Use of Fourier-transform infrared spectroscopy for real-time outbreak investigation of OXA-48-producing Escherichia coli

Abstract Background Efficient infection control during carbapenem-resistant Enterobacterales outbreaks demands rapid and simple techniques for outbreak investigations. WGS, the current gold standard for outbreak identification, is expensive, time-consuming and requires a high level of expertise. Fou...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of antimicrobial chemotherapy 2024-02, Vol.79 (2), p.349-353
Hauptverfasser: Kon, Hadas, Lurie-Weinberger, Mor N, Lugassy, Carmela, Chen, Dafna, Schechner, Vered, Schwaber, Mitchell J, Hussein, Khetam, Alon, Tamar, Tarabeia, Jalal, Hamo, Moran, Firan, Ibraheem, Aboalhega, Worood, Lomansov, Elena, Mendelsohn, Sigal, Keren-Paz, Alona, Carmeli, Yehuda
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 353
container_issue 2
container_start_page 349
container_title Journal of antimicrobial chemotherapy
container_volume 79
creator Kon, Hadas
Lurie-Weinberger, Mor N
Lugassy, Carmela
Chen, Dafna
Schechner, Vered
Schwaber, Mitchell J
Hussein, Khetam
Alon, Tamar
Tarabeia, Jalal
Hamo, Moran
Firan, Ibraheem
Aboalhega, Worood
Lomansov, Elena
Mendelsohn, Sigal
Keren-Paz, Alona
Carmeli, Yehuda
description Abstract Background Efficient infection control during carbapenem-resistant Enterobacterales outbreaks demands rapid and simple techniques for outbreak investigations. WGS, the current gold standard for outbreak identification, is expensive, time-consuming and requires a high level of expertise. Fourier-transform infrared (FTIR) spectroscopy (IR Biotyper) is a rapid typing method based on infrared radiation applied to samples, which provides a highly specific absorption spectrum. Objectives To investigate an outbreak of OXA-48-producing Escherichia coli in real-time using FTIR and subsequently compare the results with WGS. Methods Twenty-one isolates were collected during a nosocomial outbreak, and identification and antibiotic susceptibilities were confirmed by VITEK®2. FTIR was conducted for all isolates, and nine representative isolates were sequenced. Results FTIR was able to correctly determine the clonal relatedness of the isolates and to identify the outbreak cluster, as confirmed by WGS. By WGS, isolates in the main FTIR cluster belonged to the same MLST type and core-genome MLST type, and they harboured similar plasmids and resistance genes, whereas the singletons external to the FTIR cluster had different genetic content. Conclusions FTIR can operate as a rapid, efficient and reliable first-line tool for outbreak investigations during a real-time ongoing E. coli outbreak, which can contribute to limiting the spread of pathogens.
doi_str_mv 10.1093/jac/dkad387
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2902948595</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/jac/dkad387</oup_id><sourcerecordid>2902948595</sourcerecordid><originalsourceid>FETCH-LOGICAL-c278t-5c08dc43b49c6807a009932f1a5129a090e55c7a9b03a93ef87d3f38b7b41173</originalsourceid><addsrcrecordid>eNp9kMFLwzAUh4Mobk5P3qUnEST60iRrcpSxqSB4UfBW0jTdsrVNTVpx_70Zmx49PR7vex_v_RC6JHBHQNL7tdL35UaVVGRHaEzYFHAKkhyjMVDgOGOcjtBZCGsAmPKpOEUjKggQydgYfb8Hk7gqWbjBW-Nx71UbKuebxLaVV96USeiM7r0L2nXbJI4Sb1SNe9vExaEvYreJ8JcJvV2q3rp253v9eMBM4M67ctC2XSbzoFfGW72yKtGutufopFJ1MBeHOkFvi_nb7Am_vD4-zx5esE4z0WOuQZSa0YJJPRWQKQApaVoRxUkqFUgwnOtMyQKoktRUIitpRUWRFYyQjE7QzV4bL_kc4o15Y4M2da1a44aQpxJSyQSXPKK3e1THZ4M3Vd552yi_zQnku6TzmHR-SDrSVwfxUDSm_GN_o43A9R5wQ_ev6QcR_4iR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2902948595</pqid></control><display><type>article</type><title>Use of Fourier-transform infrared spectroscopy for real-time outbreak investigation of OXA-48-producing Escherichia