Utility of peripheral blood monocyte subsets, circulating immune complexes and serum cytokines in assessment of SLE activity: an observational, cross-sectional study
Introduction SLE disease measurements by current standards are less than perfect. Monocytes and their subsets are part of innate immunity, and one of our objectives was to look at their role in SLE disease activity. We also looked at the common serum cytokines and the role of circulating immune comp...
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| Veröffentlicht in: | Clinical rheumatology 2024, Vol.43 (1), p.209-217 |
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| creator | Jha, Avanish Joseph, Josna Prabhu, Savit B Chaudhary, Anita Yadav, Bijesh Mathew, John |
| description | Introduction
SLE disease measurements by current standards are less than perfect. Monocytes and their subsets are part of innate immunity, and one of our objectives was to look at their role in SLE disease activity. We also looked at the common serum cytokines and the role of circulating immune complex (CIC) estimation in the assessment of disease activity.
Methods
We conducted a single-centre observational cross-sectional study of SLE patients with active and inactive disease as the comparison arms. Blood samples were collected for (a) peripheral blood monocyte separation and flowcytometric analysis of monocyte subsets based on CD14 and CD16 surface markers, and (b) ELISA for serum cytokines and CIC estimation. Results were analysed in terms of the difference in medians between the active and inactive disease groups using the Mann-Whitney U test (non-normally distributed data).
Results
The absolute monocyte count was lower in the active group than the inactive group (median (IQR) of 329 (228.5) vs. 628 (257)/microliter,
p
= 0.001). The frequency (%) of the intermediate monocyte subset showed a trend towards an increase in active disease (median (IQR) of 15.10% (9.65) vs. 11.85% (8.00),
p
= 0.09). It also had a significant positive correlation to the SLEDAI scores (
r
= 0.33,
p
= 0.046). The mean fluorescence intensity (MFI) of CD163, expressed primarily by intermediate subsets, was increased, and CD11c MFI was reduced in active disease. Serum TNF-a level was elevated in active disease (median (IQR) of 38 (48.5) pg/ml vs. 9 (48.5) pg/ml,
p
= 0.042). CIC ELISA at an optimal cut-off of 10 meq/ml provided an area under the curve (AUC) of 0.85 for detecting active SLE.
Conclusion
Peripheral blood monocytes are depleted in active disease. The intermediate monocyte subset may have a role in disease activity. TNF-alpha correlated modestly with disease activity. CIC estimation by ELISA may be used in addition to or as an alternative to current standards of laboratory tests for the serological assessment of activity. |
| doi_str_mv | 10.1007/s10067-023-06832-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2896806048</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2896806048</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-735d6a2d357fec6a2d0579c53fe6c96e45fa467c4874f83d7a5f57a8bccf60f03</originalsourceid><addsrcrecordid>eNp9kcFuFSEUhonR2NvqC7gwJG5cOAoDA4w709RqchMX2vWEyxwqdQauHKbxPpDvKbdTNXHhBsjhO_9_4CfkGWevOWP6DdZV6Ya1omHKiLZhD8iGSyGbvpf9Q7JhWrNG8N6ckFPEG8ZYa3r-mJwIwyQzWm_Iz6sSplAONHm6hxz2XyHbie6mlEY6p5jcoQDFZYdQ8BV1IbtlsiXEaxrmeYlAXZr3E_wApDaOFCEvM61N6VuItRYitYiAOEMsR5PP2wtqXQm31fRtbaGpSufbKpminapDTogNglsLFMsyHp6QR95OCE_v9zNy9f7iy_mHZvvp8uP5u23jhO5Ko0U3KtuOotMe3PHEOt27TnhQrlcgO2-l0k4aLb0Ro7ad77Q1O-e8Yp6JM_Jy1d3n9H0BLMMc0ME02QhpwaF-nzJMMWkq-uIf9CYtuU5cqZ5zJVtueKXalbp7VgY_7HOYbT4MnA3HEIc1xKGGONyFOByneH4vvexmGP-0_E6tAmIFsF7Fa8h_vf8j-wvQ2Kuc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2911642181</pqid></control><display><type>article</type><title>Utility of peripheral blood monocyte subsets, circulating immune complexes and serum cytokines in assessment of SLE activity: an observational, cross-sectional study</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Jha, Avanish ; Joseph, Josna ; Prabhu, Savit B ; Chaudhary, Anita ; Yadav, Bijesh ; Mathew, John</creator><creatorcontrib>Jha, Avanish ; Joseph, Josna ; Prabhu, Savit B ; Chaudhary, Anita ; Yadav, Bijesh ; Mathew, John</creatorcontrib><description>Introduction
SLE disease measurements by current standards are less than perfect. Monocytes and their subsets are part of innate immunity, and one of our objectives was to look at their role in SLE disease activity. We also looked at the common serum cytokines and the role of circulating immune complex (CIC) estimation in the assessment of disease activity.
