Retrospective assessment of immunologic and histologic heterogeneity in granuloma annulare by cytokine staining
Background Type 1 (Th1) and Type 2 (Th2) immunity have both been implicated in granuloma annulare (GA). To what extent these pathways contribute to clinical/histologic heterogeneity and/or distinct disease endotypes remains unexplored. Methods We retrospectively analyzed 30 GA biopsies with either p...
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| Veröffentlicht in: | International journal of dermatology 2024-05, Vol.63 (5), p.655-659 |
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| creator | Hwang, Erica Lee, Trinity Okifo, Kevin Murphy, Michael Damsky, William |
| description | Background
Type 1 (Th1) and Type 2 (Th2) immunity have both been implicated in granuloma annulare (GA). To what extent these pathways contribute to clinical/histologic heterogeneity and/or distinct disease endotypes remains unexplored.
Methods
We retrospectively analyzed 30 GA biopsies with either palisaded or interstitial histology with and without eosinophils. We performed RNA in situ hybridization to assess how markers of Type 1 (interferon gamma), Type 2 (interleukin [IL]4, IL13, IL5), and Type 3 (IL17A) immunity in GA compared with canonical inflammatory disorders and whether markers correlated with histology. We analyzed another cohort of 14 patients who had multiple biopsies across anatomic space and time for individual conservation of histologic features.
Results
Interferon (IFN)G staining is highest in GA relative to other cytokines. Type 2 cytokine staining is less prominent, with IL4 increased in interstitial pattern cases. Eosinophils did not correlate with Type 2 markers. Patients with multiple biopsies display intrapatient variability in histology.
Conclusion
Type 1 inflammation predominates over Type 2 inflammation in GA irrespective of histologic pattern. Distinct disease endotypes were not detected. |
| doi_str_mv | 10.1111/ijd.16998 |
| format | Article |
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Type 1 (Th1) and Type 2 (Th2) immunity have both been implicated in granuloma annulare (GA). To what extent these pathways contribute to clinical/histologic heterogeneity and/or distinct disease endotypes remains unexplored.
Methods
We retrospectively analyzed 30 GA biopsies with either palisaded or interstitial histology with and without eosinophils. We performed RNA in situ hybridization to assess how markers of Type 1 (interferon gamma), Type 2 (interleukin [IL]4, IL13, IL5), and Type 3 (IL17A) immunity in GA compared with canonical inflammatory disorders and whether markers correlated with histology. We analyzed another cohort of 14 patients who had multiple biopsies across anatomic space and time for individual conservation of histologic features.
Results
Interferon (IFN)G staining is highest in GA relative to other cytokines. Type 2 cytokine staining is less prominent, with IL4 increased in interstitial pattern cases. Eosinophils did not correlate with Type 2 markers. Patients with multiple biopsies display intrapatient variability in histology.
Conclusion
Type 1 inflammation predominates over Type 2 inflammation in GA irrespective of histologic pattern. Distinct disease endotypes were not detected.</description><identifier>ISSN: 0011-9059</identifier><identifier>EISSN: 1365-4632</identifier><identifier>DOI: 10.1111/ijd.16998</identifier><identifier>PMID: 38135676</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; Biopsy ; Cytokines ; Cytokines - metabolism ; Eosinophils ; Eosinophils - immunology ; Eosinophils - pathology ; Female ; Granuloma ; Granuloma Annulare - diagnosis ; Granuloma Annulare - immunology ; Granuloma Annulare - pathology ; Granulomas ; Heterogeneity ; Histology ; Humans ; Hybridization ; Immunity ; In Situ Hybridization ; Inflammatory diseases ; Interferon ; Interferon-gamma ; Interleukin-17 - metabolism ; Interleukin-4 ; Interleukin-5 ; Leukocytes (eosinophilic) ; Lymphocytes T ; Male ; Middle Aged ; Retrospective Studies ; Skin - immunology ; Skin - pathology ; Staining ; Staining and Labeling ; Th1 Cells - immunology ; Th2 Cells - immunology ; Young Adult</subject><ispartof>International journal of dermatology, 2024-05, Vol.63 (5), p.655-659</ispartof><rights>2023 the International Society of Dermatology.</rights><rights>International Journal of Dermatology © 2024 International Society of Dermatology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3138-1de3d89a22fc1125d4c84c86b32cc508d628bb789f1ca02997570ca7556f9ec73</cites><orcidid>0000-0002-5728-9875 ; 0000-0001-6494-7250</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fijd.16998$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fijd.16998$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38135676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, Erica</creatorcontrib><creatorcontrib>Lee, Trinity</creatorcontrib><creatorcontrib>Okifo, Kevin</creatorcontrib><creatorcontrib>Murphy, Michael</creatorcontrib><creatorcontrib>Damsky, William</creatorcontrib><title>Retrospective assessment of immunologic and histologic heterogeneity in granuloma annulare by cytokine staining</title><title>International journal of dermatology</title><addtitle>Int J Dermatol</addtitle><description>Background
Type 1 (Th1) and Type 2 (Th2) immunity have both been implicated in granuloma annulare (GA). To what extent these pathways contribute to clinical/histologic heterogeneity and/or distinct disease endotypes remains unexplored.
