, Ara, and, exhibited a molar ratio of each monosaccharide (1.41: 0.35: 0.49: 0.18: 1.00: 97.12: 0.36: 3.58: 1.14). ACMP regulated immunological function in macrophages through the TLR4-MAPK-JNK/p38/ERK pathway. ACMP induced elevated levels of chromosomal H3K4me1, enhancing TNF-α, IL-1β, and other genes' responsiveness, allowing macrophages to develop innate immune memory to ACMP stimulation. This study first time demonstrates that ACMP regulates immunological function through the TLR4-MAPK-JNK/ERK/p38 signaling pathway, distinct from prior reports. ACMP induces innate immune memory in macrophages in response to its immune stimulation by promoting increased H3K4me1 on chromosomes. This mechanism may be crucial in how plant polysaccharides regulate macrophages and the body's immune function.
doi_str_mv 10.1016/j.phymed.2023.155294
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2919744520</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2919744520</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-472394a6b546d6336e9eb9231581ebf9468f64abb95f3b32e213a30a97ba04143</originalsourceid><addsrcrecordid>eNo1kclu1EAQhlsIRIbAGyDUx3CwpzcvfYyisGVYFAWJm1Vul-0e2d2m2xbyk_F6eJjkUKrDv5RUHyFvOUs54_n-mE79OmKTCiZkyrNMaPWM7HjOy4Tp7NdzsmNaqaTgXF6QVzEeGeNKF-wluZAlL3JR8B35-8MPawRjegi2Qepbeh0nCNAtkRpveutOgy7aSK8Ofgnpe_oVQ6ABu2WAGSMdwQQ_9dAhteO4ONy0OHkXkYJrKE62Q4ezNXTE0YeVzn3wS9fTh8O9Sr58u9tPstzf3t_RaDsHg3UdnWDu_8D6v6C3cfZb6-gb21oDs_XuNXnRwhDxzeO-JD8_3D7cfEoO3z9-vrk-JEZmck5UIaRWkNeZyptcyhw11lpInpUc61arvGxzBXWts1bWUqDgEiQDXdTAFFfyklyde6fgfy8Y52q00eAwgEO_xEporgulMsE2qzpbt2_EGLCtpmBHCGvFWXVCVh2rM7LqhKw6I9ti7x4vLPVJewo9MZL_AE9yl0A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2919744520</pqid></control><display><type>article</type><title>Polysaccharide of Asparagus cochinchinensis (Lour.) Merr regulates macrophage immune response and epigenetic memory through TLR4-JNK/p38/ERK signaling pathway and histone modification</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Xie, Xiao-Dong ; Tang, Min ; Yi, Shou-Li ; He, Ying ; Chen, Si-Yu ; Zhao, Yi ; Chen, Qi ; Cao, Mi-Xia ; Yu, Mei-Ling ; Wei, Ying-Yi ; Yu, Wei-Hua ; Hu, Ting-Jun</creator><creatorcontrib>Xie, Xiao-Dong ; Tang, Min ; Yi, Shou-Li ; He, Ying ; Chen, Si-Yu ; Zhao, Yi ; Chen, Qi ; Cao, Mi-Xia ; Yu, Mei-Ling ; Wei, Ying-Yi ; Yu, Wei-Hua ; Hu, Ting-Jun</creatorcontrib><description>Innate immune memory of macrophages is closely linked to histone modifications. While various studies have demonstrated that the polysaccharide of Asparagus cochinchinensis (Lour.) Merr (ACMP), extracted through alcohol-alkali extraction, enhances macrophages' non-specific immune function; no literature currently addresses whether ACMP's regulatory effect is related to innate immune memory and histone modification. This study aims to investigate if ACMP induces innate immune memory emergence in macrophages via pattern recognition receptor (PRR). After co-incubating different doses of ACMP with RAW264.7 cells and BMDM cells, we observed changes in signaling pathways related to PRR and assessed the presence of innate immune memory phenomenon in the cells. We observed the morphological characteristics of the ACMP using a scanning electron microscope, infrared spectrum, and HPLC pre-column derivatization method. We used q-PCR, Western blot, RNA-seq, and CUT&amp;Tag-seq methods to examine ACMP's regulation of macrophage immune response and innate immune memory and explored its specific mechanism. ACMP, primarily composed of