Anti-Biofilm Activity of Cocultimycin A against Candida albicans
( ), the most common fungal pathogen, has the ability to form a biofilm, leading to enhanced virulence and antibiotic resistance. Cocultimycin A, a novel antifungal antibiotic isolated from the co-culture of two marine fungi, exhibited a potent inhibitory effect on planktonic cells. This study aimed...
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| Veröffentlicht in: | International journal of molecular sciences 2023-12, Vol.24 (23), p.17026 |
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| creator | Zhu, Xiaohong Wang, Anqi Zheng, Yifan Li, Dan Wei, Yuanjuan Gan, Maoluo Li, Yan Si, Shuyi |
| description | (
), the most common fungal pathogen, has the ability to form a biofilm, leading to enhanced virulence and antibiotic resistance. Cocultimycin A, a novel antifungal antibiotic isolated from the co-culture of two marine fungi, exhibited a potent inhibitory effect on planktonic
cells. This study aimed to evaluate the anti-biofilm activity of cocultimycin A against
and explore its underlying mechanism. Crystal violet staining showed that cocultimycin A remarkably inhibited biofilm formation in a dose-dependent manner and disrupted mature biofilms at higher concentrations. However, the metabolic activity of mature biofilms treated with lower concentrations of cocultimycin A significantly decreased when using the XTT reduction method. Cocultimycin A could inhibit yeast-to-hypha transition and mycelium formation of
colonies, which was observed through the use of a light microscope. Scanning electron microscopy revealed that biofilms treated with cocultimycin A were disrupted, yeast cells increased, and hypha cells decreased and significantly shortened. The adhesive ability of
cells treated with cocultimycin A to the medium and HOEC cells significantly decreased. Through the use of a qRT-PCR assay, the expression of multiple genes related to adhesion, hyphal formation and cell membrane changes in relation to biofilm cells treated with cocultimycin A. All these results suggested that cocultimycin A may be considered a potential novel molecule for treating and preventing biofilm-related
infections. |
| doi_str_mv | 10.3390/ijms242317026 |
| format | Article |
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), the most common fungal pathogen, has the ability to form a biofilm, leading to enhanced virulence and antibiotic resistance. Cocultimycin A, a novel antifungal antibiotic isolated from the co-culture of two marine fungi, exhibited a potent inhibitory effect on planktonic
cells. This study aimed to evaluate the anti-biofilm activity of cocultimycin A against
and explore its underlying mechanism. Crystal violet staining showed that cocultimycin A remarkably inhibited biofilm formation in a dose-dependent manner and disrupted mature biofilms at higher concentrations. However, the metabolic activity of mature biofilms treated with lower concentrations of cocultimycin A significantly decreased when using the XTT reduction method. Cocultimycin A could inhibit yeast-to-hypha transition and mycelium formation of
colonies, which was observed through the use of a light microscope. Scanning electron microscopy revealed that biofilms treated with cocultimycin A were disrupted, yeast cells increased, and hypha cells decreased and significantly shortened. The adhesive ability of
cells treated with cocultimycin A to the medium and HOEC cells significantly decreased. Through the use of a qRT-PCR assay, the expression of multiple genes related to adhesion, hyphal formation and cell membrane changes in relation to biofilm cells treated with cocultimycin A. All these results suggested that cocultimycin A may be considered a potential novel molecule for treating and preventing biofilm-related
infections.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms242317026</identifier><identifier>PMID: 38069349</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antifungal agents ; Antifungal Agents - chemistry ; Antifungal Agents - pharmacology ; Biofilms ; Candida albicans ; Candidiasis - microbiology ; Clinical medicine ; Drug dosages ; Fungal infections ; Gentian Violet - pharmacology ; Metabolism ; Natural products ; Virulence ; Yeast</subject><ispartof>International journal of molecular sciences, 2023-12, Vol.24 (23), p.17026</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-9778c844032c239553ad93a22667b1d27fa1a38e5b71f1aa9c160a503bfd6cc3</citedby><cites>FETCH-LOGICAL-c360t-9778c844032c239553ad93a22667b1d27fa1a38e5b71f1aa9c160a503bfd6cc3</cites><orcidid>0000-0002-3089-8654</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38069349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Xiaohong</creatorcontrib><creatorcontrib>Wang, Anqi</creatorcontrib><creatorcontrib>Zheng, Yifan</creatorcontrib><creatorcontrib>Li, Dan</creatorcontrib><creatorcontrib>Wei, Yuanjuan</creatorcontrib><creatorcontrib>Gan, Maoluo</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Si, Shuyi</creatorcontrib><title>Anti-Biofilm Activity of Cocultimycin A against Candida albicans</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>(
