Transfection of Unmodified MicroRNA Using Monolayer-Coated Au Nanoparticles as Gene-Delivery Vehicles

Transfection of Unmodified MicroRNA Using Monolayer-Coated Au Nanoparticles as Gene-Delivery Vehicles

This article describes a monolayer-coated gold nanoparticle-based transfection system for the delivery of microRNA (miRNA) into human osteosarcoma (HOS) cells. Two distinct ammonium-terminated adsorbates were used in this study, which provided a platform for ionic bonding of the miRNA onto gold nano...

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Veröffentlicht in:ACS applied bio materials 2024-01, Vol.7 (1), p.230-237
Hauptverfasser: Hoang, Johnson, Patil, Sagar L., Srinoi, Pannaree, Liu, Tingting, Marquez, Maria D., Khantamat, Orawan, Tuntiwechapikul, Wirote, Gunaratne, Preethi H., Lee, T. Randall
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container_issue 1
container_start_page 230
container_title ACS applied bio materials
container_volume 7
creator Hoang, Johnson
Patil, Sagar L.
Srinoi, Pannaree
Liu, Tingting
Marquez, Maria D.
Khantamat, Orawan
Tuntiwechapikul, Wirote
Gunaratne, Preethi H.
Lee, T. Randall
description This article describes a monolayer-coated gold nanoparticle-based transfection system for the delivery of microRNA (miRNA) into human osteosarcoma (HOS) cells. Two distinct ammonium-terminated adsorbates were used in this study, which provided a platform for ionic bonding of the miRNA onto gold nanoparticles (AuNPs). The custom-designed monolayer-coated gold nanoparticles were characterized by dynamic light scattering, gel mobility shift assay, transmission electron microscopy, ultraviolet–visible spectrometry, zeta potential, and X-ray photoelectron spectroscopy. The miRNA-loaded gold nanoparticles were transfected, and the level of intracellular miRNA delivered and taken up by cells was measured by Taqman qPCR. The overall analysis indicated a successful delivery of miRNA into the HOS cells at an ∼11,000-fold increase compared to nontreated cells.
doi_str_mv 10.1021/acsabm.3c00837
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title Transfection of Unmodified MicroRNA Using Monolayer-Coated Au Nanoparticles as Gene-Delivery Vehicles
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