SCARPET: site-specific quantification of methylated and nonmethylated adenosines reveals m6A stoichiometry

m6A has different stoichiometry at different positions in different mRNAs. However, the exact stoichiometry of m6A is difficult to measure. Here, we describe SCARPET (site-specific cleavage and radioactive-labeling followed by purification, exonuclease digestion, and thin-layer chromatography), a si...

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Veröffentlicht in:RNA (Cambridge) 2024-03, Vol.30 (3), p.308
Hauptverfasser: Mirza, Aashiq H, Bram, Yaron, Schwartz, Robert E, Jaffrey, Samie R
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Sprache:eng
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