In Vitro and in Vivo Antimalarial Activity, Cytotoxicity and Phytochemical HRMS2 Profile of Plants from the Western Pará State, Brazilian Amazonia
Ethnopharmacology and botanical taxonomy are valid criteria used to selecting plants for antimalarial bioprospection purposes. Based on these two criteria, ethanol extracts of 11 plants from Santarém City vicinities, Western Pará State, Brazilian Amazonia, had their in vitro antiplasmodial activity...
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| Veröffentlicht in: | Chemistry & biodiversity 2024-01, Vol.21 (1), p.e202301082-n/a |
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| creator | Viana dos Santos, Maria Beatriz Costa Gontijo, Douglas Alves do Nascimento, Maria Fernanda Paula, Renata Cristina Bezerra Bellei, Jessica Correia Raimundo, Felipe Oliveira Gorza Scopel, Kézia Katiani Oliveira, Alaíde Braga Veras Mourão, Rosa Helena |
| description | Ethnopharmacology and botanical taxonomy are valid criteria used to selecting plants for antimalarial bioprospection purposes. Based on these two criteria, ethanol extracts of 11 plants from Santarém City vicinities, Western Pará State, Brazilian Amazonia, had their in vitro antiplasmodial activity against chloroquine‐resistant Plasmodium falciparum (W2 clone) assessed by the PfLDH method, whereas their cytotoxicity to HepG2‐A16 cells was assessed through MTT assay. Acmella oleracea, Siparuna krukovii and Trema micrantha extracts disclosed the highest rate of parasite growth inhibition (90 %) in screening tests. In vivo antimalarial assays were conducted with these extracts against Plasmodium berghei (NK 65 strain) infected mice. Inhibition rate of parasite multiplication ranged from 41.4 % to 60.9 % at the lowest extract dose (25 mg/kg). HPLC‐ESI‐HRMS2 analyses allowed the putative identification of alkylamides, fatty acids, flavonoid glycosides and alkaloids in ethanol extracts deriving from these three plant species. Results pointed towards A. oleracea flowers ethanol extract as the most promising potential candidate to preclinical studies aiming the development of antimalarial phytomedicine. |
| doi_str_mv | 10.1002/cbdv.202301082 |
| format | Article |
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Based on these two criteria, ethanol extracts of 11 plants from Santarém City vicinities, Western Pará State, Brazilian Amazonia, had their in vitro antiplasmodial activity against chloroquine‐resistant Plasmodium falciparum (W2 clone) assessed by the PfLDH method, whereas their cytotoxicity to HepG2‐A16 cells was assessed through MTT assay. Acmella oleracea, Siparuna krukovii and Trema micrantha extracts disclosed the highest rate of parasite growth inhibition (90 %) in screening tests. In vivo antimalarial assays were conducted with these extracts against Plasmodium berghei (NK 65 strain) infected mice. Inhibition rate of parasite multiplication ranged from 41.4 % to 60.9 % at the lowest extract dose (25 mg/kg). HPLC‐ESI‐HRMS2 analyses allowed the putative identification of alkylamides, fatty acids, flavonoid glycosides and alkaloids in ethanol extracts deriving from these three plant species. Results pointed towards A. oleracea flowers ethanol extract as the most promising potential candidate to preclinical studies aiming the development of antimalarial phytomedicine.</description><identifier>ISSN: 1612-1872</identifier><identifier>EISSN: 1612-1880</identifier><identifier>DOI: 10.1002/cbdv.202301082</identifier><language>eng</language><publisher>Weinheim: Wiley Subscription Services, Inc</publisher><subject>Antimalarial activity ; Antiparasitic agents ; Antiprotozoal agents ; Biocompatibility ; Chloroquine ; Criteria ; Cytotoxicity ; Ethanol ; Ethnopharmacology ; Flavone glycosides ; Flavonoids ; Glycosides ; In vivo methods and tests ; Liquid chromatography ; Malaria ; Parasites ; Plant extracts ; Plant species ; Plants (botany) ; Taxonomy ; Toxicity</subject><ispartof>Chemistry & biodiversity, 2024-01, Vol.21 (1), p.e202301082-n/a</ispartof><rights>2023 Wiley‐VHCA AG, Zurich, Switzerland</rights><rights>2024 Wiley‐VHCA AG, Zurich, Switzerland</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-2497-0020 ; 0000-0002-7759-9694</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbdv.202301082$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbdv.202301082$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Viana