Man, GlcN, Rha, Fuc, GalA, Xyl, Glc, Gal, Ara, and, exhibited a molar ratio of each monosaccharide (1.41: 0.35: 0.49: 0.18: 1.00: 97.12: 0.36: 3.58: 1.14). ACMP regulated immunological function in macrophages through the TLR4-MAPK-JNK/p38/ERK pathway. ACMP induced elevated levels of chromosomal H3K4me1, enhancing TNF-α, IL-1β, and other genes' responsiveness, allowing macrophages to develop innate immune memory to ACMP stimulation. This study first time demonstrates that ACMP regulates immunological function through the TLR4-MAPK-JNK/ERK/p38 signaling pathway, distinct from prior reports. ACMP induces innate immune memory in macrophages in response to its immune stimulation by promoting increased H3K4me1 on chromosomes. This mechanism may be crucial in how plant polysaccharides regulate macrophages and the body's immune function.</description><identifier>ISSN: 0944-7113</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1016/j.phymed.2023.155294</identifier><identifier>PMID: 38176271</identifier><language>eng</language><publisher>Germany</publisher><ispartof>Phytomedicine (Stuttgart), 2024-02, Vol.124, p.155294-155294, Article 155294</ispartof><rights>Copyright © 2023 The Author(s). Published by Elsevier GmbH.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-472394a6b546d6336e9eb9231581ebf9468f64abb95f3b32e213a30a97ba04143</citedby><cites>FETCH-LOGICAL-c353t-472394a6b546d6336e9eb9231581ebf9468f64abb95f3b32e213a30a97ba04143</cites><orcidid>0009-0000-8423-897X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38176271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Xiao-Dong</creatorcontrib><creatorcontrib>Tang, Min</creatorcontrib><creatorcontrib>Yi, Shou-Li</creatorcontrib><creatorcontrib>He, Ying</creatorcontrib><creatorcontrib>Chen, Si-Yu</creatorcontrib><creatorcontrib>Zhao, Yi</creatorcontrib><creatorcontrib>Chen, Qi</creatorcontrib><creatorcontrib>Cao, Mi-Xia</creatorcontrib><creatorcontrib>Yu, Mei-Ling</creatorcontrib><creatorcontrib>Wei, Ying-Yi</creatorcontrib><creatorcontrib>Yu, Wei-Hua</creatorcontrib><creatorcontrib>Hu, Ting-Jun</creatorcontrib><title>Polysaccharide of Asparagus cochinchinensis (Lour.) Merr regulates macrophage immune response and epigenetic memory through TLR4-JNK/p38/ERK signaling pathway and histone modification</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>Innate immune memory of macrophages is closely linked to histone modifications. While various studies have demonstrated that the polysaccharide of Asparagus cochinchinensis (Lour.) Merr (ACMP), extracted through alcohol-alkali extraction, enhances macrophages' non-specific immune function; no literature currently addresses whether ACMP's regulatory effect is related to innate immune memory and histone modification. This study aims to investigate if ACMP induces innate immune memory emergence in macrophages via pattern recognition receptor (PRR). After co-incubating different doses of ACMP with RAW264.7 cells and BMDM cells, we observed changes in signaling pathways related to PRR and assessed the presence of innate immune memory phenomenon in the cells. We observed the morphological characteristics of the ACMP using a scanning electron microscope, infrared spectrum, and HPLC pre-column derivatization method. We used q-PCR, Western blot, RNA-seq, and CUT&amp;Tag-seq methods to examine ACMP's regulation of macrophage immune response and innate immune memory and explored its specific mechanism. ACMP, primarily composed of Man, GlcN, Rha, Fuc, GalA, Xyl, Glc, Gal, Ara, and, exhibited