GmWRKY17-mediated transcriptional regulation of GmDREB1D and GmABA2 controls drought tolerance in soybean

Drought affects soybean growth and ultimately led to yield reduction. WRKY transcription factors involve in the regulation of abiotic stress. Few functions of WRKY transcription factors underlying drought tolerance in soybean are clear. Here, we reported a WRKY transcription factor named GmWRKY17 th...

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Veröffentlicht in:	Plant molecular biology 2023-11, Vol.113 (4-5), p.157-170
Hauptverfasser:	Liu, Yi, Cao, Yueping
Format:	Artikel
Sprache:	eng
Schlagworte:	Abscisic acid Analysis Biochemistry bioluminescence assay Biomedical and Life Sciences DNA binding proteins drought Drought Resistance drought tolerance Droughts Gene Expression Regulation, Plant Gene regulation Genes Genetic aspects Genetic transcription Glycine max - genetics hybrids Life Sciences Plant Pathology Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences Plants, Genetically Modified - genetics RNA RNA sequencing sequence analysis Signal transduction Soybean Soybeans Stress, Physiological - genetics transcription (genetics) Transcription factors Transcription Factors - genetics Transcription Factors - metabolism transcriptome Transcriptomes Water loss water stress Yeasts
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creator	Liu, Yi Cao, Yueping
description	Drought affects soybean growth and ultimately led to yield reduction. WRKY transcription factors involve in the regulation of abiotic stress. Few functions of WRKY transcription factors underlying drought tolerance in soybean are clear. Here, we reported a WRKY transcription factor named GmWRKY17 that positively regulates soybean drought tolerance by regulating drought-induced genes and ABA-related genes. Transcriptome sequencing (RNA-Seq) and yeast one hybrid analysis identified downstream genes regulated by GmWRKY17. ChIP-qPCR, EMSA and dual-luciferase reporter assay showed that GmWRKY17 directly bound to the promoters of the GmDREB1D and GmABA2 , and activated their expression under drought stress. Overexpression of GmDREB1D gene enhanced drought tolerance of soybean. Taken together, our study revealed a regulatory mechanism that GmWRKY17 transcription factor may improve soybean drought tolerance by mediating ABA synthesis and DREB signaling pathway. Key message GmWRKY17 improves the drought tolerance of soybean by reducing water loss, promoting ABA synthesis, and activating GmDREB1D and GmABA2 genes.
doi_str_mv	10.1007/s11103-023-01380-2
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WRKY transcription factors involve in the regulation of abiotic stress. Few functions of WRKY transcription factors underlying drought tolerance in soybean are clear. Here, we reported a WRKY transcription factor named GmWRKY17 that positively regulates soybean drought tolerance by regulating drought-induced genes and ABA-related genes. Transcriptome sequencing (RNA-Seq) and yeast one hybrid analysis identified downstream genes regulated by GmWRKY17. ChIP-qPCR, EMSA and dual-luciferase reporter assay showed that GmWRKY17 directly bound to the promoters of the GmDREB1D and GmABA2 , and activated their expression under drought stress. Overexpression of GmDREB1D gene enhanced drought tolerance of soybean. Taken together, our study revealed a regulatory mechanism that GmWRKY17 transcription factor may improve soybean drought tolerance by mediating ABA synthesis and DREB signaling pathway. 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WRKY transcription factors involve in the regulation of abiotic stress. Few functions of WRKY transcription factors underlying drought tolerance in soybean are clear. Here, we reported a WRKY transcription factor named GmWRKY17 that positively regulates soybean drought tolerance by regulating drought-induced genes and ABA-related genes. Transcriptome sequencing (RNA-Seq) and yeast one hybrid analysis identified downstream genes regulated by GmWRKY17. ChIP-qPCR, EMSA and dual-luciferase reporter assay showed that GmWRKY17 directly bound to the promoters of the GmDREB1D and GmABA2 , and activated their expression under drought stress. Overexpression of GmDREB1D gene enhanced drought tolerance of soybean. Taken together, our study revealed a regulatory mechanism that GmWRKY17 transcription factor may improve soybean drought tolerance by mediating ABA synthesis and DREB signaling pathway. 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genetics</topic><topic>hybrids</topic><topic>Life Sciences</topic><topic>Plant Pathology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified - genetics</topic><topic>RNA</topic><topic>RNA sequencing</topic><topic>sequence analysis</topic><topic>Signal transduction</topic><topic>Soybean</topic><topic>Soybeans</topic><topic>Stress, Physiological - genetics</topic><topic>transcription (genetics)</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>transcriptome</topic><topic>Transcriptomes</topic><topic>Water loss</topic><topic>water stress</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yi</creatorcontrib><creatorcontrib>Cao, Yueping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yi</au><au>Cao, Yueping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GmWRKY17-mediated transcriptional regulation of GmDREB1D and GmABA2 controls drought tolerance in soybean</atitle><jtitle>Plant molecular biology</jtitle><stitle>Plant Mol Biol</stitle><addtitle>Plant Mol Biol</addtitle><date>2023-11-01</date><risdate>2023</risdate><volume>113</volume><issue>4-5</issue><spage>157</spage><epage>170</epage><pages>157-170</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Drought affects soybean growth and ultimately led to yield reduction. WRKY transcription factors involve in the regulation of abiotic stress. Few functions of WRKY transcription factors underlying drought tolerance in soybean are clear. Here, we reported a WRKY transcription factor named GmWRKY17 that positively regulates soybean drought tolerance by regulating drought-induced genes and ABA-related genes. Transcriptome sequencing (RNA-Seq) and yeast one hybrid analysis identified downstream genes regulated by GmWRKY17. ChIP-qPCR, EMSA and dual-luciferase reporter assay showed that GmWRKY17 directly bound to the promoters of the GmDREB1D and GmABA2 , and activated their expression under drought stress. Overexpression of GmDREB1D gene enhanced drought tolerance of soybean. Taken together, our study revealed a regulatory mechanism that GmWRKY17 transcription factor may improve soybean drought tolerance by mediating ABA synthesis and DREB signaling pathway. Key message GmWRKY17 improves the drought tolerance of soybean by reducing water loss, promoting ABA synthesis, and activating GmDREB1D and GmABA2 genes.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37973764</pmid><doi>10.1007/s11103-023-01380-2</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-6955-144X</orcidid><orcidid>https://orcid.org/0000-0003-0784-3839</orcidid></addata></record>
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subjects	Abscisic acid Analysis Biochemistry bioluminescence assay Biomedical and Life Sciences DNA binding proteins drought Drought Resistance drought tolerance Droughts Gene Expression Regulation, Plant Gene regulation Genes Genetic aspects Genetic transcription Glycine max - genetics hybrids Life Sciences Plant Pathology Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences Plants, Genetically Modified - genetics RNA RNA sequencing sequence analysis Signal transduction Soybean Soybeans Stress, Physiological - genetics transcription (genetics) Transcription factors Transcription Factors - genetics Transcription Factors - metabolism transcriptome Transcriptomes Water loss water stress Yeasts
title	GmWRKY17-mediated transcriptional regulation of GmDREB1D and GmABA2 controls drought tolerance in soybean
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