The Bronchodilator Potential of Astragalus sarcocolla: An in vitro Experiment
To evaluate the bronchodilatory mechanism of Astragalus sarcocolla (ASE) extract on tracheal smooth muscles of rabbits. In-vitro experimental study. Place and Duration of the Study: The animal house of CMH Lahore Medical College, Lahore, and Institute of Dentistry, NUMS, from October 2022 to May 202...
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| Veröffentlicht in: | Journal of the College of Physicians and Surgeons--Pakistan 2024-01, Vol.34 (1), p.58-62 |
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| creator | Siddiqui, Waqar Ahmed Qayyum, Muniza Qureshi, Aamina Quddus Khalid, Momina Zaffar, Sehrish Bilal, Rabiea |
| description | To evaluate the bronchodilatory mechanism of Astragalus sarcocolla (ASE) extract on tracheal smooth muscles of rabbits.
In-vitro experimental study. Place and Duration of the Study: The animal house of CMH Lahore Medical College, Lahore, and Institute of Dentistry, NUMS, from October 2022 to May 2023.
Six rabbits were randomly divided into four groups. After euthanising the rabbit, the trachea was carefully dissected out and stabilised in Kreb's Henseleit solution for 30 minutes and then, stimulated by acetylcholine (Ach) 1µm, under mimicked physiological conditions. Group I served as the control group with tracheal smooth muscles stabilised with 1g tension. In Group II (positive control), tracheal smooth muscles were stimulated by potassium chloride (KCl) (80 mM and 25 mM, respectively) to get maximum tracheal smooth muscle contractions. Later, the tissue was exposed to theophylline with three molar concentrations 0.2, 0.4, 0.6, and 0.8 mM, and cumulative dose response curves were formed. In Group III (ASE group), tracheal smooth muscles were stimulated by KCl (80 mM and 25 mM) and was exposed to increasing concentration of ASE. In group IV, tissue was stimulated by KCl (25 mM) and glibenclamide (3 µM), later exposed to increasing concentration of ASE to confirm the bronchodilatory mechanism. The change in isometric contraction of the tissue was recorded using the force displacement transducer connected to a PowerLab data acquisition system. Concentration response curves were drawn, and median effective concentrations (EC50 values) and percentage inhibition were calculated. Non-linear regression was applied for the analysis of the concentration-response curves.
ASE inhibited the KCl-induced low potassium (25 mM) contractions (EC50 = 0.38 mg/ml, 95% CI: 0.04 - 0.38, n = 6). It only partially inhibited the high potassium-induced contractions in tracheal smooth muscles. Pretreatment with glibenclamide showed a rightward shift of the dose-response curve. Theophylline and ASE significantly reduced the low K+ induced smooth muscle contractions in comparison to the control group (p |
| doi_str_mv | 10.29271/jcpsp.2024.01.58 |
| format | Article |
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In-vitro experimental study. Place and Duration of the Study: The animal house of CMH Lahore Medical College, Lahore, and Institute of Dentistry, NUMS, from October 2022 to May 2023.
Six rabbits were randomly divided into four groups. After euthanising the rabbit, the trachea was carefully dissected out and stabilised in Kreb's Henseleit solution for 30 minutes and then, stimulated by acetylcholine (Ach) 1µm, under mimicked physiological conditions. Group I served as the control group with tracheal smooth muscles stabilised with 1g tension. In Group II (positive control), tracheal smooth muscles were stimulated by potassium chloride (KCl) (80 mM and 25 mM, respectively) to get maximum tracheal smooth muscle contractions. Later, the tissue was exposed to theophylline with three molar concentrations 0.2, 0.4, 0.6, and 0.8 mM, and cumulative dose response curves were formed. In Group III (ASE group), tracheal smooth muscles were stimulated by KCl (80 mM and 25 mM) and was exposed to increasing concentration of ASE. In group IV, tissue was stimulated by KCl (25 mM) and glibenclamide (3 µM), later exposed to increasing concentration of ASE to confirm the bronchodilatory mechanism. The change in isometric contraction of the tissue was recorded using the force displacement transducer connected to a PowerLab data acquisition system. Concentration response curves were drawn, and median effective concentrations (EC50 values) and percentage inhibition were calculated. Non-linear regression was applied for the analysis of the concentration-response curves.
ASE inhibited the KCl-induced low potassium (25 mM) contractions (EC50 = 0.38 mg/ml, 95% CI: 0.04 - 0.38, n = 6). It only partially inhibited the high potassium-induced contractions in tracheal smooth muscles. Pretreatment with glibenclamide showed a rightward shift of the dose-response curve. Theophylline and ASE significantly reduced the low K+ induced smooth muscle contractions in comparison to the control group (p <0.001, each).
Astragalus sarcocolla extract produced bronchodilator effects through the activation of ATP sensitive potassium channels in isolated rabbit trachea.
