Bbhox2 is a key regulator for conidiation and virulence in Beauveria bassiana
[Display omitted] •Bbhox2 is involved in conidiation.•Bbhox2 contributes to appressorium formation.•Bbhox2 is required for the propagation of entomopathogenic fungi in the host. Beauveria bassiana, a well-known filamentous biocontrol fungus, is the main pathogen of numerous field and forest pests. T...
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Veröffentlicht in: | Journal of invertebrate pathology 2024-03, Vol.203, p.108059-108059, Article 108059 |
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container_title | Journal of invertebrate pathology |
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creator | Li, Juanjuan Zhang, Yan Jiao, Shouhao He, Lian Fan, Yanhua Han, Xuemeng Sun, Binda Zhao, Wenqi Mei, Yanlin Wei, Ning Zeng, Haiyue Jin, Dan |
description | [Display omitted]
•Bbhox2 is involved in conidiation.•Bbhox2 contributes to appressorium formation.•Bbhox2 is required for the propagation of entomopathogenic fungi in the host.
Beauveria bassiana, a well-known filamentous biocontrol fungus, is the main pathogen of numerous field and forest pests. To explore the potential factors involved in the fungal pathogenicity, Bbhox2, an important and conserved functional transcription factor containing homeodomain was carried out by functional analysis. Homologous recombination was used to disrupt the Bbhox2 gene in B.bassiana. The conidia yield of the deletant fungal strain was significantly reduced. The conidial germination was faster, and stress tolerance to Congo red and high osmotic agents were decreased compared with that in the wildtype. Additionally, ΔBbhox2 showed a dramatic reduction in virulence no matter in topical inoculations or in intra-hemolymph injections against Galleria mellonella larvae, which is likely due to the failure of appressorium formation and the defect in producing hyphal body. These results indicate that the Bbhox2 gene markedly contributes to conidiation and pathogenicity in B. bassiana. |
doi_str_mv | 10.1016/j.jip.2024.108059 |
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•Bbhox2 is involved in conidiation.•Bbhox2 contributes to appressorium formation.•Bbhox2 is required for the propagation of entomopathogenic fungi in the host.
Beauveria bassiana, a well-known filamentous biocontrol fungus, is the main pathogen of numerous field and forest pests. To explore the potential factors involved in the fungal pathogenicity, Bbhox2, an important and conserved functional transcription factor containing homeodomain was carried out by functional analysis. Homologous recombination was used to disrupt the Bbhox2 gene in B.bassiana. The conidia yield of the deletant fungal strain was significantly reduced. The conidial germination was faster, and stress tolerance to Congo red and high osmotic agents were decreased compared with that in the wildtype. Additionally, ΔBbhox2 showed a dramatic reduction in virulence no matter in topical inoculations or in intra-hemolymph injections against Galleria mellonella larvae, which is likely due to the failure of appressorium formation and the defect in producing hyphal body. These results indicate that the Bbhox2 gene markedly contributes to conidiation and pathogenicity in B. bassiana.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/j.jip.2024.108059</identifier><identifier>PMID: 38199517</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Beauveria - genetics ; Beauveria bassiana ; Conidiation ; Fungal Proteins - genetics ; Homeobox TF ; Hyphal body ; Larva - microbiology ; Moths - microbiology ; Spores, Fungal - genetics ; Virulence</subject><ispartof>Journal of invertebrate pathology, 2024-03, Vol.203, p.108059-108059, Article 108059</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c305t-5936fa240afc4f8ba9eaf5e17e36d577239bddf59d0617aa7aabba545009bddd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jip.2024.108059$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38199517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Juanjuan</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Jiao, Shouhao</creatorcontrib><creatorcontrib>He, Lian</creatorcontrib><creatorcontrib>Fan, Yanhua</creatorcontrib><creatorcontrib>Han, Xuemeng</creatorcontrib><creatorcontrib>Sun, Binda</creatorcontrib><creatorcontrib>Zhao, Wenqi</creatorcontrib><creatorcontrib>Mei, Yanlin</creatorcontrib><creatorcontrib>Wei, Ning</creatorcontrib><creatorcontrib>Zeng, Haiyue</creatorcontrib><creatorcontrib>Jin, Dan</creatorcontrib><title>Bbhox2 is a key regulator for conidiation and virulence in Beauveria bassiana</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>[Display omitted]
•Bbhox2 is involved in conidiation.•Bbhox2 contributes to appressorium formation.•Bbhox2 is required for the propagation of entomopathogenic fungi in the host.
