Genomic insight into the insecticidal potential of a new Pseudomonas chlororaphis isolate
Abstract Pseudomonas fluorescens group, such as Pseudomonas protegens and Pseudomonas chlororaphis, can be utilized as insect-killing agents. Most insecticidal Pseudomonas described so far have high toxicity for insects of the order Lepidoptera. In this study, Pseudomonas strain PcR3-3 was isolated...
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| Veröffentlicht in: | Journal of economic entomology 2024-02, Vol.117 (1), p.82-92 |
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| creator | Wang, Haitao Zhang, Yali Dai, Dadong Fu, Jinqiu Sung Kim, Dae Li, Shengchun Zhang, Jiang Wang, Yong Zhang, Fengjuan |
| description | Abstract
Pseudomonas fluorescens group, such as Pseudomonas protegens and Pseudomonas chlororaphis, can be utilized as insect-killing agents. Most insecticidal Pseudomonas described so far have high toxicity for insects of the order Lepidoptera. In this study, Pseudomonas strain PcR3-3 was isolated from the willow root. It showed a high mortality for the coleopteran species Plagiodera versicolora (Coleoptera: Chrysomelidae), but not for the lepidopteran Helicoverpa armigera. Strain PcR3-3 displayed high colonization ability in the P. versicolora compared with P. chlororaphis PCL1391, indicating that the insecticidal activities correlated with the colonization ability of Pseudomonas strain in the host. Phylogenetic analysis of the genome revealed that PcR3-3 belonged to P. chlororaphis subsp. aureofaciens. Numerous insecticidal protein-encoding genes, typical biosynthetic gene clusters for some insecticidal metabolite and type VI secretion system, known to be involved in insect pathogenicity, were present in the P. chlororaphis PcR3-3 genome. However, the insecticidal toxin Fit-encoding gene which commonly presents in P. chlororaphis, was not found in the P. chlororaphis PcR3-3 genome. Furthermore, there are some divergent insecticidal genes between P. chlororaphis PcR3-3 and P. chlororaphis PCL1391. This finding implies that P. chlororaphis PcR3-3 is a promising biocontrol agent for pest management applications. The P. chlororaphis–P. versicolora association can be used as a model system to study the interaction between Pseudomonas and coleopteran insects. |
| doi_str_mv | 10.1093/jee/toad232 |
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Pseudomonas fluorescens group, such as Pseudomonas protegens and Pseudomonas chlororaphis, can be utilized as insect-killing agents. Most insecticidal Pseudomonas described so far have high toxicity for insects of the order Lepidoptera. In this study, Pseudomonas strain PcR3-3 was isolated from the willow root. It showed a high mortality for the coleopteran species Plagiodera versicolora (Coleoptera: Chrysomelidae), but not for the lepidopteran Helicoverpa armigera. Strain PcR3-3 displayed high colonization ability in the P. versicolora compared with P. chlororaphis PCL1391, indicating that the insecticidal activities correlated with the colonization ability of Pseudomonas strain in the host. Phylogenetic analysis of the genome revealed that PcR3-3 belonged to P. chlororaphis subsp. aureofaciens. Numerous insecticidal protein-encoding genes, typical biosynthetic gene clusters for some insecticidal metabolite and type VI secretion system, known to be involved in insect pathogenicity, were present in the P. chlororaphis PcR3-3 genome. However, the insecticidal toxin Fit-encoding gene which commonly presents in P. chlororaphis, was not found in the P. chlororaphis PcR3-3 genome. Furthermore, there are some divergent insecticidal genes between P. chlororaphis PcR3-3 and P. chlororaphis PCL1391. This finding implies that P. chlororaphis PcR3-3 is a promising biocontrol agent for pest management applications. The P. chlororaphis–P. versicolora association can be used as a model system to study the interaction between Pseudomonas and coleopteran insects.</description><identifier>ISSN: 0022-0493</identifier><identifier>ISSN: 1938-291X</identifier><identifier>EISSN: 1938-291X</identifier><identifier>DOI: 10.1093/jee/toad232</identifier><identifier>PMID: 38146627</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Animals ; Biological control ; Coleoptera ; Colonization ; Gene clusters ; Genes ; Genomes ; Genomics ; Insecta ; Insecticides - pharmacology ; Insects ; Metabolites ; Pathogenicity ; Pathogens ; Pest control ; Phylogeny ; Pseudomonas ; Pseudomonas chlororaphis ; Pseudomonas chlororaphis - genetics ; Toxicity ; Toxins</subject><ispartof>Journal of economic entomology, 2024-02, Vol.117 (1), p.82-92</ispartof><rights>The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. 