Endogenous Extracellular Vesicles Participate in Brain Remodeling after Ischemic Stroke
Brain remodeling after an ischemic stroke represents a promising avenue for exploring the cellular mechanisms of endogenous brain repair. A deeper understanding of these mechanisms is crucial for optimizing the safety and efficacy of neuroprotective treatments for stroke patients. Here, we interroga...
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| Veröffentlicht in: | International journal of molecular sciences 2023-12, Vol.24 (23), p.16857 |
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| container_title | International journal of molecular sciences |
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| creator | Alvarez, Mauricio Muleiro Salazar, Felipe Esparza Rodriguez, Thomas D'Egidio, Francesco Borlongan, Cesar V Lee, Jea-Young |
| description | Brain remodeling after an ischemic stroke represents a promising avenue for exploring the cellular mechanisms of endogenous brain repair. A deeper understanding of these mechanisms is crucial for optimizing the safety and efficacy of neuroprotective treatments for stroke patients. Here, we interrogated the role of extracellular vesicles, particularly exosomes, as potential mediators of endogenous repair within the neurovascular unit (NVU). We hypothesized that these extracellular vesicles may play a role in achieving transient stroke neuroprotection. Using the established ischemic stroke model of middle cerebral artery occlusion in adult rats, we detected a surged in the extracellular vesicle marker CD63 in the peri-infarct area that either juxtaposed or co-localized with GFAP-positive glial cells, MAP2-labeled young neurons, and VEGF-marked angiogenic cells. This novel observation that CD63 exosomes spatially and temporally approximated glial activation, neurogenesis, and angiogenesis suggests that extracellular vesicles, especially exosomes, contribute to the endogenous repair of the NVU, warranting exploration of extracellular vesicle-based stroke therapeutics. |
| doi_str_mv | 10.3390/ijms242316857 |
| format | Article |
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A deeper understanding of these mechanisms is crucial for optimizing the safety and efficacy of neuroprotective treatments for stroke patients. Here, we interrogated the role of extracellular vesicles, particularly exosomes, as potential mediators of endogenous repair within the neurovascular unit (NVU). We hypothesized that these extracellular vesicles may play a role in achieving transient stroke neuroprotection. Using the established ischemic stroke model of middle cerebral artery occlusion in adult rats, we detected a surged in the extracellular vesicle marker CD63 in the peri-infarct area that either juxtaposed or co-localized with GFAP-positive glial cells, MAP2-labeled young neurons, and VEGF-marked angiogenic cells. 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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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| subjects | Angiogenesis Animals Atherosclerosis Blood platelets Brain Brain Ischemia Cell cycle Cell death Cytokines Extracellular Vesicles Homeopathy Humans Infarction, Middle Cerebral Artery Ischemia Ischemic Stroke Materia medica and therapeutics Neurogenesis Neurons Rats Stem cells Stroke Stroke (Disease) Stroke patients Therapeutics Vascular endothelial growth factor |
| title | Endogenous Extracellular Vesicles Participate in Brain Remodeling after Ischemic Stroke |
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