Beauveria bassiana, a well-known filamentous biocontrol fungus, is the main pathogen of numerous field and forest pests. To explore the potential factors involved in the fungal pathogenicity, Bbhox2, an important and conserved functional transcription factor containing homeodomain was carried out by functional analysis. Homologous recombination was used to disrupt the Bbhox2 gene in B.bassiana. The conidia yield of the deletant fungal strain was significantly reduced. The conidial germination was faster, and stress tolerance to Congo red and high osmotic agents were decreased compared with that in the wildtype. Additionally, ΔBbhox2 showed a dramatic reduction in virulence no matter in topical inoculations or in intra-hemolymph injections against Galleria mellonella larvae, which is likely due to the failure of appressorium formation and the defect in producing hyphal body. These results indicate that the Bbhox2 gene markedly contributes to conidiation and pathogenicity in B. bassiana.</description><subject>Animals</subject><subject>Beauveria - genetics</subject><subject>Beauveria bassiana</subject><subject>Conidiation</subject><subject>Fungal Proteins - genetics</subject><subject>Homeobox TF</subject><subject>Hyphal body</subject><subject>Larva - microbiology</subject><subject>Moths - microbiology</subject><subject>Spores, Fungal - genetics</subject><subject>Virulence</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PwzAMhiMEYmPwA7igHLl0JGnTLuIEE1_SEBc4R27jQkrXjqSd2L8nVQdHJFu25dev5IeQc87mnPH0qppXdjMXTCRhXjCpDsiUM5VGw3BIpowJEQnG-YSceF-x0MlUHZNJvOBKSZ5NyfNt_tF-C2o9BfqJO-rwva-hax0tQxZtY42FzrYNhcbQrXV9jU2B1Db0FqHforNAc_DeQgOn5KiE2uPZvs7I2_3d6_IxWr08PC1vVlERM9lFUsVpCSJhUBZJuchBIZQSeYZxamSWiVjlxpRSGZbyDCBEnoNMJGPDwsQzcjn6blz71aPv9Nr6AusaGmx7r4XicZKolC-ClI_SwrXeOyz1xtk1uJ3mTA8UdaUDRT1Q1CPFcHOxt-_zNZq_i19sQXA9CjA8ubXotC_sgMVYh0WnTWv_sf8BHxuCvQ</recordid><startdate>202403</startdate><enddate>202403</enddate><creator>Li, Juanjuan</creator><creator>Zhang, Yan</creator><creator>Jiao, Shouhao</creator><creator>He, Lian</creator><creator>Fan, Yanhua</creator><creator>Han, Xuemeng</creator><creator>Sun, Binda</creator><creator>Zhao, Wenqi</creator><creator>Mei, Yanlin</creator><creator>Wei, Ning</creator><creator>Zeng, Haiyue</creator><creator>Jin, Dan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202403</creationdate><title>Bbhox2 is a key regulator for conidiation and virulence in Beauveria bassiana</title><author>Li, Juanjuan ; Zhang, Yan ; Jiao, Shouhao ; He, Lian ; Fan, Yanhua ; Han, Xuemeng ; Sun, Binda ; Zhao, Wenqi ; Mei, Yanlin ; Wei, Ning ; Zeng, Haiyue ; Jin, Dan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-5936fa240afc4f8ba9eaf5e17e36d577239bddf59d0617aa7aabba545009bddd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Beauveria - genetics</topic><topic>Beauveria bassiana</topic><topic>Conidiation</topic><topic>Fungal Proteins - genetics</topic><topic>Homeobox TF</topic><topic>Hyphal body</topic><topic>Larva - microbiology</topic><topic>Moths - microbiology</topic><topic>Spores, Fungal - genetics</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Juanjuan</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Jiao, Shouhao</creatorcontrib><creatorcontrib>He, Lian</creatorcontrib><creatorcontrib>Fan, Yanhua</creatorcontrib><creatorcontrib>Han, Xuemeng</creatorcontrib><creatorcontrib>Sun, Binda</creatorcontrib><creatorcontrib>Zhao, Wenqi</creatorcontrib><creatorcontrib>Mei, Yanlin</creatorcontrib><creatorcontrib>Wei, Ning</creatorcontrib><creatorcontrib>Zeng, Haiyue</creatorcontrib><creatorcontrib>Jin, Dan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Juanjuan</au><au>Zhang, Yan</au><au>Jiao, Shouhao</au><au>He, Lian</au><au>Fan, Yanhua</au><au>Han, Xuemeng</au><au>Sun, Binda</au><au>Zhao, Wenqi</au><au>Mei, Yanlin</au><au>Wei, Ning</au><au>Zeng, Haiyue</au><au>Jin, Dan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bbhox2 is a key regulator for conidiation and virulence in Beauveria bassiana</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>2024-03</date><risdate>2024</risdate><volume>203</volume><spage>108059</spage><epage>108059</epage><pages>108059-108059</pages><artnum>108059</artnum><issn>0022-2011</issn><eissn>1096-0805</eissn><abstract>[Display omitted]
•Bbhox2 is involved in conidiation.•Bbhox2 contributes to appressorium formation.•Bbhox2 is required for the propagation of entomopathogenic fungi in the host.
Beauveria bassiana, a well-known filamentous biocontrol fungus, is the main pathogen of numerous field and forest pests. To explore the potential factors involved in the fungal pathogenicity, Bbhox2, an important and conserved functional transcription factor containing homeodomain was carried out by functional analysis. Homologous recombination was used to disrupt the Bbhox2 gene in B.bassiana. The conidia yield of the deletant fungal strain was significantly reduced. The conidial germination was faster, and stress tolerance to Congo red and high osmotic agents were decreased compared with that in the wildtype. Additionally, ΔBbhox2 showed a dramatic reduction in virulence no matter in topical inoculations or in intra-hemolymph injections against Galleria mellonella larvae, which is likely due to the failure of appressorium formation and the defect in producing hyphal body. These results indicate that the Bbhox2 gene markedly contributes to conidiation and pathogenicity in B. bassiana.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38199517</pmid><doi>10.1016/j.jip.2024.108059</doi><tpages>1</tpages></addata></record> |
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subjects | Animals Beauveria - genetics Beauveria bassiana Conidiation Fungal Proteins - genetics Homeobox TF Hyphal body Larva - microbiology Moths - microbiology Spores, Fungal - genetics Virulence |
title | Bbhox2 is a key regulator for conidiation and virulence in Beauveria bassiana |
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