), the most common fungal pathogen, has the ability to form a biofilm, leading to enhanced virulence and antibiotic resistance. Cocultimycin A, a novel antifungal antibiotic isolated from the co-culture of two marine fungi, exhibited a potent inhibitory effect on planktonic
cells. This study aimed to evaluate the anti-biofilm activity of cocultimycin A against
and explore its underlying mechanism. Crystal violet staining showed that cocultimycin A remarkably inhibited biofilm formation in a dose-dependent manner and disrupted mature biofilms at higher concentrations. However, the metabolic activity of mature biofilms treated with lower concentrations of cocultimycin A significantly decreased when using the XTT reduction method. Cocultimycin A could inhibit yeast-to-hypha transition and mycelium formation of
colonies, which was observed through the use of a light microscope. Scanning electron microscopy revealed that biofilms treated with cocultimycin A were disrupted, yeast cells increased, and hypha cells decreased and significantly shortened. The adhesive ability of
cells treated with cocultimycin A to the medium and HOEC cells significantly decreased. Through the use of a qRT-PCR assay, the expression of multiple genes related to adhesion, hyphal formation and cell membrane changes in relation to biofilm cells treated with cocultimycin A. All these results suggested that cocultimycin A may be considered a potential novel molecule for treating and preventing biofilm-related
infections.</description><subject>Antifungal agents</subject><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - pharmacology</subject><subject>Biofilms</subject><subject>Candida albicans</subject><subject>Candidiasis - microbiology</subject><subject>Clinical medicine</subject><subject>Drug dosages</subject><subject>Fungal infections</subject><subject>Gentian Violet - pharmacology</subject><subject>Metabolism</subject><subject>Natural products</subject><subject>Virulence</subject><subject>Yeast</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpd0E1Lw0AQBuBFFFurR68S8OIlOjuT7GZvxuIXFLz0HiabRLbko2YTof_eSFVUGJg5PLwMrxDnEq6JDNy4TeMxQpIaUB2IuYwQQwClD3_dM3Hi_QYACWNzLGaUgDIUmbm4TdvBhXeuq1zdBKkd3LsbdkFXBcvOjvXgmp11bZAG_Mqu9UOw5LZwBQdc585y60_FUcW1L8--9kKsH-7Xy6dw9fL4vExXoSUFQ2i0TmwSRUBokUwcExeGGFEpncsCdcWSKSnjXMtKMhsrFXAMlFeFspYW4mofu-27t7H0Q9Y4b8u65rbsRp-hATRqGpro5T-66ca-nZ7LMDEmoigGPalwr2zfed-XVbbtXcP9LpOQfTab_Wl28hdfqWPelMWP_q6SPgBN6XH6</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Zhu, Xiaohong</creator><creator>Wang, Anqi</creator><creator>Zheng, Yifan</creator><creator>Li, Dan</creator><creator>Wei, Yuanjuan</creator><creator>Gan, Maoluo</creator><creator>Li, Yan</creator><creator>Si, Shuyi</creator><general>MDPI AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3089-8654</orcidid></search><sort><creationdate>20231201</creationdate><title>Anti-Biofilm Activity of Cocultimycin A against Candida albicans</title><author>Zhu, Xiaohong ; 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), the most common fungal pathogen, has the ability to form a biofilm, leading to enhanced virulence and antibiotic resistance. Cocultimycin A, a novel antifungal antibiotic isolated from the co-culture of two marine fungi, exhibited a potent inhibitory effect on planktonic
cells. This study aimed to evaluate the anti-biofilm activity of cocultimycin A against
and explore its underlying mechanism. Crystal violet staining showed that cocultimycin A remarkably inhibited biofilm formation in a dose-dependent manner and disrupted mature biofilms at higher concentrations. However, the metabolic activity of mature biofilms treated with lower concentrations of cocultimycin A significantly decreased when using the XTT reduction method. Cocultimycin A could inhibit yeast-to-hypha transition and mycelium formation of
colonies, which was observed through the use of a light microscope. Scanning electron microscopy revealed that biofilms treated with cocultimycin A were disrupted, yeast cells increased, and hypha cells decreased and significantly shortened. The adhesive ability of
cells treated with cocultimycin A to the medium and HOEC cells significantly decreased. Through the use of a qRT-PCR assay, the expression of multiple genes related to adhesion, hyphal formation and cell membrane changes in relation to biofilm cells treated with cocultimycin A. All these results suggested that cocultimycin A may be considered a potential novel molecule for treating and preventing biofilm-related
infections.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38069349</pmid><doi>10.3390/ijms242317026</doi><orcidid>https://orcid.org/0000-0002-3089-8654</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Antifungal agents Antifungal Agents - chemistry Antifungal Agents - pharmacology Biofilms Candida albicans Candidiasis - microbiology Clinical medicine Drug dosages Fungal infections Gentian Violet - pharmacology Metabolism Natural products Virulence Yeast |
| title | Anti-Biofilm Activity of Cocultimycin A against Candida albicans |
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