First serological diagnosis of Mycobacterium avium subsp. paratuberculosis infection in sheep in the state of Pernambuco, Brazil
This study aimed to diagnose Mycobacterium avium subsp. paratuberculosis (MAP) infections in sheep in the state of Pernambuco, Brazil. A total of 276 blood samples were analyzed using the enzyme-linked immunosorbent assay IDEXX Paratuberculosis Screening kit, and 261 fecal samples were submitted for...
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| Veröffentlicht in: | Veterinary research communications 2024-04, Vol.48 (2), p.1293-1299 |
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| creator | de Noronha Xavier, Amanda de Sá, Luenda Menezes Novaes de Nazaré Santos Ferreira, Maria de Oliveira, Pollyanne Raysa Fernandes de Moraes Peixoto, Rodolfo Mota, Rinaldo Aparecido Junior, José Wilton Pinheiro |
| description | This study aimed to diagnose
Mycobacterium avium
subsp.
paratuberculosis
(MAP) infections in sheep in the state of Pernambuco, Brazil. A total of 276 blood samples were analyzed using the enzyme-linked immunosorbent assay IDEXX Paratuberculosis Screening kit, and 261 fecal samples were submitted for bacterial culture and polymerase chain reaction tests. An animal-level sero-frequency of 0.72% (
n =
2/276) and a farm-level sero-frequency of 20% (
n =
2/10) were found. All fecal sample cultures were negative, and molecular analyses were also negative. To the best of our knowledge, this is the first study of MAP infection in sheep in the state of Pernambuco and one of the pioneers in the country. It is an asymptomatic disease that is difficult to diagnose in this species because the susceptibility of sheep to the organism is lower than that of other ruminant species. However, the sero-frequency found reveals that there is MAP exposure in sheep flocks in the region. In addition, serological monitoring can contribute to the observation of the organism’s behavior in herds. Our results support the potential risk of MAP infection in sheep in the state of Pernambuco, Brazil. |
| doi_str_mv | 10.1007/s11259-024-10300-8 |
| format | Article |
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Mycobacterium avium
subsp.
paratuberculosis
(MAP) infections in sheep in the state of Pernambuco, Brazil. A total of 276 blood samples were analyzed using the enzyme-linked immunosorbent assay IDEXX Paratuberculosis Screening kit, and 261 fecal samples were submitted for bacterial culture and polymerase chain reaction tests. An animal-level sero-frequency of 0.72% (
n =
2/276) and a farm-level sero-frequency of 20% (
n =
2/10) were found. All fecal sample cultures were negative, and molecular analyses were also negative. To the best of our knowledge, this is the first study of MAP infection in sheep in the state of Pernambuco and one of the pioneers in the country. It is an asymptomatic disease that is difficult to diagnose in this species because the susceptibility of sheep to the organism is lower than that of other ruminant species. However, the sero-frequency found reveals that there is MAP exposure in sheep flocks in the region. In addition, serological monitoring can contribute to the observation of the organism’s behavior in herds. Our results support the potential risk of MAP infection in sheep in the state of Pernambuco, Brazil.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-024-10300-8</identifier><identifier>PMID: 38206561</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Brief Report ; Enzyme-linked immunosorbent assay ; Feces ; Infections ; Life Sciences ; Mycobacterium avium ; Paratuberculosis ; Serology ; Sheep ; Veterinary Medicine/Veterinary Science ; Zoology</subject><ispartof>Veterinary research communications, 2024-04, Vol.48 (2), p.1293-1299</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-6754c139216da9a052f5d8354c986a5bb81207e8629f78895009a9e50add364b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11259-024-10300-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11259-024-10300-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38206561$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Noronha Xavier, Amanda</creatorcontrib><creatorcontrib>de Sá, Luenda Menezes Novaes</creatorcontrib><creatorcontrib>de Nazaré Santos Ferreira, Maria</creatorcontrib><creatorcontrib>de Oliveira, Pollyanne Raysa Fernandes</creatorcontrib><creatorcontrib>de Moraes Peixoto, Rodolfo</creatorcontrib><creatorcontrib>Mota, Rinaldo Aparecido</creatorcontrib><creatorcontrib>Junior, José Wilton Pinheiro</creatorcontrib><title>First serological diagnosis of Mycobacterium avium subsp. paratuberculosis infection in sheep in the state of Pernambuco, Brazil</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>This study aimed to diagnose
Mycobacterium avium
subsp.