Methods
We conducted a single-centre observational cross-sectional study of SLE patients with active and inactive disease as the comparison arms. Blood samples were collected for (a) peripheral blood monocyte separation and flowcytometric analysis of monocyte subsets based on CD14 and CD16 surface markers, and (b) ELISA for serum cytokines and CIC estimation. Results were analysed in terms of the difference in medians between the active and inactive disease groups using the Mann-Whitney U test (non-normally distributed data).
Results
The absolute monocyte count was lower in the active group than the inactive group (median (IQR) of 329 (228.5) vs. 628 (257)/microliter,
p
= 0.001). The frequency (%) of the intermediate monocyte subset showed a trend towards an increase in active disease (median (IQR) of 15.10% (9.65) vs. 11.85% (8.00),
p
= 0.09). It also had a significant positive correlation to the SLEDAI scores (
r
= 0.33,
p
= 0.046). The mean fluorescence intensity (MFI) of CD163, expressed primarily by intermediate subsets, was increased, and CD11c MFI was reduced in active disease. Serum TNF-a level was elevated in active disease (median (IQR) of 38 (48.5) pg/ml vs. 9 (48.5) pg/ml,
p
= 0.042). CIC ELISA at an optimal cut-off of 10 meq/ml provided an area under the curve (AUC) of 0.85 for detecting active SLE.
Conclusion
Peripheral blood monocytes are depleted in active disease. The intermediate monocyte subset may have a role in disease activity. TNF-alpha correlated modestly with disease activity. CIC estimation by ELISA may be used in addition to or as an alternative to current standards of laboratory tests for the serological assessment of activity.</description><identifier>ISSN: 0770-3198</identifier><identifier>EISSN: 1434-9949</identifier><identifier>DOI: 10.1007/s10067-023-06832-0</identifier><identifier>PMID: 38040877</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Antigen-Antibody Complex ; Antigen-antibody complexes ; CD11c antigen ; CD14 antigen ; CD16 antigen ; CD163 antigen ; Cross-Sectional Studies ; Cytokines ; Humans ; Innate immunity ; Lupus Erythematosus, Systemic - diagnosis ; Medicine ; Medicine & Public Health ; Monocytes ; Original Article ; Peripheral blood ; Rheumatology ; Surface markers ; Systemic lupus erythematosus ; Tumor necrosis factor-α</subject><ispartof>Clinical rheumatology, 2024, Vol.43 (1), p.209-217</ispartof><rights>The Author(s), under exclusive licence to International League of Associations for Rheumatology (ILAR) 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to International League of Associations for Rheumatology (ILAR).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-735d6a2d357fec6a2d0579c53fe6c96e45fa467c4874f83d7a5f57a8bccf60f03</citedby><cites>FETCH-LOGICAL-c375t-735d6a2d357fec6a2d0579c53fe6c96e45fa467c4874f83d7a5f57a8bccf60f03</cites><orcidid>0000-0002-3495-0827</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10067-023-06832-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10067-023-06832-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38040877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jha, Avanish</creatorcontrib><creatorcontrib>Joseph, Josna</creatorcontrib><creatorcontrib>Prabhu, Savit B</creatorcontrib><creatorcontrib>Chaudhary, Anita</creatorcontrib><creatorcontrib>Yadav, Bijesh</creatorcontrib><creatorcontrib>Mathew, John</creatorcontrib><title>Utility of peripheral blood monocyte subsets, circulating immune complexes and serum cytokines in assessment of SLE activity: an observational, cross-sectional study</title><title>Clinical rheumatology</title><addtitle>Clin Rheumatol</addtitle><addtitle>Clin Rheumatol</addtitle><description>Introduction
SLE disease measurements by current standards are less than perfect. Monocytes and their subsets are part of innate immunity, and one of our objectives was to look at their role in SLE disease activity. We also looked at the common serum cytokines and the role of circulating immune complex (CIC) estimation in the assessment of disease activity.
