Molecular Verification of Trichophyton in the Brazilian URM Culture Collection
Trichophyton species cause dermatophytosis in humans, with a high, worldwide frequency of reports and important public health relevance. We evaluated 61 Trichophyton strains from different sources deposited in the University Recife Mycology (URM) culture collection of the Universidade Federal de Per...
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| Veröffentlicht in: | Mycopathologia (1975) 2024-02, Vol.189 (1), p.2-2, Article 2 |
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| creator | Oliveira, Tatiana F. Valeriano, Carlos A. T. Buonafina-Paz, M. Daniela S. Souza-Motta, Cristina M. Machado, Alexandre R. Neves, Rejane P. Bezerra, Jadson D. P. Arantes, Thales D. de Hoog, Sybren Magalhães, Oliane M. C. |
| description | Trichophyton
species cause dermatophytosis in humans, with a high, worldwide frequency of reports and important public health relevance. We evaluated 61
Trichophyton
strains from different sources deposited in the University Recife Mycology (URM) culture collection of the Universidade Federal de Pernambuco, Brazil. Strains were phenotypically identified and confirmed by sequencing Internal Transcribed Spacers rDNA and partial beta-tubulin 2-exon. Additionally, we evaluated their susceptibility to terbinafine and itraconazole. Physiological analyses included urease activity and growth in casein medium. Phenotypic methods allowed the reliable identification of
T. rubrum
only, whereas, for other species, molecular methods were mandatory. All
Trichophyton
species exhibited susceptibility profiles to itraconazole (0.04–5.33 μg/mL) and terbinafine (0.17–3.33 μg/mL). Our analyses revealed a heterogeneous distribution of
T. mentagrophytes
, which does not support the current distribution within the species complex of
T. mentagrophytes
and its genotypes. |
| doi_str_mv | 10.1007/s11046-023-00811-4 |
| format | Article |
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species cause dermatophytosis in humans, with a high, worldwide frequency of reports and important public health relevance. We evaluated 61
Trichophyton
strains from different sources deposited in the University Recife Mycology (URM) culture collection of the Universidade Federal de Pernambuco, Brazil. Strains were phenotypically identified and confirmed by sequencing Internal Transcribed Spacers rDNA and partial beta-tubulin 2-exon. Additionally, we evaluated their susceptibility to terbinafine and itraconazole. Physiological analyses included urease activity and growth in casein medium. Phenotypic methods allowed the reliable identification of
T. rubrum
only, whereas, for other species, molecular methods were mandatory. All
Trichophyton
species exhibited susceptibility profiles to itraconazole (0.04–5.33 μg/mL) and terbinafine (0.17–3.33 μg/mL). Our analyses revealed a heterogeneous distribution of
T. mentagrophytes
, which does not support the current distribution within the species complex of
T. mentagrophytes
and its genotypes.</description><identifier>ISSN: 0301-486X</identifier><identifier>EISSN: 1573-0832</identifier><identifier>DOI: 10.1007/s11046-023-00811-4</identifier><identifier>PMID: 38217794</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antifungal agents ; Antifungal Agents - pharmacology ; Arthrodermataceae - genetics ; Biomedical and Life Sciences ; Brazil ; Casein ; Dermatomycosis ; Eukaryotic Microbiology ; Genotypes ; Geographical distribution ; Humans ; Itraconazole ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Sensitivity Tests ; Microbiology ; Morphology ; Original Article ; Phylogenetics ; Plant Sciences ; Public health ; Software ; Strains (organisms) ; Terbinafine ; Terbinafine - pharmacology ; Tinea ; Trichophyton ; Tubulin ; Universities ; Urease</subject><ispartof>Mycopathologia (1975), 2024-02, Vol.189 (1), p.2-2, Article 2</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-5462b251abe364c3cbcf8726cba12a52b0c26e4cc89d9c26757590416523d78e3</cites><orcidid>0000-0003-4010-5803 ; 0000-0003-4184-7366 ; 0000-0001-7275-5304 ; 0000-0002-0964-8271 ; 0000-0001-7440-3097 ; 0000-0001-5270-6302 ; 0000-0002-7782-8602 ; 0000-0002-5344-257X ; 0000-0001-5722-090X ; 0000-0002-2931-3372</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11046-023-00811-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11046-023-00811-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38217794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oliveira, Tatiana F.</creatorcontrib><creatorcontrib>Valeriano, Carlos A. T.</creatorcontrib><creatorcontrib>Buonafina-Paz, M. Daniela S.</creatorcontrib><creatorcontrib>Souza-Motta, Cristina M.</creatorcontrib><creatorcontrib>Machado, Alexandre R.</creatorcontrib><creatorcontrib>Neves, Rejane P.</creatorcontrib><creatorcontrib>Bezerra, Jadson D. P.</creatorcontrib><creatorcontrib>Arantes, Thales D.</creatorcontrib><creatorcontrib>de Hoog, Sybren</creatorcontrib><creatorcontrib>Magalhães, Oliane M. C.</creatorcontrib><title>Molecular Verification of Trichophyton in the Brazilian URM Culture Collection</title><title>Mycopathologia (1975)</title><addtitle>Mycopathologia</addtitle><addtitle>Mycopathologia</addtitle><description>Trichophyton
species cause dermatophytosis in humans, with a high, worldwide frequency of reports and important public health relevance. We evaluated 61
