Differential expression of circular RNAs in interleukin 6-promoted osteogenic differentiation of human stem cells from apical papilla
Introduction Studies have shown that interleukin 6 (IL-6) can regulate stem cell osteogenic differentiation; however, the exact mechanism is not clear. Circular RNAs (circRNAs) are closed circular non-coding RNAs that are involved in the process of stem cell osteogenic differentiation. Therefore, th...
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| Veröffentlicht in: | Clinical oral investigations 2023-12, Vol.27 (12), p.7765-7776 |
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| creator | Hu, Guang Wu, Laidi Xue, Kaiyang Han, Hao Sun, Yuhui Gan, Kang Zhu, Juanfang Shi, Qi Du, Tianfeng |
| description | Introduction
Studies have shown that interleukin 6 (IL-6) can regulate stem cell osteogenic differentiation; however, the exact mechanism is not clear. Circular RNAs (circRNAs) are closed circular non-coding RNAs that are involved in the process of stem cell osteogenic differentiation. Therefore, the purpose of this present study was to investigate the effect of IL-6 treatment on osteogenic differentiation of human apical tooth papillae stem cells (hSCAPs), and to detect the difference in circRNA expression using gene microarray technology.
Methods
After extraction and identification of hSCAPs, alkaline phosphatase (ALP) activity, alizarin red staining, and calcium ion quantitative assay were used to determine the changes of ALP enzyme, mineralized nodules, and matrix calcium levels before and after IL-6 treatment of hSCAPs gene microarray technology was used to analyze the changes in circRNA expression levels before and after IL-6 induction of mineralization. The four selected circRNAs were validated by qRT-PCR. Moreover, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to predict the potential functions and biological signaling pathways of circRNAs. Finally, these data are integrated and analyzed to construct circRNA-microRNA-mRNA networks.
Results
Alp and Alizarin red staining confirmed that IL-6 promoted the osteogenic differentiation of hSCAPs. The gene microarray results identified 132 differentially expressed circRNAs, of which 117 were upregulated and 15 were downregulated. Bioinformatic analysis predicted that the circRNA-406620/miR-103a-3p/FAT atypical cadherin 4 (FAT4) pathway might be involved in regulating IL-6 to promote osteogenic differentiation of hSCAPs.
Conclusion
Differentially expressed circRNAs might be closely involved in regulating IL-6 to promote osteogenic differentiation of hSCAPs. |
| doi_str_mv | 10.1007/s00784-023-05366-8 |
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| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2889994968</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2889994968</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-c219d9a423005688604356d011317b15bbfd737dbf3b1b4d47c57156ff3d7f873</originalsourceid><addsrcrecordid>eNp9kd9KHTEQxkNRqrV9gV5IwJvebE12skn2UrRaQRSkvQ7Z_NHo7mZNdsE-QN_bHM9pFS-EkBmY33zzwYfQV0q-U0LEYS6fZBWpoSINcF7JD2iXMuAVCEG3XvU76FPOd4RQxgV8RDsgWl5zLnfR35PgvUtunIPusXuckss5xBFHj01IZul1wteXRxmHsbzZpd4t96Xn1ZTiEGdnccyzizduDAbbF7V5o3K7DHrEBRmwcX2fsS97WE_BlINTqX2vP6Ntr_vsvmzqHvp9-uPX8c_q4urs_PjoojJQ87kyNW1tq1kNhDRcSk4YNNwSSoGKjjZd560AYTsPHe2YZcI0gjbce7DCSwF76Ntat3h_WFye1RDyypUeXVyyqqVs25a1XBb04A16F5c0FneqbglhrCEAharXlEkx5-S8mlIYdPqjKFGrkNQ6JFVCUs8hqZX0_kZ66QZn_6_8S6UAsAZyGY03Lr3cfkf2CYtjnfM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2900445033</pqid></control><display><type>article</type><title>Differential expression of circular RNAs in interleukin 6-promoted osteogenic differentiation of human stem cells from apical papilla</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Hu, Guang ; Wu, Laidi ; Xue, Kaiyang ; Han, Hao ; Sun, Yuhui ; Gan, Kang ; Zhu, Juanfang ; Shi, Qi ; Du, Tianfeng</creator><creatorcontrib>Hu, Guang ; Wu, Laidi ; Xue, Kaiyang ; Han, Hao ; Sun, Yuhui ; Gan, Kang ; Zhu, Juanfang ; Shi, Qi ; Du, Tianfeng</creatorcontrib><description>Introduction
Studies have shown that interleukin 6 (IL-6) can regulate stem cell osteogenic differentiation; however, the exact mechanism is not clear. Circular RNAs (circRNAs) are closed circular non-coding RNAs that are involved in the process of stem cell osteogenic differentiation. Therefore, the purpose of this present study was to investigate the effect of IL-6 treatment on osteogenic differentiation of human apical tooth papillae stem cells (hSCAPs), and to detect the difference in circRNA expression using gene microarray technology.