coli</title><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Kon, Hadas ; Lurie-Weinberger, Mor N ; Lugassy, Carmela ; Chen, Dafna ; Schechner, Vered ; Schwaber, Mitchell J ; Hussein, Khetam ; Alon, Tamar ; Tarabeia, Jalal ; Hamo, Moran ; Firan, Ibraheem ; Aboalhega, Worood ; Lomansov, Elena ; Mendelsohn, Sigal ; Keren-Paz, Alona ; Carmeli, Yehuda</creator><creatorcontrib>Kon, Hadas ; Lurie-Weinberger, Mor N ; Lugassy, Carmela ; Chen, Dafna ; Schechner, Vered ; Schwaber, Mitchell J ; Hussein, Khetam ; Alon, Tamar ; Tarabeia, Jalal ; Hamo, Moran ; Firan, Ibraheem ; Aboalhega, Worood ; Lomansov, Elena ; Mendelsohn, Sigal ; Keren-Paz, Alona ; Carmeli, Yehuda</creatorcontrib><description>Abstract Background Efficient infection control during carbapenem-resistant Enterobacterales outbreaks demands rapid and simple techniques for outbreak investigations. WGS, the current gold standard for outbreak identification, is expensive, time-consuming and requires a high level of expertise. Fourier-transform infrared (FTIR) spectroscopy (IR Biotyper) is a rapid typing method based on infrared radiation applied to samples, which provides a highly specific absorption spectrum. Objectives To investigate an outbreak of OXA-48-producing Escherichia coli in real-time using FTIR and subsequently compare the results with WGS. Methods Twenty-one isolates were collected during a nosocomial outbreak, and identification and antibiotic susceptibilities were confirmed by VITEK®2. FTIR was conducted for all isolates, and nine representative isolates were sequenced. Results FTIR was able to correctly determine the clonal relatedness of the isolates and to identify the outbreak cluster, as confirmed by WGS. By WGS, isolates in the main FTIR cluster belonged to the same MLST type and core-genome MLST type, and they harboured similar plasmids and resistance genes, whereas the singletons external to the FTIR cluster had different genetic content. Conclusions FTIR can operate as a rapid, efficient and reliable first-line tool for outbreak investigations during a real-time ongoing E. coli outbreak, which can contribute to limiting the spread of pathogens.</description><identifier>ISSN: 0305-7453</identifier><identifier>ISSN: 1460-2091</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkad387</identifier><identifier>PMID: 38101944</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><ispartof>Journal of antimicrobial chemotherapy, 2024-02, Vol.79 (2), p.349-353</ispartof><rights>The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2023</rights><rights>The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c278t-5c08dc43b49c6807a009932f1a5129a090e55c7a9b03a93ef87d3f38b7b41173</cites><orcidid>0000-0002-0679-3967</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38101944$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kon, Hadas</creatorcontrib><creatorcontrib>Lurie-Weinberger, Mor N</creatorcontrib><creatorcontrib>Lugassy, Carmela</creatorcontrib><creatorcontrib>Chen, Dafna</creatorcontrib><creatorcontrib>Schechner, Vered</creatorcontrib><creatorcontrib>Schwaber, Mitchell J</creatorcontrib><creatorcontrib>Hussein, Khetam</creatorcontrib><creatorcontrib>Alon, Tamar</creatorcontrib><creatorcontrib>Tarabeia, Jalal</creatorcontrib><creatorcontrib>Hamo, Moran</creatorcontrib><creatorcontrib>Firan, Ibraheem</creatorcontrib><creatorcontrib>Aboalhega, Worood</creatorcontrib><creatorcontrib>Lomansov, Elena</creatorcontrib><creatorcontrib>Mendelsohn, Sigal</creatorcontrib><creatorcontrib>Keren-Paz, Alona</creatorcontrib><creatorcontrib>Carmeli, Yehuda</creatorcontrib><title>Use of Fourier-transform infrared spectroscopy for real-time outbreak investigation of OXA-48-producing Escherichia coli</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Abstract Background Efficient infection control during