An Ex Vivo Aorta Culture Model to Study Vascular Cellular Senescence

Animal studies on vascular aging pose a few limitations. One of the most important reasons for this is the absence of a fast and efficient model of vascular tissue aging. In this study, ex vivo aortic culture and Matrigel subcutaneous implantation are combined to develop a new model for studying vas...

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Veröffentlicht in:Advanced biology 2024-03, Vol.8 (3), p.e2300140-n/a
Hauptverfasser: Yu, Yijie, Bian, Shihui, Jiang, Yu, Li, Bo, Cui, Xinggang, Ding, Shu, Dai, Zhiyin, Chen, Rui, Zhong, Wei, Yuan, Wei
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container_issue 3
container_start_page e2300140
container_title Advanced biology
container_volume 8
creator Yu, Yijie
Bian, Shihui
Jiang, Yu
Li, Bo
Cui, Xinggang
Ding, Shu
Dai, Zhiyin
Chen, Rui
Zhong, Wei
Yuan, Wei
description Animal studies on vascular aging pose a few limitations. One of the most important reasons for this is the absence of a fast and efficient model of vascular tissue aging. In this study, ex vivo aortic culture and Matrigel subcutaneous implantation are combined to develop a new model for studying vascular cellular senescence. Eight‐week‐old C57BL/6J mice are used to obtain aortas. Bleomycin is used to induce aortas senescence in vitro. Then, aortas are transplanted to the acceptor mice with Matrigel. Senescence is evaluated using western blotting, quantitative polymerase chain reaction, and senescence‐associated beta‐galactosidase activity. Inflammatory cytokines are detected using Luminex Liquid Suspension Chip. RNA levels are analyzed by transcriptome sequencing. The results revealed that vessels in the bleomycin group exhibited significant senescence than those in the control group that can be enhanced by stripping vessel adventitia. The levels of cytokines such as interleukin (IL‐2, IL‐1β, and IL‐6 increased significantly in the ex vivo model. Furthermore, transcriptome sequencing revealed 56 significantly differentially expressed genes (DEGs) in ex vivo model vessels compared with those in naturally aging aortas. In conclusion, this study introduces a cost‐effective and time‐saving vessel senescence model for vascular cellular senescence. This article introduces a cost‐effective and time‐saving vascular cellular senescence model that through treating mice aortas with bleomycin in vitro and then transplanting the treated vessels to the acceptor mice with Matrigel. The researchers verified the efficiency of their model and compared it with the nature aging mice vessels. This model serves as an alternative for vascular aging studies.
doi_str_mv 10.1002/adbi.202300140
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One of the most important reasons for this is the absence of a fast and efficient model of vascular tissue aging. In this study, ex vivo aortic culture and Matrigel subcutaneous implantation are combined to develop a new model for studying vascular cellular senescence. Eight‐week‐old C57BL/6J mice are used to obtain aortas. Bleomycin is used to induce aortas senescence in vitro. Then, aortas are transplanted to the acceptor mice with Matrigel. Senescence is evaluated using western blotting, quantitative polymerase chain reaction, and senescence‐associated beta‐galactosidase activity. Inflammatory cytokines are detected using Luminex Liquid Suspension Chip. RNA levels are analyzed by transcriptome sequencing. The results revealed that vessels in the bleomycin group exhibited significant senescence than those in the control group that can be enhanced by stripping vessel adventitia. The levels of cytokines such as interleukin (IL‐2, IL‐1β, and IL‐6 increased significantly in the ex vivo model. Furthermore, transcriptome sequencing revealed 56 significantly differentially expressed genes (DEGs) in ex vivo model vessels compared with those in naturally aging aortas. In conclusion, this study introduces a cost‐effective and time‐saving vessel senescence model for vascular cellular senescence. This article introduces a cost‐effective and time‐saving vascular cellular senescence model that through treating mice aortas with bleomycin in vitro and then transplanting the treated vessels to the acceptor mice with Matrigel. The researchers verified the efficiency of their model and compared it with the nature aging mice vessels. 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One of the most important reasons for this is the absence of a fast and efficient model of vascular tissue aging. In this study, ex vivo aortic culture and Matrigel subcutaneous implantation are combined to develop a new model for studying vascular cellular senescence. Eight‐week‐old C57BL/6J mice are used to obtain aortas. Bleomycin is used to induce aortas senescence in vitro. Then, aortas are transplanted to the acceptor mice with Matrigel. Senescence is evaluated using western blotting, quantitative polymerase chain reaction, and senescence‐associated beta‐galactosidase activity. Inflammatory cytokines are detected using Luminex Liquid Suspension Chip. RNA levels are analyzed by transcriptome sequencing. The results revealed that vessels in the bleomycin group exhibited significant senescence than those in the control group that can be enhanced by stripping vessel adventitia. The levels of cytokines such as interleukin (IL‐2, IL‐1β, and IL‐6 increased significantly in the ex vivo model. Furthermore, transcriptome sequencing revealed 56 significantly differentially expressed genes (DEGs) in ex vivo model vessels compared with those in naturally aging aortas. In conclusion, this study introduces a cost‐effective and time‐saving vessel senescence model for vascular cellular senescence. This article introduces a cost‐effective and time‐saving vascular cellular senescence model that through treating mice aortas with bleomycin in vitro and then transplanting the treated vessels to the acceptor mice with Matrigel. The researchers verified the efficiency of their model and compared it with the nature aging mice vessels. 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subjects Animals
Aorta
Bleomycin
Cellular Senescence - genetics
Cytokines
ex vivo
Mice
Mice, Inbred C57BL
senescence model
vascular aging
vessel culture
title An Ex Vivo Aorta Culture Model to Study Vascular Cellular Senescence
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