Streptozotocin-Induced Diabetic Rats Showed a Differential Glycine Receptor Expression in the Spinal Cord: A GlyR Role in Diabetic Neuropathy
In the spinal cord, attenuation of the inhibitory action of glycine is related to an increase in both inflammatory and diabetic neuropathic pain; however, the glycine receptor involvement in diabetic neuropathy has not been reported. We determined the expression of the glycine receptor subunits (α1–...
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Veröffentlicht in: | Neurochemical research 2024-03, Vol.49 (3), p.684-691 |
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description | In the spinal cord, attenuation of the inhibitory action of glycine is related to an increase in both inflammatory and diabetic neuropathic pain; however, the glycine receptor involvement in diabetic neuropathy has not been reported. We determined the expression of the glycine receptor subunits (α1–α3 and β) in streptozotocin-induced diabetic Long–Evans rats by qPCR and Western blot. The total mRNA and protein expression (whole spinal cord homogenate) of the α1, α3, and β subunits did not change during diabetes; however, the α2 subunit mRNA, but not the protein, was overexpressed 45 days after diabetes induction. By contrast, the synaptic expression of the α1 and α2 subunits decreased in all the studied stages of diabetes, but that of the α3 subunit increased on day 45 after diabetes induction. Intradermal capsaicin produced higher paw-licking behavior in the streptozotocin-induced diabetic rats than in the control animals. In addition, the nocifensive response was higher at 45 days than at 20 days. During diabetes, the expression of the glycine receptor was altered in the spinal cord, which strongly suggests its involvement in diabetic neuropathy. |
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We determined the expression of the glycine receptor subunits (α1–α3 and β) in streptozotocin-induced diabetic Long–Evans rats by qPCR and Western blot. The total mRNA and protein expression (whole spinal cord homogenate) of the α1, α3, and β subunits did not change during diabetes; however, the α2 subunit mRNA, but not the protein, was overexpressed 45 days after diabetes induction. By contrast, the synaptic expression of the α1 and α2 subunits decreased in all the studied stages of diabetes, but that of the α3 subunit increased on day 45 after diabetes induction. Intradermal capsaicin produced higher paw-licking behavior in the streptozotocin-induced diabetic rats than in the control animals. In addition, the nocifensive response was higher at 45 days than at 20 days. During diabetes, the expression of the glycine receptor was altered in the spinal cord, which strongly suggests its involvement in diabetic neuropathy.</description><identifier>ISSN: 0364-3190</identifier><identifier>ISSN: 1573-6903</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-023-04058-9</identifier><identifier>PMID: 38017313</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Capsaicin ; Cell Biology ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Experimental - chemically induced ; Diabetes Mellitus, Experimental - metabolism ; Diabetic Neuropathies - metabolism ; Diabetic neuropathy ; Gene expression ; Glycine ; Glycine - metabolism ; Inflammation ; Licking behavior ; mRNA ; Neurochemistry ; Neurology ; Neurosciences ; Original Paper ; Proteins ; Rats ; Rats, Long-Evans ; Receptors ; Receptors, Glycine - genetics ; Receptors, Glycine - metabolism ; RNA, Messenger - metabolism ; Spinal cord ; Spinal Cord - metabolism ; Streptozocin</subject><ispartof>Neurochemical research, 2024-03, Vol.49 (3), p.684-691</ispartof><rights>The Author(s) 2023</rights><rights>2023. The Author(s).</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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During diabetes, the expression of the glycine receptor was altered in the spinal cord, which strongly suggests its involvement in diabetic neuropathy.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Capsaicin</subject><subject>Cell Biology</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - chemically induced</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetic Neuropathies - metabolism</subject><subject>Diabetic neuropathy</subject><subject>Gene expression</subject><subject>Glycine</subject><subject>Glycine - metabolism</subject><subject>Inflammation</subject><subject>Licking behavior</subject><subject>mRNA</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Original Paper</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Long-Evans</subject><subject>Receptors</subject><subject>Receptors, Glycine - genetics</subject><subject>Receptors, Glycine - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Spinal cord</subject><subject>Spinal Cord - metabolism</subject><subject>Streptozocin</subject><issn>0364-3190</issn><issn>1573-6903</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kUFv1DAQhS0EokvhD3BAlrhwCYztOIm5VUtbKlUg7cI5cpwx6yobB9tRWf5D_zMOW4rEgdNI8773RqNHyEsGbxlA_S4yBlVZABcFlCCbQj0iKyZrUVQKxGOyApFlwRSckGcx3gBkG2dPyYlogNWCiRW526aAU_I_ffLGjcXV2M8Ge_rB6Q6TM3SjU6Tbnb_NS53X1mLAMTk90MvhkC1IN2iWiEDPf0wBY3R-pG6kaYd0O7kxk2sf-vf0bHFs6MYPuOgPJz7hHPyk0-7wnDyxeoj44n6ekq8X51_WH4vrz5dX67PrwogaUlF2XDZdh6BZZ1FLZaW0TSmrklldVhxAWyWMFSCF5J3uRd2wppK86k1vSiVOyZtj7hT89xljavcuGhwGPaKfY8sbJblUwBf09T_ojZ9DfipTSjRcVkrITPEjZYKPMaBtp-D2OhxaBu1SVnssq81ltb_LapfoV_fRc7fH_sHyp50MiCMQszR-w_D39n9ifwFm0J-i</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Velázquez-Flores, Miguel Ángel</creator><creator>Sánchez-Chávez, Gustavo</creator><creator>Morales-Lázaro, Sara L.