A Comprehensive Analysis of Tn and STn Antigen Expression in Esophageal Adenocarcinoma
Differential glycosylation, marked by the presence of truncated O-glycans, is a distinctive feature of epithelial-derived cancers. However, there is a notable gap in research regarding the expression of Tn and STn antigens in esophageal adenocarcinoma (EAC). To address this, we employed commercially...
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| Veröffentlicht in: | Cancers 2024-01, Vol.16 (2), p.240 |
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| creator | Mercanoglu, Baris Karstens, Karl-Frederick Giannou, Anastasios D Meiners, Jan Lücke, Jöran Seeger, Philipp Brackrock, Vera Güngör, Cenap Izbicki, Jakob R Bockhorn, Maximilian Hackert, Thilo Melling, Nathaniel Wolters-Eisfeld, Gerrit |
| description | Differential glycosylation, marked by the presence of truncated O-glycans, is a distinctive feature of epithelial-derived cancers. However, there is a notable gap in research regarding the expression of Tn and STn antigens in esophageal adenocarcinoma (EAC). To address this, we employed commercially available antibodies, previously validated for Tn and STn antigens, to analyze two cohorts of EAC tissues. Initially, large-area tissue sections from formalin-fixed paraffin-embedded (FFPE) EAC and corresponding healthy tissues were subjected to immunohistochemistry (IHC) staining and scoring. Subsequently, we evaluated the RNA expression levels of crucial O-glycosylation related genes-
and
-using a quantitative real-time polymerase chain reaction (qRT-PCR). In a comprehensive analysis, a substantial cohort of EAC tissues (
= 311 for Tn antigen,
= 351 for STn antigen) was investigated and correlated with clinicopathological data. Our findings revealed that Tn and STn antigens are highly expressed (approximately 71% for both) in EAC, with this expression being tumor-specific. Notably, Tn antigen expression correlates significantly with the depth of tumor cell infiltration (
= 0.026). These antigens emerge as valuable markers and potential therapeutic targets for esophageal adenocarcinoma. |
| doi_str_mv | 10.3390/cancers16020240 |
| format | Article |
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and
-using a quantitative real-time polymerase chain reaction (qRT-PCR). In a comprehensive analysis, a substantial cohort of EAC tissues (
= 311 for Tn antigen,
= 351 for STn antigen) was investigated and correlated with clinicopathological data. Our findings revealed that Tn and STn antigens are highly expressed (approximately 71% for both) in EAC, with this expression being tumor-specific. Notably, Tn antigen expression correlates significantly with the depth of tumor cell infiltration (
= 0.026). These antigens emerge as valuable markers and potential therapeutic targets for esophageal adenocarcinoma.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers16020240</identifier><identifier>PMID: 38254730</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adenocarcinoma ; Analysis ; Antibodies ; Antigen (tumor-associated) ; Antigens ; B cells ; Bladder ; Cancer ; Cancer therapies ; Development and progression ; Esophageal cancer ; Esophagus ; Gastroesophageal reflux ; Genes ; Glycosylation ; Health aspects ; Immunohistochemistry ; Medical prognosis ; Metastases ; Metastasis ; Oncology, Experimental ; Ovarian cancer ; Patients ; Polymerase chain reaction ; Polysaccharides ; Prostate cancer ; Therapeutic targets ; Thoracic surgery ; Tumors ; Viral antibodies</subject><ispartof>Cancers, 2024-01, Vol.16 (2), p.240</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c387t-22c7b4f805e5fe849d4d03574330bef8be866732d09fa75e87bd8f473d8756b93</cites><orcidid>0000-0002-4498-6727 ; 0000-0002-5070-874X ; 0009-0004-7206-4297 ; 0000-0002-5173-9492 ; 0000-0002-2984-6515 ; 0000-0002-6081-479X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38254730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mercanoglu, Baris</creatorcontrib><creatorcontrib>Karstens, Karl-Frederick</creatorcontrib><creatorcontrib>Giannou, Anastasios D</creatorcontrib><creatorcontrib>Meiners, Jan</creatorcontrib><creatorcontrib>Lücke, Jöran</creatorcontrib><creatorcontrib>Seeger, Philipp</creatorcontrib><creatorcontrib>Brackrock, Vera</creatorcontrib><creatorcontrib>Güngör, Cenap</creatorcontrib><creatorcontrib>Izbicki, Jakob R</creatorcontrib><creatorcontrib>Bockhorn, Maximilian</creatorcontrib><creatorcontrib>Hackert, Thilo</creatorcontrib><creatorcontrib>Melling, Nathaniel</creatorcontrib><creatorcontrib>Wolters-Eisfeld, Gerrit</creatorcontrib><title>A Comprehensive Analysis of Tn and STn Antigen Expression in Esophageal Adenocarcinoma</title><title>Cancers</title><addtitle>Cancers (Basel)</addtitle><description>Differential glycosylation, marked by the presence of truncated O-glycans, is a distinctive feature of epithelial-derived cancers. However, there is a notable gap in research regarding the expression of Tn and STn antigens in esophageal adenocarcinoma (EAC). To address this, we employed commercially available antibodies, previously validated for Tn and STn antigens, to analyze two cohorts of EAC tissues. Initially, large-area tissue sections from formalin-fixed paraffin-embedded (FFPE) EAC and corresponding healthy tissues were subjected to immunohistochemistry (IHC) staining and scoring. Subsequently, we evaluated the RNA expression levels of crucial O-glycosylation related genes-
