Streptozotocin-Induced Diabetic Rats Showed a Differential Glycine Receptor Expression in the Spinal Cord: A GlyR Role in Diabetic Neuropathy

In the spinal cord, attenuation of the inhibitory action of glycine is related to an increase in both inflammatory and diabetic neuropathic pain; however, the glycine receptor involvement in diabetic neuropathy has not been reported. We determined the expression of the glycine receptor subunits (α1–...

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Veröffentlicht in:Neurochemical research 2024-03, Vol.49 (3), p.684-691
Hauptverfasser: Velázquez-Flores, Miguel Ángel, Sánchez-Chávez, Gustavo, Morales-Lázaro, Sara L., Ruiz Esparza-Garrido, Ruth, Canizales-Ontiveros, Alejandro, Salceda, Rocío
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container_issue 3
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container_title Neurochemical research
container_volume 49
creator Velázquez-Flores, Miguel Ángel
Sánchez-Chávez, Gustavo
Morales-Lázaro, Sara L.
Ruiz Esparza-Garrido, Ruth
Canizales-Ontiveros, Alejandro
Salceda, Rocío
description In the spinal cord, attenuation of the inhibitory action of glycine is related to an increase in both inflammatory and diabetic neuropathic pain; however, the glycine receptor involvement in diabetic neuropathy has not been reported. We determined the expression of the glycine receptor subunits (α1–α3 and β) in streptozotocin-induced diabetic Long–Evans rats by qPCR and Western blot. The total mRNA and protein expression (whole spinal cord homogenate) of the α1, α3, and β subunits did not change during diabetes; however, the α2 subunit mRNA, but not the protein, was overexpressed 45 days after diabetes induction. By contrast, the synaptic expression of the α1 and α2 subunits decreased in all the studied stages of diabetes, but that of the α3 subunit increased on day 45 after diabetes induction. Intradermal capsaicin produced higher paw-licking behavior in the streptozotocin-induced diabetic rats than in the control animals. In addition, the nocifensive response was higher at 45 days than at 20 days. During diabetes, the expression of the glycine receptor was altered in the spinal cord, which strongly suggests its involvement in diabetic neuropathy.
doi_str_mv 10.1007/s11064-023-04058-9
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subjects Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Capsaicin
Cell Biology
Diabetes
Diabetes mellitus
Diabetes Mellitus, Experimental - chemically induced
Diabetes Mellitus, Experimental - metabolism
Diabetic Neuropathies - metabolism
Diabetic neuropathy
Gene expression
Glycine
Glycine - metabolism
Inflammation
Licking behavior
mRNA
Neurochemistry
Neurology
Neurosciences
Original Paper
Proteins
Rats
Rats, Long-Evans
Receptors
Receptors, Glycine - genetics
Receptors, Glycine - metabolism
RNA, Messenger - metabolism
Spinal cord
Spinal Cord - metabolism
Streptozocin
title Streptozotocin-Induced Diabetic Rats Showed a Differential Glycine Receptor Expression in the Spinal Cord: A GlyR Role in Diabetic Neuropathy
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