Mechanistic insights into the survival curve of HeLa cells with a short shoulder and their S phase-specific sensitivity
Abstract HeLa cells are a cell line with two unique cellular features: a short-shouldered survival curve and two peaks of radioresistance during the cell cycle phase, while their underlying mechanisms remain unclear. We herein proposed that these radiobiological features are due to a common mechanis...
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| Veröffentlicht in: | Journal of radiation research 2024-03, Vol.65 (2), p.256-258 |
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| container_title | Journal of radiation research |
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| creator | Komatsu, Kenshi Tauchi, Hiroshi |
| description | Abstract
HeLa cells are a cell line with two unique cellular features: a short-shouldered survival curve and two peaks of radioresistance during the cell cycle phase, while their underlying mechanisms remain unclear. We herein proposed that these radiobiological features are due to a common mechanism by which radiation suppresses homologous recombination repair (HRR) in a dose-dependent manner. This radio-suppression of HRR is mediated by an intra-S checkpoint and reduces survivals of cells in S phase, especially early S phase, resulting in both short shoulder and radioresistance with two peaks in the cell cycle. This new explanation may not be limited to HeLa cells since a similar close association of these features is also observed in other type of cells. |
| doi_str_mv | 10.1093/jrr/rrad097 |
| format | Article |
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HeLa cells are a cell line with two unique cellular features: a short-shouldered survival curve and two peaks of radioresistance during the cell cycle phase, while their underlying mechanisms remain unclear. We herein proposed that these radiobiological features are due to a common mechanism by which radiation suppresses homologous recombination repair (HRR) in a dose-dependent manner. This radio-suppression of HRR is mediated by an intra-S checkpoint and reduces survivals of cells in S phase, especially early S phase, resulting in both short shoulder and radioresistance with two peaks in the cell cycle. This new explanation may not be limited to HeLa cells since a similar close association of these features is also observed in other type of cells.</description><identifier>ISSN: 0449-3060</identifier><identifier>EISSN: 1349-9157</identifier><identifier>DOI: 10.1093/jrr/rrad097</identifier><identifier>PMID: 38151954</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Cell Cycle ; Cell Survival ; DNA Repair ; HeLa Cells ; Humans ; Radiation ; Radiation Tolerance ; S Phase ; Shoulder</subject><ispartof>Journal of radiation research, 2024-03, Vol.65 (2), p.256-258</ispartof><rights>The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. 2023</rights><rights>The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology.</rights><rights>COPYRIGHT 2024 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c382t-6b20baa1d8185f891ea0704e1b73c18cfcd32944f2efb043707474997813146d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,1598,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38151954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Komatsu, Kenshi</creatorcontrib><creatorcontrib>Tauchi, Hiroshi</creatorcontrib><title>Mechanistic insights into the survival curve of HeLa cells with a short shoulder and their S phase-specific sensitivity</title><title>Journal of radiation research</title><addtitle>J Radiat Res</addtitle><description>Abstract
HeLa cells are a cell line with two unique cellular features: a short-shouldered survival curve and two peaks of radioresistance during the cell cycle phase, while their underlying mechanisms remain unclear. We herein proposed that these radiobiological features are due to a common mechanism by which radiation suppresses homologous recombination repair (HRR) in a dose-dependent manner. This radio-suppression of HRR is mediated by an intra-S checkpoint and reduces survivals of cells in S phase, especially early S phase, resulting in both short shoulder and radioresistance with two peaks in the cell cycle. This new explanation may not be limited to HeLa cells since a similar close association of these features is also observed in other type of cells.</description><subject>Cell Cycle</subject><subject>Cell Survival</subject><subject>DNA Repair</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Radiation</subject><subject>Radiation Tolerance</subject><subject>S Phase</subject><subject>Shoulder</subject><issn>0449-3060</issn><issn>1349-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><sourceid>EIF</sourceid><recordid>eNp9kdFr1TAUxoMo7jp98l0CwhCk20mTNs3jGOoGd-xBfQ5perJm9LY1Se_Yf7-UexUEkUDyEX7fdw58hLxncM5A8YuHEC5CMB0o-YJsGBeqUKySL8kGRNYcajghb2J8ACglVPCanPCGVUxVYkMeb9H2ZvQxeUv9GP19n2IWaaKpRxqXsPd7M1CbBdLJ0WvcGmpxGCJ99KmnhsZ-Cmm9l6HDQM3YrVYf6Hc69yZiEWe03uX8iHlA8nufnt6SV84MEd8d31Py8-uXH1fXxfbu283V5bawvClTUbcltMawrmFN5RrF0IAEgayV3LLGOtvxUgnhSnQtCC5BCimUkg3jTNQdPyWfDrlzmH4tGJPe-biub0aclqhLBZKpGgRk9OMBvTcDaj-6KQVjV1xfykbVQnDGMnX-DyqfDnfeTiM6n___Mnw-GGyYYgzo9Bz8zoQnzUCvBepcoD4WmOkPx32XdofdH_Z3Yxk4OwDTMv836Rln3aQ7</recordid><startdate>20240322</startdate><enddate>20240322</enddate><creator>Komatsu, Kenshi</creator><creator>Tauchi, Hiroshi</creator><general>Oxford University Press</general><scope>TOX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240322</creationdate><title>Mechanistic insights into the survival curve of HeLa cells with a short shoulder and their S phase-specific sensitivity</title><author>Komatsu, Kenshi ; Tauchi, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-6b20baa1d8185f891ea0704e1b73c18cfcd32944f2efb043707474997813146d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Cell Cycle</topic><topic>Cell Survival</topic><topic>DNA Repair</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Radiation</topic><topic>Radiation Tolerance</topic><topic>S Phase</topic><topic>Shoulder</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Komatsu, Kenshi</creatorcontrib><creatorcontrib>Tauchi, Hiroshi</creatorcontrib><collection>Oxford Journals Open Access Collection</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Komatsu, Kenshi</au><au>Tauchi, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanistic insights into the survival curve of HeLa cells with a short shoulder and their S phase-specific sensitivity</atitle><jtitle>Journal of radiation research</jtitle><addtitle>J Radiat Res</addtitle><date>2024-03-22</date><risdate>2024</risdate><volume>65</volume><issue>2</issue><spage>256</spage><epage>258</epage><pages>256-258</pages><issn>0449-3060</issn><eissn>1349-9157</eissn><abstract>Abstract
HeLa cells are a cell line with two unique cellular features: a short-shouldered survival curve and two peaks of radioresistance during the cell cycle phase, while their underlying mechanisms remain unclear. We herein proposed that these radiobiological features are due to a common mechanism by which radiation suppresses homologous recombination repair (HRR) in a dose-dependent manner. This radio-suppression of HRR is mediated by an intra-S checkpoint and reduces survivals of cells in S phase, especially early S phase, resulting in both short shoulder and radioresistance with two peaks in the cell cycle. This new explanation may not be limited to HeLa cells since a similar close association of these features is also observed in other type of cells.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>38151954</pmid><doi>10.1093/jrr/rrad097</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of radiation research, 2024-03, Vol.65 (2), p.256-258 |
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| language | eng |
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| source | Oxford Journals Open Access Collection; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Cell Cycle Cell Survival DNA Repair HeLa Cells Humans Radiation Radiation Tolerance S Phase Shoulder |
| title | Mechanistic insights into the survival curve of HeLa cells with a short shoulder and their S phase-specific sensitivity |
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