Dynamic Gut Microbiota of Apolygus lucorum Across Different Life Stages Reveals Potential Pathogenic Bacteria for Facilitating the Pest Management
Insect’s gut microbiota has diverse effects on their fitness, and a comprehensive understanding of gut microbiota functions requires analyzing its diversity. Apolygus lucorum is a highly destructive pest that threatens many economically important crops in China. This study investigated the gut micro...
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| Veröffentlicht in: | Microbial ecology 2024-12, Vol.87 (1), p.9-9, Article 9 |
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| creator | Guo, Danni Ge, Jingfan Tang, Zhenzhen Tian, Baoku Li, Wanning Li, Chong Xu, Letian Luo, Jing |
| description | Insect’s gut microbiota has diverse effects on their fitness, and a comprehensive understanding of gut microbiota functions requires analyzing its diversity.
Apolygus lucorum
is a highly destructive pest that threatens many economically important crops in China. This study investigated the gut microbiota of
A. lucorum
across its life cycle using both culture-dependent and culture-independent methods. A total of 87 gut bacterial isolates were identified, belonging to 4 phyla, 27 families, and 45 genera, while Miseq sequencing detected 91 amplicon sequence variants (ASVs) assigned to 5 phyla, 28 families, and 39 genera. Proteobacteria and Firmicutes were the predominant phyla, with
Staphylococcus
and
Serratia
as the major genera. There were significant differences in the relative abundance of these genera between the nymph and adult stages.
Staphylococcus
was significantly more abundant in nymphs than it in adults, while
Serratia
was significantly more abundant in sexually mature adults than in other developmental stages. Notably,
Serratia
is a common opportunistic pathogen in many insects. Injecting the gut-dominant isolate
Serratia marcescens
verified its high pathogenicity. Additionally, immune indicators of the bug at different developmental stages supported the hypothesis that
Serratia
is a pathogen of
A. lucorum
. This study provides a foundation for understanding the role of gut bacteria in the life history of
A. lucorum
and developing new pest control strategies based on microbes. |
| doi_str_mv | 10.1007/s00248-023-02324-5 |
| format | Article |
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Apolygus lucorum
is a highly destructive pest that threatens many economically important crops in China. This study investigated the gut microbiota of
A. lucorum
across its life cycle using both culture-dependent and culture-independent methods. A total of 87 gut bacterial isolates were identified, belonging to 4 phyla, 27 families, and 45 genera, while Miseq sequencing detected 91 amplicon sequence variants (ASVs) assigned to 5 phyla, 28 families, and 39 genera. Proteobacteria and Firmicutes were the predominant phyla, with
Staphylococcus
and
Serratia
as the major genera. There were significant differences in the relative abundance of these genera between the nymph and adult stages.
Staphylococcus
was significantly more abundant in nymphs than it in adults, while
Serratia
was significantly more abundant in sexually mature adults than in other developmental stages. Notably,
Serratia
is a common opportunistic pathogen in many insects. Injecting the gut-dominant isolate
Serratia marcescens
verified its high pathogenicity. Additionally, immune indicators of the bug at different developmental stages supported the hypothesis that
Serratia
is a pathogen of
A. lucorum
. This study provides a foundation for understanding the role of gut bacteria in the life history of
A. lucorum
and developing new pest control strategies based on microbes.</description><identifier>ISSN: 0095-3628</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s00248-023-02324-5</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adults ; Apolygus lucorum ; Bacteria ; Biomedical and Life Sciences ; China ; Culture ; Developmental stages ; digestive system ; Ecology ; Economic importance ; Geoecology/Natural Processes ; Insects ; Intestinal flora ; Intestinal microflora ; intestinal microorganisms ; Life cycle ; Life cycles ; Life history ; Life Sciences ; Microbial Ecology ; Microbiology ; Microbiota ; Microorganisms ; Nature Conservation ; Nymphs ; Opportunist infection ; opportunistic pathogens ; Pathogenic bacteria ; Pathogenicity ; Pathogens ; Pest control ; pest management ; Pests ; Relative abundance ; Serratia ; Serratia marcescens ; sexual maturity ; Staphylococcus ; Water Quality/Water Pollution</subject><ispartof>Microbial ecology, 2024-12, Vol.87 (1), p.9-9, Article 9</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c336t-925f63fc6bebb1fb35d1f114164a999293177e2266c08bf571bfd978dc1d35813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00248-023-02324-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://doi.org/10.1007/s00248-023-02324-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41099,41467,42168,42536,51298,51555</link.rule.ids><linktorsrc>$$Uhttps://doi.org/10.1007/s00248-023-02324-5$$EView_record_in_Springer_Nature$$FView_record_in_$$GSpringer_Nature</linktorsrc></links><search><creatorcontrib>Guo, Danni</creatorcontrib><creatorcontrib>Ge, Jingfan</creatorcontrib><creatorcontrib>Tang, Zhenzhen</creatorcontrib><creatorcontrib>Tian, Baoku</creatorcontrib><creatorcontrib>Li, Wanning</creatorcontrib><creatorcontrib>Li, Chong</creatorcontrib><creatorcontrib>Xu, Letian</creatorcontrib><creatorcontrib>Luo, Jing</creatorcontrib><title>Dynamic Gut Microbiota of Apolygus lucorum Across Different Life Stages Reveals Potential Pathogenic Bacteria for Facilitating the Pest Management</title><title>Microbial ecology</title><addtitle>Microb Ecol</addtitle><description>Insect’s gut microbiota has diverse effects on their fitness, and a comprehensive understanding of gut microbiota functions requires analyzing its diversity.
