β-Carotene laden antibacterial and antioxidant gelatin/polyglyceryl stearate nano-hydrogel system for burn wound healing application
Worldwide, burn wounds are severe health issues prone to bacterial infections and challenging to treat with traditional wound dressings. Therefore, a highly desirable biological macromolecules-based wound dressing with good antioxidant, antibacterial, biocompatible, and a large surface area is requi...
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| Veröffentlicht in: | International journal of biological macromolecules 2024-01, Vol.255, p.128019-128019, Article 128019 |
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| container_title | International journal of biological macromolecules |
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| creator | Darban, Zenab Singh, Hemant Singh, Udisha Bhatia, Dhiraj Gaur, Rama Kuddushi, Muzammil Dhanka, Mukesh Shahabuddin, Syed |
| description | Worldwide, burn wounds are severe health issues prone to bacterial infections and challenging to treat with traditional wound dressings. Therefore, a highly desirable biological macromolecules-based wound dressing with good antioxidant, antibacterial, biocompatible, and a large surface area is required. Herein, aim to develop a biological macromolecules-based physically cross-linked gelatin/polyglyceryl stearate/graphene oxide (GPGO) hydrogel to treat burn wounds. Four sets of hydrogels were prepared by varying GO concentrations. FT-IR, FE-SEM, viscosity analysis, mechanical and thermal stability confirmed the successful preparation of hydrogels with desired properties. Further, β-carotene (0.5 mg/mL) was encapsulated in hydrogels to enhance the antioxidant activity, and a cumulative release as well as kinetics at pH 6.4 and 7.4 was performed. With an increase in GO concentration, hydrogels showed sustained release of β-carotene. Among all, GPGO-3 β hydrogel showed the highest antioxidant potency (57.75 %), hemocompatible ( |
| doi_str_mv | 10.1016/j.ijbiomac.2023.128019 |
| format | Article |
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Therefore, a highly desirable biological macromolecules-based wound dressing with good antioxidant, antibacterial, biocompatible, and a large surface area is required. Herein, aim to develop a biological macromolecules-based physically cross-linked gelatin/polyglyceryl stearate/graphene oxide (GPGO) hydrogel to treat burn wounds. Four sets of hydrogels were prepared by varying GO concentrations. FT-IR, FE-SEM, viscosity analysis, mechanical and thermal stability confirmed the successful preparation of hydrogels with desired properties. Further, β-carotene (0.5 mg/mL) was encapsulated in hydrogels to enhance the antioxidant activity, and a cumulative release as well as kinetics at pH 6.4 and 7.4 was performed. With an increase in GO concentration, hydrogels showed sustained release of β-carotene. Among all, GPGO-3 β hydrogel showed the highest antioxidant potency (57.75 %), hemocompatible (<5 %), cytocompatible (viable with NIH 3T3 cells), cell migration, proliferation, and in vitro wound healing. Also, GPGO-3 β hydrogel showed efficient antibacterial activity (%inhibition of 85.5 % and 80.2 % and zone of 11 mm and 9.8 mm against S. aureus and E. coli). These results demonstrated the ability of GPGO-3 β hydrogel as a promising candidate for burn wound healing applications.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2023.128019</identifier><identifier>PMID: 37952802</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; antibacterial properties ; antioxidant activity ; antioxidants ; Antioxidants - pharmacology ; beta Carotene ; Burns - drug therapy ; cell movement ; crosslinking ; Escherichia coli ; gelatin ; Gelatin - chemistry ; graphene oxide ; Humans ; hydrogels ; Hydrogels - chemistry ; Hydrogels - pharmacology ; Mice ; Spectroscopy, Fourier Transform Infrared ; Staphylococcus aureus ; Stearates ; stearic acid ; surface area ; thermal stability ; viscosity ; Wound Healing</subject><ispartof>International journal of biological macromolecules, 2024-01, Vol.255, p.128019-128019, Article 128019</ispartof><rights>Copyright © 2023. