The Effects of ROCK Inhibitor on Prevention of Dexamethasone-Induced Glaucoma Phenotype in Human Trabecular Meshwork Cells
This study investigated the effects of dexamethasone (Dex) on human trabecular meshwork (TM) cells, a model of glucocorticoid-induced glaucoma, and evaluated the impact of ripasudil (Rip) as a co-delivery or sequential dosing strategy. In vitro experiments were conducted to assess the effects of Dex...
Gespeichert in:
Veröffentlicht in: | Translational vision science & technology 2023-12, Vol.12 (12), p.4-4 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 4 |
---|---|
container_issue | 12 |
container_start_page | 4 |
container_title | Translational vision science & technology |
container_volume | 12 |
creator | Debele, Tilahun Ayane Mount, Zachary F Yuan, Yong Kao, Winston W-Y Park, Yoonjee C |
description | This study investigated the effects of dexamethasone (Dex) on human trabecular meshwork (TM) cells, a model of glucocorticoid-induced glaucoma, and evaluated the impact of ripasudil (Rip) as a co-delivery or sequential dosing strategy.
In vitro experiments were conducted to assess the effects of Dex and Rip on TM cells. Confocal microscopy was used to evaluate the impact of Dex and Rip on F-actin staining signals. Contractility of the TM cells upon Dex and Rip treatment mimicking co-delivery and sequential delivery was quantified using collagen gel contraction assay. Transepithelial electrical resistance (TEER) values and fluorescein isothiocyanate (FITC)-dextran permeability were also measured to assess the impact of Dex and Rip on TM cells.
Dex and Rip did not exhibit cytotoxicity at the maximum tested concentration (20 µM). Dex-treated TM cells exhibited higher F-actin staining signals compared to controls, which were reduced when co-treated with Rip. Rip inhibited Dex-induced collagen gel contraction activity in both co-delivery and sequential treatments. Dex resulted in increased TEER values as the dose increased, whereas TEER values were maintained when co-treated with Rip.
Co-delivery of Rip has the potential to prevent glaucoma symptoms when patients are treated with Dex. This study highlights the importance of identifying strategies to reduce the side effects of prolonged use of glucocorticoids, such as Dex, in the treatment of various diseases.
This study demonstrates the potential of co-delivering ripasudil with dexamethasone to mitigate glucocorticoid-induced ocular hypertension and a secondary glaucoma that resembles primary open-angle glaucoma, providing insights for the development of novel preventive strategies in clinical care. |
doi_str_mv | 10.1167/tvst.12.12.4 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2898955324</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2898955324</sourcerecordid><originalsourceid>FETCH-LOGICAL-c286t-d373433da98684d122fb5d695092cba7b468f736d58d8f246c219e6749b740623</originalsourceid><addsrcrecordid>eNpNkEtLxDAUhYMoKurOtWTpwo7No2m6lPExg4oi47qkyS2ttsmYpL5-vS0-8HLgHrgfh8tB6JCkM0JEfhpfQ5wROolvoF1KBE9oVpDNf34HHYTwlI4jZMa52EY7TKYZoSLfRZ-rBvBFXYOOAbsaP9zNr_HSNm3VRuexs_jewyvY2I52vJ_Du-ohNio4C8nSmkGDwVedGrTrFb5vwLr4sQbcWrwYemXxyqsK9NApj28hNG_OP-M5dF3YR1u16gIc_Ow99Hh5sZovkpu7q-X87CbRVIqYGJYzzphRhRSSG0JpXWVGFFlaUF2pvOJC1jkTJpNG1pQLTUkBIudFlfNUULaHjr9z1969DBBi2bdBjx8oC24IJZWFLLKMUT6iJ9-o9i4ED3W59m2v_EdJ0nIqvJwKLwmdNOFHP8lD1YP5g3_rZV9Tm3wB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2898955324</pqid></control><display><type>article</type><title>The Effects of ROCK Inhibitor on Prevention of Dexamethasone-Induced Glaucoma Phenotype in Human Trabecular Meshwork Cells</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Debele, Tilahun Ayane ; Mount, Zachary F ; Yuan, Yong ; Kao, Winston W-Y ; Park, Yoonjee C</creator><creatorcontrib>Debele, Tilahun Ayane ; Mount, Zachary F ; Yuan, Yong ; Kao, Winston W-Y ; Park, Yoonjee C</creatorcontrib><description>This study investigated the effects of dexamethasone (Dex) on human trabecular meshwork (TM) cells, a model of glucocorticoid-induced glaucoma, and evaluated the impact of ripasudil (Rip) as a co-delivery or sequential dosing strategy.
