Distinguishing Molecular Mechanical Action from Photothermal and Photodynamic Behavior

Molecular motors (MM) are molecular machines, or nanomachines, that rotate unidirectionally upon photostimulation and perform mechanical work on their environment. In the last several years, it has been shown that the photomechanical action of MM can be used to permeabilize lipid bilayers, thereby killing cancer cells and pathogenic microorganisms and controlling cell signaling. The work contributes to a growing acknowledgement that the molecular actuation characteristic of these systems is useful for various applications in biology. However, the mechanical effects of molecular motion on biological materials are difficult to disentangle from photodynamic and photothermal action, which are also present when a light‐absorbing fluorophore is irradiated with light. Here, an overview of the key methods used by various research groups to distinguish the effects of photomechanical, photodynamic, and photothermal action is provided. It is anticipated that this discussion will be helpful to the community seeking to use MM to develop new and distinctive medical technologies that result from mechanical disruption of biological materials. Molecular motors are small molecule actuators that can permeabilize lipid bilayers, kill cancer cells and microorganisms, and control cell signaling. Here, a roadmap is provided detailing how to distinguish the photomechanical action characteristic to these motors from photothermal and photodynamic effects. It is hoped that this framework leads to a robust criterion for the isolation of the effects of molecular motion as the community seeks to use molecular motors for new and distinctive applications.