Dual-mode fluorescence and electrochemiluminescence sensors based on Ru-MOF nanosheets for sensitive detection of apoE genes
A fluorescence-electrochemiluminescence (FL-ECL) dual-mode sensor for apoE gene detection has been developed, leveraging the unique properties of ruthenium metal organic framework nanosheets (RuMOFNSs). The system utilizes the quenching effect of the Ru(bpy) 3 2+ ECL signal by ferrocene, leading to...
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| container_title | Journal of materials chemistry. B, Materials for biology and medicine |
| container_volume | 12 |
| creator | Hu, Huiting Cui, Hanfeng Yin, Xia Fan, Qiqi Shuai, Hai Zhang, Jing Liao, Fusheng Xiong, Wei Jiang, Hedong Fan, Hao Liu, Wenming Wei, Guobing |
| description | A fluorescence-electrochemiluminescence (FL-ECL) dual-mode sensor for apoE gene detection has been developed, leveraging the unique properties of ruthenium metal organic framework nanosheets (RuMOFNSs). The system utilizes the quenching effect of the Ru(bpy)
3
2+
ECL signal by ferrocene, leading to the synthesis of a multi-electron electrical signal marker, bisferrocene. By immobilizing the P-DNA on RuMOFNSs, bisferrocene quenches both FL and ECL signals. The addition of T-DNA and the consequent formation of double-stranded DNA enable the ExoIII enzyme to excise the bisferrocene fragment, restoring the signals. The sensor demonstrates wide detection linear ranges (1 fM to 1 nM for FL and 0.01 fM to 10 pM for ECL) and remarkable sensitivity (0.048 fM for FL and 0.016 fM for ECL). The dual-mode design offers enhanced reliability through a self-correction feature, reducing false positives. Compared to single-mode sensors, the dual-mode sensor shows significant advantages. Real-world testing confirms the sensor's capacity for robust detection in actual samples, underscoring its promising application in early disease diagnosis. This innovative approach opens up avenues for multi-signal response sensors, offering significant potential for diagnostic technologies.
A novel FL-ECL dual-mode sensor for apoE gene detection with ruthenium MOF nanosheets exhibits high sensitivity, wide linear ranges, and robust real-world application potential. |
| doi_str_mv | 10.1039/d3tb01934a |
| format | Article |
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3
2+
ECL signal by ferrocene, leading to the synthesis of a multi-electron electrical signal marker, bisferrocene. By immobilizing the P-DNA on RuMOFNSs, bisferrocene quenches both FL and ECL signals. The addition of T-DNA and the consequent formation of double-stranded DNA enable the ExoIII enzyme to excise the bisferrocene fragment, restoring the signals. The sensor demonstrates wide detection linear ranges (1 fM to 1 nM for FL and 0.01 fM to 10 pM for ECL) and remarkable sensitivity (0.048 fM for FL and 0.016 fM for ECL). The dual-mode design offers enhanced reliability through a self-correction feature, reducing false positives. Compared to single-mode sensors, the dual-mode sensor shows significant advantages. Real-world testing confirms the sensor's capacity for robust detection in actual samples, underscoring its promising application in early disease diagnosis. This innovative approach opens up avenues for multi-signal response sensors, offering significant potential for diagnostic technologies.