Apolygus lucorum
is a highly destructive pest that threatens many economically important crops in China. This study investigated the gut microbiota of
A. lucorum
across its life cycle using both culture-dependent and culture-independent methods. A total of 87 gut bacterial isolates were identified, belonging to 4 phyla, 27 families, and 45 genera, while Miseq sequencing detected 91 amplicon sequence variants (ASVs) assigned to 5 phyla, 28 families, and 39 genera. Proteobacteria and Firmicutes were the predominant phyla, with
Staphylococcus
and
Serratia
as the major genera. There were significant differences in the relative abundance of these genera between the nymph and adult stages.
Staphylococcus
was significantly more abundant in nymphs than it in adults, while
Serratia
was significantly more abundant in sexually mature adults than in other developmental stages. Notably,
Serratia
is a common opportunistic pathogen in many insects. Injecting the gut-dominant isolate
Serratia marcescens
verified its high pathogenicity. Additionally, immune indicators of the bug at different developmental stages supported the hypothesis that
Serratia
is a pathogen of
A. lucorum
. This study provides a foundation for understanding the role of gut bacteria in the life history of
A. lucorum
and developing new pest control strategies based on microbes.</description><subject>Adults</subject><subject>Apolygus lucorum</subject><subject>Bacteria</subject><subject>Biomedical and Life Sciences</subject><subject>China</subject><subject>Culture</subject><subject>Developmental stages</subject><subject>digestive system</subject><subject>Ecology</subject><subject>Economic importance</subject><subject>Geoecology/Natural Processes</subject><subject>Insects</subject><subject>Intestinal flora</subject><subject>Intestinal microflora</subject><subject>intestinal microorganisms</subject><subject>Life cycle</subject><subject>Life cycles</subject><subject>Life history</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Nature Conservation</subject><subject>Nymphs</subject><subject>Opportunist infection</subject><subject>opportunistic pathogens</subject><subject>Pathogenic bacteria</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Pest control</subject><subject>pest management</subject><subject>Pests</subject><subject>Relative abundance</subject><subject>Serratia</subject><subject>Serratia marcescens</subject><subject>sexual maturity</subject><subject>Staphylococcus</subject><subject>Water Quality/Water Pollution</subject><issn>0095-3628</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkcuO0zAUhi0EEmXgBVhZYsMmjO0TO8myzDDDSEVUXCR2luMeZzxK4mI7SH0Nnhi3RUJiAQvLi_P9ny8_IS85e8MZay4TY6JuKybguERdyUdkxWsQFW_rb4_JirFOVqBE-5Q8S-mBMd4oASvy8_owm8lbertk-sHbGHofsqHB0fU-jIdhSXRcbIjLRNdlmhK99s5hxDnTjXdIP2czYKKf8AeaMdFtyGXkzUi3Jt-HAecif2tsxugNdSHSG2P96LPJfh5ovke6xVTONnPxTCX7nDxxxYQvfu8X5OvNuy9X76vNx9u7q_WmsgAqV52QToGzqse-564HueOO85qr2nRdJzrgTYNCKGVZ2zvZ8N7tuqbdWb4D2XK4IK_P3n0M35dyBz35ZHEczYxhSRq4BKkAQPwXFW3X1K1i4oi--gt9CEucy0NOlBSSgSyUOFOnL43o9D76ycSD5kwfG9XnRnVpU58a1ccQnEOpwPOA8Y_6H6lfT06kQw</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Guo, Danni</creator><creator>Ge, Jingfan</creator><creator>Tang, Zhenzhen</creator><creator>Tian, Baoku</creator><creator>Li, Wanning</creator><creator>Li, Chong</creator><creator>Xu, Letian</creator><creator>Luo, Jing</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20241201</creationdate><title>Dynamic Gut Microbiota of Apolygus lucorum Across Different Life Stages Reveals Potential Pathogenic Bacteria for Facilitating the Pest Management</title><author>Guo, Danni ; 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Apolygus lucorum
is a highly destructive pest that threatens many economically important crops in China. This study investigated the gut microbiota of
A. lucorum
across its life cycle using both culture-dependent and culture-independent methods. A total of 87 gut bacterial isolates were identified, belonging to 4 phyla, 27 families, and 45 genera, while Miseq sequencing detected 91 amplicon sequence variants (ASVs) assigned to 5 phyla, 28 families, and 39 genera. Proteobacteria and Firmicutes were the predominant phyla, with
Staphylococcus
and
Serratia
as the major genera. There were significant differences in the relative abundance of these genera between the nymph and adult stages.
Staphylococcus
was significantly more abundant in nymphs than it in adults, while
Serratia
was significantly more abundant in sexually mature adults than in other developmental stages. Notably,
Serratia
is a common opportunistic pathogen in many insects. Injecting the gut-dominant isolate
Serratia marcescens
verified its high pathogenicity. Additionally, immune indicators of the bug at different developmental stages supported the hypothesis that
Serratia
is a pathogen of
A. lucorum
. This study provides a foundation for understanding the role of gut bacteria in the life history of
A. lucorum
and developing new pest control strategies based on microbes.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s00248-023-02324-5</doi><tpages>1</tpages></addata></record> |
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| subjects | Adults Apolygus lucorum Bacteria Biomedical and Life Sciences China Culture Developmental stages digestive system Ecology Economic importance Geoecology/Natural Processes Insects Intestinal flora Intestinal microflora intestinal microorganisms Life cycle Life cycles Life history Life Sciences Microbial Ecology Microbiology Microbiota Microorganisms Nature Conservation Nymphs Opportunist infection opportunistic pathogens Pathogenic bacteria Pathogenicity Pathogens Pest control pest management Pests Relative abundance Serratia Serratia marcescens sexual maturity Staphylococcus Water Quality/Water Pollution |
| title | Dynamic Gut Microbiota of Apolygus lucorum Across Different Life Stages Reveals Potential Pathogenic Bacteria for Facilitating the Pest Management |
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