Patch-seq: Advances and Biological Applications
Multimodal analysis of gene-expression patterns, electrophysiological properties, and morphological phenotypes at the single-cell/single-nucleus level has been arduous because of the diversity and complexity of neurons. The emergence of Patch-sequencing (Patch-seq) directly links transcriptomics, mo...
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| Veröffentlicht in: | Cellular and molecular neurobiology 2024-12, Vol.44 (1), p.8, Article 8 |
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| container_title | Cellular and molecular neurobiology |
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| creator | Shao, Mingting Zhang, Wei Li, Ye Tang, Lei Hao, Zhao-Zhe Liu, Sheng |
| description | Multimodal analysis of gene-expression patterns, electrophysiological properties, and morphological phenotypes at the single-cell/single-nucleus level has been arduous because of the diversity and complexity of neurons. The emergence of Patch-sequencing (Patch-seq) directly links transcriptomics, morphology, and electrophysiology, taking neuroscience research to a multimodal era. In this review, we summarized the development of Patch-seq and recent applications in the cortex, hippocampus, and other nervous systems. Through generating multimodal cell type atlases, targeting specific cell populations, and correlating transcriptomic data with phenotypic information, Patch-seq has provided new insight into outstanding questions in neuroscience. We highlight the challenges and opportunities of Patch-seq in neuroscience and hope to shed new light on future neuroscience research. |
| doi_str_mv | 10.1007/s10571-023-01436-3 |
| format | Article |
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| subjects | Biomedical and Life Sciences Biomedicine Cell Biology Electrophysiology Gene expression Gene Expression Profiling Nervous system Neurobiology Neurosciences Patch-Clamp Techniques Phenotypes Review Paper Sequence Analysis, RNA Single-Cell Analysis Transcriptome Transcriptomics |
| title | Patch-seq: Advances and Biological Applications |
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