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-2c27e23501e40a2232ca27cb252f9349ca64c2a89aacf36af2dfddde485f49393</citedby><cites>FETCH-LOGICAL-c344t-2c27e23501e40a2232ca27cb252f9349ca64c2a89aacf36af2dfddde485f49393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37952802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Darban, Zenab</creatorcontrib><creatorcontrib>Singh, Hemant</creatorcontrib><creatorcontrib>Singh, Udisha</creatorcontrib><creatorcontrib>Bhatia, Dhiraj</creatorcontrib><creatorcontrib>Gaur, Rama</creatorcontrib><creatorcontrib>Kuddushi, Muzammil</creatorcontrib><creatorcontrib>Dhanka, Mukesh</creatorcontrib><creatorcontrib>Shahabuddin, Syed</creatorcontrib><title>β-Carotene laden antibacterial and antioxidant gelatin/polyglyceryl stearate nano-hydrogel system for burn wound healing application</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Worldwide, burn wounds are severe health issues prone to bacterial infections and challenging to treat with traditional wound dressings. Therefore, a highly desirable biological macromolecules-based wound dressing with good antioxidant, antibacterial, biocompatible, and a large surface area is required. Herein, aim to develop a biological macromolecules-based physically cross-linked gelatin/polyglyceryl stearate/graphene oxide (GPGO) hydrogel to treat burn wounds. Four sets of hydrogels were prepared by varying GO concentrations. FT-IR, FE-SEM, viscosity analysis, mechanical and thermal stability confirmed the successful preparation of hydrogels with desired properties. Further, β-carotene (0.5 mg/mL) was encapsulated in hydrogels to enhance the antioxidant activity, and a cumulative release as well as kinetics at pH 6.4 and 7.4 was performed. With an increase in GO concentration, hydrogels showed sustained release of β-carotene. Among all, GPGO-3 β hydrogel showed the highest antioxidant potency (57.75 %), hemocompatible (<5 %), cytocompatible (viable with NIH 3T3 cells), cell migration, proliferation, and in vitro wound healing. Also, GPGO-3 β hydrogel showed efficient antibacterial activity (%inhibition of 85.5 % and 80.2 % and zone of 11 mm and 9.8 mm against S. aureus and E. coli). These results demonstrated the ability of GPGO-3 β hydrogel as a promising candidate for burn wound healing applications.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibacterial properties</subject><subject>antioxidant activity</subject><subject>antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>beta Carotene</subject><subject>Burns - drug therapy</subject><subject>cell movement</subject><subject>crosslinking</subject><subject>Escherichia coli</subject><subject>gelatin</subject><subject>Gelatin - chemistry</subject><subject>graphene oxide</subject><subject>Humans</subject><subject>hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogels - pharmacology</subject><subject>Mice</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Staphylococcus aureus</subject><subject>Stearates</subject><subject>stearic acid</subject><subject>surface area</subject><subject>thermal stability</subject><subject>viscosity</subject><subject>Wound Healing</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EotPCK1RessnUf0nsJRpBQarEBtbWje1MPXLsYCeCPAAv1AfhmXCZli2ro3vvOecuPoSuKdlTQrub096fBp8mMHtGGN9TJglVL9COyl41hBD-Eu0IFbSRlJMLdFnKqW67lsrX6IL3qq0BtkO_fj80B8hpcdHhANZFDHHxA5jFZQ-hTvbvJv30tio-ugCLjzdzCtsxbMblLeCyOMiwOBwhpuZ-szlVHy5bPUx4TBkPa474R1pr272D4OMRwzwHb2pZim_QqxFCcW-f9Ap9-_jh6-FTc_fl9vPh_V1juBBLwwzrHeMtoU4QYIwzA6w3A2vZqLhQBjphGEgFYEbewcjsaK11QrajUFzxK_Tu3Dvn9H11ZdGTL8aFANGltWhOBBGS9G3_XyuTUrVSScGrtTtbTU6lZDfqOfsJ8qYp0Y-09Ek_09KPtPSZVg1eP_1Yh8nZf7FnPPwP3IWXcQ</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Darban, Zenab</creator><creator>Singh, Hemant</creator><creator>Singh, Udisha</creator><creator>Bhatia, Dhiraj</creator><creator>Gaur, Rama</creator><creator>Kuddushi, Muzammil</creator><creator>Dhanka, Mukesh</creator><creator>Shahabuddin, Syed</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240101</creationdate><title>β-Carotene laden antibacterial and antioxidant gelatin/polyglyceryl stearate nano-hydrogel system for burn wound healing application</title><author>Darban, Zenab ; Singh, Hemant ; Singh, Udisha ; Bhatia, Dhiraj ; Gaur, Rama ; Kuddushi, Muzammil ; Dhanka, Mukesh ; Shahabuddin, Syed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-2c27e23501e40a2232ca27cb252f9349ca64c2a89aacf36af2dfddde485f49393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibacterial properties</topic><topic>antioxidant activity</topic><topic>antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>beta Carotene</topic><topic>Burns - drug therapy</topic><topic>cell movement</topic><topic>crosslinking</topic><topic>Escherichia coli</topic><topic>gelatin</topic><topic>Gelatin - chemistry</topic><topic>graphene oxide</topic><topic>Humans</topic><topic>hydrogels</topic><topic>Hydrogels - chemistry</topic><topic>Hydrogels - pharmacology</topic><topic>Mice</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Staphylococcus aureus</topic><topic>Stearates</topic><topic>stearic acid</topic><topic>surface area</topic><topic>thermal stability</topic><topic>viscosity</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Darban, Zenab</creatorcontrib><creatorcontrib>Singh, Hemant</creatorcontrib><creatorcontrib>Singh, Udisha</creatorcontrib><creatorcontrib>Bhatia, Dhiraj</creatorcontrib><creatorcontrib>Gaur, Rama</creatorcontrib><creatorcontrib>Kuddushi, Muzammil</creatorcontrib><creatorcontrib>Dhanka, Mukesh</creatorcontrib><creatorcontrib>Shahabuddin, Syed</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Darban, Zenab</au><au>Singh, Hemant</au><au>Singh, Udisha</au><au>Bhatia, Dhiraj</au><au>Gaur, Rama</au><au>Kuddushi, Muzammil</au><au>Dhanka, Mukesh</au><au>Shahabuddin, Syed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-Carotene laden antibacterial and antioxidant gelatin/polyglyceryl stearate nano-hydrogel system for burn wound healing application</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>255</volume><spage>128019</spage><epage>128019</epage><pages>128019-128019</pages><artnum>128019</artnum><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Worldwide, burn wounds are severe health issues prone to bacterial infections and challenging to treat with traditional wound dressings. Therefore, a highly desirable biological macromolecules-based wound dressing with good antioxidant, antibacterial, biocompatible, and a large surface area is required. Herein, aim to develop a biological macromolecules-based physically cross-linked gelatin/polyglyceryl stearate/graphene oxide (GPGO) hydrogel to treat burn wounds. Four sets of hydrogels were prepared by varying GO concentrations. FT-IR, FE-SEM, viscosity analysis, mechanical and thermal stability confirmed the successful preparation of hydrogels with desired properties. Further, β-carotene (0.5 mg/mL) was encapsulated in hydrogels to enhance the antioxidant activity, and a cumulative release as well as kinetics at pH 6.4 and 7.4 was performed. With an increase in GO concentration, hydrogels showed sustained release of β-carotene. Among all, GPGO-3 β hydrogel showed the highest antioxidant potency (57.75 %), hemocompatible (<5 %), cytocompatible (viable with NIH 3T3 cells), cell migration, proliferation, and in vitro wound healing. Also, GPGO-3 β hydrogel showed efficient antibacterial activity (%inhibition of 85.5 % and 80.2 % and zone of 11 mm and 9.8 mm against S. aureus and E. coli). These results demonstrated the ability of GPGO-3 β hydrogel as a promising candidate for burn wound healing applications.</abstract><cop>Netherlands</cop><pmid>37952802</pmid><doi>10.1016/j.ijbiomac.2023.128019</doi><tpages>1</tpages></addata></record> |
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| ispartof | International journal of biological macromolecules, 2024-01, Vol.255, p.128019-128019, Article 128019 |
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| subjects | Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology antibacterial properties antioxidant activity antioxidants Antioxidants - pharmacology beta Carotene Burns - drug therapy cell movement crosslinking Escherichia coli gelatin Gelatin - chemistry graphene oxide Humans hydrogels Hydrogels - chemistry Hydrogels - pharmacology Mice Spectroscopy, Fourier Transform Infrared Staphylococcus aureus Stearates stearic acid surface area thermal stability viscosity Wound Healing |
| title | β-Carotene laden antibacterial and antioxidant gelatin/polyglyceryl stearate nano-hydrogel system for burn wound healing application |
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