Evaluation of the potential of endophytic Trichoderma sp. isolated from medicinal plant Ampelopsis japonica against MRSA and bioassay-guided separation of the anti-MRSA compound
Endophytic fungi have been recognized as a valuable source for the production of biologically active compounds with potential applications in various domains. This study aimed to isolate endophytic fungi from Ampelopsis japonica (Thunb.) Makino and assess their anti-MRSA activity. Meanwhile, chromat...
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| Veröffentlicht in: | Brazilian journal of microbiology 2024-03, Vol.55 (1), p.543-556 |
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| creator | Li, Jianbin Xie, Siyun Gao, Qing Deng, Zujun |
| description | Endophytic fungi have been recognized as a valuable source for the production of biologically active compounds with potential applications in various domains. This study aimed to isolate endophytic fungi from
Ampelopsis japonica
(Thunb.) Makino and assess their anti-MRSA activity. Meanwhile, chromatographic separation techniques were applied to analyze the constituents of endophytic fungal secondary metabolites. The isolate BLR24, which exhibited strong inhibition activity against MRSA, was identified as
Trichoderma virens
based on morphological characteristics and ITS sequence analyses. The ethyl acetate extract of BLR24 (EA-BLR24) showed good anti-MRSA activity with the MIC and MBC values of 25 μg/mL and 50 μg/mL, separately. The inhibition of biofilm formation was up to 34.67% under MIC concentration treatment. Meanwhile, EA-BLR24 could significantly reduce the expression of biofilm-related genes (
icaA
,
sarA
, and
agrA
) of MRSA. Based on LC-MS/MS analysis, twenty compounds in EA-BLR24 could be annotated using the GNPS platform, mainly diketopiperazines. The anti-MRSA compound (Fr.1.1) was obtained from EA-BLR24 by bioassay-guided fractionation and determined as gliotoxin. The results indicated that endophytic
Trichoderma virens
BLR24 isolated from the medical plant
A. japonica
roots could be a promising source of natural anti-MRSA agents. Endophytic fungal secondary metabolites are abundant in biologically active compounds. Endophytic fungi from medicinal plants could be a source yielding bioactive metabolites of pharmaceutical importance. |
| doi_str_mv | 10.1007/s42770-024-01250-z |
| format | Article |
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Ampelopsis japonica
(Thunb.) Makino and assess their anti-MRSA activity. Meanwhile, chromatographic separation techniques were applied to analyze the constituents of endophytic fungal secondary metabolites. The isolate BLR24, which exhibited strong inhibition activity against MRSA, was identified as
Trichoderma virens
based on morphological characteristics and ITS sequence analyses. The ethyl acetate extract of BLR24 (EA-BLR24) showed good anti-MRSA activity with the MIC and MBC values of 25 μg/mL and 50 μg/mL, separately. The inhibition of biofilm formation was up to 34.67% under MIC concentration treatment. Meanwhile, EA-BLR24 could significantly reduce the expression of biofilm-related genes (
icaA
,
sarA
, and
agrA
) of MRSA. Based on LC-MS/MS analysis, twenty compounds in EA-BLR24 could be annotated using the GNPS platform, mainly diketopiperazines. The anti-MRSA compound (Fr.1.1) was obtained from EA-BLR24 by bioassay-guided fractionation and determined as gliotoxin. The results indicated that endophytic
Trichoderma virens
BLR24 isolated from the medical plant
A. japonica
roots could be a promising source of natural anti-MRSA agents. Endophytic fungal secondary metabolites are abundant in biologically active compounds. Endophytic fungi from medicinal plants could be a source yielding bioactive metabolites of pharmaceutical importance.</description><identifier>ISSN: 1517-8382</identifier><identifier>ISSN: 1678-4405</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-024-01250-z</identifier><identifier>PMID: 38261262</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Acetic acid ; Ampelopsis ; Ampelopsis japonica ; Bioactive compounds ; Bioassays ; Biofilms ; Biological activity ; Biomedical and Life Sciences ; Chromatography, Liquid ; Drug resistance ; Endophytes ; Environmental Microbiology - Research Paper ; Ethyl acetate ; Food Microbiology ; Fractionation ; Fungi ; Gene expression ; Gliotoxin ; Herbal medicine ; Life Sciences ; Medical Microbiology ; Medicinal plants ; Metabolites ; Methicillin-Resistant Staphylococcus aureus ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Minimum inhibitory concentration ; Mycology ; Physical characteristics ; Plants, Medicinal ; Secondary metabolites ; Separation ; Separation techniques ; Tandem Mass Spectrometry ; Trichoderma ; Trichoderma virens</subject><ispartof>Brazilian journal of microbiology, 2024-03, Vol.55 (1), p.543-556</ispartof><rights>The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-8c5089f2ba0ea4f4ddb31c8aa7db60a68787a11e85cc9d47accd5e6ac666eefc3</cites><orcidid>0009-0000-7729-6725 ; 0000-0002-6704-4281</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s42770-024-01250-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s42770-024-01250-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38261262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jianbin</creatorcontrib><creatorcontrib>Xie, Siyun</creatorcontrib><creatorcontrib>Gao, Qing</creatorcontrib><creatorcontrib>Deng, Zujun</creatorcontrib><title>Evaluation of the potential of endophytic Trichoderma sp. isolated from medicinal plant Ampelopsis japonica against MRSA and bioassay-guided separation of the anti-MRSA compound</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Endophytic fungi have been recognized as a valuable source for the production of biologically active compounds with potential applications in various domains. This study aimed to isolate endophytic fungi from
Ampelopsis japonica
(Thunb.) Makino and assess their anti-MRSA activity. Meanwhile, chromatographic separation techniques were applied to analyze the constituents of endophytic fungal secondary metabolites. The isolate BLR24, which exhibited strong inhibition activity against MRSA, was identified as
Trichoderma virens
based on morphological characteristics and ITS sequence analyses. The ethyl acetate extract of BLR24 (EA-BLR24) showed good anti-MRSA activity with the MIC and MBC values of 25 μg/mL and 50 μg/mL, separately. The inhibition of biofilm formation was up to 34.67% under MIC concentration treatment. Meanwhile, EA-BLR24 could significantly reduce the expression of biofilm-related genes (
icaA
,
sarA
, and
agrA
) of MRSA. Based on LC-MS/MS analysis, twenty compounds in EA-BLR24 could be annotated using the GNPS platform, mainly diketopiperazines. The anti-MRSA compound (Fr.1.1) was obtained from EA-BLR24 by bioassay-guided fractionation and determined as gliotoxin. The results indicated that endophytic
Trichoderma virens
BLR24 isolated from the medical plant
A. japonica
roots could be a promising source of natural anti-MRSA agents. Endophytic fungal secondary metabolites are abundant in biologically active compounds. Endophytic fungi from medicinal plants could be a source yielding bioactive metabolites of pharmaceutical importance.</description><subject>Acetic acid</subject><subject>Ampelopsis</subject><subject>Ampelopsis japonica</subject><subject>Bioactive compounds</subject><subject>Bioassays</subject><subject>Biofilms</subject><subject>Biological activity</subject><subject>Biomedical and Life Sciences</subject><subject>Chromatography, Liquid</subject><subject>Drug resistance</subject><subject>Endophytes</subject><subject>Environmental Microbiology - Research Paper</subject><subject>Ethyl acetate</subject><subject>Food Microbiology</subject><subject>Fractionation</subject><subject>Fungi</subject><subject>Gene expression</subject><subject>Gliotoxin</subject><subject>Herbal medicine</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Medicinal plants</subject><subject>Metabolites</subject><subject>Methicillin-Resistant Staphylococcus aureus</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Minimum inhibitory concentration</subject><subject>Mycology</subject><subject>Physical characteristics</subject><subject>Plants, Medicinal</subject><subject>Secondary metabolites</subject><subject>Separation</subject><subject>Separation techniques</subject><subject>Tandem Mass Spectrometry</subject><subject>Trichoderma</subject><subject>Trichoderma virens</subject><issn>1517-8382</issn><issn>1678-4405</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd9uFCEYxSdGY2v1BbwwJN54QwWGAfZy09Q_SY2J1uvJN8DsspkBBKbJ9q36hmW7VaMXXkE-fucA5zTNa0rOKSHyfeZMSoIJ45hQ1hF8-6Q5pUIqzDnpntZ9RyVWrWInzYucd4RUiLPnzUkdCcoEO23uLm9gWqC44FEYUdlaFEOxvjiYDgPrTYjbfXEaXSent8HYNAPK8Ry5HCYo1qAxhRnN1jjtfFXFCXxB6znaKcTsMtpBDN5pQLAB53NBX759XyPwBg0uQM6wx5vFmeqUbYT012OqlcMPvA5zDIs3L5tnI0zZvnpcz5ofHy6vLz7hq68fP1-sr7BumShY6Y6o1cgGIBb4yI0ZWqoVgDSDICCUVBIotarTemW4BK1NZwVoIYS1o27PmndH35jCz8Xm0s8uazvV39mw5J6tqFSiRswq-vYfdBeWVLM4UJwxwlcdrRQ7UjqFnJMd-5jcDGnfU9IfCu2Phfa10P6h0P62it48Wi9Djfi35FeDFWiPQK5HfmPTn7v_Y3sPZeqwRw</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Li, Jianbin</creator><creator>Xie, Siyun</creator><creator>Gao, Qing</creator><creator>Deng, Zujun</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0000-7729-6725</orcidid><orcidid>https://orcid.org/0000-0002-6704-4281</orcidid></search><sort><creationdate>20240301</creationdate><title>Evaluation of the potential of endophytic Trichoderma sp. isolated from medicinal plant Ampelopsis japonica against MRSA and bioassay-guided separation of the anti-MRSA compound</title><author>Li, Jianbin ; Xie, Siyun ; Gao, Qing ; Deng, Zujun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-8c5089f2ba0ea4f4ddb31c8aa7db60a68787a11e85cc9d47accd5e6ac666eefc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetic acid</topic><topic>Ampelopsis</topic><topic>Ampelopsis japonica</topic><topic>Bioactive compounds</topic><topic>Bioassays</topic><topic>Biofilms</topic><topic>Biological activity</topic><topic>Biomedical and Life Sciences</topic><topic>Chromatography, Liquid</topic><topic>Drug resistance</topic><topic>Endophytes</topic><topic>Environmental Microbiology - Research Paper</topic><topic>Ethyl acetate</topic><topic>Food Microbiology</topic><topic>Fractionation</topic><topic>Fungi</topic><topic>Gene expression</topic><topic>Gliotoxin</topic><topic>Herbal medicine</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Medicinal plants</topic><topic>Metabolites</topic><topic>Methicillin-Resistant Staphylococcus aureus</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Minimum inhibitory concentration</topic><topic>Mycology</topic><topic>Physical characteristics</topic><topic>Plants, Medicinal</topic><topic>Secondary metabolites</topic><topic>Separation</topic><topic>Separation techniques</topic><topic>Tandem Mass Spectrometry</topic><topic>Trichoderma</topic><topic>Trichoderma virens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jianbin</creatorcontrib><creatorcontrib>Xie, Siyun</creatorcontrib><creatorcontrib>Gao, Qing</creatorcontrib><creatorcontrib>Deng, Zujun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jianbin</au><au>Xie, Siyun</au><au>Gao, Qing</au><au>Deng, Zujun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the potential of endophytic Trichoderma sp. isolated from medicinal plant Ampelopsis japonica against MRSA and bioassay-guided separation of the anti-MRSA compound</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>55</volume><issue>1</issue><spage>543</spage><epage>556</epage><pages>543-556</pages><issn>1517-8382</issn><issn>1678-4405</issn><eissn>1678-4405</eissn><abstract>Endophytic fungi have been recognized as a valuable source for the production of biologically active compounds with potential applications in various domains. This study aimed to isolate endophytic fungi from
Ampelopsis japonica
(Thunb.) Makino and assess their anti-MRSA activity. Meanwhile, chromatographic separation techniques were applied to analyze the constituents of endophytic fungal secondary metabolites. The isolate BLR24, which exhibited strong inhibition activity against MRSA, was identified as
Trichoderma virens
based on morphological characteristics and ITS sequence analyses. The ethyl acetate extract of BLR24 (EA-BLR24) showed good anti-MRSA activity with the MIC and MBC values of 25 μg/mL and 50 μg/mL, separately. The inhibition of biofilm formation was up to 34.67% under MIC concentration treatment. Meanwhile, EA-BLR24 could significantly reduce the expression of biofilm-related genes (
icaA
,
sarA
, and
agrA
) of MRSA. Based on LC-MS/MS analysis, twenty compounds in EA-BLR24 could be annotated using the GNPS platform, mainly diketopiperazines. The anti-MRSA compound (Fr.1.1) was obtained from EA-BLR24 by bioassay-guided fractionation and determined as gliotoxin. The results indicated that endophytic
Trichoderma virens
BLR24 isolated from the medical plant
A. japonica
roots could be a promising source of natural anti-MRSA agents. Endophytic fungal secondary metabolites are abundant in biologically active compounds. Endophytic fungi from medicinal plants could be a source yielding bioactive metabolites of pharmaceutical importance.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>38261262</pmid><doi>10.1007/s42770-024-01250-z</doi><tpages>14</tpages><orcidid>https://orcid.org/0009-0000-7729-6725</orcidid><orcidid>https://orcid.org/0000-0002-6704-4281</orcidid></addata></record> |
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| subjects | Acetic acid Ampelopsis Ampelopsis japonica Bioactive compounds Bioassays Biofilms Biological activity Biomedical and Life Sciences Chromatography, Liquid Drug resistance Endophytes Environmental Microbiology - Research Paper Ethyl acetate Food Microbiology Fractionation Fungi Gene expression Gliotoxin Herbal medicine Life Sciences Medical Microbiology Medicinal plants Metabolites Methicillin-Resistant Staphylococcus aureus Microbial Ecology Microbial Genetics and Genomics Microbiology Minimum inhibitory concentration Mycology Physical characteristics Plants, Medicinal Secondary metabolites Separation Separation techniques Tandem Mass Spectrometry Trichoderma Trichoderma virens |
| title | Evaluation of the potential of endophytic Trichoderma sp. isolated from medicinal plant Ampelopsis japonica against MRSA and bioassay-guided separation of the anti-MRSA compound |
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