Engineering of methionine sulfoxide reductase A with simultaneously improved stability and activity for kinetic resolution of chiral sulfoxides

Methionine sulfoxide reductase A (MsrA) has emerged as promising biocatalysts in the enantioselective kinetic resolution of racemic (rac) sulfoxides. In this study, we engineered robust MsrA variants through directed evolution, demonstrating substantial improvements of thermostability. Mechanism ana...

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Veröffentlicht in:	International journal of biological macromolecules 2024-03, Vol.260 (Pt 1), p.129540-129540, Article 129540
Hauptverfasser:	Zhang, Quan, Pan, Bochen, Yang, Piao, Tian, Jin, Zhou, Shihuan, Xu, Xianlin, Dai, Yangxue, Cheng, Xiaoling, Chen, Yongzheng, Yang, Jiawei
Format:	Artikel
Sprache:	eng
Schlagworte:	Activity Chiral sulfoxide Methionine sulfoxide reductase A Substrate tolerance Thermostability
Online-Zugang:	Volltext
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