Genetic polymorphism and clustering of the Plasmodium cynomolgi Duffy binding protein 1 region II of recent macaque isolates from Peninsular Malaysia
Plasmodium cynomolgi is a simian malaria parasite that has been increasingly infecting humans. It is naturally present in the long-tailed and pig-tailed macaques in Southeast Asia. The P. cynomolgi Duffy binding protein 1 region II [PcDBP1(II)] plays an essential role in the invasion of the parasite...
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| Veröffentlicht in: | Parasitology research (1987) 2024-01, Vol.123 (1), p.105-105, Article 105 |
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| container_title | Parasitology research (1987) |
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| creator | Latif, Eira Nurfarisha Mohd Noordin, Naqib Rafieqin Shahari, Shahhaziq Amir, Amirah Lau, Yee-Ling Cheong, Fei-Wen Abdullah, Mohd Lutfi Fong, Mun Yik |
| description | Plasmodium cynomolgi
is a simian malaria parasite that has been increasingly infecting humans. It is naturally present in the long-tailed and pig-tailed macaques in Southeast Asia. The
P. cynomolgi
Duffy binding protein 1 region II [PcDBP1(II)] plays an essential role in the invasion of the parasite into host erythrocytes. This study investigated the genetic polymorphism, natural selection and haplotype clustering of PcDBP1(II) from wild macaque isolates in Peninsular Malaysia. The genomic DNA of 50
P. cynomolgi
isolates was extracted from the macaque blood samples. Their
PcDBP1(II)
gene was amplified using a semi-nested PCR, cloned into a plasmid vector and subsequently sequenced. The polymorphism, natural selection and haplotypes of PcDBP1(II) were analysed using MEGA X and DnaSP ver.6.12.03 programmes. The analyses revealed high genetic polymorphism of PcDBP1(II) (
π
= 0.026 ± 0.004;
H
d
= 0.996 ± 0.001), and it was under purifying (negative) selection. A total of 106 haplotypes of PcDBP1(II) were identified. Phylogenetic and haplotype analyses revealed two groups of PcDBP1(II). Amino acid length polymorphism was observed between the groups, which may lead to possible phenotypic difference between them. |
| doi_str_mv | 10.1007/s00436-024-08125-0 |
| format | Article |
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is a simian malaria parasite that has been increasingly infecting humans. It is naturally present in the long-tailed and pig-tailed macaques in Southeast Asia. The
P. cynomolgi
Duffy binding protein 1 region II [PcDBP1(II)] plays an essential role in the invasion of the parasite into host erythrocytes. This study investigated the genetic polymorphism, natural selection and haplotype clustering of PcDBP1(II) from wild macaque isolates in Peninsular Malaysia. The genomic DNA of 50
P. cynomolgi
isolates was extracted from the macaque blood samples. Their
PcDBP1(II)
gene was amplified using a semi-nested PCR, cloned into a plasmid vector and subsequently sequenced. The polymorphism, natural selection and haplotypes of PcDBP1(II) were analysed using MEGA X and DnaSP ver.6.12.03 programmes. The analyses revealed high genetic polymorphism of PcDBP1(II) (
π
= 0.026 ± 0.004;
H
d
= 0.996 ± 0.001), and it was under purifying (negative) selection. A total of 106 haplotypes of PcDBP1(II) were identified. Phylogenetic and haplotype analyses revealed two groups of PcDBP1(II). Amino acid length polymorphism was observed between the groups, which may lead to possible phenotypic difference between them.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-024-08125-0</identifier><identifier>PMID: 38240877</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Amino acids ; Biomedical and Life Sciences ; Biomedicine ; DNA ; Erythrocytes ; Gene polymorphism ; genes ; Genetic analysis ; Genetic aspects ; genetic polymorphism ; Genetic polymorphisms ; Genetic vectors ; Haplotypes ; Immunology ; Malaria ; Malaysia ; Medical Microbiology ; Microbiology ; Natural selection ; parasites ; phenotype ; Phylogeny ; plasmid vectors ; Plasmodium cynomolgi ; Plasmodium falciparum ; Polymorphism ; Protein binding</subject><ispartof>Parasitology research (1987), 2024-01, Vol.123 (1), p.105-105, Article 105</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>COPYRIGHT 