</creator><creator>Ruiz Esparza-Garrido, Ruth</creator><creator>Canizales-Ontiveros, Alejandro</creator><creator>Salceda, Rocío</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20240301</creationdate><title>Streptozotocin-Induced Diabetic Rats Showed a Differential Glycine Receptor Expression in the Spinal Cord: A GlyR Role in Diabetic Neuropathy</title><author>Velázquez-Flores, Miguel Ángel ; Sánchez-Chávez, Gustavo ; Morales-Lázaro, Sara L. ; Ruiz Esparza-Garrido, Ruth ; Canizales-Ontiveros, Alejandro ; Salceda, Rocío</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-4b258bbe0a1bfea59f55f845641fa46200af93cf305352bad378186526dcdc493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Capsaicin</topic><topic>Cell Biology</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - chemically induced</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetic Neuropathies - metabolism</topic><topic>Diabetic neuropathy</topic><topic>Gene expression</topic><topic>Glycine</topic><topic>Glycine - metabolism</topic><topic>Inflammation</topic><topic>Licking behavior</topic><topic>mRNA</topic><topic>Neurochemistry</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Original Paper</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Long-Evans</topic><topic>Receptors</topic><topic>Receptors, Glycine - genetics</topic><topic>Receptors, Glycine - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Spinal cord</topic><topic>Spinal Cord - metabolism</topic><topic>Streptozocin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Velázquez-Flores, Miguel Ángel</creatorcontrib><creatorcontrib>Sánchez-Chávez, Gustavo</creatorcontrib><creatorcontrib>Morales-Lázaro, Sara L.</creatorcontrib><creatorcontrib>Ruiz Esparza-Garrido, Ruth</creatorcontrib><creatorcontrib>Canizales-Ontiveros, Alejandro</creatorcontrib><creatorcontrib>Salceda, Rocío</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Velázquez-Flores, Miguel Ángel</au><au>Sánchez-Chávez, Gustavo</au><au>Morales-Lázaro, Sara L.</au><au>Ruiz Esparza-Garrido, Ruth</au><au>Canizales-Ontiveros, Alejandro</au><au>Salceda, Rocío</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Streptozotocin-Induced Diabetic Rats Showed a Differential Glycine Receptor Expression in the Spinal Cord: A GlyR Role in Diabetic Neuropathy</atitle><jtitle>Neurochemical research</jtitle><stitle>Neurochem Res</stitle><addtitle>Neurochem Res</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>49</volume><issue>3</issue><spage>684</spage><epage>691</epage><pages>684-691</pages><issn>0364-3190</issn><issn>1573-6903</issn><eissn>1573-6903</eissn><abstract>In the spinal cord, attenuation of the inhibitory action of glycine is related to an increase in both inflammatory and diabetic neuropathic pain; however, the glycine receptor involvement in diabetic neuropathy has not been reported. We determined the expression of the glycine receptor subunits (α1–α3 and β) in streptozotocin-induced diabetic Long–Evans rats by qPCR and Western blot. The total mRNA and protein expression (whole spinal cord homogenate) of the α1, α3, and β subunits did not change during diabetes; however, the α2 subunit mRNA, but not the protein, was overexpressed 45 days after diabetes induction. By contrast, the synaptic expression of the α1 and α2 subunits decreased in all the studied stages of diabetes, but that of the α3 subunit increased on day 45 after diabetes induction. Intradermal capsaicin produced higher paw-licking behavior in the streptozotocin-induced diabetic rats than in the control animals. In addition, the nocifensive response was higher at 45 days than at 20 days. During diabetes, the expression of the glycine receptor was altered in the spinal cord, which strongly suggests its involvement in diabetic neuropathy.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38017313</pmid><doi>10.1007/s11064-023-04058-9</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biochemistry Biomedical and Life Sciences Biomedicine Capsaicin Cell Biology Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - chemically induced Diabetes Mellitus, Experimental - metabolism Diabetic Neuropathies - metabolism Diabetic neuropathy Gene expression Glycine Glycine - metabolism Inflammation Licking behavior mRNA Neurochemistry Neurology Neurosciences Original Paper Proteins Rats Rats, Long-Evans Receptors Receptors, Glycine - genetics Receptors, Glycine - metabolism RNA, Messenger - metabolism Spinal cord Spinal Cord - metabolism Streptozocin |
title | Streptozotocin-Induced Diabetic Rats Showed a Differential Glycine Receptor Expression in the Spinal Cord: A GlyR Role in Diabetic Neuropathy |
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