carbapenem-resistant Enterobacterales outbreaks demands rapid and simple techniques for outbreak investigations. WGS, the current gold standard for outbreak identification, is expensive, time-consuming and requires a high level of expertise. Fourier-transform infrared (FTIR) spectroscopy (IR Biotyper) is a rapid typing method based on infrared radiation applied to samples, which provides a highly specific absorption spectrum. Objectives To investigate an outbreak of OXA-48-producing Escherichia coli in real-time using FTIR and subsequently compare the results with WGS. Methods Twenty-one isolates were collected during a nosocomial outbreak, and identification and antibiotic susceptibilities were confirmed by VITEK®2. FTIR was conducted for all isolates, and nine representative isolates were sequenced. Results FTIR was able to correctly determine the clonal relatedness of the isolates and to identify the outbreak cluster, as confirmed by WGS. By WGS, isolates in the main FTIR cluster belonged to the same MLST type and core-genome MLST type, and they harboured similar plasmids and resistance genes, whereas the singletons external to the FTIR cluster had different genetic content. Conclusions FTIR can operate as a rapid, efficient and reliable first-line tool for outbreak investigations during a real-time ongoing E. coli outbreak, which can contribute to limiting the spread of pathogens.</description><issn>0305-7453</issn><issn>1460-2091</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kMFLwzAUh4Mobk5P3qUnEST60iRrcpSxqSB4UfBW0jTdsrVNTVpx_70Zmx49PR7vex_v_RC6JHBHQNL7tdL35UaVVGRHaEzYFHAKkhyjMVDgOGOcjtBZCGsAmPKpOEUjKggQydgYfb8Hk7gqWbjBW-Nx71UbKuebxLaVV96USeiM7r0L2nXbJI4Sb1SNe9vExaEvYreJ8JcJvV2q3rp253v9eMBM4M67ctC2XSbzoFfGW72yKtGutufopFJ1MBeHOkFvi_nb7Am_vD4-zx5esE4z0WOuQZSa0YJJPRWQKQApaVoRxUkqFUgwnOtMyQKoktRUIitpRUWRFYyQjE7QzV4bL_kc4o15Y4M2da1a44aQpxJSyQSXPKK3e1THZ4M3Vd552yi_zQnku6TzmHR-SDrSVwfxUDSm_GN_o43A9R5wQ_ev6QcR_4iR</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Kon, Hadas</creator><creator>Lurie-Weinberger, Mor N</creator><creator>Lugassy, Carmela</creator><creator>Chen, Dafna</creator><creator>Schechner, Vered</creator><creator>Schwaber, Mitchell J</creator><creator>Hussein, Khetam</creator><creator>Alon, Tamar</creator><creator>Tarabeia, Jalal</creator><creator>Hamo, Moran</creator><creator>Firan, Ibraheem</creator><creator>Aboalhega, Worood</creator><creator>Lomansov, Elena</creator><creator>Mendelsohn, Sigal</creator><creator>Keren-Paz, Alona</creator><creator>Carmeli, Yehuda</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0679-3967</orcidid></search><sort><creationdate>20240201</creationdate><title>Use of Fourier-transform infrared spectroscopy for real-time outbreak investigation of OXA-48-producing Escherichia coli</title><author>Kon, Hadas ; Lurie-Weinberger, Mor N ; Lugassy, Carmela ; Chen, Dafna ; Schechner, Vered ; Schwaber, Mitchell J ; Hussein, Khetam ; Alon, Tamar ; Tarabeia, Jalal ; Hamo, Moran ; Firan, Ibraheem ; Aboalhega, Worood ; Lomansov, Elena ; Mendelsohn, Sigal ; Keren-Paz, Alona ; Carmeli, Yehuda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c278t-5c08dc43b49c6807a009932f1a5129a090e55c7a9b03a93ef87d3f38b7b41173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kon, Hadas</creatorcontrib><creatorcontrib>Lurie-Weinberger, Mor N</creatorcontrib><creatorcontrib>Lugassy, Carmela</creatorcontrib><creatorcontrib>Chen, Dafna</creatorcontrib><creatorcontrib>Schechner, Vered</creatorcontrib><creatorcontrib>Schwaber, Mitchell