In vitro experiments were conducted to assess the effects of Dex and Rip on TM cells. Confocal microscopy was used to evaluate the impact of Dex and Rip on F-actin staining signals. Contractility of the TM cells upon Dex and Rip treatment mimicking co-delivery and sequential delivery was quantified using collagen gel contraction assay. Transepithelial electrical resistance (TEER) values and fluorescein isothiocyanate (FITC)-dextran permeability were also measured to assess the impact of Dex and Rip on TM cells.
Dex and Rip did not exhibit cytotoxicity at the maximum tested concentration (20 µM). Dex-treated TM cells exhibited higher F-actin staining signals compared to controls, which were reduced when co-treated with Rip. Rip inhibited Dex-induced collagen gel contraction activity in both co-delivery and sequential treatments. Dex resulted in increased TEER values as the dose increased, whereas TEER values were maintained when co-treated with Rip.
Co-delivery of Rip has the potential to prevent glaucoma symptoms when patients are treated with Dex. This study highlights the importance of identifying strategies to reduce the side effects of prolonged use of glucocorticoids, such as Dex, in the treatment of various diseases.
This study demonstrates the potential of co-delivering ripasudil with dexamethasone to mitigate glucocorticoid-induced ocular hypertension and a secondary glaucoma that resembles primary open-angle glaucoma, providing insights for the development of novel preventive strategies in clinical care.</description><identifier>ISSN: 2164-2591</identifier><identifier>EISSN: 2164-2591</identifier><identifier>DOI: 10.1167/tvst.12.12.4</identifier><identifier>PMID: 38051267</identifier><language>eng</language><publisher>United States</publisher><subject>Actins - pharmacology ; Collagen ; Dexamethasone - toxicity ; Glaucoma - chemically induced ; Glaucoma - drug therapy ; Glaucoma - prevention & control ; Glaucoma, Open-Angle ; Glucocorticoids - adverse effects ; Humans ; Phenotype ; rho-Associated Kinases - pharmacology ; Trabecular Meshwork</subject><ispartof>Translational vision science & technology, 2023-12, Vol.12 (12), p.4-4</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c286t-d373433da98684d122fb5d695092cba7b468f736d58d8f246c219e6749b740623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38051267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Debele, Tilahun Ayane</creatorcontrib><creatorcontrib>Mount, Zachary F</creatorcontrib><creatorcontrib>Yuan, Yong</creatorcontrib><creatorcontrib>Kao, Winston W-Y</creatorcontrib><creatorcontrib>Park, Yoonjee C</creatorcontrib><title>The Effects of ROCK Inhibitor on Prevention of Dexamethasone-Induced Glaucoma Phenotype in Human Trabecular Meshwork Cells</title><title>Translational vision science & technology</title><addtitle>Transl Vis Sci Technol</addtitle><description>This study investigated the effects of dexamethasone (Dex) on human trabecular meshwork (TM) cells, a model of glucocorticoid-induced glaucoma, and evaluated the impact of ripasudil (Rip) as a co-delivery or sequential dosing strategy.
In vitro experiments were conducted to assess the effects of Dex and Rip on TM cells. Confocal microscopy was used to evaluate the impact of Dex and Rip on F-actin staining signals. Contractility of the TM cells upon Dex and Rip treatment mimicking co-delivery and sequential delivery was quantified using collagen gel contraction assay. Transepithelial electrical resistance (TEER) values and fluorescein isothiocyanate (FITC)-dextran permeability were also measured to assess the impact of Dex and Rip on TM cells.