dos Santos, Maria Beatriz</creatorcontrib><creatorcontrib>Costa Gontijo, Douglas</creatorcontrib><creatorcontrib>Alves do Nascimento, Maria Fernanda</creatorcontrib><creatorcontrib>Paula, Renata Cristina</creatorcontrib><creatorcontrib>Bezerra Bellei, Jessica Correia</creatorcontrib><creatorcontrib>Raimundo, Felipe Oliveira</creatorcontrib><creatorcontrib>Gorza Scopel, Kézia Katiani</creatorcontrib><creatorcontrib>Oliveira, Alaíde Braga</creatorcontrib><creatorcontrib>Veras Mourão, Rosa Helena</creatorcontrib><title>In Vitro and in Vivo Antimalarial Activity, Cytotoxicity and Phytochemical HRMS2 Profile of Plants from the Western Pará State, Brazilian Amazonia</title><title>Chemistry & biodiversity</title><description>Ethnopharmacology and botanical taxonomy are valid criteria used to selecting plants for antimalarial bioprospection purposes. Based on these two criteria, ethanol extracts of 11 plants from Santarém City vicinities, Western Pará State, Brazilian Amazonia, had their in vitro antiplasmodial activity against chloroquine‐resistant Plasmodium falciparum (W2 clone) assessed by the PfLDH method, whereas their cytotoxicity to HepG2‐A16 cells was assessed through MTT assay. Acmella oleracea, Siparuna krukovii and Trema micrantha extracts disclosed the highest rate of parasite growth inhibition (90 %) in screening tests. In vivo antimalarial assays were conducted with these extracts against Plasmodium berghei (NK 65 strain) infected mice. Inhibition rate of parasite multiplication ranged from 41.4 % to 60.9 % at the lowest extract dose (25 mg/kg). HPLC‐ESI‐HRMS2 analyses allowed the putative identification of alkylamides, fatty acids, flavonoid glycosides and alkaloids in ethanol extracts deriving from these three plant species. Results pointed towards A. oleracea flowers ethanol extract as the most promising potential candidate to preclinical studies aiming the development of antimalarial phytomedicine.</description><subject>Antimalarial activity</subject><subject>Antiparasitic agents</subject><subject>Antiprotozoal agents</subject><subject>Biocompatibility</subject><subject>Chloroquine</subject><subject>Criteria</subject><subject>Cytotoxicity</subject><subject>Ethanol</subject><subject>Ethnopharmacology</subject><subject>Flavone glycosides</subject><subject>Flavonoids</subject><subject>Glycosides</subject><subject>In vivo methods and tests</subject><subject>Liquid chromatography</subject><subject>Malaria</subject><subject>Parasites</subject><subject>Plant extracts</subject><subject>Plant species</subject><subject>Plants (botany)</subject><subject>Taxonomy</subject><subject>Toxicity</subject><issn>1612-1872</issn><issn>1612-1880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpdkctOAzEMRUcIJEphyzoSGxa0OJlXZlnKo0hFVDyXkZsmatDMpGTSQvsbfAHfwo-RAuqClX2tI_vKN4oOKXQpADuV48miy4DFQIGzrahFM8o6lHPY3vQ52432muYl8GHOW9HHdU2ejHeWYD0hZi0WlvRqbyos0RksSU96szB-eUL6S2-9fTcyqB9-NA0TOVWVkQEc3N3cMzJyVptSEavJqMTaN0Q7WxE_VeRZNV65mozQfX2Se49enZAzhytTGqxJr8KVrQ3uRzsay0Yd_NV29Hh58dAfdIa3V9f93rAzo0XGOnEioWCZokpJSHUOOkPIkKIqGEoAlbGUppDlaYqJznPgSUG1ZlxOMj6medyOjn_3zpx9nQdvojKNVGVwrey8EYwXSc5SzpKAHv1DX-zc1cGdYEU4EscJFIEqfqm38IClmLnwRLcUFMQ6ILEOSGwCEv2z86eNir8BnhiHJg</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Viana dos Santos, Maria Beatriz</creator><creator>Costa Gontijo, Douglas</creator><creator>Alves do Nascimento, Maria Fernanda</creator><creator>Paula, Renata Cristina</creator><creator>Bezerra Bellei, Jessica Correia</creator><creator>Raimundo, Felipe Oliveira</creator><creator>Gorza Scopel, Kézia Katiani</creator><creator>Oliveira, Alaíde Braga</creator><creator>Veras Mourão, Rosa Helena</creator><general>Wiley Subscription Services, Inc</general><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2497-0020</orcidid><orcidid>https://orcid.org/0000-0002-7759-9694</orcidid></search><sort><creationdate>202401</creationdate><title>In Vitro and in Vivo Antimalarial Activity, Cytotoxicity and Phytochemical HRMS2 Profile of Plants from the Western Pará State, Brazilian Amazonia</title><author>Viana dos Santos, Maria Beatriz ; Costa Gontijo, Douglas ; Alves do Nascimento, Maria Fernanda ; Paula, Renata Cristina ; Bezerra Bellei, Jessica Correia ; Raimundo, Felipe