Methods
We conducted a single-centre observational cross-sectional study of SLE patients with active and inactive disease as the comparison arms. Blood samples were collected for (a) peripheral blood monocyte separation and flowcytometric analysis of monocyte subsets based on CD14 and CD16 surface markers, and (b) ELISA for serum cytokines and CIC estimation. Results were analysed in terms of the difference in medians between the active and inactive disease groups using the Mann-Whitney U test (non-normally distributed data).
Results
The absolute monocyte count was lower in the active group than the inactive group (median (IQR) of 329 (228.5) vs. 628 (257)/microliter,
p
= 0.001). The frequency (%) of the intermediate monocyte subset showed a trend towards an increase in active disease (median (IQR) of 15.10% (9.65) vs. 11.85% (8.00),
p
= 0.09). It also had a significant positive correlation to the SLEDAI scores (
r
= 0.33,
p
= 0.046). The mean fluorescence intensity (MFI) of CD163, expressed primarily by intermediate subsets, was increased, and CD11c MFI was reduced in active disease. Serum TNF-a level was elevated in active disease (median (IQR) of 38 (48.5) pg/ml vs. 9 (48.5) pg/ml,
p
= 0.042). CIC ELISA at an optimal cut-off of 10 meq/ml provided an area under the curve (AUC) of 0.85 for detecting active SLE.
Conclusion
Peripheral blood monocytes are depleted in active disease. The intermediate monocyte subset may have a role in disease activity. TNF-alpha correlated modestly with disease activity. CIC estimation by ELISA may be used in addition to or as an alternative to current standards of laboratory tests for the serological assessment of activity.</description><subject>Antigen-Antibody Complex</subject><subject>Antigen-antibody complexes</subject><subject>CD11c antigen</subject><subject>CD14 antigen</subject><subject>CD16 antigen</subject><subject>CD163 antigen</subject><subject>Cross-Sectional Studies</subject><subject>Cytokines</subject><subject>Humans</subject><subject>Innate immunity</subject><subject>Lupus Erythematosus, Systemic - diagnosis</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Monocytes</subject><subject>Original Article</subject><subject>Peripheral blood</subject><subject>Rheumatology</subject><subject>Surface markers</subject><subject>Systemic lupus erythematosus</subject><subject>Tumor necrosis factor-α</subject><issn>0770-3198</issn><issn>1434-9949</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFuFSEUhonR2NvqC7gwJG5cOAoDA4w709RqchMX2vWEyxwqdQauHKbxPpDvKbdTNXHhBsjhO_9_4CfkGWevOWP6DdZV6Ya1omHKiLZhD8iGSyGbvpf9Q7JhWrNG8N6ckFPEG8ZYa3r-mJwIwyQzWm_Iz6sSplAONHm6hxz2XyHbie6mlEY6p5jcoQDFZYdQ8BV1IbtlsiXEaxrmeYlAXZr3E_wApDaOFCEvM61N6VuItRYitYiAOEMsR5PP2wtqXQm31fRtbaGpSufbKpminapDTogNglsLFMsyHp6QR95OCE_v9zNy9f7iy_mHZvvp8uP5u23jhO5Ko0U3KtuOotMe3PHEOt27TnhQrlcgO2-l0k4aLb0Ro7ad77Q1O-e8Yp6JM_Jy1d3n9H0BLMMc0ME02QhpwaF-nzJMMWkq-uIf9CYtuU5cqZ5zJVtueKXalbp7VgY_7HOYbT4MnA3HEIc1xKGGONyFOByneH4vvexmGP-0_E6tAmIFsF7Fa8h_vf8j-wvQ2Kuc</recordid><startdate>2024</startdate><enddate>2024</enddate><creator>Jha, Avanish</creator><creator>Joseph, Josna</creator><creator>Prabhu, Savit B</creator><creator>Chaudhary, Anita</creator><creator>Yadav, Bijesh</creator><creator>Mathew, John</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3495-0827</orcidid></search><sort><creationdate>2024</creationdate><title>Utility of peripheral blood monocyte subsets, circulating immune complexes and serum cytokines in assessment of SLE activity: an observational, cross-sectional study</title><author>Jha, Avanish ; Joseph, Josna ; Prabhu, Savit B ; Chaudhary, Anita ; Yadav, Bijesh ; Mathew, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-735d6a2d357fec6a2d0579c53fe6c96e45fa467c4874f83d7a5f57a8bccf60f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antigen-Antibody Complex</topic><topic>Antigen-antibody complexes</topic><topic>CD11c antigen</topic><topic>CD14 antigen</topic><topic>CD16 antigen</topic><topic>CD163 antigen</topic><topic>Cross-Sectional Studies</topic><topic>Cytokines</topic><topic>Humans</topic><topic>Innate immunity</topic><topic>Lupus Erythematosus, Systemic - diagnosis</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Monocytes</topic><topic>Original