Astragalus sarcocolla, Bronchodilators, ATP-sensitive potassium channels, Effective concentration 50, Concentration response curves.</description><identifier>ISSN: 1022-386X</identifier><identifier>EISSN: 1681-7168</identifier><identifier>DOI: 10.29271/jcpsp.2024.01.58</identifier><identifier>PMID: 38185962</identifier><language>eng</language><publisher>Pakistan: College of Physicians and Surgeons Pakistan</publisher><subject>Asthma ; Astragalus (Plants) ; Care and treatment ; Chemical properties ; Health aspects ; Medicine, Botanic ; Medicine, Herbal ; Physiological aspects</subject><ispartof>Journal of the College of Physicians and Surgeons--Pakistan, 2024-01, Vol.34 (1), p.58-62</ispartof><rights>COPYRIGHT 2024 College of Physicians and Surgeons Pakistan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38185962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Siddiqui, Waqar Ahmed</creatorcontrib><creatorcontrib>Qayyum, Muniza</creatorcontrib><creatorcontrib>Qureshi, Aamina Quddus</creatorcontrib><creatorcontrib>Khalid, Momina</creatorcontrib><creatorcontrib>Zaffar, Sehrish</creatorcontrib><creatorcontrib>Bilal, Rabiea</creatorcontrib><title>The Bronchodilator Potential of Astragalus sarcocolla: An in vitro Experiment</title><title>Journal of the College of Physicians and Surgeons--Pakistan</title><addtitle>J Coll Physicians Surg Pak</addtitle><description>To evaluate the bronchodilatory mechanism of Astragalus sarcocolla (ASE) extract on tracheal smooth muscles of rabbits.
In-vitro experimental study. Place and Duration of the Study: The animal house of CMH Lahore Medical College, Lahore, and Institute of Dentistry, NUMS, from October 2022 to May 2023.
Six rabbits were randomly divided into four groups. After euthanising the rabbit, the trachea was carefully dissected out and stabilised in Kreb's Henseleit solution for 30 minutes and then, stimulated by acetylcholine (Ach) 1µm, under mimicked physiological conditions. Group I served as the control group with tracheal smooth muscles stabilised with 1g tension. In Group II (positive control), tracheal smooth muscles were stimulated by potassium chloride (KCl) (80 mM and 25 mM, respectively) to get maximum tracheal smooth muscle contractions. Later, the tissue was exposed to theophylline with three molar concentrations 0.2, 0.4, 0.6, and 0.8 mM, and cumulative dose response curves were formed. In Group III (ASE group), tracheal smooth muscles were stimulated by KCl (80 mM and 25 mM) and was exposed to increasing concentration of ASE. In group IV, tissue was stimulated by KCl (25 mM) and glibenclamide (3 µM), later exposed to increasing concentration of ASE to confirm the bronchodilatory mechanism. The change in isometric contraction of the tissue was recorded using the force displacement transducer connected to a PowerLab data acquisition system. Concentration response curves were drawn, and median effective concentrations (EC50 values) and percentage inhibition were calculated. Non-linear regression was applied for the analysis of the concentration-response curves.
ASE inhibited the KCl-induced low potassium (25 mM) contractions (EC50 = 0.38 mg/ml, 95% CI: 0.04 - 0.38, n = 6). It only partially inhibited the high potassium-induced contractions in tracheal smooth muscles. Pretreatment with glibenclamide showed a rightward shift of the dose-response curve. Theophylline and ASE significantly reduced the low K+ induced smooth muscle contractions in comparison to the control group (p <0.001, each).
Astragalus sarcocolla extract produced bronchodilator effects through the activation of ATP sensitive potassium channels in isolated rabbit trachea.
