Transcription Factor SATB2 Regulates Skeletal Muscle Cell Proliferation and Migration via HDAC4 in Pigs

Skeletal muscle development remarkably affects meat production and growth rate, regulated by complex regulatory mechanisms in pigs. Specific AT sequence-binding protein 2 ( ) is a classic transcription factor and chromatin organizer, which holds a profound effect in the regulation of chromatin remodeling. However, the regulation role of concerning skeletal muscle cell fate through chromatin remodeling in pigs remains largely unknown. Here, we observed that was expressed higher in the lean-type compared to the obese-type pigs, which also enriched the pathways of skeletal muscle development, chromatin organization, and histone modification. Functionally, knockdown led to decreases in the proliferation and migration markers at the mRNA and protein expression levels, respectively, while overexpression had the opposite effects. Further, we found histone deacetylase 4 ( ) was a key downstream target gene of related to chromatin remodeling. The binding relationship between and was confirmed by a dual-luciferase reporter system and ChIP-qPCR analysis. Besides, we revealed that promoted the skeletal muscle cell proliferation and migration at the mRNA and protein expression levels, respectively. In conclusion, our study indicates that transcription factor binding to positively contributes to skeletal muscle cell proliferation and migration, which might mediate the chromatin remodeling to influence myogenesis in pigs. This study develops a novel insight into understanding the molecular regulatory mechanism of myogenesis, and provides a promising gene for genetic breeding in pigs.