Methods
After extraction and identification of hSCAPs, alkaline phosphatase (ALP) activity, alizarin red staining, and calcium ion quantitative assay were used to determine the changes of ALP enzyme, mineralized nodules, and matrix calcium levels before and after IL-6 treatment of hSCAPs gene microarray technology was used to analyze the changes in circRNA expression levels before and after IL-6 induction of mineralization. The four selected circRNAs were validated by qRT-PCR. Moreover, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to predict the potential functions and biological signaling pathways of circRNAs. Finally, these data are integrated and analyzed to construct circRNA-microRNA-mRNA networks.
Results
Alp and Alizarin red staining confirmed that IL-6 promoted the osteogenic differentiation of hSCAPs. The gene microarray results identified 132 differentially expressed circRNAs, of which 117 were upregulated and 15 were downregulated. Bioinformatic analysis predicted that the circRNA-406620/miR-103a-3p/FAT atypical cadherin 4 (FAT4) pathway might be involved in regulating IL-6 to promote osteogenic differentiation of hSCAPs.
Conclusion
Differentially expressed circRNAs might be closely involved in regulating IL-6 to promote osteogenic differentiation of hSCAPs.</description><identifier>ISSN: 1436-3771</identifier><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-023-05366-8</identifier><identifier>PMID: 37962668</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Alkaline phosphatase ; Biomarkers ; Calcium ; Cell differentiation ; Cell Differentiation - genetics ; Circular RNA ; Cytokines ; Dentistry ; DNA microarrays ; Gene expression ; Gene regulation ; Genomes ; Humans ; Interleukin 6 ; Interleukin-6 - pharmacology ; Medicine ; Mineralization ; miRNA ; mRNA ; Osteogenesis - genetics ; Osteogenic factor ; RNA, Circular - genetics ; RNA, Circular - metabolism ; Stem cells ; Stem Cells - metabolism</subject><ispartof>Clinical oral investigations, 2023-12, Vol.27 (12), p.7765-7776</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-c219d9a423005688604356d011317b15bbfd737dbf3b1b4d47c57156ff3d7f873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-023-05366-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-023-05366-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37962668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Guang</creatorcontrib><creatorcontrib>Wu, Laidi</creatorcontrib><creatorcontrib>Xue, Kaiyang</creatorcontrib><creatorcontrib>Han, Hao</creatorcontrib><creatorcontrib>Sun, Yuhui</creatorcontrib><creatorcontrib>Gan, Kang</creatorcontrib><creatorcontrib>Zhu, Juanfang</creatorcontrib><creatorcontrib>Shi, Qi</creatorcontrib><creatorcontrib>Du, Tianfeng</creatorcontrib><title>Differential expression of circular RNAs in interleukin 6-promoted osteogenic differentiation of human stem cells from apical papilla</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Introduction
Studies have shown that interleukin 6 (IL-6) can regulate stem cell osteogenic differentiation; however, the exact mechanism is not clear. Circular RNAs (circRNAs) are closed circular non-coding RNAs that are involved in the process of stem cell osteogenic differentiation. Therefore, the purpose of this present study was to investigate the effect of IL-6 treatment on osteogenic differentiation of human apical tooth papillae stem cells (hSCAPs), and to detect the difference in circRNA expression using gene microarray technology.