Astragalus sarcocolla, Bronchodilators, ATP-sensitive potassium channels, Effective concentration 50, Concentration response curves.</description><subject>Asthma</subject><subject>Astragalus (Plants)</subject><subject>Care and treatment</subject><subject>Chemical properties</subject><subject>Health aspects</subject><subject>Medicine, Botanic</subject><subject>Medicine, Herbal</subject><subject>Physiological aspects</subject><issn>1022-386X</issn><issn>1681-7168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNptkUtr3DAUhUVpyEweP6CbIuimGzt62brqbhLygoRkkUJ3QiPJjQaP5Uqekvz7aDJpIRAEV-Lyncs9Ogh9oaRmikl6srJjHmtGmKgJrRv4hOa0BVrJUj-XN2Gs4tD-mqGDnFeE8IYC7KMZBwqNatkc3T48enya4mAfowu9mWLC93HywxRMj2OHF3lK5rfpNxlnk2y0se_ND7wYcBjw3zCliM-fRp_CumiO0F5n-uyP3-5D9PPi_OHsqrq5u7w-W9xUlrd8qppWOgFOdo4tG-VBtAqMIF51yjRLZ7l1zikiloIpAO4AJGeeilZyU4wbfoi-7-aOKf7Z-DzpdcjWl80GHzdZM0UpiEYyXtBvO7R48DoMXSx-7BbXCwkF4FSKQtUfUOU4vw42Dr4Lpf9OQHcCm2LOyXd6LF9g0rOmRL-Go1_D0dtwNKG6gaL5-rb1Zrn27r_iXxr8Bb7Sigs</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Siddiqui, Waqar Ahmed</creator><creator>Qayyum, Muniza</creator><creator>Qureshi, Aamina Quddus</creator><creator>Khalid, Momina</creator><creator>Zaffar, Sehrish</creator><creator>Bilal, Rabiea</creator><general>College of Physicians and Surgeons Pakistan</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202401</creationdate><title>The Bronchodilator Potential of Astragalus sarcocolla: An in vitro Experiment</title><author>Siddiqui, Waqar Ahmed ; Qayyum, Muniza ; Qureshi, Aamina Quddus ; Khalid, Momina ; Zaffar, Sehrish ; Bilal, Rabiea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-567d48d7fd2b59e84698a40e9f9a5bdc3cddd904b429883d88732e14673a292a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Asthma</topic><topic>Astragalus (Plants)</topic><topic>Care and treatment</topic><topic>Chemical properties</topic><topic>Health aspects</topic><topic>Medicine, Botanic</topic><topic>Medicine, Herbal</topic><topic>Physiological aspects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siddiqui, Waqar Ahmed</creatorcontrib><creatorcontrib>Qayyum, Muniza</creatorcontrib><creatorcontrib>Qureshi, Aamina Quddus</creatorcontrib><creatorcontrib>Khalid, Momina</creatorcontrib><creatorcontrib>Zaffar, Sehrish</creatorcontrib><creatorcontrib>Bilal, Rabiea</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the College of Physicians and Surgeons--Pakistan</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siddiqui, Waqar Ahmed</au><au>Qayyum, Muniza</au><au>Qureshi, Aamina Quddus</au><au>Khalid, Momina</au><au>Zaffar, Sehrish</au><au>Bilal, Rabiea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Bronchodilator Potential of Astragalus sarcocolla: An in vitro Experiment</atitle><jtitle>Journal of the College of Physicians and Surgeons--Pakistan</jtitle><addtitle>J Coll Physicians Surg Pak</addtitle><date>2024-01</date><risdate>2024</risdate><volume>34</volume><issue>1</issue><spage>58</spage><epage>62</epage><pages>58-62</pages><issn>1022-386X</issn><eissn>1681-7168</eissn><abstract>To evaluate the bronchodilatory mechanism of Astragalus sarcocolla (ASE) extract on tracheal smooth muscles of rabbits.
In-vitro experimental study. Place and Duration of the Study: The animal house of CMH Lahore Medical College, Lahore, and Institute of Dentistry, NUMS, from October 2022 to May 2023.
Six rabbits were randomly divided into four groups. After euthanising the rabbit, the trachea was carefully dissected out and stabilised in Kreb's Henseleit solution for 30 minutes and then, stimulated by acetylcholine (Ach) 1µm, under mimicked physiological conditions. Group I served as the control group with tracheal smooth muscles stabilised with 1g tension. In Group II (positive control), tracheal smooth muscles were stimulated by potassium chloride (KCl) (80 mM and 25 mM, respectively) to get maximum tracheal smooth muscle contractions. Later, the tissue was exposed to theophylline with three molar concentrations 0.2, 0.4, 0.6, and 0.8 mM, and cumulative dose response curves were formed. In Group III (ASE group), tracheal smooth muscles were stimulated by KCl (80 mM and 25 mM) and was exposed to increasing concentration of ASE. In group IV, tissue was stimulated by KCl (25 mM) and glibenclamide (3 µM), later exposed to increasing concentration of ASE to confirm the bronchodilatory mechanism. The change in isometric contraction of the tissue was recorded using the force displacement transducer connected to a PowerLab data acquisition system. Concentration response curves were drawn, and median effective concentrations (EC50 values) and percentage inhibition were calculated. Non-linear regression was applied for the analysis of the concentration-response curves.
ASE inhibited the KCl-induced low potassium (25 mM) contractions (EC50 = 0.38 mg/ml, 95% CI: 0.04 - 0.38, n = 6). It only partially inhibited the high potassium-induced contractions in tracheal smooth muscles. Pretreatment with glibenclamide showed a rightward shift of the dose-response curve. Theophylline and ASE significantly reduced the low K+ induced smooth muscle contractions in comparison to the control group (p <0.001, each).
Astragalus sarcocolla extract produced bronchodilator effects through the activation of ATP sensitive potassium channels in isolated rabbit trachea.
Astragalus sarcocolla, Bronchodilators, ATP-sensitive potassium channels, Effective concentration 50, Concentration response curves.</abstract><cop>Pakistan</cop><pub>College of Physicians and Surgeons Pakistan</pub><pmid>38185962</pmid><doi>10.29271/jcpsp.2024.01.58</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Asthma Astragalus (Plants) Care and treatment Chemical properties Health aspects Medicine, Botanic Medicine, Herbal Physiological aspects |
| title | The Bronchodilator Potential of Astragalus sarcocolla: An in vitro Experiment |
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