Distribution of alpha‐synuclein in rat salivary glands
Expression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present...
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| Veröffentlicht in: | Anatomical record (Hoboken, N.J. : 2007) N.J. : 2007), 2024-08, Vol.307 (8), p.2933-2946 |
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| creator | Nishitani, Tomiko Mitoh, Yoshihiro Yajima, Takehiro Tachiya, Daisuke Hoshika, Tomohiro Fukunaga, Tomohiro Nishitani, Yoshihiro Yoshida, Ryusuke Mizoguchi, Itaru Ichikawa, Hiroyuki Sato, Tadasu |
| description | Expression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition. |
| doi_str_mv | 10.1002/ar.25395 |
| format | Article |
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The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition.</description><identifier>ISSN: 1932-8486</identifier><identifier>ISSN: 1932-8494</identifier><identifier>EISSN: 1932-8494</identifier><identifier>DOI: 10.1002/ar.25395</identifier><identifier>PMID: 38284507</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Acetyltransferase ; Actin ; alpha‐synuclein ; Aquaporin 5 ; choline acetyltransferase ; Choline O-acetyltransferase ; Confocal microscopy ; Exocrine glands ; Ganglia ; immunohistochemistry ; Lingual gland ; Lingual nerve ; Neurons ; Neurotransmitter release ; Parasympathetic nervous system ; Salivary gland ; Smooth muscle ; Spinal cord ; Submandibular gland ; Sympathetic nerves ; Synuclein ; Thorax ; Tyrosine 3-monooxygenase ; tyrosine hydroxylase</subject><ispartof>Anatomical record (Hoboken, N.J. : 2007), 2024-08, Vol.307 (8), p.2933-2946</ispartof><rights>2024 American Association for Anatomy.</rights><rights>2024 American Association for Anatomy</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3105-9c5f33c14b5bfa5c041d09859108f099ab45fea1dd6d1cc9c3e7c966485641f63</cites><orcidid>0000-0003-1132-680X ; 0000-0002-4679-7997</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Far.25395$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Far.25395$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38284507$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishitani, Tomiko</creatorcontrib><creatorcontrib>Mitoh, Yoshihiro</creatorcontrib><creatorcontrib>Yajima, Takehiro</creatorcontrib><creatorcontrib>Tachiya, Daisuke</creatorcontrib><creatorcontrib>Hoshika, Tomohiro</creatorcontrib><creatorcontrib>Fukunaga, Tomohiro</creatorcontrib><creatorcontrib>Nishitani, Yoshihiro</creatorcontrib><creatorcontrib>Yoshida, Ryusuke</creatorcontrib><creatorcontrib>Mizoguchi, Itaru</creatorcontrib><creatorcontrib>Ichikawa, Hiroyuki</creatorcontrib><creatorcontrib>Sato, Tadasu</creatorcontrib><title>Distribution of alpha‐synuclein in rat salivary glands</title><title>Anatomical record (Hoboken, N.J. : 2007)</title><addtitle>Anat Rec (Hoboken)</addtitle><description>Expression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition.</description><subject>Acetyltransferase</subject><subject>Actin</subject><subject>alpha‐synuclein</subject><subject>Aquaporin 5</subject><subject>choline acetyltransferase</subject><subject>Choline O-acetyltransferase</subject><subject>Confocal microscopy</subject><subject>Exocrine glands</subject><subject>Ganglia</subject><subject>immunohistochemistry</subject><subject>Lingual gland</subject><subject>Lingual nerve</subject><subject>Neurons</subject><subject>Neurotransmitter release</subject><subject>Parasympathetic nervous system</subject><subject>Salivary gland</subject><subject>Smooth muscle</subject><subject>Spinal cord</subject><subject>Submandibular gland</subject><subject>Sympathetic nerves</subject><subject>Synuclein</subject><subject>Thorax</subject><subject>Tyrosine 3-monooxygenase</subject><subject>tyrosine