Dex and Rip did not exhibit cytotoxicity at the maximum tested concentration (20 µM). Dex-treated TM cells exhibited higher F-actin staining signals compared to controls, which were reduced when co-treated with Rip. Rip inhibited Dex-induced collagen gel contraction activity in both co-delivery and sequential treatments. Dex resulted in increased TEER values as the dose increased, whereas TEER values were maintained when co-treated with Rip.
Co-delivery of Rip has the potential to prevent glaucoma symptoms when patients are treated with Dex. This study highlights the importance of identifying strategies to reduce the side effects of prolonged use of glucocorticoids, such as Dex, in the treatment of various diseases.
This study demonstrates the potential of co-delivering ripasudil with dexamethasone to mitigate glucocorticoid-induced ocular hypertension and a secondary glaucoma that resembles primary open-angle glaucoma, providing insights for the development of novel preventive strategies in clinical care.</description><subject>Actins - pharmacology</subject><subject>Collagen</subject><subject>Dexamethasone - toxicity</subject><subject>Glaucoma - chemically induced</subject><subject>Glaucoma - drug therapy</subject><subject>Glaucoma - prevention & control</subject><subject>Glaucoma, Open-Angle</subject><subject>Glucocorticoids - adverse effects</subject><subject>Humans</subject><subject>Phenotype</subject><subject>rho-Associated Kinases - pharmacology</subject><subject>Trabecular Meshwork</subject><issn>2164-2591</issn><issn>2164-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtLxDAUhYMoKurOtWTpwo7No2m6lPExg4oi47qkyS2ttsmYpL5-vS0-8HLgHrgfh8tB6JCkM0JEfhpfQ5wROolvoF1KBE9oVpDNf34HHYTwlI4jZMa52EY7TKYZoSLfRZ-rBvBFXYOOAbsaP9zNr_HSNm3VRuexs_jewyvY2I52vJ_Du-ohNio4C8nSmkGDwVedGrTrFb5vwLr4sQbcWrwYemXxyqsK9NApj28hNG_OP-M5dF3YR1u16gIc_Ow99Hh5sZovkpu7q-X87CbRVIqYGJYzzphRhRSSG0JpXWVGFFlaUF2pvOJC1jkTJpNG1pQLTUkBIudFlfNUULaHjr9z1969DBBi2bdBjx8oC24IJZWFLLKMUT6iJ9-o9i4ED3W59m2v_EdJ0nIqvJwKLwmdNOFHP8lD1YP5g3_rZV9Tm3wB</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Debele, Tilahun Ayane</creator><creator>Mount, Zachary F</creator><creator>Yuan, Yong</creator><creator>Kao, Winston W-Y</creator><creator>Park, Yoonjee C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20231201</creationdate><title>The Effects of ROCK Inhibitor on Prevention of Dexamethasone-Induced Glaucoma Phenotype in Human Trabecular Meshwork Cells</title><author>Debele, Tilahun Ayane ; Mount, Zachary F ; Yuan, Yong ; Kao, Winston W-Y ; Park, Yoonjee C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c286t-d373433da98684d122fb5d695092cba7b468f736d58d8f246c219e6749b740623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Actins - pharmacology</topic><topic>Collagen</topic><topic>Dexamethasone - toxicity</topic><topic>Glaucoma - chemically induced</topic><topic>Glaucoma - drug therapy</topic><topic>Glaucoma - prevention & control</topic><topic>Glaucoma, Open-Angle</topic><topic>Glucocorticoids - adverse effects</topic><topic>Humans</topic><topic>Phenotype</topic><topic>rho-Associated Kinases - pharmacology</topic><topic>Trabecular Meshwork</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Debele, Tilahun Ayane</creatorcontrib><creatorcontrib>Mount, Zachary F</creatorcontrib><creatorcontrib>Yuan, Yong</creatorcontrib><creatorcontrib>Kao, Winston W-Y</creatorcontrib><creatorcontrib>Park, Yoonjee C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Translational vision science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Debele, Tilahun Ayane</au><au>Mount, Zachary F</au><au>Yuan, Yong</au><au>Kao, Winston W-Y</au><au>Park, Yoonjee C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effects of ROCK Inhibitor on Prevention of Dexamethasone-Induced Glaucoma Phenotype in Human Trabecular Meshwork Cells</atitle><jtitle>Translational vision science & technology</jtitle><addtitle>Transl Vis Sci Technol</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>12</volume><issue>12</issue><spage>4</spage><epage>4</epage><pages>4-4</pages><issn>2164-2591</issn><eissn>2164-2591</eissn><abstract>This study investigated the effects of dexamethasone (Dex) on human trabecular meshwork (TM) cells, a model of glucocorticoid-induced glaucoma, and evaluated the impact of ripasudil (Rip) as a co-delivery or sequential dosing strategy.