2024 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c426t-27f2e6939b2934d2c17286727813d50d7f0b88519ee74eacff2e9a2b1c51d11a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-024-08125-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-024-08125-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38240877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Latif, Eira Nurfarisha Mohd</creatorcontrib><creatorcontrib>Noordin, Naqib Rafieqin</creatorcontrib><creatorcontrib>Shahari, Shahhaziq</creatorcontrib><creatorcontrib>Amir, Amirah</creatorcontrib><creatorcontrib>Lau, Yee-Ling</creatorcontrib><creatorcontrib>Cheong, Fei-Wen</creatorcontrib><creatorcontrib>Abdullah, Mohd Lutfi</creatorcontrib><creatorcontrib>Fong, Mun Yik</creatorcontrib><title>Genetic polymorphism and clustering of the Plasmodium cynomolgi Duffy binding protein 1 region II of recent macaque isolates from Peninsular Malaysia</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Plasmodium cynomolgi
is a simian malaria parasite that has been increasingly infecting humans. It is naturally present in the long-tailed and pig-tailed macaques in Southeast Asia. The
P. cynomolgi
Duffy binding protein 1 region II [PcDBP1(II)] plays an essential role in the invasion of the parasite into host erythrocytes. This study investigated the genetic polymorphism, natural selection and haplotype clustering of PcDBP1(II) from wild macaque isolates in Peninsular Malaysia. The genomic DNA of 50
P. cynomolgi
isolates was extracted from the macaque blood samples. Their
PcDBP1(II)
gene was amplified using a semi-nested PCR, cloned into a plasmid vector and subsequently sequenced. The polymorphism, natural selection and haplotypes of PcDBP1(II) were analysed using MEGA X and DnaSP ver.6.12.03 programmes. The analyses revealed high genetic polymorphism of PcDBP1(II) (
π
= 0.026 ± 0.004;
H
d
= 0.996 ± 0.001), and it was under purifying (negative) selection. A total of 106 haplotypes of PcDBP1(II) were identified. Phylogenetic and haplotype analyses revealed two groups of PcDBP1(II). Amino acid length polymorphism was observed between the groups, which may lead to possible phenotypic difference between them.</description><subject>Amino acids</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>DNA</subject><subject>Erythrocytes</subject><subject>Gene polymorphism</subject><subject>genes</subject><subject>Genetic analysis</subject><subject>Genetic aspects</subject><subject>genetic polymorphism</subject><subject>Genetic polymorphisms</subject><subject>Genetic vectors</subject><subject>Haplotypes</subject><subject>Immunology</subject><subject>Malaria</subject><subject>Malaysia</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Natural selection</subject><subject>parasites</subject><subject>phenotype</subject><subject>Phylogeny</subject><subject>plasmid vectors</subject><subject>Plasmodium cynomolgi</subject><subject>Plasmodium falciparum</subject><subject>Polymorphism</subject><subject>Protein binding</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFks1u1DAUhSMEosPAC7BAltiwSfFfYmdZtbSMVEQXsLY8zvXUlX8GO1nMg_C-OJ1CBUIgL2xdfefoXOs0zWuCTwnG4n3BmLO-xZS3WBLatfhJsyKc0ZYMXfe0WeGhvjEh7KR5UcodxkT0nD9vTpikHEshVs33K4gwOYP2yR9CyvtbVwLScUTGz2WC7OIOJYumW0A3XpeQRjcHZA4xheR3Dl3M1h7Q1sVxIfc5TeAiIijDzqWINptFncFAnFDQRn-bAbmSvJ6gIJtTQDcQXSyz1xl90l4fitMvm2dW-wKvHu518_Xyw5fzj-3156vN-dl1azjtp5YKS6Ef2LClA-MjNURQ2QsqJGFjh0dh8VbKjgwAgoM2tuKDpltiOjISotm6eXf0rblrsDKp4IoB73WENBfFSMd6LJig_0XpQETXcVr_fN28_QO9S3OOdZGF6nvZy6F7pHbag3LRpilrs5iqMyEGKRmnolKnf6HqGSE4kyJYV-e_CehRYHIqJYNV--yCzgdFsFpqo461UbU26r42ClfRm4fE8zbA-EvysycVYEeg7JdGQH5c6R-2PwBAAMzH</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Latif, Eira Nurfarisha Mohd</creator><creator>Noordin, Naqib Rafieqin</creator><creator>Shahari, Shahhaziq</creator><creator>Amir, Amirah</creator><creator>Lau, Yee-Ling</creator><creator>Cheong, Fei-Wen</creator><creator>Abdullah, Mohd Lutfi</creator><creator>Fong, Mun Yik</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240101</creationdate><title>Genetic polymorphism and clustering of the Plasmodium cynomolgi Duffy binding protein 1 region II of recent macaque isolates from Peninsular Malaysia</title><author>Latif, Eira Nurfarisha Mohd ; Noordin, Naqib