Oliveira ; Gorza Scopel, Kézia Katiani ; Oliveira, Alaíde Braga ; Veras Mourão, Rosa Helena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1962-34c0926e1eec05f70f6a06a1ae92ac00e6251506755a4f7708491ff28cd68b173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antimalarial activity</topic><topic>Antiparasitic agents</topic><topic>Antiprotozoal agents</topic><topic>Biocompatibility</topic><topic>Chloroquine</topic><topic>Criteria</topic><topic>Cytotoxicity</topic><topic>Ethanol</topic><topic>Ethnopharmacology</topic><topic>Flavone glycosides</topic><topic>Flavonoids</topic><topic>Glycosides</topic><topic>In vivo methods and tests</topic><topic>Liquid chromatography</topic><topic>Malaria</topic><topic>Parasites</topic><topic>Plant extracts</topic><topic>Plant species</topic><topic>Plants (botany)</topic><topic>Taxonomy</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Viana dos Santos, Maria Beatriz</creatorcontrib><creatorcontrib>Costa Gontijo, Douglas</creatorcontrib><creatorcontrib>Alves do Nascimento, Maria Fernanda</creatorcontrib><creatorcontrib>Paula, Renata Cristina</creatorcontrib><creatorcontrib>Bezerra Bellei, Jessica Correia</creatorcontrib><creatorcontrib>Raimundo, Felipe Oliveira</creatorcontrib><creatorcontrib>Gorza Scopel, Kézia Katiani</creatorcontrib><creatorcontrib>Oliveira, Alaíde Braga</creatorcontrib><creatorcontrib>Veras Mourão, Rosa Helena</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry & biodiversity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Viana dos Santos, Maria Beatriz</au><au>Costa Gontijo, Douglas</au><au>Alves do Nascimento, Maria Fernanda</au><au>Paula, Renata Cristina</au><au>Bezerra Bellei, Jessica Correia</au><au>Raimundo, Felipe Oliveira</au><au>Gorza Scopel, Kézia Katiani</au><au>Oliveira, Alaíde Braga</au><au>Veras Mourão, Rosa Helena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro and in Vivo Antimalarial Activity, Cytotoxicity and Phytochemical HRMS2 Profile of Plants from the Western Pará State, Brazilian Amazonia</atitle><jtitle>Chemistry & biodiversity</jtitle><date>2024-01</date><risdate>2024</risdate><volume>21</volume><issue>1</issue><spage>e202301082</spage><epage>n/a</epage><pages>e202301082-n/a</pages><issn>1612-1872</issn><eissn>1612-1880</eissn><abstract>Ethnopharmacology and botanical taxonomy are valid criteria used to selecting plants for antimalarial bioprospection purposes. Based on these two criteria, ethanol extracts of 11 plants from Santarém City vicinities, Western Pará State, Brazilian Amazonia, had their in vitro antiplasmodial activity against chloroquine‐resistant Plasmodium falciparum (W2 clone) assessed by the PfLDH method, whereas their cytotoxicity to HepG2‐A16 cells was assessed through MTT assay. Acmella oleracea, Siparuna krukovii and Trema micrantha extracts disclosed the highest rate of parasite growth inhibition (90 %) in screening tests. In vivo antimalarial assays were conducted with these extracts against Plasmodium berghei (NK 65 strain) infected mice. Inhibition rate of parasite multiplication ranged from 41.4 % to 60.9 % at the lowest extract dose (25 mg/kg). HPLC‐ESI‐HRMS2 analyses allowed the putative identification of alkylamides, fatty acids, flavonoid glycosides and alkaloids in ethanol extracts deriving from these three plant species. Results pointed towards A. oleracea flowers ethanol extract as the most promising potential candidate to preclinical studies aiming the development of antimalarial phytomedicine.</abstract><cop>Weinheim</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/cbdv.202301082</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-2497-0020</orcidid><orcidid>https://orcid.org/0000-0002-7759-9694</orcidid></addata></record> |
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| subjects | Antimalarial activity Antiparasitic agents Antiprotozoal agents Biocompatibility Chloroquine Criteria Cytotoxicity Ethanol Ethnopharmacology Flavone glycosides Flavonoids Glycosides In vivo methods and tests Liquid chromatography Malaria Parasites Plant extracts Plant species Plants (botany) Taxonomy Toxicity |
| title | In Vitro and in Vivo Antimalarial Activity, Cytotoxicity and Phytochemical HRMS2 Profile of Plants from the Western Pará State, Brazilian Amazonia |
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