2023</rights><rights>The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c306t-7507220a2547f8736fa321c2d291c428ffdea79127cceb880be6a725279fd7f63</cites><orcidid>0000-0002-6727-7974 ; 0000-0002-4579-2094 ; 0000-0003-3949-3765</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38146627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Haitao</creatorcontrib><creatorcontrib>Zhang, Yali</creatorcontrib><creatorcontrib>Dai, Dadong</creatorcontrib><creatorcontrib>Fu, Jinqiu</creatorcontrib><creatorcontrib>Sung Kim, Dae</creatorcontrib><creatorcontrib>Li, Shengchun</creatorcontrib><creatorcontrib>Zhang, Jiang</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><creatorcontrib>Zhang, Fengjuan</creatorcontrib><title>Genomic insight into the insecticidal potential of a new Pseudomonas chlororaphis isolate</title><title>Journal of economic entomology</title><addtitle>J Econ Entomol</addtitle><description>Abstract
Pseudomonas fluorescens group, such as Pseudomonas protegens and Pseudomonas chlororaphis, can be utilized as insect-killing agents. Most insecticidal Pseudomonas described so far have high toxicity for insects of the order Lepidoptera. In this study, Pseudomonas strain PcR3-3 was isolated from the willow root. It showed a high mortality for the coleopteran species Plagiodera versicolora (Coleoptera: Chrysomelidae), but not for the lepidopteran Helicoverpa armigera. Strain PcR3-3 displayed high colonization ability in the P. versicolora compared with P. chlororaphis PCL1391, indicating that the insecticidal activities correlated with the colonization ability of Pseudomonas strain in the host. Phylogenetic analysis of the genome revealed that PcR3-3 belonged to P. chlororaphis subsp. aureofaciens. Numerous insecticidal protein-encoding genes, typical biosynthetic gene clusters for some insecticidal metabolite and type VI secretion system, known to be involved in insect pathogenicity, were present in the P. chlororaphis PcR3-3 genome. However, the insecticidal toxin Fit-encoding gene which commonly presents in P. chlororaphis, was not found in the P. chlororaphis PcR3-3 genome. Furthermore, there are some divergent insecticidal genes between P. chlororaphis PcR3-3 and P. chlororaphis PCL1391. This finding implies that P. chlororaphis PcR3-3 is a promising biocontrol agent for pest management applications. The P. chlororaphis–P. versicolora association can be used as a model system to study the interaction between Pseudomonas and coleopteran insects.</description><subject>Animals</subject><subject>Biological control</subject><subject>Coleoptera</subject><subject>Colonization</subject><subject>Gene clusters</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Insecta</subject><subject>Insecticides - pharmacology</subject><subject>Insects</subject><subject>Metabolites</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Pest control</subject><subject>Phylogeny</subject><subject>Pseudomonas</subject><subject>Pseudomonas chlororaphis</subject><subject>Pseudomonas chlororaphis - genetics</subject><subject>Toxicity</subject><subject>Toxins</subject><issn>0022-0493</issn><issn>1938-291X</issn><issn>1938-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp90MFLwzAUBvAgipvTk3cpCCJIXfLSNu1Rhk5hoAcFPZUsfXEZbVObFPG_N2PTgwdPLzx-fOR9hJwyes1owadrxKm3sgIOe2TMCp7HULDXfTKmFCCmScFH5Mi5NaUsA0YPyYjnLMkyEGPyNsfWNkZFpnXmfeXD9DbyK9wsUHmjTCXrqLMeW2_Cy-pIRi1-Rk8Oh8o2tpUuUqva9raX3cq4yDhbS4_H5EDL2uHJbk7Iy93t8-w-XjzOH2Y3i1hxmvlYpFQAUAlpInQueKYlB6agCjeoBHKtK5SiYCCUwmWe0yVmUkAKotCV0BmfkMttbtfbjwGdLxvjFNa1bNEOroSCZkzkiRCBnv-hazv0bfhdUGkKnKY8D-pqq1RvnetRl11vGtl_lYyWm8bL0Hi5azzos13msGyw-rU_FQdwsQV26P5N-gaVPIoc</recordid><startdate>20240212</startdate><enddate>20240212</enddate><creator>Wang, Haitao</creator><creator>Zhang, Yali</creator><creator>Dai, Dadong</creator><creator>Fu, Jinqiu</creator><creator>Sung Kim, Dae</creator><creator>Li, Shengchun</creator><creator>Zhang, Jiang</creator><creator>Wang, Yong</creator><creator>Zhang, Fengjuan</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6727-7974</orcidid><orcidid>https://orcid.org/0000-0002-4579-2094</orcidid><orcidid>https://orcid.org/0000-0003-3949-3765</orcidid></search><sort><creationdate>20240212</creationdate><title>Genomic