Rafieqin ; Shahari, Shahhaziq ; Amir, Amirah ; Lau, Yee-Ling ; Cheong, Fei-Wen ; Abdullah, Mohd Lutfi ; Fong, Mun Yik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-27f2e6939b2934d2c17286727813d50d7f0b88519ee74eacff2e9a2b1c51d11a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amino acids</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>DNA</topic><topic>Erythrocytes</topic><topic>Gene polymorphism</topic><topic>genes</topic><topic>Genetic analysis</topic><topic>Genetic aspects</topic><topic>genetic polymorphism</topic><topic>Genetic polymorphisms</topic><topic>Genetic vectors</topic><topic>Haplotypes</topic><topic>Immunology</topic><topic>Malaria</topic><topic>Malaysia</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Natural selection</topic><topic>parasites</topic><topic>phenotype</topic><topic>Phylogeny</topic><topic>plasmid vectors</topic><topic>Plasmodium cynomolgi</topic><topic>Plasmodium falciparum</topic><topic>Polymorphism</topic><topic>Protein binding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Latif, Eira Nurfarisha Mohd</creatorcontrib><creatorcontrib>Noordin, Naqib Rafieqin</creatorcontrib><creatorcontrib>Shahari, Shahhaziq</creatorcontrib><creatorcontrib>Amir, Amirah</creatorcontrib><creatorcontrib>Lau, Yee-Ling</creatorcontrib><creatorcontrib>Cheong, Fei-Wen</creatorcontrib><creatorcontrib>Abdullah, Mohd Lutfi</creatorcontrib><creatorcontrib>Fong, Mun Yik</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Latif, Eira Nurfarisha Mohd</au><au>Noordin, Naqib Rafieqin</au><au>Shahari, Shahhaziq</au><au>Amir, Amirah</au><au>Lau, Yee-Ling</au><au>Cheong, Fei-Wen</au><au>Abdullah, Mohd Lutfi</au><au>Fong, Mun Yik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic polymorphism and clustering of the Plasmodium cynomolgi Duffy binding protein 1 region II of recent macaque isolates from Peninsular Malaysia</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>123</volume><issue>1</issue><spage>105</spage><epage>105</epage><pages>105-105</pages><artnum>105</artnum><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Plasmodium cynomolgi
is a simian malaria parasite that has been increasingly infecting humans. It is naturally present in the long-tailed and pig-tailed macaques in Southeast Asia. The
P. cynomolgi
Duffy binding protein 1 region II [PcDBP1(II)] plays an essential role in the invasion of the parasite into host erythrocytes. This study investigated the genetic polymorphism, natural selection and haplotype clustering of PcDBP1(II) from wild macaque isolates in Peninsular Malaysia. The genomic DNA of 50
P. cynomolgi
isolates was extracted from the macaque blood samples. Their
PcDBP1(II)
gene was amplified using a semi-nested PCR, cloned into a plasmid vector and subsequently sequenced. The polymorphism, natural selection and haplotypes of PcDBP1(II) were analysed using MEGA X and DnaSP ver.6.12.03 programmes. The analyses revealed high genetic polymorphism of PcDBP1(II) (
π
= 0.026 ± 0.004;
H
d
= 0.996 ± 0.001), and it was under purifying (negative) selection. A total of 106 haplotypes of PcDBP1(II) were identified. Phylogenetic and haplotype analyses revealed two groups of PcDBP1(II). Amino acid length polymorphism was observed between the groups, which may lead to possible phenotypic difference between them.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38240877</pmid><doi>10.1007/s00436-024-08125-0</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2024-01, Vol.123 (1), p.105-105, Article 105 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2917554209 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Amino acids Biomedical and Life Sciences Biomedicine DNA Erythrocytes Gene polymorphism genes Genetic analysis Genetic aspects genetic polymorphism Genetic polymorphisms Genetic vectors Haplotypes Immunology Malaria Malaysia Medical Microbiology Microbiology Natural selection parasites phenotype Phylogeny plasmid vectors Plasmodium cynomolgi Plasmodium falciparum Polymorphism Protein binding |
| title | Genetic polymorphism and clustering of the Plasmodium cynomolgi Duffy binding protein 1 region II of recent macaque isolates from Peninsular Malaysia |
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