Article</topic><topic>Peripheral blood</topic><topic>Rheumatology</topic><topic>Surface markers</topic><topic>Systemic lupus erythematosus</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jha, Avanish</creatorcontrib><creatorcontrib>Joseph, Josna</creatorcontrib><creatorcontrib>Prabhu, Savit B</creatorcontrib><creatorcontrib>Chaudhary, Anita</creatorcontrib><creatorcontrib>Yadav, Bijesh</creatorcontrib><creatorcontrib>Mathew, John</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical rheumatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jha, Avanish</au><au>Joseph, Josna</au><au>Prabhu, Savit B</au><au>Chaudhary, Anita</au><au>Yadav, Bijesh</au><au>Mathew, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utility of peripheral blood monocyte subsets, circulating immune complexes and serum cytokines in assessment of SLE activity: an observational, cross-sectional study</atitle><jtitle>Clinical rheumatology</jtitle><stitle>Clin Rheumatol</stitle><addtitle>Clin Rheumatol</addtitle><date>2024</date><risdate>2024</risdate><volume>43</volume><issue>1</issue><spage>209</spage><epage>217</epage><pages>209-217</pages><issn>0770-3198</issn><eissn>1434-9949</eissn><abstract>Introduction
SLE disease measurements by current standards are less than perfect. Monocytes and their subsets are part of innate immunity, and one of our objectives was to look at their role in SLE disease activity. We also looked at the common serum cytokines and the role of circulating immune complex (CIC) estimation in the assessment of disease activity.
Methods
We conducted a single-centre observational cross-sectional study of SLE patients with active and inactive disease as the comparison arms. Blood samples were collected for (a) peripheral blood monocyte separation and flowcytometric analysis of monocyte subsets based on CD14 and CD16 surface markers, and (b) ELISA for serum cytokines and CIC estimation. Results were analysed in terms of the difference in medians between the active and inactive disease groups using the Mann-Whitney U test (non-normally distributed data).
Results
The absolute monocyte count was lower in the active group than the inactive group (median (IQR) of 329 (228.5) vs. 628 (257)/microliter,
p
= 0.001). The frequency (%) of the intermediate monocyte subset showed a trend towards an increase in active disease (median (IQR) of 15.10% (9.65) vs. 11.85% (8.00),
p
= 0.09). It also had a significant positive correlation to the SLEDAI scores (
r
= 0.33,
p
= 0.046). The mean fluorescence intensity (MFI) of CD163, expressed primarily by intermediate subsets, was increased, and CD11c MFI was reduced in active disease. Serum TNF-a level was elevated in active disease (median (IQR) of 38 (48.5) pg/ml vs. 9 (48.5) pg/ml,
p
= 0.042). CIC ELISA at an optimal cut-off of 10 meq/ml provided an area under the curve (AUC) of 0.85 for detecting active SLE.
Conclusion
Peripheral blood monocytes are depleted in active disease. The intermediate monocyte subset may have a role in disease activity. TNF-alpha correlated modestly with disease activity. CIC estimation by ELISA may be used in addition to or as an alternative to current standards of laboratory tests for the serological assessment of activity.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>38040877</pmid><doi>10.1007/s10067-023-06832-0</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-3495-0827</orcidid></addata></record> |
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| ispartof | Clinical rheumatology, 2024, Vol.43 (1), p.209-217 |
| issn | 0770-3198 1434-9949 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Antigen-Antibody Complex Antigen-antibody complexes CD11c antigen CD14 antigen CD16 antigen CD163 antigen Cross-Sectional Studies Cytokines Humans Innate immunity Lupus Erythematosus, Systemic - diagnosis Medicine Medicine & Public Health Monocytes Original Article Peripheral blood Rheumatology Surface markers Systemic lupus erythematosus Tumor necrosis factor-α |
| title | Utility of peripheral blood monocyte subsets, circulating immune complexes and serum cytokines in assessment of SLE activity: an observational, cross-sectional study |
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