Methods
We retrospectively analyzed 30 GA biopsies with either palisaded or interstitial histology with and without eosinophils. We performed RNA in situ hybridization to assess how markers of Type 1 (interferon gamma), Type 2 (interleukin [IL]4, IL13, IL5), and Type 3 (IL17A) immunity in GA compared with canonical inflammatory disorders and whether markers correlated with histology. We analyzed another cohort of 14 patients who had multiple biopsies across anatomic space and time for individual conservation of histologic features.
Results
Interferon (IFN)G staining is highest in GA relative to other cytokines. Type 2 cytokine staining is less prominent, with IL4 increased in interstitial pattern cases. Eosinophils did not correlate with Type 2 markers. Patients with multiple biopsies display intrapatient variability in histology.
Conclusion
Type 1 inflammation predominates over Type 2 inflammation in GA irrespective of histologic pattern. Distinct disease endotypes were not detected.</description><subject>Adult</subject><subject>Aged</subject><subject>Biopsy</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Eosinophils</subject><subject>Eosinophils - immunology</subject><subject>Eosinophils - pathology</subject><subject>Female</subject><subject>Granuloma</subject><subject>Granuloma Annulare - diagnosis</subject><subject>Granuloma Annulare - immunology</subject><subject>Granuloma Annulare - pathology</subject><subject>Granulomas</subject><subject>Heterogeneity</subject><subject>Histology</subject><subject>Humans</subject><subject>Hybridization</subject><subject>Immunity</subject><subject>In Situ Hybridization</subject><subject>Inflammatory diseases</subject><subject>Interferon</subject><subject>Interferon-gamma</subject><subject>Interleukin-17 - metabolism</subject><subject>Interleukin-4</subject><subject>Interleukin-5</subject><subject>Leukocytes (eosinophilic)</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Retrospective Studies</subject><subject>Skin - immunology</subject><subject>Skin - pathology</subject><subject>Staining</subject><subject>Staining and Labeling</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - immunology</subject><subject>Young Adult</subject><issn>0011-9059</issn><issn>1365-4632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV9rFTEQxYNU7G31wS8ggb7Yh23z5yabPJaqbaUgiD6HbHb2Ntfd5DbJVvbbG73XPggOAzMDPw7DOQi9peSC1rr02_6CSq3VC7SiXIpmLTk7QitCKG00EfoYneS8rSdndP0KHXNFuZCtXKH4FUqKeQeu-CfANmfIeYJQcBywn6Y5xDFuvMM29PjB53I4H6BAihsI4MuCfcCbZMM8xslWsi42Ae4W7JYSf_gAOBfrgw-b1-jlYMcMbw7zFH3_9PHb9W1z_-Xm7vrqvnGcctXQHnivtGVscJQy0a-dqi07zpwTRPWSqa5rlR6os4Rp3YqWONsKIQcNruWn6P1ed5fi4wy5mMlnB-NoA8Q5G1ZtEYwSoip69g-6jXMK9TvDyVpqqUjLK3W-p1y1KycYzC75yabFUGJ-p2BqCuZPCpV9d1Ccuwn6Z_Kv7RW43AM__QjL_5XM3ecPe8lf17iSwg</recordid><startdate>202405</startdate><enddate>202405</enddate><creator>Hwang, Erica</creator><creator>Lee, Trinity</creator><creator>Okifo, Kevin</creator><creator>Murphy, Michael</creator><creator>Damsky, William</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5728-9875</orcidid><orcidid>https://orcid.org/0000-0001-6494-7250</orcidid></search><sort><creationdate>202405</creationdate><title>Retrospective assessment of immunologic and histologic heterogeneity in granuloma annulare by cytokine staining</title><author>Hwang, Erica ; Lee, Trinity ; Okifo, Kevin ; Murphy, Michael ; Damsky, William</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3138-1de3d89a22fc1125d4c84c86b32cc508d628bb789f1ca02997570ca7556f9ec73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biopsy</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Eosinophils</topic><topic>Eosinophils - immunology</topic><topic>Eosinophils - pathology</topic><topic>Female</topic><topic>Granuloma</topic><topic>Granuloma Annulare - diagnosis</topic><topic>Granuloma Annulare - immunology</topic><topic>Granuloma Annulare - pathology</topic><topic>Granulomas</topic><topic>Heterogeneity</topic><topic>Histology</topic><topic>Humans</topic><topic>Hybridization</topic><topic>Immunity</topic><topic>In Situ Hybridization</topic><topic>Inflammatory diseases</topic><topic>Interferon</topic><topic>Interferon-gamma</topic><topic>Interleukin-17 - metabolism</topic><topic>Interleukin-4</topic><topic>Interleukin-5</topic><topic>Leukocytes (eosinophilic)</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Retrospective Studies</topic><topic>Skin - immunology</topic><topic>Skin - pathology</topic><topic>Staining</topic><topic>Staining and Labeling</topic><topic>Th1 Cells - immunology</topic><topic>Th2 Cells - immunology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, Erica</creatorcontrib><creatorcontrib>Lee, Trinity</creatorcontrib><creatorcontrib>Okifo, Kevin</creatorcontrib><creatorcontrib>Murphy, Michael</creatorcontrib><creatorcontrib>Damsky, William</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, Erica</au><au>Lee, Trinity</au><au>Okifo, Kevin</au><au>Murphy, Michael</au><au>Damsky, William</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retrospective assessment of immunologic and histologic heterogeneity in granuloma annulare by cytokine staining</atitle><jtitle>International journal of dermatology</jtitle><addtitle>Int J Dermatol</addtitle><date>2024-05</date><risdate>2024</risdate><volume>63</volume><issue>5</issue><spage>655</spage><epage>659</epage><pages>655-659</pages><issn>0011-9059</issn><eissn>1365-4632</eissn><abstract>Background
Type 1 (Th1) and Type 2 (Th2) immunity have both been implicated in granuloma annulare (GA). To what extent these pathways contribute to clinical/histologic heterogeneity and/or distinct disease endotypes remains unexplored.
Methods
We retrospectively analyzed 30 GA biopsies with either palisaded or interstitial histology with and without eosinophils. We performed RNA in situ hybridization to assess how markers of Type 1 (interferon gamma), Type 2 (interleukin [IL]4, IL13, IL5), and Type 3 (IL17A) immunity in GA compared with canonical inflammatory disorders and whether markers correlated with histology. We analyzed another cohort of 14 patients who had multiple biopsies across anatomic space and time for individual conservation of histologic features.
Results
Interferon (IFN)G staining is highest in GA relative to other cytokines. Type 2 cytokine staining is less prominent, with IL4 increased in interstitial pattern cases. Eosinophils did not correlate with Type 2 markers. Patients with multiple biopsies display intrapatient variability in histology.
Conclusion
Type 1 inflammation predominates over Type 2 inflammation in GA irrespective of histologic pattern. Distinct disease endotypes were not detected.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>38135676</pmid><doi>10.1111/ijd.16998</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-5728-9875</orcidid><orcidid>https://orcid.org/0000-0001-6494-7250</orcidid></addata></record> |
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| source | MEDLINE; Wiley Journals |
| subjects | Adult Aged Biopsy Cytokines Cytokines - metabolism Eosinophils Eosinophils - immunology Eosinophils - pathology Female Granuloma Granuloma Annulare - diagnosis Granuloma Annulare - immunology Granuloma Annulare - pathology Granulomas Heterogeneity Histology Humans Hybridization Immunity In Situ Hybridization Inflammatory diseases Interferon Interferon-gamma Interleukin-17 - metabolism Interleukin-4 Interleukin-5 Leukocytes (eosinophilic) Lymphocytes T Male Middle Aged Retrospective Studies Skin - immunology Skin - pathology Staining Staining and Labeling Th1 Cells - immunology Th2 Cells - immunology Young Adult |
| title | Retrospective assessment of immunologic and histologic heterogeneity in granuloma annulare by cytokine staining |
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