Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins

Disease models based on induced pluripotent stem cells (iPSCs) are in high demand because of their physiological adequacy and well-reproducibility of the pathological phenotype. Nowadays, the most common approach to generate iPSCs is the reprogramming of somatic cells using vectors based on lentivir...

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Veröffentlicht in:Biochimie 2024-07, Vol.222, p.63-71
Hauptverfasser: Oshkolova, Arina A., Grekhnev, Dmitriy A., Kruchinina, Anna A., Belikova, Lilia D., Volovikov, Egor A., Lebedeva, Olga S., Bogomazova, Alexandra N., Vigont, Vladimir A., Lagarkova, Maria A., Kaznacheyeva, Elena V.
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container_title Biochimie
container_volume 222
creator Oshkolova, Arina A.
Grekhnev, Dmitriy A.
Kruchinina, Anna A.
Belikova, Lilia D.
Volovikov, Egor A.
Lebedeva, Olga S.
Bogomazova, Alexandra N.
Vigont, Vladimir A.
Lagarkova, Maria A.
Kaznacheyeva, Elena V.
description Disease models based on induced pluripotent stem cells (iPSCs) are in high demand because of their physiological adequacy and well-reproducibility of the pathological phenotype. Nowadays, the most common approach to generate iPSCs is the reprogramming of somatic cells using vectors based on lentivirus or Sendai virus. We have previously shown impairments of calcium signaling including store-operated calcium entry in Huntington's disease-specific iPSCs-based GABA-ergic medium spiny neurons. However, different approaches for iPSCs generation make it difficult to compare the models since the mechanism of reprogramming may influence the electrophysiological properties of the terminally differentiated neurons. Here, we have studied the features of calcium homeostasis in GABA-ergic medium spiny neurons differentiated from iPSCs obtained from fibroblasts of the same donor using different methods. Our data demonstrated that there were no significant differences neither in calcium influx through the store-operated channels, nor in the levels of proteins activating this type of calcium entry in neurons differentiated from iPSCs generated with lenti- and Sendai viruses-based approaches. We also found no differences in voltage-gated calcium entry for these neurons. Thus, we clearly showed that various methods of cell reprogramming result in similar deregulations in neuronal calcium signaling which substantiates the ability to combine the experimental data on functional studies of ion channels in models based on iPSCs obtained by different methods and expands the prospects for the use of biobanking. •Different methods of iPSCs generation result in similar neuronal calcium signaling.•Data obtained with different iPSCs-based models can be combined.•Calcium alterations in HD-specific neurons do not depend on iPSCs generation method.
doi_str_mv 10.1016/j.biochi.2023.12.011
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source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Calcium - metabolism
Calcium Signaling
Cell Differentiation
Cells, Cultured
Fibroblasts - cytology
Fibroblasts - metabolism
Fluorescent calcium imaging
GABAergic Neurons - cytology
GABAergic Neurons - metabolism
Humans
Huntington's disease
Induced pluripotent stem cells
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Integrative reprogramming methods
Lentivirus - genetics
Medium Spiny Neurons
Neurons - cytology
Neurons - metabolism
Non-integrative reprogramming methods
Sendai virus
Store-operated calcium entry
Voltage-gated calcium entry
title Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins
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