Targeted Transcriptional Activation Using a CRISPR-Associated Transposon System

Targeted Transcriptional Activation Using a CRISPR-Associated Transposon System

Synthetic perturbation of gene expression is central to our ability to reliably uncover genotype–phenotype relationships in microbes. Here, we present a novel transcription activation strategy that uses the Vibrio cholerae CRISPR-Associated Transposon (CAST) system to selectively insert promoter ele...

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Veröffentlicht in:ACS synthetic biology 2024-01, Vol.13 (1), p.328-336
Hauptverfasser: Garza Elizondo, Andrea M., Chappell, James
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Chappell, James
description Synthetic perturbation of gene expression is central to our ability to reliably uncover genotype–phenotype relationships in microbes. Here, we present a novel transcription activation strategy that uses the Vibrio cholerae CRISPR-Associated Transposon (CAST) system to selectively insert promoter elements upstream of genes of interest. Through this strategy, we show robust activation of both recombinant and endogenous genes across the Escherichia coli chromosome. We then demonstrate the precise tuning of expression levels by exchanging the promoter elements being inserted. Finally, we demonstrate that CAST activation can be used to synthetically induce ampicillin-resistant phenotypes in E. coli.
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title Targeted Transcriptional Activation Using a CRISPR-Associated Transposon System
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