insight into the insecticidal potential of a new Pseudomonas chlororaphis isolate</title><author>Wang, Haitao ; Zhang, Yali ; Dai, Dadong ; Fu, Jinqiu ; Sung Kim, Dae ; Li, Shengchun ; Zhang, Jiang ; Wang, Yong ; Zhang, Fengjuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-7507220a2547f8736fa321c2d291c428ffdea79127cceb880be6a725279fd7f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Biological control</topic><topic>Coleoptera</topic><topic>Colonization</topic><topic>Gene clusters</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Insecta</topic><topic>Insecticides - pharmacology</topic><topic>Insects</topic><topic>Metabolites</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Pest control</topic><topic>Phylogeny</topic><topic>Pseudomonas</topic><topic>Pseudomonas chlororaphis</topic><topic>Pseudomonas chlororaphis - genetics</topic><topic>Toxicity</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Haitao</creatorcontrib><creatorcontrib>Zhang, Yali</creatorcontrib><creatorcontrib>Dai, Dadong</creatorcontrib><creatorcontrib>Fu, Jinqiu</creatorcontrib><creatorcontrib>Sung Kim, Dae</creatorcontrib><creatorcontrib>Li, Shengchun</creatorcontrib><creatorcontrib>Zhang, Jiang</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><creatorcontrib>Zhang, Fengjuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of economic entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Haitao</au><au>Zhang, Yali</au><au>Dai, Dadong</au><au>Fu, Jinqiu</au><au>Sung Kim, Dae</au><au>Li, Shengchun</au><au>Zhang, Jiang</au><au>Wang, Yong</au><au>Zhang, Fengjuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic insight into the insecticidal potential of a new Pseudomonas chlororaphis isolate</atitle><jtitle>Journal of economic entomology</jtitle><addtitle>J Econ Entomol</addtitle><date>2024-02-12</date><risdate>2024</risdate><volume>117</volume><issue>1</issue><spage>82</spage><epage>92</epage><pages>82-92</pages><issn>0022-0493</issn><issn>1938-291X</issn><eissn>1938-291X</eissn><abstract>Abstract
Pseudomonas fluorescens group, such as Pseudomonas protegens and Pseudomonas chlororaphis, can be utilized as insect-killing agents. Most insecticidal Pseudomonas described so far have high toxicity for insects of the order Lepidoptera. In this study, Pseudomonas strain PcR3-3 was isolated from the willow root. It showed a high mortality for the coleopteran species Plagiodera versicolora (Coleoptera: Chrysomelidae), but not for the lepidopteran Helicoverpa armigera. Strain PcR3-3 displayed high colonization ability in the P. versicolora compared with P. chlororaphis PCL1391, indicating that the insecticidal activities correlated with the colonization ability of Pseudomonas strain in the host. Phylogenetic analysis of the genome revealed that PcR3-3 belonged to P. chlororaphis subsp. aureofaciens. Numerous insecticidal protein-encoding genes, typical biosynthetic gene clusters for some insecticidal metabolite and type VI secretion system, known to be involved in insect pathogenicity, were present in the P. chlororaphis PcR3-3 genome. However, the insecticidal toxin Fit-encoding gene which commonly presents in P. chlororaphis, was not found in the P. chlororaphis PcR3-3 genome. Furthermore, there are some divergent insecticidal genes between P. chlororaphis PcR3-3 and P. chlororaphis PCL1391. This finding implies that P. chlororaphis PcR3-3 is a promising biocontrol agent for pest management applications. The P. chlororaphis–P. versicolora association can be used as a model system to study the interaction between Pseudomonas and coleopteran insects.</abstract><cop>US</cop><pub>Oxford University Press</pub><pmid>38146627</pmid><doi>10.1093/jee/toad232</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6727-7974</orcidid><orcidid>https://orcid.org/0000-0002-4579-2094</orcidid><orcidid>https://orcid.org/0000-0003-3949-3765</orcidid></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Animals Biological control Coleoptera Colonization Gene clusters Genes Genomes Genomics Insecta Insecticides - pharmacology Insects Metabolites Pathogenicity Pathogens Pest control Phylogeny Pseudomonas Pseudomonas chlororaphis Pseudomonas chlororaphis - genetics Toxicity Toxins |
| title | Genomic insight into the insecticidal potential of a new Pseudomonas chlororaphis isolate |
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