Methods
After extraction and identification of hSCAPs, alkaline phosphatase (ALP) activity, alizarin red staining, and calcium ion quantitative assay were used to determine the changes of ALP enzyme, mineralized nodules, and matrix calcium levels before and after IL-6 treatment of hSCAPs gene microarray technology was used to analyze the changes in circRNA expression levels before and after IL-6 induction of mineralization. The four selected circRNAs were validated by qRT-PCR. Moreover, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to predict the potential functions and biological signaling pathways of circRNAs. Finally, these data are integrated and analyzed to construct circRNA-microRNA-mRNA networks.
Results
Alp and Alizarin red staining confirmed that IL-6 promoted the osteogenic differentiation of hSCAPs. The gene microarray results identified 132 differentially expressed circRNAs, of which 117 were upregulated and 15 were downregulated. Bioinformatic analysis predicted that the circRNA-406620/miR-103a-3p/FAT atypical cadherin 4 (FAT4) pathway might be involved in regulating IL-6 to promote osteogenic differentiation of hSCAPs.
Conclusion
Differentially expressed circRNAs might be closely involved in regulating IL-6 to promote osteogenic differentiation of hSCAPs.</description><subject>Alkaline phosphatase</subject><subject>Biomarkers</subject><subject>Calcium</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - genetics</subject><subject>Circular RNA</subject><subject>Cytokines</subject><subject>Dentistry</subject><subject>DNA microarrays</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Genomes</subject><subject>Humans</subject><subject>Interleukin 6</subject><subject>Interleukin-6 - pharmacology</subject><subject>Medicine</subject><subject>Mineralization</subject><subject>miRNA</subject><subject>mRNA</subject><subject>Osteogenesis - genetics</subject><subject>Osteogenic factor</subject><subject>RNA, Circular - genetics</subject><subject>RNA, Circular - metabolism</subject><subject>Stem cells</subject><subject>Stem Cells - metabolism</subject><issn>1436-3771</issn><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kd9KHTEQxkNRqrV9gV5IwJvebE12skn2UrRaQRSkvQ7Z_NHo7mZNdsE-QN_bHM9pFS-EkBmY33zzwYfQV0q-U0LEYS6fZBWpoSINcF7JD2iXMuAVCEG3XvU76FPOd4RQxgV8RDsgWl5zLnfR35PgvUtunIPusXuckss5xBFHj01IZul1wteXRxmHsbzZpd4t96Xn1ZTiEGdnccyzizduDAbbF7V5o3K7DHrEBRmwcX2fsS97WE_BlINTqX2vP6Ntr_vsvmzqHvp9-uPX8c_q4urs_PjoojJQ87kyNW1tq1kNhDRcSk4YNNwSSoGKjjZd560AYTsPHe2YZcI0gjbce7DCSwF76Ntat3h_WFye1RDyypUeXVyyqqVs25a1XBb04A16F5c0FneqbglhrCEAharXlEkx5-S8mlIYdPqjKFGrkNQ6JFVCUs8hqZX0_kZ66QZn_6_8S6UAsAZyGY03Lr3cfkf2CYtjnfM</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Hu, Guang</creator><creator>Wu, Laidi</creator><creator>Xue, Kaiyang</creator><creator>Han, Hao</creator><creator>Sun, Yuhui</creator><creator>Gan, Kang</creator><creator>Zhu, Juanfang</creator><creator>Shi, Qi</creator><creator>Du, Tianfeng</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20231201</creationdate><title>Differential expression of circular RNAs in interleukin 6-promoted osteogenic differentiation of human stem cells from apical papilla</title><author>Hu, Guang ; Wu, Laidi ; Xue, Kaiyang ; Han, Hao ; Sun, Yuhui ; Gan, Kang ; Zhu, Juanfang ; Shi, Qi ; Du, Tianfeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-c219d9a423005688604356d011317b15bbfd737dbf3b1b4d47c57156ff3d7f873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alkaline phosphatase</topic><topic>Biomarkers</topic><topic>Calcium</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - genetics</topic><topic>Circular RNA</topic><topic>Cytokines</topic><topic>Dentistry</topic><topic>DNA microarrays</topic><topic>Gene expression</topic><topic>Gene regulation</topic><topic>Genomes</topic><topic>Humans</topic><topic>Interleukin 6</topic><topic>Interleukin-6 - pharmacology</topic><topic>Medicine</topic><topic>Mineralization</topic><topic>miRNA</topic><topic>mRNA</topic><topic>Osteogenesis - genetics</topic><topic>Osteogenic factor</topic><topic>RNA, Circular - genetics</topic><topic>RNA, Circular - metabolism</topic><topic>Stem cells</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Guang</creatorcontrib><creatorcontrib>Wu, Laidi</creatorcontrib><creatorcontrib>Xue, Kaiyang</creatorcontrib><creatorcontrib>Han, Hao</creatorcontrib><creatorcontrib>Sun, Yuhui</creatorcontrib><creatorcontrib>Gan, Kang</creatorcontrib><creatorcontrib>Zhu, Juanfang</creatorcontrib><creatorcontrib>Shi, Qi</creatorcontrib><creatorcontrib>Du, Tianfeng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Guang</au><au>Wu, Laidi</au><au>Xue, Kaiyang</au><au>Han, Hao</au><au>Sun, Yuhui</au><au>Gan, Kang</au><au>Zhu, Juanfang</au><au>Shi, Qi</au><au>Du, Tianfeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of circular RNAs in interleukin 6-promoted osteogenic differentiation of human stem cells from apical papilla</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>27</volume><issue>12</issue><spage>7765</spage><epage>7776</epage><pages>7765-7776</pages><issn>1436-3771</issn><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Introduction
Studies have shown that interleukin 6 (IL-6) can regulate stem cell osteogenic differentiation; however, the exact mechanism is not clear. Circular RNAs (circRNAs) are closed circular non-coding RNAs that are involved in the process of stem cell osteogenic differentiation. Therefore, the purpose of this present study was to investigate the effect of IL-6 treatment on osteogenic differentiation of human apical tooth papillae stem cells (hSCAPs), and to detect the difference in circRNA expression using gene microarray technology.
Methods
After extraction and identification of hSCAPs, alkaline phosphatase (ALP) activity, alizarin red staining, and calcium ion quantitative assay were used to determine the changes of ALP enzyme, mineralized nodules, and matrix calcium levels before and after IL-6 treatment of hSCAPs gene microarray technology was used to analyze the changes in circRNA expression levels before and after IL-6 induction of mineralization. The four selected circRNAs were validated by qRT-PCR. Moreover, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to predict the potential functions and biological signaling pathways of circRNAs. Finally, these data are integrated and analyzed to construct circRNA-microRNA-mRNA networks.
Results
Alp and Alizarin red staining confirmed that IL-6 promoted the osteogenic differentiation of hSCAPs. The gene microarray results identified 132 differentially expressed circRNAs, of which 117 were upregulated and 15 were downregulated. Bioinformatic analysis predicted that the circRNA-406620/miR-103a-3p/FAT atypical cadherin 4 (FAT4) pathway might be involved in regulating IL-6 to promote osteogenic differentiation of hSCAPs.
Conclusion
Differentially expressed circRNAs might be closely involved in regulating IL-6 to promote osteogenic differentiation of hSCAPs.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37962668</pmid><doi>10.1007/s00784-023-05366-8</doi><tpages>12</tpages></addata></record> |
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| subjects | Alkaline phosphatase Biomarkers Calcium Cell differentiation Cell Differentiation - genetics Circular RNA Cytokines Dentistry DNA microarrays Gene expression Gene regulation Genomes Humans Interleukin 6 Interleukin-6 - pharmacology Medicine Mineralization miRNA mRNA Osteogenesis - genetics Osteogenic factor RNA, Circular - genetics RNA, Circular - metabolism Stem cells Stem Cells - metabolism |
| title | Differential expression of circular RNAs in interleukin 6-promoted osteogenic differentiation of human stem cells from apical papilla |
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