The effect of bovine trypsin on dental biofilm dispersion : an in vitro study
[Abstract] To investigate the degradation effect of bovine trypsin on multispecies biofilm of caries-related bacteria and provide an experimental foundation for the prevention of dental caries. Standard strains of S. mutans, S. sanguis, S. gordonii, and L. acidophilus were co-cultured to form 24 h,...
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| Veröffentlicht in: | Odontology 2024-04, Vol.112 (2), p.501-511 |
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| creator | Huang, Yinxue Han, Qunchao Zhou, Jing Meng, Xinhui Huo, Lijun Lei, Yayan |
| description | [Abstract] To investigate the degradation effect of bovine trypsin on multispecies biofilm of caries-related bacteria and provide an experimental foundation for the prevention of dental caries. Standard strains of S. mutans, S. sanguis, S. gordonii, and L. acidophilus were co-cultured to form 24 h, 48 h, and 72 h biofilms. The experimental groups were treated with bovine trypsin for 30 s, 1 min, and 3 min. Morphological observation and quantitative analysis of extracellular polymeric substances (EPS), live bacteria, and dead bacteria were conducted using the confocal laser scanning microscope (CLSM). The morphological changes of EPS and bacteria were also observed using a scanning electron microscope (SEM). When biofilm was treated for 1 min, the minimal inhibitory concentration (MIC) of bovine trypsin to reduce EPS was 0.5 mg/mL in 24 h and 48 h biofilms, and the MIC of bovine trypsin was 2.5 mg/mL in 72 h biofilms (P |
| doi_str_mv | 10.1007/s10266-023-00869-y |
| format | Article |
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Standard strains of S. mutans, S. sanguis, S. gordonii, and L. acidophilus were co-cultured to form 24 h, 48 h, and 72 h biofilms. The experimental groups were treated with bovine trypsin for 30 s, 1 min, and 3 min. Morphological observation and quantitative analysis of extracellular polymeric substances (EPS), live bacteria, and dead bacteria were conducted using the confocal laser scanning microscope (CLSM). The morphological changes of EPS and bacteria were also observed using a scanning electron microscope (SEM). When biofilm was treated for 1 min, the minimal inhibitory concentration (MIC) of bovine trypsin to reduce EPS was 0.5 mg/mL in 24 h and 48 h biofilms, and the MIC of bovine trypsin was 2.5 mg/mL in 72 h biofilms (P<0.05). When biofilm was treated for 3 min, the MIC of bovine trypsin to reduce EPS was 0.25 mg/mL in 24 h and 48 h biofilms, the MIC of bovine trypsin was 1 mg/mL in 72 h biofilm (P<0.05). The ratio of live-to-dead bacteria in the treatment group was significantly lower than blank group in 24 h, 48 h, and 72 h multispecies biofilms (P<0.05). Bovine trypsin can destroy multispecies biofilm structure, disperse biofilm and bacteria flora, and reduce the EPS and bacterial biomass in vitro, which are positively correlated with the application time and concentration.</description><identifier>ISSN: 1618-1247</identifier><identifier>EISSN: 1618-1255</identifier><identifier>DOI: 10.1007/s10266-023-00869-y</identifier><identifier>PMID: 37955766</identifier><language>eng</language><publisher>Singapore: The Society of the Nippon Dental University</publisher><subject>Bacteria ; Biofilms ; Dental caries ; Dentistry ; Extracellular polymers ; Medicine ; Minimum inhibitory concentration ; Morphology ; Oral and Maxillofacial Surgery ; Original Article ; Scanning electron microscopy ; Trypsin</subject><ispartof>Odontology, 2024-04, Vol.112 (2), p.501-511</ispartof><rights>The Author(s), under exclusive licence to The Society of The Nippon Dental University 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to The Society of The Nippon Dental University.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c390t-7ae6f24605549980a5c75d45436329ab48ecc48a5e1dce57d6e485a99a43098c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10266-023-00869-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10266-023-00869-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37955766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yinxue</creatorcontrib><creatorcontrib>Han, Qunchao</creatorcontrib><creatorcontrib>Zhou, Jing</creatorcontrib><creatorcontrib>Meng, Xinhui</creatorcontrib><creatorcontrib>Huo, Lijun</creatorcontrib><creatorcontrib>Lei, Yayan</creatorcontrib><creatorcontrib>Department of Operative Dentistry</creatorcontrib><creatorcontrib>Kunming Medical University and Hospital of Stomatology</creatorcontrib><creatorcontrib>Preventive Dentistry and Endodontics</creatorcontrib><creatorcontrib>Yunnan Key Laboratory of Stomatology</creatorcontrib><title>The effect of bovine trypsin on dental biofilm dispersion : an in vitro study</title><title>Odontology</title><addtitle>Odontology</addtitle><addtitle>Odontology</addtitle><description>[Abstract] To investigate the degradation effect of bovine trypsin on multispecies biofilm of caries-related bacteria and