Alternative amplicon-PCR protocol for maximizing bacterial and fungal sequencing in low-biomass samples

Determining bacterial and fungal communities from low-biomass samples remains a challenge for high-throughput sequencing. Due to the low microbial load and host contamination, some sites, including the female upper reproductive tract and the lower respiratory tract, were even considered sterile unti...

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Veröffentlicht in:	Analytical biochemistry 2024-04, Vol.687, p.115449-115449, Article 115449
Hauptverfasser:	Merker Breyer, Gabriela, De Carli, Silvia, Rocha Jacques Da Silva, Maria Eduarda, Dias, Maria Eduarda, Muterle Varela, Ana Paula, Bertoni Mann, Michele, Frazzon, Jeverson, Quoos Mayer, Fabiana, Góes Neto, Aristóteles, Maboni Siqueira, Franciele
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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