Expression of accessory genes in Salmonella requires the presence of the Gre factors

Genomic studies with Salmonella enterica serovar Typhimurium reveal a crucial role of horizontal gene transfer (HGT) in the acquisition of accessory cellular functions involved in host-interaction. Many virulence genes are located in genomic islands, plasmids and prophages. GreA and GreB proteins, G...

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Veröffentlicht in:Genomics (San Diego, Calif.) Calif.), 2024-01, Vol.116 (1), p.110777, Article 110777
Hauptverfasser: Gaviria-Cantin, Tania, Fernández-Coll, Llorenç, Vargas, Andrés Felipe, Jiménez, Carlos Jonay, Madrid, Cristina, Balsalobre, Carlos
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container_issue 1
container_start_page 110777
container_title Genomics (San Diego, Calif.)
container_volume 116
creator Gaviria-Cantin, Tania
Fernández-Coll, Llorenç
Vargas, Andrés Felipe
Jiménez, Carlos Jonay
Madrid, Cristina
Balsalobre, Carlos
description Genomic studies with Salmonella enterica serovar Typhimurium reveal a crucial role of horizontal gene transfer (HGT) in the acquisition of accessory cellular functions involved in host-interaction. Many virulence genes are located in genomic islands, plasmids and prophages. GreA and GreB proteins, Gre factors, interact transiently with the RNA polymerase alleviating backtracked complexes during transcription elongation. The overall effect of Gre factors depletion in Salmonella expression profile was studied. Both proteins are functionally redundant since only when both Gre factors were depleted a major effect in gene expression was detected. Remarkably, the accessory gene pool is particularly sensitive to the lack of Gre factors, with 18.6% of accessory genes stimulated by the Gre factors versus 4.4% of core genome genes. Gre factors involvement is particularly relevant for the expression of genes located in genomic islands. Our data reveal that Gre factors are required for the expression of accessory genes. •Gre factors are required for transcriptional expression of accessory genes in Salmonella.•Expression of SPI-2 and SPI-2 coregulated genes, involved in intracellular survival, are highly dependent on the Gre factors.•GreA and GreB are functionally redundant in Salmonella.
doi_str_mv 10.1016/j.ygeno.2023.110777
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subjects Accessory genes
Gene expression
Gre factors
Salmonella
Transcription elongation
Virulence
title Expression of accessory genes in Salmonella requires the presence of the Gre factors
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