hydroxylase</subject><issn>1932-8486</issn><issn>1932-8494</issn><issn>1932-8494</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kMtKAzEUhoMoVqvgE8iAGzdTTyaXSZalXqEgiK5DJpNoynSmJjNKdz6Cz-iTONpaQRAOnLP4-PjPj9ARhhEGyM50GGWMSLaF9rAkWSqopNubW_AB2o9xBsAoSLKLBkRkgjLI95A497ENvuha39RJ4xJdLZ70x9t7XNadqayvk36CbpOoK_-iwzJ5rHRdxgO043QV7eF6D9HD5cX95Dqd3l7dTMbT1BAMLJWGOUIMpgUrnGYGKC5BCiYxCAdS6oIyZzUuS15iY6QhNjeScyoYp9hxMkSnK-8iNM-dja2a-2hs1YewTRdVJrHMKeFZ3qMnf9BZ04W6T6cI5FRIxoH8Ck1oYgzWqUXw8_4xhUF9tal0UN9t9ujxWtgVc1tuwJ_6eiBdAa--sst_RWp8txJ-Art1fI8</recordid><startdate>202408</startdate><enddate>202408</enddate><creator>Nishitani, Tomiko</creator><creator>Mitoh, Yoshihiro</creator><creator>Yajima, Takehiro</creator><creator>Tachiya, Daisuke</creator><creator>Hoshika, Tomohiro</creator><creator>Fukunaga, Tomohiro</creator><creator>Nishitani, Yoshihiro</creator><creator>Yoshida, Ryusuke</creator><creator>Mizoguchi, Itaru</creator><creator>Ichikawa, Hiroyuki</creator><creator>Sato, Tadasu</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1132-680X</orcidid><orcidid>https://orcid.org/0000-0002-4679-7997</orcidid></search><sort><creationdate>202408</creationdate><title>Distribution of alpha‐synuclein in rat salivary glands</title><author>Nishitani, Tomiko ; Mitoh, Yoshihiro ; Yajima, Takehiro ; Tachiya, Daisuke ; Hoshika, Tomohiro ; Fukunaga, Tomohiro ; Nishitani, Yoshihiro ; Yoshida, Ryusuke ; Mizoguchi, Itaru ; Ichikawa, Hiroyuki ; Sato, Tadasu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3105-9c5f33c14b5bfa5c041d09859108f099ab45fea1dd6d1cc9c3e7c966485641f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetyltransferase</topic><topic>Actin</topic><topic>alpha‐synuclein</topic><topic>Aquaporin 5</topic><topic>choline acetyltransferase</topic><topic>Choline O-acetyltransferase</topic><topic>Confocal microscopy</topic><topic>Exocrine glands</topic><topic>Ganglia</topic><topic>immunohistochemistry</topic><topic>Lingual gland</topic><topic>Lingual nerve</topic><topic>Neurons</topic><topic>Neurotransmitter release</topic><topic>Parasympathetic nervous system</topic><topic>Salivary gland</topic><topic>Smooth muscle</topic><topic>Spinal cord</topic><topic>Submandibular gland</topic><topic>Sympathetic nerves</topic><topic>Synuclein</topic><topic>Thorax</topic><topic>Tyrosine 3-monooxygenase</topic><topic>tyrosine hydroxylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishitani, Tomiko</creatorcontrib><creatorcontrib>Mitoh, Yoshihiro</creatorcontrib><creatorcontrib>Yajima, Takehiro</creatorcontrib><creatorcontrib>Tachiya, Daisuke</creatorcontrib><creatorcontrib>Hoshika, Tomohiro</creatorcontrib><creatorcontrib>Fukunaga, Tomohiro</creatorcontrib><creatorcontrib>Nishitani, Yoshihiro</creatorcontrib><creatorcontrib>Yoshida, Ryusuke</creatorcontrib><creatorcontrib>Mizoguchi, Itaru</creatorcontrib><creatorcontrib>Ichikawa, Hiroyuki</creatorcontrib><creatorcontrib>Sato, Tadasu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anatomical record (Hoboken, N.J. : 2007)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishitani, Tomiko</au><au>Mitoh, Yoshihiro</au><au>Yajima, Takehiro</au><au>Tachiya, Daisuke</au><au>Hoshika, Tomohiro</au><au>Fukunaga, Tomohiro</au><au>Nishitani, Yoshihiro</au><au>Yoshida, Ryusuke</au><au>Mizoguchi, Itaru</au><au>Ichikawa, Hiroyuki</au><au>Sato, Tadasu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of alpha‐synuclein in rat salivary glands</atitle><jtitle>Anatomical record (Hoboken, N.J. : 2007)</jtitle><addtitle>Anat Rec (Hoboken)</addtitle><date>2024-08</date><risdate>2024</risdate><volume>307</volume><issue>8</issue><spage>2933</spage><epage>2946</epage><pages>2933-2946</pages><issn>1932-8486</issn><issn>1932-8494</issn><eissn>1932-8494</eissn><abstract>Expression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>38284507</pmid><doi>10.1002/ar.25395</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-1132-680X</orcidid><orcidid>https://orcid.org/0000-0002-4679-7997</orcidid></addata></record> |
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| subjects | Acetyltransferase Actin alpha‐synuclein Aquaporin 5 choline acetyltransferase Choline O-acetyltransferase Confocal microscopy Exocrine glands Ganglia immunohistochemistry Lingual gland Lingual nerve Neurons Neurotransmitter release Parasympathetic nervous system Salivary gland Smooth muscle Spinal cord Submandibular gland Sympathetic nerves Synuclein Thorax Tyrosine 3-monooxygenase tyrosine hydroxylase |
| title | Distribution of alpha‐synuclein in rat salivary glands |
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