Development of antibody to virulence factor flagellin and its evaluation in screening Ralstonia pseudosolanacearum
The bacterial wilt disease caused by Ralstonia pseudosolanacearum presents a notable economic risk to a variety of crucial crops worldwide. During preliminary isolation of this phytopathogen, several colonies of other saprophytic bacteria may be mistaken with it. So, the present study aims to addres...
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| Veröffentlicht in: | Brazilian journal of microbiology 2024-03, Vol.55 (1), p.809-821 |
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| creator | Bhatt, Shalini Raj, S. Merwyn P. Faridi, Neha Pathak, Dinesh Agarwal, Ankur Mishra, Shraddha P. |
| description | The bacterial wilt disease caused by
Ralstonia pseudosolanacearum
presents a notable economic risk to a variety of crucial crops worldwide. During preliminary isolation of this phytopathogen, several colonies of other saprophytic bacteria may be mistaken with it. So, the present study aims to address this issue by proposing the application of immunogenic proteins, particularly flagellin (FliC), to enable a rapid and early identification of bacterial wilt. In this study, a novel approach is unveiled for the early detection of
R. pseudosolanacearum
. The study exploits the immunogenic attributes of flagellin (FliC), by generating polyclonal antibodies against recombinant FliC within model organisms—rabbits and mice. The efficacy of these antibodies is meticulously assessed through discerning techniques, including DAS-ELISA and Western blot analyses, which elucidate their remarkable specificity in identifying various
R
.
pseudosolanacearum
strains. Furthermore, the introduction of antibody-coated latex agglutinating reagents offers an additional layer of confirmation, substantiating the feasibility of establishing a laboratory-based toolkit for swift screening and unambiguous identification of the bacterial wilt pathogen. This study presents a significant stride toward enhancing early diagnostic capabilities, potentially revolutionizing agricultural practices by safeguarding crop yield and quality through proactive pathogen detection and mitigation strategies.
Graphical abstract |
| doi_str_mv | 10.1007/s42770-023-01235-4 |
| format | Article |
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Ralstonia pseudosolanacearum
presents a notable economic risk to a variety of crucial crops worldwide. During preliminary isolation of this phytopathogen, several colonies of other saprophytic bacteria may be mistaken with it. So, the present study aims to address this issue by proposing the application of immunogenic proteins, particularly flagellin (FliC), to enable a rapid and early identification of bacterial wilt. In this study, a novel approach is unveiled for the early detection of
R. pseudosolanacearum
. The study exploits the immunogenic attributes of flagellin (FliC), by generating polyclonal antibodies against recombinant FliC within model organisms—rabbits and mice. The efficacy of these antibodies is meticulously assessed through discerning techniques, including DAS-ELISA and Western blot analyses, which elucidate their remarkable specificity in identifying various
R
.
pseudosolanacearum
strains. Furthermore, the introduction of antibody-coated latex agglutinating reagents offers an additional layer of confirmation, substantiating the feasibility of establishing a laboratory-based toolkit for swift screening and unambiguous identification of the bacterial wilt pathogen. This study presents a significant stride toward enhancing early diagnostic capabilities, potentially revolutionizing agricultural practices by safeguarding crop yield and quality through proactive pathogen detection and mitigation strategies.
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Ralstonia pseudosolanacearum
presents a notable economic risk to a variety of crucial crops worldwide. During preliminary isolation of this phytopathogen, several colonies of other saprophytic bacteria may be mistaken with it. So, the present study aims to address this issue by proposing the application of immunogenic proteins, particularly flagellin (FliC), to enable a rapid and early identification of bacterial wilt. In this study, a novel approach is unveiled for the early detection of
R. pseudosolanacearum
. The study exploits the immunogenic attributes of flagellin (FliC), by generating polyclonal antibodies against recombinant FliC within model organisms—rabbits and mice. The efficacy of these antibodies is meticulously assessed through discerning techniques, including DAS-ELISA and Western blot analyses, which elucidate their remarkable specificity in identifying various
R
.
pseudosolanacearum
strains. Furthermore, the introduction of antibody-coated latex agglutinating reagents offers an additional layer of confirmation, substantiating the feasibility of establishing a laboratory-based toolkit for swift screening and unambiguous identification of the bacterial wilt pathogen. This study presents a significant stride toward enhancing early diagnostic capabilities, potentially revolutionizing agricultural practices by safeguarding crop yield and quality through proactive pathogen detection and mitigation strategies.
