Combining fungal bioremediation and ozonation for rinse wastewater treatment

In this work, agricultural rinse wastewater, which is produced during the cleaning of agricultural equipment and constitutes a major source of pesticides, was treated by fungal bioremediation and ozonation, both individually and combined in a two-stage treatment train. Three major pesticides (thiacloprid, chlortoluron, and pyrimethanil) were detected in rinse wastewater, with a total concentration of 38.47 mg C L−1. Comparing both technologies, ozonation in a stirred reactor achieved complete removal of these pesticides (720 min) while proving to be a more effective approach for reducing colour, organic matter, and bacteria. However, this technique produced transformation products and increased toxicity. In contrast, fungal bioremediation in a rotating drum bioreactor attenuated toxicity levels and did not produce such metabolites, but only removed approximately 50 % of target pesticide - hydraulic retention time (HRT) of 5 days - and obtained worse results for most of the general quality parameters studied. This work also includes a preliminary economic assessment of both technologies, revealing that fungal bioremediation was 2 times more cost-effective than ozonation. The treatment train, consisting of a first stage of fungal bioremediation followed by ozonation, was found to be a promising approach as it synergistically combines the advantages of both treatments, achieving high removals of pesticides (up to 100 %) and transformation products, while reducing operating costs and producing a biodegradable effluent. This is the first time that fungal bioremediation and ozonation technologies have been compared and combined in a treatment train to deal with pesticides in agricultural rinse wastewater. [Display omitted] •Agricultural rinse wastewater was successfully treated by different technologies.•Fungal bioremediation was better in terms of toxicity, formation of TPs and cost.•Ozonation was superior in removing pesticides, colour, organic matter and bacteria.•The treatment train integrated the advantages of both techniques.