In vitro experiments were conducted to assess the effects of Dex and Rip on TM cells. Confocal microscopy was used to evaluate the impact of Dex and Rip on F-actin staining signals. Contractility of the TM cells upon Dex and Rip treatment mimicking co-delivery and sequential delivery was quantified using collagen gel contraction assay. Transepithelial electrical resistance (TEER) values and fluorescein isothiocyanate (FITC)-dextran permeability were also measured to assess the impact of Dex and Rip on TM cells.
Dex and Rip did not exhibit cytotoxicity at the maximum tested concentration (20 µM). Dex-treated TM cells exhibited higher F-actin staining signals compared to controls, which were reduced when co-treated with Rip. Rip inhibited Dex-induced collagen gel contraction activity in both co-delivery and sequential treatments. Dex resulted in increased TEER values as the dose increased, whereas TEER values were maintained when co-treated with Rip.
Co-delivery of Rip has the potential to prevent glaucoma symptoms when patients are treated with Dex. This study highlights the importance of identifying strategies to reduce the side effects of prolonged use of glucocorticoids, such as Dex, in the treatment of various diseases.
This study demonstrates the potential of co-delivering ripasudil with dexamethasone to mitigate glucocorticoid-induced ocular hypertension and a secondary glaucoma that resembles primary open-angle glaucoma, providing insights for the development of novel preventive strategies in clinical care.</abstract><cop>United States</cop><pmid>38051267</pmid><doi>10.1167/tvst.12.12.4</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2164-2591 |
ispartof | Translational vision science & technology, 2023-12, Vol.12 (12), p.4-4 |
issn | 2164-2591 2164-2591 |
language | eng |
recordid | cdi_proquest_miscellaneous_2898955324 |
source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
subjects | Actins - pharmacology Collagen Dexamethasone - toxicity Glaucoma - chemically induced Glaucoma - drug therapy Glaucoma - prevention & control Glaucoma, Open-Angle Glucocorticoids - adverse effects Humans Phenotype rho-Associated Kinases - pharmacology Trabecular Meshwork |
title | The Effects of ROCK Inhibitor on Prevention of Dexamethasone-Induced Glaucoma Phenotype in Human Trabecular Meshwork Cells |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T22%3A33%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Effects%20of%20ROCK%20Inhibitor%20on%20Prevention%20of%20Dexamethasone-Induced%20Glaucoma%20Phenotype%20in%20Human%20Trabecular%20Meshwork%20Cells&rft.jtitle=Translational%20vision%20science%20&%20technology&rft.au=Debele,%20Tilahun%20Ayane&rft.date=2023-12-01&rft.volume=12&rft.issue=12&rft.spage=4&rft.epage=4&rft.pages=4-4&rft.issn=2164-2591&rft.eissn=2164-2591&rft_id=info:doi/10.1167/tvst.12.12.4&rft_dat=%3Cproquest_cross%3E2898955324%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2898955324&rft_id=info:pmid/38051267&rfr_iscdi=true |