Regenerative Potential of Granulocyte Colony-Stimulating Factor Immobilized by Using Electron-Beam Synthesis Nanotechnology in an Experimental Model of Ovarian Reserve Depletion
The pharmacological activity of granulocyte CSF (G-CSF) immobilized using electron-beam synthesis nanotechnology (imG-CSF) was evaluated in an experimental model of ovarian reserve depletion. The effectiveness of the drug was compared with that of its unmodified form. Depletion of the ovarian follic...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2023-11, Vol.176 (1), p.101-104 |
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| creator | Borovskaya, T. G. Vychuzhanina, A. V. Ligacheva, A. A. Shchemerova, Yu.A. Sandrikina, L. A. Madonov, P. G. Rakitin, F. A. Goldberg, V. E. Dygai, A. M. |
| description | The pharmacological activity of granulocyte CSF (G-CSF) immobilized using electron-beam synthesis nanotechnology (imG-CSF) was evaluated in an experimental model of ovarian reserve depletion. The effectiveness of the drug was compared with that of its unmodified form. Depletion of the ovarian follicular pool in female Sprague-Dawley rats was caused by a single intravenous injection of the antitumor drug etoposide in the maximum tolerated dose. The effectiveness of the studied drugs was assessed by serum concentration of anti-Mullerian hormone (AMH) measured by ELISA and by the number of primordial, two-layer, multilayer, and atretic follicles counted on serial sections of the ovaries (5-μm thick; through the entire organ) stained with hematoxylin and eosin. It was found that imG-CSF prevents depletion of the ovarian reserve in the model used, which was confirmed by high AMH concentration and higher numbers of primordial, two- and multilayer follicles in comparison with the corresponding parameters in the control (etoposide), and by a decrease in the severity of atretic processes. Unmodified form of the drug demonstrated lower efficiency. |
| doi_str_mv | 10.1007/s10517-023-05975-z |
| format | Article |
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G. ; Vychuzhanina, A. V. ; Ligacheva, A. A. ; Shchemerova, Yu.A. ; Sandrikina, L. A. ; Madonov, P. G. ; Rakitin, F. A. ; Goldberg, V. E. ; Dygai, A. M.</creator><creatorcontrib>Borovskaya, T. G. ; Vychuzhanina, A. V. ; Ligacheva, A. A. ; Shchemerova, Yu.A. ; Sandrikina, L. A. ; Madonov, P. G. ; Rakitin, F. A. ; Goldberg, V. E. ; Dygai, A. M.</creatorcontrib><description>The pharmacological activity of granulocyte CSF (G-CSF) immobilized using electron-beam synthesis nanotechnology (imG-CSF) was evaluated in an experimental model of ovarian reserve depletion. The effectiveness of the drug was compared with that of its unmodified form. Depletion of the ovarian follicular pool in female Sprague-Dawley rats was caused by a single intravenous injection of the antitumor drug etoposide in the maximum tolerated dose. The effectiveness of the studied drugs was assessed by serum concentration of anti-Mullerian hormone (AMH) measured by ELISA and by the number of primordial, two-layer, multilayer, and atretic follicles counted on serial sections of the ovaries (5-μm thick; through the entire organ) stained with hematoxylin and eosin. It was found that imG-CSF prevents depletion of the ovarian reserve in the model used, which was confirmed by high AMH concentration and higher numbers of primordial, two- and multilayer follicles in comparison with the corresponding parameters in the control (etoposide), and by a decrease in the severity of atretic processes. Unmodified form of the drug demonstrated lower efficiency.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-023-05975-z</identifier><identifier>PMID: 38085397</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Anti-Mullerian Hormone ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Colony-stimulating factor ; Comparative analysis ; Electrons ; Etoposide ; Female ; Follicles ; Granulocyte colony-stimulating factor ; Granulocyte Colony-Stimulating Factor - pharmacology ; Granulocytes ; Internal Medicine ; Laboratory Medicine ; Leukocytes (granulocytic) ; Models, Theoretical ; Nanotechnology ; Ovarian Reserve ; Ovaries ; Pathology ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Bulletin of experimental biology and medicine, 2023-11, Vol.176 (1), p.101-104</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2023. 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Depletion of the ovarian follicular pool in female Sprague-Dawley rats was caused by a single intravenous injection of the antitumor drug etoposide in the maximum tolerated dose. The effectiveness of the studied drugs was assessed by serum concentration of anti-Mullerian hormone (AMH) measured by ELISA and by the number of primordial, two-layer, multilayer, and atretic follicles counted on serial sections of the ovaries (5-μm thick; through the entire organ) stained with hematoxylin and eosin. It was found that imG-CSF prevents depletion of the ovarian reserve in the model used, which was confirmed by high AMH concentration and higher numbers of primordial, two- and multilayer follicles in comparison with the corresponding parameters in the control (etoposide), and by a decrease in the severity of atretic processes. 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G.</au><au>Vychuzhanina, A. V.</au><au>Ligacheva, A. A.</au><au>Shchemerova, Yu.A.</au><au>Sandrikina, L. A.</au><au>Madonov, P. G.</au><au>Rakitin, F. A.</au><au>Goldberg, V. E.</au><au>Dygai, A. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regenerative Potential of Granulocyte Colony-Stimulating Factor Immobilized by Using Electron-Beam Synthesis Nanotechnology in an Experimental Model of Ovarian Reserve Depletion</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2023-11-01</date><risdate>2023</risdate><volume>176</volume><issue>1</issue><spage>101</spage><epage>104</epage><pages>101-104</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><abstract>The pharmacological activity of granulocyte CSF (G-CSF) immobilized using electron-beam synthesis nanotechnology (imG-CSF) was evaluated in an experimental model of ovarian reserve depletion. The effectiveness of the drug was compared with that of its unmodified form. Depletion of the ovarian follicular pool in female Sprague-Dawley rats was caused by a single intravenous injection of the antitumor drug etoposide in the maximum tolerated dose. The effectiveness of the studied drugs was assessed by serum concentration of anti-Mullerian hormone (AMH) measured by ELISA and by the number of primordial, two-layer, multilayer, and atretic follicles counted on serial sections of the ovaries (5-μm thick; through the entire organ) stained with hematoxylin and eosin. It was found that imG-CSF prevents depletion of the ovarian reserve in the model used, which was confirmed by high AMH concentration and higher numbers of primordial, two- and multilayer follicles in comparison with the corresponding parameters in the control (etoposide), and by a decrease in the severity of atretic processes. Unmodified form of the drug demonstrated lower efficiency.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38085397</pmid><doi>10.1007/s10517-023-05975-z</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Anti-Mullerian Hormone Biomedical and Life Sciences Biomedicine Cell Biology Colony-stimulating factor Comparative analysis Electrons Etoposide Female Follicles Granulocyte colony-stimulating factor Granulocyte Colony-Stimulating Factor - pharmacology Granulocytes Internal Medicine Laboratory Medicine Leukocytes (granulocytic) Models, Theoretical Nanotechnology Ovarian Reserve Ovaries Pathology Rats Rats, Sprague-Dawley |
| title | Regenerative Potential of Granulocyte Colony-Stimulating Factor Immobilized by Using Electron-Beam Synthesis Nanotechnology in an Experimental Model of Ovarian Reserve Depletion |
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