paratuberculosis
(MAP) infections in sheep in the state of Pernambuco, Brazil. A total of 276 blood samples were analyzed using the enzyme-linked immunosorbent assay IDEXX Paratuberculosis Screening kit, and 261 fecal samples were submitted for bacterial culture and polymerase chain reaction tests. An animal-level sero-frequency of 0.72% (
n =
2/276) and a farm-level sero-frequency of 20% (
n =
2/10) were found. All fecal sample cultures were negative, and molecular analyses were also negative. To the best of our knowledge, this is the first study of MAP infection in sheep in the state of Pernambuco and one of the pioneers in the country. It is an asymptomatic disease that is difficult to diagnose in this species because the susceptibility of sheep to the organism is lower than that of other ruminant species. However, the sero-frequency found reveals that there is MAP exposure in sheep flocks in the region. In addition, serological monitoring can contribute to the observation of the organism’s behavior in herds. Our results support the potential risk of MAP infection in sheep in the state of Pernambuco, Brazil.</description><subject>Biomedical and Life Sciences</subject><subject>Brief Report</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Feces</subject><subject>Infections</subject><subject>Life Sciences</subject><subject>Mycobacterium avium</subject><subject>Paratuberculosis</subject><subject>Serology</subject><subject>Sheep</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Zoology</subject><issn>0165-7380</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kcFuFSEUhomxsdfqC7gwJG66cOoBBoZZatOqSRtd6JowDHNLMzOMHMakrnx0md6qiQs3cAIf_znkI-QFgzMG0LxBxrhsK-B1xUAAVPoR2THZiKqpa_WY7IApWTVCwzF5ingLAK0G8YQcC81BScV25OdlSJgp-hTHuA_OjrQPdj9HDEjjQK_vXOysyz6FdaL2-7bi2uFyRhebbF47n9w63uNhHrzLIc6lonjj_bIV-cZTzDb7Le6zT7OdutXF1_Rdsj_C-IwcDXZE__xhPyFfLy--nH-orj69_3j-9qpygqtcqUbWjomWM9Xb1oLkg-y1KIetVlZ2nWYcGq8Vb4dG61aWv9rWS7B9L1TdiRNyeshdUvy2esxmCuj8ONrZxxUNb5moa6FrXdBX_6C3cS1zj2gEiDJDaSkKxQ-USxEx-cEsKUw23RkGZvNjDn5M8WPu_Zgt-uVD9NpNvv_z5LeQAogDgOVq3vv0t_d_Yn8BaLKbYQ</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>de Noronha Xavier, Amanda</creator><creator>de Sá, Luenda Menezes Novaes</creator><creator>de Nazaré Santos Ferreira, Maria</creator><creator>de Oliveira, Pollyanne Raysa Fernandes</creator><creator>de Moraes Peixoto, Rodolfo</creator><creator>Mota, Rinaldo Aparecido</creator><creator>Junior, José Wilton Pinheiro</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20240401</creationdate><title>First serological diagnosis of Mycobacterium avium subsp. paratuberculosis infection in sheep in the state of Pernambuco, Brazil</title><author>de Noronha Xavier, Amanda ; de Sá, Luenda Menezes Novaes ; de Nazaré Santos Ferreira, Maria ; de Oliveira, Pollyanne Raysa Fernandes ; de Moraes Peixoto, Rodolfo ; Mota, Rinaldo Aparecido ; Junior, José Wilton Pinheiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-6754c139216da9a052f5d8354c986a5bb81207e8629f78895009a9e50add364b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biomedical and Life Sciences</topic><topic>Brief Report</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Feces</topic><topic>Infections</topic><topic>Life Sciences</topic><topic>Mycobacterium avium</topic><topic>Paratuberculosis</topic><topic>Serology</topic><topic>Sheep</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Noronha Xavier, Amanda</creatorcontrib><creatorcontrib>de Sá, Luenda Menezes Novaes</creatorcontrib><creatorcontrib>de Nazaré Santos Ferreira, Maria</creatorcontrib><creatorcontrib>de Oliveira, Pollyanne Raysa Fernandes</creatorcontrib><creatorcontrib>de Moraes Peixoto, Rodolfo</creatorcontrib><creatorcontrib>Mota, Rinaldo Aparecido</creatorcontrib><creatorcontrib>Junior, José Wilton Pinheiro</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Noronha Xavier, Amanda</au><au>de Sá, Luenda Menezes Novaes</au><au>de Nazaré Santos Ferreira, Maria</au><au>de Oliveira, Pollyanne Raysa Fernandes</au><au>de Moraes Peixoto, Rodolfo</au><au>Mota, Rinaldo Aparecido</au><au>Junior, José Wilton Pinheiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First serological diagnosis of Mycobacterium avium subsp. paratuberculosis infection in sheep in the state of Pernambuco, Brazil</atitle><jtitle>Veterinary research communications</jtitle><stitle>Vet Res Commun</stitle><addtitle>Vet Res Commun</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>48</volume><issue>2</issue><spage>1293</spage><epage>1299</epage><pages>1293-1299</pages><issn>0165-7380</issn><eissn>1573-7446</eissn><abstract>This study aimed to diagnose
Mycobacterium avium
subsp.
paratuberculosis
(MAP) infections in sheep in the state of Pernambuco, Brazil. A total of 276 blood samples were analyzed using the enzyme-linked immunosorbent assay IDEXX Paratuberculosis Screening kit, and 261 fecal samples were submitted for bacterial culture and polymerase chain reaction tests. An animal-level sero-frequency of 0.72% (
n =
2/276) and a farm-level sero-frequency of 20% (
n =
2/10) were found. All fecal sample cultures were negative, and molecular analyses were also negative. To the best of our knowledge, this is the first study of MAP infection in sheep in the state of Pernambuco and one of the pioneers in the country. It is an asymptomatic disease that is difficult to diagnose in this species because the susceptibility of sheep to the organism is lower than that of other ruminant species. However, the sero-frequency found reveals that there is MAP exposure in sheep flocks in the region. In addition, serological monitoring can contribute to the observation of the organism’s behavior in herds. Our results support the potential risk of MAP infection in sheep in the state of Pernambuco, Brazil.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38206561</pmid><doi>10.1007/s11259-024-10300-8</doi><tpages>7</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Brief Report Enzyme-linked immunosorbent assay Feces Infections Life Sciences Mycobacterium avium Paratuberculosis Serology Sheep Veterinary Medicine/Veterinary Science Zoology |
| title | First serological diagnosis of Mycobacterium avium subsp. paratuberculosis infection in sheep in the state of Pernambuco, Brazil |
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