Hypergravity Increases the Number of Gene Transcripts of Mechanically Gated and Mechanosensitive Ion Channels in Rat Ventricular Cardiomyocytes
Since hypergravity changes the morphological and physiological properties of the heart, it was assumed that the expression of ion channels that respond to cell stretching or compressing, mechanically gated channels (MGC) and mechanosensitive channels (MSC), would be affected. Using RNA transcriptome...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2023-10, Vol.175 (6), p.730-733 |
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| creator | Kamkin, A. G. Mitrokhin, V. M. Kamkina, O. V. Kazansky, V. E. Bilichenko, A. S. Rodina, A. S. Zolotareva, A. D. Zolotarev, V. I. Sutyagin, P. V. Mladenov, M. I. |
| description | Since hypergravity changes the morphological and physiological properties of the heart, it was assumed that the expression of ion channels that respond to cell stretching or compressing, mechanically gated channels (MGC) and mechanosensitive channels (MSC), would be affected. Using RNA transcriptome sequencing, the change in the number of transcripts for MGC and MSC genes was studied in isolated rat ventricular cardiomyocytes under 4
g
hypergravity for 5 days. It was shown for the first time that hypergravity induces changes in the number of transcripts of MGC genes: an increase for TRPC1, TRPC3, TRPM7, TRPP1 (PKD1), TRPP2 (PKD2), TMEM63A, TMEM63B, but a decrease for TRPV2, Piezo1, Piezo2. The number of MSC gene transcripts increases: TREK-1, Kir6.2, Nav1.5, Cav1.2, Cav1.3, Kv7.1, and Kv1.2. This potentially leads to an increase in the expression of MGC and MSC proteins leading to an increase in the net current and, as a result, pathological changes in the heart function. |
| doi_str_mv | 10.1007/s10517-023-05955-3 |
| format | Article |
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g
hypergravity for 5 days. It was shown for the first time that hypergravity induces changes in the number of transcripts of MGC genes: an increase for TRPC1, TRPC3, TRPM7, TRPP1 (PKD1), TRPP2 (PKD2), TMEM63A, TMEM63B, but a decrease for TRPV2, Piezo1, Piezo2. The number of MSC gene transcripts increases: TREK-1, Kir6.2, Nav1.5, Cav1.2, Cav1.3, Kv7.1, and Kv1.2. This potentially leads to an increase in the expression of MGC and MSC proteins leading to an increase in the net current and, as a result, pathological changes in the heart function.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-023-05955-3</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Calcium channels (voltage-gated) ; Cardiomyocytes ; Cell Biology ; Genes ; Heart ; Heart cells ; Hypergravity ; Internal Medicine ; Ion channels ; Laboratory Medicine ; Mechanosensitive channels ; Pathology ; Physiological aspects ; Potassium channels (inwardly-rectifying) ; Potassium channels (voltage-gated) ; RNA ; Sodium channels (voltage-gated) ; Statistics ; Transcriptomes ; Transient receptor potential proteins ; Ventricle</subject><ispartof>Bulletin of experimental biology and medicine, 2023-10, Vol.175 (6), p.730-733</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>COPYRIGHT 2023 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-38f278d3551c6dd16aa65df1ff976f8084d7b57aca50d69e054c4df0c83bba9e3</citedby><cites>FETCH-LOGICAL-c450t-38f278d3551c6dd16aa65df1ff976f8084d7b57aca50d69e054c4df0c83bba9e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-023-05955-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-023-05955-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Kamkin, A. G.</creatorcontrib><creatorcontrib>Mitrokhin, V. M.</creatorcontrib><creatorcontrib>Kamkina, O. V.</creatorcontrib><creatorcontrib>Kazansky, V. E.</creatorcontrib><creatorcontrib>Bilichenko, A. S.</creatorcontrib><creatorcontrib>Rodina, A. S.</creatorcontrib><creatorcontrib>Zolotareva, A. D.</creatorcontrib><creatorcontrib>Zolotarev, V. I.</creatorcontrib><creatorcontrib>Sutyagin, P. V.</creatorcontrib><creatorcontrib>Mladenov, M. I.</creatorcontrib><title>Hypergravity Increases the Number of Gene Transcripts of Mechanically Gated and Mechanosensitive Ion Channels in Rat Ventricular Cardiomyocytes</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><description>Since hypergravity changes the morphological and physiological properties of the heart, it was assumed that the expression of ion channels that respond to cell stretching or compressing, mechanically gated channels (MGC) and mechanosensitive channels (MSC), would be affected. Using RNA transcriptome sequencing, the change in the number of transcripts for MGC and MSC genes was studied in isolated rat ventricular cardiomyocytes under 4