J</creatorcontrib><creatorcontrib>Hussein, Khetam</creatorcontrib><creatorcontrib>Alon, Tamar</creatorcontrib><creatorcontrib>Tarabeia, Jalal</creatorcontrib><creatorcontrib>Hamo, Moran</creatorcontrib><creatorcontrib>Firan, Ibraheem</creatorcontrib><creatorcontrib>Aboalhega, Worood</creatorcontrib><creatorcontrib>Lomansov, Elena</creatorcontrib><creatorcontrib>Mendelsohn, Sigal</creatorcontrib><creatorcontrib>Keren-Paz, Alona</creatorcontrib><creatorcontrib>Carmeli, Yehuda</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kon, Hadas</au><au>Lurie-Weinberger, Mor N</au><au>Lugassy, Carmela</au><au>Chen, Dafna</au><au>Schechner, Vered</au><au>Schwaber, Mitchell J</au><au>Hussein, Khetam</au><au>Alon, Tamar</au><au>Tarabeia, Jalal</au><au>Hamo, Moran</au><au>Firan, Ibraheem</au><au>Aboalhega, Worood</au><au>Lomansov, Elena</au><au>Mendelsohn, Sigal</au><au>Keren-Paz, Alona</au><au>Carmeli, Yehuda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of Fourier-transform infrared spectroscopy for real-time outbreak investigation of OXA-48-producing Escherichia coli</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2024-02-01</date><risdate>2024</risdate><volume>79</volume><issue>2</issue><spage>349</spage><epage>353</epage><pages>349-353</pages><issn>0305-7453</issn><issn>1460-2091</issn><eissn>1460-2091</eissn><abstract>Abstract Background Efficient infection control during carbapenem-resistant Enterobacterales outbreaks demands rapid and simple techniques for outbreak investigations. WGS, the current gold standard for outbreak identification, is expensive, time-consuming and requires a high level of expertise. Fourier-transform infrared (FTIR) spectroscopy (IR Biotyper) is a rapid typing method based on infrared radiation applied to samples, which provides a highly specific absorption spectrum. Objectives To investigate an outbreak of OXA-48-producing Escherichia coli in real-time using FTIR and subsequently compare the results with WGS. Methods Twenty-one isolates were collected during a nosocomial outbreak, and identification and antibiotic susceptibilities were confirmed by VITEK®2. FTIR was conducted for all isolates, and nine representative isolates were sequenced. Results FTIR was able to correctly determine the clonal relatedness of the isolates and to identify the outbreak cluster, as confirmed by WGS. By WGS, isolates in the main FTIR cluster belonged to the same MLST type and core-genome MLST type, and they harboured similar plasmids and resistance genes, whereas the singletons external to the FTIR cluster had different genetic content. Conclusions FTIR can operate as a rapid, efficient and reliable first-line tool for outbreak investigations during a real-time ongoing E. coli outbreak, which can contribute to limiting the spread of pathogens.</abstract><cop>US</cop><pub>Oxford University Press</pub><pmid>38101944</pmid><doi>10.1093/jac/dkad387</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-0679-3967</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0305-7453
ispartof Journal of antimicrobial chemotherapy, 2024-02, Vol.79 (2), p.349-353
issn 0305-7453
1460-2091
1460-2091
language eng
recordid cdi_proquest_miscellaneous_2902948595
source Oxford University Press Journals All Titles (1996-Current)
title Use of Fourier-transform infrared spectroscopy for real-time outbreak investigation of OXA-48-producing Escherichia coli
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T16%3A58%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Use%20of%20Fourier-transform%20infrared%20spectroscopy%20for%20real-time%20outbreak%20investigation%20of%20OXA-48-producing%20Escherichia%20coli&rft.jtitle=Journal%20of%20antimicrobial%20chemotherapy&rft.au=Kon,%20Hadas&rft.date=2024-02-01&rft.volume=79&rft.issue=2&rft.spage=349&rft.epage=353&rft.pages=349-353&rft.issn=0305-7453&rft.eissn=1460-2091&rft_id=info:doi/10.1093/jac/dkad387&rft_dat=%3Cproquest_cross%3E2902948595%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2902948595&rft_id=info:pmid/38101944&rft_oup_id=10.1093/jac/dkad387&rfr_iscdi=true