A novel FL-ECL dual-mode sensor for apoE gene detection with ruthenium MOF nanosheets exhibits high sensitivity, wide linear ranges, and robust real-world application potential.</description><identifier>ISSN: 2050-750X</identifier><identifier>EISSN: 2050-7518</identifier><identifier>DOI: 10.1039/d3tb01934a</identifier><identifier>PMID: 38131524</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Apolipoprotein E ; Deoxyribonucleic acid ; DNA ; Electrochemiluminescence ; Fluorescence ; Metal-organic frameworks ; Nanosheets ; Ruthenium ; Sensors ; T-DNA</subject><ispartof>Journal of materials chemistry. B, Materials for biology and medicine, 2024-01, Vol.12 (3), p.71-79</ispartof><rights>Copyright Royal Society of Chemistry 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-5fc3ec2b54945a81499c9ea39079cdad2d0c8eec043c8a64af3f1903979c1ef63</citedby><cites>FETCH-LOGICAL-c373t-5fc3ec2b54945a81499c9ea39079cdad2d0c8eec043c8a64af3f1903979c1ef63</cites><orcidid>0009-0007-2946-3171 ; 0000-0002-4582-7250 ; 0000-0003-3791-3500</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38131524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Huiting</creatorcontrib><creatorcontrib>Cui, Hanfeng</creatorcontrib><creatorcontrib>Yin, Xia</creatorcontrib><creatorcontrib>Fan, Qiqi</creatorcontrib><creatorcontrib>Shuai, Hai</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Liao, Fusheng</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Jiang, Hedong</creatorcontrib><creatorcontrib>Fan, Hao</creatorcontrib><creatorcontrib>Liu, Wenming</creatorcontrib><creatorcontrib>Wei, Guobing</creatorcontrib><title>Dual-mode fluorescence and electrochemiluminescence sensors based on Ru-MOF nanosheets for sensitive detection of apoE genes</title><title>Journal of materials chemistry. B, Materials for biology and medicine</title><addtitle>J Mater Chem B</addtitle><description>A fluorescence-electrochemiluminescence (FL-ECL) dual-mode sensor for apoE gene detection has been developed, leveraging the unique properties of ruthenium metal organic framework nanosheets (RuMOFNSs). The system utilizes the quenching effect of the Ru(bpy)
3
2+
ECL signal by ferrocene, leading to the synthesis of a multi-electron electrical signal marker, bisferrocene. By immobilizing the P-DNA on RuMOFNSs, bisferrocene quenches both FL and ECL signals. The addition of T-DNA and the consequent formation of double-stranded DNA enable the ExoIII enzyme to excise the bisferrocene fragment, restoring the signals. The sensor demonstrates wide detection linear ranges (1 fM to 1 nM for FL and 0.01 fM to 10 pM for ECL) and remarkable sensitivity (0.048 fM for FL and 0.016 fM for ECL). The dual-mode design offers enhanced reliability through a self-correction feature, reducing false positives. Compared to single-mode sensors, the dual-mode sensor shows significant advantages. Real-world testing confirms the sensor's capacity for robust detection in actual samples, underscoring its promising application in early disease diagnosis. This innovative approach opens up avenues for multi-signal response sensors, offering significant potential for diagnostic technologies.
A novel FL-ECL dual-mode sensor for apoE gene detection with ruthenium MOF nanosheets exhibits high sensitivity, wide linear ranges, and robust real-world application potential.</description><subject>Apolipoprotein E</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Electrochemiluminescence</subject><subject>Fluorescence</subject><subject>Metal-organic frameworks</subject><subject>Nanosheets</subject><subject>Ruthenium</subject><subject>Sensors</subject><subject>T-DNA</subject><issn>2050-750X</issn><issn>2050-7518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpdkVFLwzAUhYMoOqYvvisBX0SoJk3TNY9zcypMBqLgW0mTG-1ok5m0guCPNzqdYF5u4Hwc7j0HoUNKzilh4kKzriJUsExuoUFKOElGnBbbmz952kMHISxJfAXNC5btoj1WUEZ5mg3Qx7SXTdI6Ddg0vfMQFFgFWFqNoQHVeadeoK2bvq3trxjABucDrmQAjZ3F931yt5hhK60LLwBdwMb5b6zu6jfAGrpoVUfSGSxX7go_Q3TbRztGNgEOfuYQPc6uHiY3yXxxfTsZzxPFRqxLuFEMVFrxTGRcFjQTQgmQTJCRUFrqVBNVACiSMVXIPJOGGSpiOFGmYHI2RKdr35V3rz2ErmzreErTSAuuD2UqCOdpzKeI6Mk_dOl6b-N2kaKc5zSPYQ_R2ZpS3oXgwZQrX7fSv5eUlF-1lFP2cPldyzjCxz-WfdWC3qC_JUTgaA34oDbqX6_sE20wks8</recordid><startdate>20240117</startdate><enddate>20240117</enddate><creator>Hu, Huiting</creator><creator>Cui, Hanfeng</creator><creator>Yin, Xia</creator><creator>Fan, Qiqi</creator><creator>Shuai, Hai</creator><creator>Zhang, Jing</creator><creator>Liao, Fusheng</creator><creator>Xiong, Wei</creator><creator>Jiang, Hedong</creator><creator>Fan, Hao</creator><creator>Liu, Wenming</creator><creator>Wei, Guobing</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0007-2946-3171</orcidid><orcidid>https://orcid.org/0000-0002-4582-7250</orcidid><orcidid>https://orcid.org/0000-0003-3791-3500</orcidid></search><sort><creationdate>20240117</creationdate><title>Dual-mode