Expression of accessory genes in Salmonella requires the presence of the Gre factors
Genomic studies with Salmonella enterica serovar Typhimurium reveal a crucial role of horizontal gene transfer (HGT) in the acquisition of accessory cellular functions involved in host-interaction. Many virulence genes are located in genomic islands, plasmids and prophages. GreA and GreB proteins, G...
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Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2024-01, Vol.116 (1), p.110777, Article 110777 |
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creator | Gaviria-Cantin, Tania Fernández-Coll, Llorenç Vargas, Andrés Felipe Jiménez, Carlos Jonay Madrid, Cristina Balsalobre, Carlos |
description | Genomic studies with Salmonella enterica serovar Typhimurium reveal a crucial role of horizontal gene transfer (HGT) in the acquisition of accessory cellular functions involved in host-interaction. Many virulence genes are located in genomic islands, plasmids and prophages. GreA and GreB proteins, Gre factors, interact transiently with the RNA polymerase alleviating backtracked complexes during transcription elongation. The overall effect of Gre factors depletion in Salmonella expression profile was studied. Both proteins are functionally redundant since only when both Gre factors were depleted a major effect in gene expression was detected. Remarkably, the accessory gene pool is particularly sensitive to the lack of Gre factors, with 18.6% of accessory genes stimulated by the Gre factors versus 4.4% of core genome genes. Gre factors involvement is particularly relevant for the expression of genes located in genomic islands. Our data reveal that Gre factors are required for the expression of accessory genes.
•Gre factors are required for transcriptional expression of accessory genes in Salmonella.•Expression of SPI-2 and SPI-2 coregulated genes, involved in intracellular survival, are highly dependent on the Gre factors.•GreA and GreB are functionally redundant in Salmonella. |
doi_str_mv | 10.1016/j.ygeno.2023.110777 |
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•Gre factors are required for transcriptional expression of accessory genes in Salmonella.•Expression of SPI-2 and SPI-2 coregulated genes, involved in intracellular survival, are highly dependent on the Gre factors.•GreA and GreB are functionally redundant in Salmonella.</description><identifier>ISSN: 0888-7543</identifier><identifier>ISSN: 1089-8646</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/j.ygeno.2023.110777</identifier><identifier>PMID: 38163572</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Accessory genes ; Gene expression ; Gre factors ; Salmonella ; Transcription elongation ; Virulence</subject><ispartof>Genomics (San Diego, Calif.), 2024-01, Vol.116 (1), p.110777, Article 110777</ispartof><rights>2023 The Authors</rights><rights>Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c354t-84d6aed4d0de31de0ec1cb6ac4283de3bb529df5fbad071ee59ea9e642e526aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754323002215$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,860,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38163572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaviria-Cantin, Tania</creatorcontrib><creatorcontrib>Fernández-Coll, Llorenç</creatorcontrib><creatorcontrib>Vargas, Andrés Felipe</creatorcontrib><creatorcontrib>Jiménez, Carlos Jonay</creatorcontrib><creatorcontrib>Madrid, Cristina</creatorcontrib><creatorcontrib>Balsalobre, Carlos</creatorcontrib><title>Expression of accessory genes in Salmonella requires the presence of the Gre factors</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Genomic studies with Salmonella enterica serovar Typhimurium reveal a crucial role of horizontal gene transfer (HGT) in the acquisition of accessory cellular functions involved in host-interaction. Many virulence genes are located in genomic islands, plasmids and prophages. GreA and GreB proteins, Gre factors, interact transiently with the RNA polymerase alleviating backtracked complexes during transcription elongation. The overall effect of Gre factors depletion in Salmonella expression profile was studied. Both proteins are functionally redundant since only when both Gre factors were depleted a major effect in gene expression was detected. Remarkably, the accessory gene pool is particularly sensitive to the lack of Gre factors, with 18.6% of accessory genes stimulated by the Gre factors versus 4.4% of core genome genes. Gre factors involvement is particularly relevant for the expression of genes located in genomic islands. Our data reveal that Gre factors are required for the expression of accessory genes.