Trichophyton
strains from different sources deposited in the University Recife Mycology (URM) culture collection of the Universidade Federal de Pernambuco, Brazil. Strains were phenotypically identified and confirmed by sequencing Internal Transcribed Spacers rDNA and partial beta-tubulin 2-exon. Additionally, we evaluated their susceptibility to terbinafine and itraconazole. Physiological analyses included urease activity and growth in casein medium. Phenotypic methods allowed the reliable identification of
T. rubrum
only, whereas, for other species, molecular methods were mandatory. All
Trichophyton
species exhibited susceptibility profiles to itraconazole (0.04–5.33 μg/mL) and terbinafine (0.17–3.33 μg/mL). Our analyses revealed a heterogeneous distribution of
T. mentagrophytes
, which does not support the current distribution within the species complex of
T. mentagrophytes
and its genotypes.</description><subject>Antifungal agents</subject><subject>Antifungal Agents - pharmacology</subject><subject>Arthrodermataceae - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Brazil</subject><subject>Casein</subject><subject>Dermatomycosis</subject><subject>Eukaryotic Microbiology</subject><subject>Genotypes</subject><subject>Geographical distribution</subject><subject>Humans</subject><subject>Itraconazole</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Phylogenetics</subject><subject>Plant Sciences</subject><subject>Public health</subject><subject>Software</subject><subject>Strains (organisms)</subject><subject>Terbinafine</subject><subject>Terbinafine - pharmacology</subject><subject>Tinea</subject><subject>Trichophyton</subject><subject>Tubulin</subject><subject>Universities</subject><subject>Urease</subject><issn>0301-486X</issn><issn>1573-0832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LwzAch4Mobk6_gAcpePFSzXvaoxbfYCrIJt5CmqUuo2tm0h7mpzezU8GDp-SfPL8n4QfAMYLnCEJxERCClKcQkxTCDKGU7oAhYiKOGcG7YAgJjIcZfx2AgxAWEMYYEvtgQDKMhMjpEDw-uNrorlY-eTHeVlar1romcVUy8VbP3Wq-buNsm6Sdm-TKqw9bW9Uk0-eHpOjqtvMmKVwdJZvcIdirVB3M0XYdgenN9aS4S8dPt_fF5TjVBPM2ZZTjEjOkSkM41USXusoE5rpUCCuGS6gxN1TrLJ_lcSuYYDmkiDNMZiIzZATOeu_Ku_fOhFYubdCmrlVjXBckzhGN_pzRiJ7-QReu80383YYigiGBYaRwT2nvQvCmkitvl8qvJYJy07bs25axbfnVttyoT7bqrlya2U_ku94IkB4I8ap5M_737X-0n0HFiNY</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Oliveira, Tatiana F.</creator><creator>Valeriano, Carlos A. 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C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Verification of Trichophyton in the Brazilian URM Culture Collection</atitle><jtitle>Mycopathologia (1975)</jtitle><stitle>Mycopathologia</stitle><addtitle>Mycopathologia</addtitle><date>2024-02-01</date><risdate>2024</risdate><volume>189</volume><issue>1</issue><spage>2</spage><epage>2</epage><pages>2-2</pages><artnum>2</artnum><issn>0301-486X</issn><eissn>1573-0832</eissn><abstract>Trichophyton
species cause dermatophytosis in humans, with a high, worldwide frequency of reports and important public health relevance. We evaluated 61
Trichophyton
strains from different sources deposited in the University Recife Mycology (URM) culture collection of the Universidade Federal de Pernambuco, Brazil. Strains were phenotypically identified and confirmed by sequencing Internal Transcribed Spacers rDNA and partial beta-tubulin 2-exon. Additionally, we evaluated their susceptibility to terbinafine and itraconazole. Physiological analyses included urease activity and growth in casein medium. Phenotypic methods allowed the reliable identification of
T. rubrum
only, whereas, for other species, molecular methods were mandatory. All
Trichophyton
species exhibited susceptibility profiles to itraconazole (0.04–5.33 μg/mL) and terbinafine (0.17–3.33 μg/mL). Our analyses revealed a heterogeneous distribution of
T. mentagrophytes
, which does not support the current distribution within the species complex of
T. mentagrophytes
and its genotypes.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38217794</pmid><doi>10.1007/s11046-023-00811-4</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4010-5803</orcidid><orcidid>https://orcid.org/0000-0003-4184-7366</orcidid><orcidid>https://orcid.org/0000-0001-7275-5304</orcidid><orcidid>https://orcid.org/0000-0002-0964-8271</orcidid><orcidid>https://orcid.org/0000-0001-7440-3097</orcidid><orcidid>https://orcid.org/0000-0001-5270-6302</orcidid><orcidid>https://orcid.org/0000-0002-7782-8602</orcidid><orcidid>https://orcid.org/0000-0002-5344-257X</orcidid><orcidid>https://orcid.org/0000-0001-5722-090X</orcidid><orcidid>https://orcid.org/0000-0002-2931-3372</orcidid></addata></record> |
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| subjects | Antifungal agents Antifungal Agents - pharmacology Arthrodermataceae - genetics Biomedical and Life Sciences Brazil Casein Dermatomycosis Eukaryotic Microbiology Genotypes Geographical distribution Humans Itraconazole Life Sciences Medical Microbiology Microbial Ecology Microbial Sensitivity Tests Microbiology Morphology Original Article Phylogenetics Plant Sciences Public health Software Strains (organisms) Terbinafine Terbinafine - pharmacology Tinea Trichophyton Tubulin Universities Urease |
| title | Molecular Verification of Trichophyton in the Brazilian URM Culture Collection |
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