fluorescence and electrochemiluminescence sensors based on Ru-MOF nanosheets for sensitive detection of apoE genes</title><author>Hu, Huiting ; Cui, Hanfeng ; Yin, Xia ; Fan, Qiqi ; Shuai, Hai ; Zhang, Jing ; Liao, Fusheng ; Xiong, Wei ; Jiang, Hedong ; Fan, Hao ; Liu, Wenming ; Wei, Guobing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-5fc3ec2b54945a81499c9ea39079cdad2d0c8eec043c8a64af3f1903979c1ef63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Apolipoprotein E</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Electrochemiluminescence</topic><topic>Fluorescence</topic><topic>Metal-organic frameworks</topic><topic>Nanosheets</topic><topic>Ruthenium</topic><topic>Sensors</topic><topic>T-DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Huiting</creatorcontrib><creatorcontrib>Cui, Hanfeng</creatorcontrib><creatorcontrib>Yin, Xia</creatorcontrib><creatorcontrib>Fan, Qiqi</creatorcontrib><creatorcontrib>Shuai, Hai</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Liao, Fusheng</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Jiang, Hedong</creatorcontrib><creatorcontrib>Fan, Hao</creatorcontrib><creatorcontrib>Liu, Wenming</creatorcontrib><creatorcontrib>Wei, Guobing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Huiting</au><au>Cui, Hanfeng</au><au>Yin, Xia</au><au>Fan, Qiqi</au><au>Shuai, Hai</au><au>Zhang, Jing</au><au>Liao, Fusheng</au><au>Xiong, Wei</au><au>Jiang, Hedong</au><au>Fan, Hao</au><au>Liu, Wenming</au><au>Wei, Guobing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual-mode fluorescence and electrochemiluminescence sensors based on Ru-MOF nanosheets for sensitive detection of apoE genes</atitle><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle><addtitle>J Mater Chem B</addtitle><date>2024-01-17</date><risdate>2024</risdate><volume>12</volume><issue>3</issue><spage>71</spage><epage>79</epage><pages>71-79</pages><issn>2050-750X</issn><eissn>2050-7518</eissn><abstract>A fluorescence-electrochemiluminescence (FL-ECL) dual-mode sensor for apoE gene detection has been developed, leveraging the unique properties of ruthenium metal organic framework nanosheets (RuMOFNSs). The system utilizes the quenching effect of the Ru(bpy)
3
2+
ECL signal by ferrocene, leading to the synthesis of a multi-electron electrical signal marker, bisferrocene. By immobilizing the P-DNA on RuMOFNSs, bisferrocene quenches both FL and ECL signals. The addition of T-DNA and the consequent formation of double-stranded DNA enable the ExoIII enzyme to excise the bisferrocene fragment, restoring the signals. The sensor demonstrates wide detection linear ranges (1 fM to 1 nM for FL and 0.01 fM to 10 pM for ECL) and remarkable sensitivity (0.048 fM for FL and 0.016 fM for ECL). The dual-mode design offers enhanced reliability through a self-correction feature, reducing false positives. Compared to single-mode sensors, the dual-mode sensor shows significant advantages. Real-world testing confirms the sensor's capacity for robust detection in actual samples, underscoring its promising application in early disease diagnosis. This innovative approach opens up avenues for multi-signal response sensors, offering significant potential for diagnostic technologies.
A novel FL-ECL dual-mode sensor for apoE gene detection with ruthenium MOF nanosheets exhibits high sensitivity, wide linear ranges, and robust real-world application potential.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>38131524</pmid><doi>10.1039/d3tb01934a</doi><tpages>9</tpages><orcidid>https://orcid.org/0009-0007-2946-3171</orcidid><orcidid>https://orcid.org/0000-0002-4582-7250</orcidid><orcidid>https://orcid.org/0000-0003-3791-3500</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2050-750X |
| ispartof | Journal of materials chemistry. B, Materials for biology and medicine, 2024-01, Vol.12 (3), p.71-79 |
| issn | 2050-750X 2050-7518 |
| language | eng |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Apolipoprotein E Deoxyribonucleic acid DNA Electrochemiluminescence Fluorescence Metal-organic frameworks Nanosheets Ruthenium Sensors T-DNA |
| title | Dual-mode fluorescence and electrochemiluminescence sensors based on Ru-MOF nanosheets for sensitive detection of apoE genes |
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