and
-using a quantitative real-time polymerase chain reaction (qRT-PCR). In a comprehensive analysis, a substantial cohort of EAC tissues (
= 311 for Tn antigen,
= 351 for STn antigen) was investigated and correlated with clinicopathological data. Our findings revealed that Tn and STn antigens are highly expressed (approximately 71% for both) in EAC, with this expression being tumor-specific. Notably, Tn antigen expression correlates significantly with the depth of tumor cell infiltration (
= 0.026). These antigens emerge as valuable markers and potential therapeutic targets for esophageal adenocarcinoma.</description><subject>Adenocarcinoma</subject><subject>Analysis</subject><subject>Antibodies</subject><subject>Antigen (tumor-associated)</subject><subject>Antigens</subject><subject>B cells</subject><subject>Bladder</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Development and progression</subject><subject>Esophageal cancer</subject><subject>Esophagus</subject><subject>Gastroesophageal reflux</subject><subject>Genes</subject><subject>Glycosylation</subject><subject>Health aspects</subject><subject>Immunohistochemistry</subject><subject>Medical prognosis</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Oncology, Experimental</subject><subject>Ovarian cancer</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Polysaccharides</subject><subject>Prostate cancer</subject><subject>Therapeutic targets</subject><subject>Thoracic surgery</subject><subject>Tumors</subject><subject>Viral antibodies</subject><issn>2072-6694</issn><issn>2072-6694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkUtPAyEUhYnR2EZduzMkbtxUGWAGZjlpfCUmLnxsJwxcWswMVGiN_nvxrY3cxeWS75xcchDaL8gxYzU50cpriKmoCCWUkw00pkTQSVXVfPPXfYT2Unog-TBWiEpsoxGTtOSCkTG6b_A0DIsIc_DJPQFuvOpfkks4WHzrsfIG3-Te-KWbgcenz5lNyQWPXZ5SWMzVDFSPGwM-aBW182FQu2jLqj7B3mffQXdnp7fTi8nV9fnltLmaaCbFckKpFh23kpRQWpC8NtwQVgrOGOnAyg5kVQlGDamtEiVI0Rlp8-ZGirLqaraDjj58FzE8riAt28ElDX2vPIRVamldiGxBhczo4Rr6EFYx__adkiUvWUV-qJnqoXXehmVU-s20bYQkUlDJeKaO_6FyGRicDh6sy-9_BCcfAh1DShFsu4huUPGlLUj7Fma7FmZWHHyuu-oGMN_8V3TsFfHcmFQ</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Mercanoglu, Baris</creator><creator>Karstens, Karl-Frederick</creator><creator>Giannou, Anastasios D</creator><creator>Meiners, Jan</creator><creator>Lücke, Jöran</creator><creator>Seeger, Philipp</creator><creator>Brackrock, Vera</creator><creator>Güngör, Cenap</creator><creator>Izbicki, Jakob R</creator><creator>Bockhorn, Maximilian</creator><creator>Hackert, Thilo</creator><creator>Melling, Nathaniel</creator><creator>Wolters-Eisfeld, Gerrit</creator><general>MDPI AG</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4498-6727</orcidid><orcidid>https://orcid.org/0000-0002-5070-874X</orcidid><orcidid>https://orcid.org/0009-0004-7206-4297</orcidid><orcidid>https://orcid.org/0000-0002-5173-9492</orcidid><orcidid>https://orcid.org/0000-0002-2984-6515</orcidid><orcidid>https://orcid.org/0000-0002-6081-479X</orcidid></search><sort><creationdate>20240101</creationdate><title>A Comprehensive Analysis of Tn and STn Antigen Expression in Esophageal Adenocarcinoma</title><author>Mercanoglu, Baris ; 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However, there is a notable gap in research regarding the expression of Tn and STn antigens in esophageal adenocarcinoma (EAC). To address this, we employed commercially available antibodies, previously validated for Tn and STn antigens, to analyze two cohorts of EAC tissues. Initially, large-area tissue sections from formalin-fixed paraffin-embedded (FFPE) EAC and corresponding healthy tissues were subjected to immunohistochemistry (IHC) staining and scoring. Subsequently, we evaluated the RNA expression levels of crucial O-glycosylation related genes-
and
-using a quantitative real-time polymerase chain reaction (qRT-PCR). In a comprehensive analysis, a substantial cohort of EAC tissues (
= 311 for Tn antigen,
= 351 for STn antigen) was investigated and correlated with clinicopathological data. Our findings revealed that Tn and STn antigens are highly expressed (approximately 71% for both) in EAC, with this expression being tumor-specific. Notably, Tn antigen expression correlates significantly with the depth of tumor cell infiltration (
= 0.026). These antigens emerge as valuable markers and potential therapeutic targets for esophageal adenocarcinoma.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38254730</pmid><doi>10.3390/cancers16020240</doi><orcidid>https://orcid.org/0000-0002-4498-6727</orcidid><orcidid>https://orcid.org/0000-0002-5070-874X</orcidid><orcidid>https://orcid.org/0009-0004-7206-4297</orcidid><orcidid>https://orcid.org/0000-0002-5173-9492</orcidid><orcidid>https://orcid.org/0000-0002-2984-6515</orcidid><orcidid>https://orcid.org/0000-0002-6081-479X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma Analysis Antibodies Antigen (tumor-associated) Antigens B cells Bladder Cancer Cancer therapies Development and progression Esophageal cancer Esophagus Gastroesophageal reflux Genes Glycosylation Health aspects Immunohistochemistry Medical prognosis Metastases Metastasis Oncology, Experimental Ovarian cancer Patients Polymerase chain reaction Polysaccharides Prostate cancer Therapeutic targets Thoracic surgery Tumors Viral antibodies |
| title | A Comprehensive Analysis of Tn and STn Antigen Expression in Esophageal Adenocarcinoma |
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