a molar ratio of each monosaccharide (1.41: 0.35: 0.49: 0.18: 1.00: 97.12: 0.36: 3.58: 1.14). ACMP regulated immunological function in macrophages through the TLR4-MAPK-JNK/p38/ERK pathway. ACMP induced elevated levels of chromosomal H3K4me1, enhancing TNF-α, IL-1β, and other genes' responsiveness, allowing macrophages to develop innate immune memory to ACMP stimulation. This study first time demonstrates that ACMP regulates immunological function through the TLR4-MAPK-JNK/ERK/p38 signaling pathway, distinct from prior reports. ACMP induces innate immune memory in macrophages in response to its immune stimulation by promoting increased H3K4me1 on chromosomes. This mechanism may be crucial in how plant polysaccharides regulate macrophages and the body's immune function.</description><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNo1kclu1EAQhlsIRIbAGyDUx3CwpzcvfYyisGVYFAWJm1Vul-0e2d2m2xbyk_F6eJjkUKrDv5RUHyFvOUs54_n-mE79OmKTCiZkyrNMaPWM7HjOy4Tp7NdzsmNaqaTgXF6QVzEeGeNKF-wluZAlL3JR8B35-8MPawRjegi2Qepbeh0nCNAtkRpveutOgy7aSK8Ofgnpe_oVQ6ABu2WAGSMdwQQ_9dAhteO4ONy0OHkXkYJrKE62Q4ezNXTE0YeVzn3wS9fTh8O9Sr58u9tPstzf3t_RaDsHg3UdnWDu_8D6v6C3cfZb6-gb21oDs_XuNXnRwhDxzeO-JD8_3D7cfEoO3z9-vrk-JEZmck5UIaRWkNeZyptcyhw11lpInpUc61arvGxzBXWts1bWUqDgEiQDXdTAFFfyklyde6fgfy8Y52q00eAwgEO_xEporgulMsE2qzpbt2_EGLCtpmBHCGvFWXVCVh2rM7LqhKw6I9ti7x4vLPVJewo9MZL_AE9yl0A</recordid><startdate>202402</startdate><enddate>202402</enddate><creator>Xie, Xiao-Dong</creator><creator>Tang, Min</creator><creator>Yi, Shou-Li</creator><creator>He, Ying</creator><creator>Chen, Si-Yu</creator><creator>Zhao, Yi</creator><creator>Chen, Qi</creator><creator>Cao, Mi-Xia</creator><creator>Yu, Mei-Ling</creator><creator>Wei, Ying-Yi</creator><creator>Yu, Wei-Hua</creator><creator>Hu, Ting-Jun</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0000-8423-897X</orcidid></search><sort><creationdate>202402</creationdate><title>Polysaccharide of Asparagus cochinchinensis (Lour.) Merr regulates macrophage immune response and epigenetic memory through TLR4-JNK/p38/ERK signaling pathway and histone modification</title><author>Xie, Xiao-Dong ; Tang, Min ; Yi, Shou-Li ; He, Ying ; Chen, Si-Yu ; Zhao, Yi ; Chen, Qi ; Cao, Mi-Xia ; Yu, Mei-Ling ; Wei, Ying-Yi ; Yu, Wei-Hua ; Hu, Ting-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-472394a6b546d6336e9eb9231581ebf9468f64abb95f3b32e213a30a97ba04143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Xiao-Dong</creatorcontrib><creatorcontrib>Tang, Min</creatorcontrib><creatorcontrib>Yi, Shou-Li</creatorcontrib><creatorcontrib>He, Ying</creatorcontrib><creatorcontrib>Chen, Si-Yu</creatorcontrib><creatorcontrib>Zhao, Yi</creatorcontrib><creatorcontrib>Chen, Qi</creatorcontrib><creatorcontrib>Cao, Mi-Xia</creatorcontrib><creatorcontrib>Yu, Mei-Ling</creatorcontrib><creatorcontrib>Wei, Ying-Yi</creatorcontrib><creatorcontrib>Yu, Wei-Hua</creatorcontrib><creatorcontrib>Hu, Ting-Jun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Xiao-Dong</au><au>Tang, Min</au><au>Yi, Shou-Li</au><au>He, Ying</au><au>Chen, Si-Yu</au><au>Zhao, Yi</au><au>Chen, Qi</au><au>Cao, Mi-Xia</au><au>Yu, Mei-Ling</au><au>Wei, Ying-Yi</au><au>Yu, Wei-Hua</au><au>Hu, Ting-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polysaccharide of Asparagus cochinchinensis (Lour.) Merr regulates macrophage immune response and epigenetic memory through TLR4-JNK/p38/ERK signaling pathway and histone