Graphical abstract</description><subject>Agricultural practices</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Bacteria</subject><subject>Biomedical and Life Sciences</subject><subject>Crop yield</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Flagellin</subject><subject>Flagellin - genetics</subject><subject>Food Microbiology</subject><subject>Immunogenicity</subject><subject>Latex</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Mice</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Pathogens</subject><subject>Plant bacterial diseases</subject><subject>Polyclonal antibodies</subject><subject>Rabbits</subject><subject>Ralstonia</subject><subject>Ralstonia pseudosolanacearum</subject><subject>Ralstonia solanacearum</subject><subject>Reagents</subject><subject>Soil and Agricultural Microbiology - Research Paper</subject><subject>Virulence factors</subject><subject>Virulence Factors - genetics</subject><subject>Wilt</subject><issn>1517-8382</issn><issn>1678-4405</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuLFTEQhRtRnIf-ARcScOOmtfLuXomMjgoDgug6pNPV1wzdyTXpvjD_3tI7jo-Fq4TUVyd16jTNEw4vOIB9WZWwFloQsgUupG7VveaUG9u1SoG-T3fNbdvJTpw0Z7VeAwgNSjxsTuhJSqP4aVPe4AHnvF8wrSxPzKc1Dnm8YWtmh1i2GVNANvmw5sKm2e9wnmMibGRxrQwPft78GnNi9FpDQUwx7dgnP9c1p-jZvuI25ppnn3xAX7blUfNgojI-vj3Pmy-Xbz9fvG-vPr77cPH6qg003tqOwQ6DQm0HI7UQYkCvANWouJ2EnsLQg9Ej9NMQZG-g0wGgg56sm06PfS_Pm1dH3f02LDgGclj87PYlLr7cuOyj-7uS4le3ywfHoRegJSeF57cKJX_bsK5uiTXQBnzCvFUnem5o01YKQp_9g17nrSTyR5QSAoxRkihxpELJtRac7qbh4H5k6o6ZOsrU_czUKWp6-qePu5ZfIRIgj0ClUtph-f33f2S_Azd7rtg</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Bhatt, Shalini</creator><creator>Raj, S. Merwyn P.</creator><creator>Faridi, Neha</creator><creator>Pathak, Dinesh</creator><creator>Agarwal, Ankur</creator><creator>Mishra, Shraddha P.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9344-7204</orcidid></search><sort><creationdate>20240301</creationdate><title>Development of antibody to virulence factor flagellin and its evaluation in screening Ralstonia pseudosolanacearum</title><author>Bhatt, Shalini ; Raj, S. 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Ralstonia pseudosolanacearum
presents a notable economic risk to a variety of crucial crops worldwide. During preliminary isolation of this phytopathogen, several colonies of other saprophytic bacteria may be mistaken with it. So, the present study aims to address this issue by proposing the application of immunogenic proteins, particularly flagellin (FliC), to enable a rapid and early identification of bacterial wilt. In this study, a novel approach is unveiled for the early detection of
R. pseudosolanacearum
. The study exploits the immunogenic attributes of flagellin (FliC), by generating polyclonal antibodies against recombinant FliC within model organisms—rabbits and mice. The efficacy of these antibodies is meticulously assessed through discerning techniques, including DAS-ELISA and Western blot analyses, which elucidate their remarkable specificity in identifying various
R
.
pseudosolanacearum
strains. Furthermore, the introduction of antibody-coated latex agglutinating reagents offers an additional layer of confirmation, substantiating the feasibility of establishing a laboratory-based toolkit for swift screening and unambiguous identification of the bacterial wilt pathogen. This study presents a significant stride toward enhancing early diagnostic capabilities, potentially revolutionizing agricultural practices by safeguarding crop yield and quality through proactive pathogen detection and mitigation strategies.
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| ispartof | Brazilian journal of microbiology, 2024-03, Vol.55 (1), p.809-821 |
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| subjects | Agricultural practices Animals Antibodies Bacteria Biomedical and Life Sciences Crop yield Enzyme-linked immunosorbent assay Flagellin Flagellin - genetics Food Microbiology Immunogenicity Latex Life Sciences Medical Microbiology Mice Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Pathogens Plant bacterial diseases Polyclonal antibodies Rabbits Ralstonia Ralstonia pseudosolanacearum Ralstonia solanacearum Reagents Soil and Agricultural Microbiology - Research Paper Virulence factors Virulence Factors - genetics Wilt |
| title | Development of antibody to virulence factor flagellin and its evaluation in screening Ralstonia pseudosolanacearum |
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