g
hypergravity for 5 days. It was shown for the first time that hypergravity induces changes in the number of transcripts of MGC genes: an increase for TRPC1, TRPC3, TRPM7, TRPP1 (PKD1), TRPP2 (PKD2), TMEM63A, TMEM63B, but a decrease for TRPV2, Piezo1, Piezo2. The number of MSC gene transcripts increases: TREK-1, Kir6.2, Nav1.5, Cav1.2, Cav1.3, Kv7.1, and Kv1.2. This potentially leads to an increase in the expression of MGC and MSC proteins leading to an increase in the net current and, as a result, pathological changes in the heart function.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Calcium channels (voltage-gated)</subject><subject>Cardiomyocytes</subject><subject>Cell Biology</subject><subject>Genes</subject><subject>Heart</subject><subject>Heart cells</subject><subject>Hypergravity</subject><subject>Internal Medicine</subject><subject>Ion channels</subject><subject>Laboratory Medicine</subject><subject>Mechanosensitive channels</subject><subject>Pathology</subject><subject>Physiological aspects</subject><subject>Potassium channels (inwardly-rectifying)</subject><subject>Potassium channels (voltage-gated)</subject><subject>RNA</subject><subject>Sodium channels (voltage-gated)</subject><subject>Statistics</subject><subject>Transcriptomes</subject><subject>Transient receptor potential proteins</subject><subject>Ventricle</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9ktFqFDEUhgdRcK2-gFcBQbyZmsxMZjKXZdHtQq0g1dtwNjnZTZlJ1iRTmKfwlc24BVuRkotD_vP9h3PgL4q3jJ4zSruPkVHOupJWdUl5z3lZPytWjHd1KaqKPS9WNFNlI4R4WbyK8Xb50patil-X8xHDPsCdTTPZOhUQIkaSDkiup3GHgXhDNuiQ3ARwUQV7THHRvqA6gLMKhmEmG0ioCTh9L_uILtpk75BsvSPrLDkcIrGOfINEfqBLwappgEDWELT14-zVnDC-Ll4YGCK-ua9nxffPn27Wl-XV1812fXFVqobTVNbCVJ3QNedMtVqzFqDl2jBj-q41gopGdzvegQJOddsj5Y1qtKFK1Lsd9FifFR9Oc4_B_5wwJjnaqHAYwKGfoqxEzzreCVZn9N0_6K2fgsvbLRTnVc_EA2oPA0rrjE8B1DJUXnS5T0XdVpk6_w-Vn8bRKu_Q2Kw_Mrx_YDggDOkQ_TAl6118DFYnUAUfY0Ajj8GOEGbJqFwyIk8ZkTkj8k9G5LJ0fTLFDLs9hr-nPeH6DS4-vwY</recordid><startdate>20231001</startdate><enddate>20231001</enddate><creator>Kamkin, A. 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I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypergravity Increases the Number of Gene Transcripts of Mechanically Gated and Mechanosensitive Ion Channels in Rat Ventricular Cardiomyocytes</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><date>2023-10-01</date><risdate>2023</risdate><volume>175</volume><issue>6</issue><spage>730</spage><epage>733</epage><pages>730-733</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><abstract>Since hypergravity changes the morphological and physiological properties of the heart, it was assumed that the expression of ion channels that respond to cell stretching or compressing, mechanically gated channels (MGC) and mechanosensitive channels (MSC), would be affected. Using RNA transcriptome sequencing, the change in the number of transcripts for MGC and MSC genes was studied in isolated rat ventricular cardiomyocytes under 4
g
hypergravity for 5 days. It was shown for the first time that hypergravity induces changes in the number of transcripts of MGC genes: an increase for TRPC1, TRPC3, TRPM7, TRPP1 (PKD1), TRPP2 (PKD2), TMEM63A, TMEM63B, but a decrease for TRPV2, Piezo1, Piezo2. The number of MSC gene transcripts increases: TREK-1, Kir6.2, Nav1.5, Cav1.2, Cav1.3, Kv7.1, and Kv1.2. This potentially leads to an increase in the expression of MGC and MSC proteins leading to an increase in the net current and, as a result, pathological changes in the heart function.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s10517-023-05955-3</doi><tpages>4</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Calcium channels (voltage-gated) Cardiomyocytes Cell Biology Genes Heart Heart cells Hypergravity Internal Medicine Ion channels Laboratory Medicine Mechanosensitive channels Pathology Physiological aspects Potassium channels (inwardly-rectifying) Potassium channels (voltage-gated) RNA Sodium channels (voltage-gated) Statistics Transcriptomes Transient receptor potential proteins Ventricle |
| title | Hypergravity Increases the Number of Gene Transcripts of Mechanically Gated and Mechanosensitive Ion Channels in Rat Ventricular Cardiomyocytes |
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