•Gre factors are required for transcriptional expression of accessory genes in Salmonella.•Expression of SPI-2 and SPI-2 coregulated genes, involved in intracellular survival, are highly dependent on the Gre factors.•GreA and GreB are functionally redundant in Salmonella.</description><subject>Accessory genes</subject><subject>Gene expression</subject><subject>Gre factors</subject><subject>Salmonella</subject><subject>Transcription elongation</subject><subject>Virulence</subject><issn>0888-7543</issn><issn>1089-8646</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhi0EoqXwC5CQR5YEf-TDGRhQVQpSJQbKbDn2BVKlcWsniP57HFIYmU53et47-0HompKYEprdbeLDO7Q2ZoTxmFKS5_kJmlIiikhkSXaKpkQIEeVpwifowvsNIaTggp2jCRc042nOpmi9-No58L62LbYVVlqHxroDDqvB47rFr6rZ2haaRmEH-74ONO4-AA8xaDUMsaFfOsCV0p11_hKdVarxcHWsM_T2uFjPn6LVy_J5_rCKNE-TLhKJyRSYxBADnBogoKkuM6UTJngYlWXKClOlVakMySlAWoAqIEsYpCxTis_Q7bh35-y-B9_Jbe318NQWbO8lK0hBRCJSFlA-otpZ7x1UcufqrXIHSYkcdMqN_NEpB51y1BlSN8cDfbkF85f59ReA-xGA8M3PGpz0uh6smOBJd9LY-t8D36epiRY</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Gaviria-Cantin, Tania</creator><creator>Fernández-Coll, Llorenç</creator><creator>Vargas, Andrés Felipe</creator><creator>Jiménez, Carlos Jonay</creator><creator>Madrid, Cristina</creator><creator>Balsalobre, Carlos</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240101</creationdate><title>Expression of accessory genes in Salmonella requires the presence of the Gre factors</title><author>Gaviria-Cantin, Tania ; Fernández-Coll, Llorenç ; Vargas, Andrés Felipe ; Jiménez, Carlos Jonay ; Madrid, Cristina ; Balsalobre, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-84d6aed4d0de31de0ec1cb6ac4283de3bb529df5fbad071ee59ea9e642e526aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Accessory genes</topic><topic>Gene expression</topic><topic>Gre factors</topic><topic>Salmonella</topic><topic>Transcription elongation</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaviria-Cantin, Tania</creatorcontrib><creatorcontrib>Fernández-Coll, Llorenç</creatorcontrib><creatorcontrib>Vargas, Andrés Felipe</creatorcontrib><creatorcontrib>Jiménez, Carlos Jonay</creatorcontrib><creatorcontrib>Madrid, Cristina</creatorcontrib><creatorcontrib>Balsalobre, Carlos</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaviria-Cantin, Tania</au><au>Fernández-Coll, Llorenç</au><au>Vargas, Andrés Felipe</au><au>Jiménez, Carlos Jonay</au><au>Madrid, Cristina</au><au>Balsalobre, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of accessory genes in Salmonella requires the presence of the Gre factors</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>116</volume><issue>1</issue><spage>110777</spage><pages>110777-</pages><artnum>110777</artnum><issn>0888-7543</issn><issn>1089-8646</issn><eissn>1089-8646</eissn><abstract>Genomic studies with Salmonella enterica serovar Typhimurium reveal a crucial role of horizontal gene transfer (HGT) in the acquisition of accessory cellular functions involved in host-interaction. Many virulence genes are located in genomic islands, plasmids and prophages. GreA and GreB proteins, Gre factors, interact transiently with the RNA polymerase alleviating backtracked complexes during transcription elongation. The overall effect of Gre factors depletion in Salmonella expression profile was studied. Both proteins are functionally redundant since only when both Gre factors were depleted a major effect in gene expression was detected. Remarkably, the accessory gene pool is particularly sensitive to the lack of Gre factors, with 18.6% of accessory genes stimulated by the Gre factors versus 4.4% of core genome genes. Gre factors involvement is particularly relevant for the expression of genes located in genomic islands. Our data reveal that Gre factors are required for the expression of accessory genes.
•Gre factors are required for transcriptional expression of accessory genes in Salmonella.•Expression of SPI-2 and SPI-2 coregulated genes, involved in intracellular survival, are highly dependent on the Gre factors.•GreA and GreB are functionally redundant in Salmonella.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38163572</pmid><doi>10.1016/j.ygeno.2023.110777</doi><oa>free_for_read</oa></addata></record> |
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subjects | Accessory genes Gene expression Gre factors Salmonella Transcription elongation Virulence |
title | Expression of accessory genes in Salmonella requires the presence of the Gre factors |
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