Protein Dot Stamp Using Hydrophobin as Immobilization Carrier

Protein Dot Stamp Using Hydrophobin as Immobilization Carrier

Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labele...

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Veröffentlicht in:Sensors and materials 2004-01, Vol.16 (8), p.413-420
Hauptverfasser: Ikeno, Shinya, Szilvay, Geza R, Linder, Markus, Aritomi, Hijiri, Ajimi, Masako, Haruyama, Tetsuya
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container_end_page 420
container_issue 8
container_start_page 413
container_title Sensors and materials
container_volume 16
creator Ikeno, Shinya
Szilvay, Geza R
Linder, Markus
Aritomi, Hijiri
Ajimi, Masako
Haruyama, Tetsuya
description Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.
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title Protein Dot Stamp Using Hydrophobin as Immobilization Carrier
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