Fluorescent characterization of differentiated myotubes using flow cytometry

Flow cytometry is routinely used in the assessment of skeletal muscle progenitor cell (myoblast) populations. However, a full gating strategy, inclusive of difficult to interpret forward and side scatter data, which documents cytometric analysis of differentiated myoblasts (myotubes) has not been re...

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Veröffentlicht in:	Cytometry. Part A 2024-05, Vol.105 (5), p.332-344
Hauptverfasser:	Nolan, Andy, Heaton, Robert A., Adamova, Petra, Cole, Paige, Turton, Nadia, Gillham, Scott H., Owens, Daniel J., Sexton, Darren W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Annexin V Apoptosis C2C12 Cell morphology Cell suspensions Cell viability Cells (biology) Clusters Cytology Deoxyribonucleic acid DNA DNA probes Flow Flow cytometry Fluorescence Fluorescent indicators Heterogeneity Iodides Labeling Laser beams Lasers Metabolism Microscopy Mitochondria Mitochondrial DNA Morphology Myoblasts Myosin Myotubes Populations Progenitor cells Propidium iodide Reactive oxygen species Scattering Size determination Skeletal muscle Surface properties
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