modification</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2024-02</date><risdate>2024</risdate><volume>124</volume><spage>155294</spage><epage>155294</epage><pages>155294-155294</pages><artnum>155294</artnum><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>Innate immune memory of macrophages is closely linked to histone modifications. While various studies have demonstrated that the polysaccharide of Asparagus cochinchinensis (Lour.) Merr (ACMP), extracted through alcohol-alkali extraction, enhances macrophages' non-specific immune function; no literature currently addresses whether ACMP's regulatory effect is related to innate immune memory and histone modification. This study aims to investigate if ACMP induces innate immune memory emergence in macrophages via pattern recognition receptor (PRR). After co-incubating different doses of ACMP with RAW264.7 cells and BMDM cells, we observed changes in signaling pathways related to PRR and assessed the presence of innate immune memory phenomenon in the cells. We observed the morphological characteristics of the ACMP using a scanning electron microscope, infrared spectrum, and HPLC pre-column derivatization method. We used q-PCR, Western blot, RNA-seq, and CUT&amp;Tag-seq methods to examine ACMP's regulation of macrophage immune response and innate immune memory and explored its specific mechanism. ACMP, primarily composed of Man, GlcN, Rha, Fuc, GalA, Xyl, Glc, Gal, Ara, and, exhibited a molar ratio of each monosaccharide (1.41: 0.35: 0.49: 0.18: 1.00: 97.12: 0.36: 3.58: 1.14). ACMP regulated immunological function in macrophages through the TLR4-MAPK-JNK/p38/ERK pathway. ACMP induced elevated levels of chromosomal H3K4me1, enhancing TNF-α, IL-1β, and other genes' responsiveness, allowing macrophages to develop innate immune memory to ACMP stimulation. This study first time demonstrates that ACMP regulates immunological function through the TLR4-MAPK-JNK/ERK/p38 signaling pathway, distinct from prior reports. ACMP induces innate immune memory in macrophages in response to its immune stimulation by promoting increased H3K4me1 on chromosomes. This mechanism may be crucial in how plant polysaccharides regulate macrophages and the body's immune function.</abstract><cop>Germany</cop><pmid>38176271</pmid><doi>10.1016/j.phymed.2023.155294</doi><tpages>1</tpages><orcidid>https://orcid.org/0009-0000-8423-897X</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0944-7113
ispartof Phytomedicine (Stuttgart), 2024-02, Vol.124, p.155294-155294, Article 155294
issn 0944-7113
1618-095X
language eng
recordid cdi_proquest_miscellaneous_2919744520
source Elsevier ScienceDirect Journals Complete
title Polysaccharide of Asparagus cochinchinensis (Lour.) Merr regulates macrophage immune response and epigenetic memory through TLR4-JNK/p38/ERK signaling pathway and histone modification
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T20%3A09%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Polysaccharide%20of%20Asparagus%20cochinchinensis%20(Lour.)%20Merr%20regulates%20macrophage%20immune%20response%20and%20epigenetic%20memory%20through%20TLR4-JNK/p38/ERK%20signaling%20pathway%20and%20histone%20modification&rft.jtitle=Phytomedicine%20(Stuttgart)&rft.au=Xie,%20Xiao-Dong&rft.date=2024-02&rft.volume=124&rft.spage=155294&rft.epage=155294&rft.pages=155294-155294&rft.artnum=155294&rft.issn=0944-7113&rft.eissn=1618-095X&rft_id=info:doi/10.1016/j.phymed.2023.155294&rft_dat=%3Cproquest_cross%3E2919744520%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2919744520&rft_id=info:pmid/38176271&rfr_iscdi=true