provide an experimental foundation for the prevention of dental caries. Standard strains of S. mutans, S. sanguis, S. gordonii, and L. acidophilus were co-cultured to form 24 h, 48 h, and 72 h biofilms. The experimental groups were treated with bovine trypsin for 30 s, 1 min, and 3 min. Morphological observation and quantitative analysis of extracellular polymeric substances (EPS), live bacteria, and dead bacteria were conducted using the confocal laser scanning microscope (CLSM). The morphological changes of EPS and bacteria were also observed using a scanning electron microscope (SEM). When biofilm was treated for 1 min, the minimal inhibitory concentration (MIC) of bovine trypsin to reduce EPS was 0.5 mg/mL in 24 h and 48 h biofilms, and the MIC of bovine trypsin was 2.5 mg/mL in 72 h biofilms (P<0.05). When biofilm was treated for 3 min, the MIC of bovine trypsin to reduce EPS was 0.25 mg/mL in 24 h and 48 h biofilms, the MIC of bovine trypsin was 1 mg/mL in 72 h biofilm (P<0.05). The ratio of live-to-dead bacteria in the treatment group was significantly lower than blank group in 24 h, 48 h, and 72 h multispecies biofilms (P<0.05). Bovine trypsin can destroy multispecies biofilm structure, disperse biofilm and bacteria flora, and reduce the EPS and bacterial biomass in vitro, which are positively correlated with the application time and concentration.</description><subject>Bacteria</subject><subject>Biofilms</subject><subject>Dental caries</subject><subject>Dentistry</subject><subject>Extracellular polymers</subject><subject>Medicine</subject><subject>Minimum inhibitory concentration</subject><subject>Morphology</subject><subject>Oral and Maxillofacial Surgery</subject><subject>Original Article</subject><subject>Scanning electron microscopy</subject><subject>Trypsin</subject><issn>1618-1247</issn><issn>1618-1255</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kU1rFjEUhYMottT-ARcScONm9N58TeJOSq1CxU1dh0wmUyPzJq_JTGH-vdGpLbhwkxO4zzk3nBDyEuEtAvTvKgJTqgPGOwCtTLc9IaeoUHfIpHz6cBf9CTmvNQ4gGCL0KJ-TE94bKXulTsmXm--BhmkKfqF5okO-iynQpWzHGhPNiY4hLW6mQ8xTnA90jPUYSo1t8p66RBt0F5eSaV3WcXtBnk1uruH8Xs_It4-XNxefuuuvV58vPlx3nhtYut4FNTGhQEphjAYnfS9HIQVXnBk3CB28F9rJgKMPsh9VEFo6Y5zgYLTnZ-TNnnss-eca6mIPsfowzy6FvFbLtDZSa0TZ0Nf_oD_yWlJ7nWVGCq2U4KZRbKd8ybWWMNljiQdXNotgf_dt975t69v-6dtuzfTqPnodDmF8sPxttwF8B2obpdtQHnf_N_Zqd7XM6N2c09z-5NHqbzGPOS2WARMWEJs20RYkYDsQhQBoifwXaXyf8g</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Huang, Yinxue</creator><creator>Han, Qunchao</creator><creator>Zhou, Jing</creator><creator>Meng, Xinhui</creator><creator>Huo, Lijun</creator><creator>Lei, Yayan</creator><general>The Society of the Nippon Dental University</general><general>Springer Nature Singapore</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20240401</creationdate><title>The effect of bovine trypsin on dental biofilm dispersion : an in vitro study</title><author>Huang, Yinxue ; Han, Qunchao ; Zhou, Jing ; Meng, Xinhui ; Huo, Lijun ; Lei, Yayan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-7ae6f24605549980a5c75d45436329ab48ecc48a5e1dce57d6e485a99a43098c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Bacteria</topic><topic>Biofilms</topic><topic>Dental caries</topic><topic>Dentistry</topic><topic>Extracellular polymers</topic><topic>Medicine</topic><topic>Minimum inhibitory concentration</topic><topic>Morphology</topic><topic>Oral and Maxillofacial Surgery</topic><topic>Original Article</topic><topic>Scanning electron microscopy</topic><topic>Trypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yinxue</creatorcontrib><creatorcontrib>Han, Qunchao</creatorcontrib><creatorcontrib>Zhou, Jing</creatorcontrib><creatorcontrib>Meng, Xinhui</creatorcontrib><creatorcontrib>Huo, Lijun</creatorcontrib><creatorcontrib>Lei, Yayan</creatorcontrib><creatorcontrib>Department of Operative Dentistry</creatorcontrib><creatorcontrib>Kunming Medical University and Hospital of Stomatology</creatorcontrib><creatorcontrib>Preventive Dentistry and Endodontics</creatorcontrib><creatorcontrib>Yunnan Key Laboratory of Stomatology</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Odontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yinxue</au><au>Han, Qunchao</au><au>Zhou, Jing</au><au>Meng, Xinhui</au><au>Huo, Lijun</au><au>Lei, Yayan</au><aucorp>Department of Operative Dentistry</aucorp><aucorp>Kunming Medical University and Hospital of Stomatology</aucorp><aucorp>Preventive Dentistry and Endodontics</aucorp><aucorp>Yunnan Key Laboratory of Stomatology</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of bovine trypsin on dental biofilm dispersion : an in vitro study</atitle><jtitle>Odontology</jtitle><stitle>Odontology</stitle><addtitle>Odontology</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>112</volume><issue>2</issue><spage>501</spage><epage>511</epage><pages>501-511</pages><issn>1618-1247</issn><eissn>1618-1255</eissn><abstract>[Abstract] To investigate the degradation effect of bovine trypsin on multispecies biofilm of caries-related bacteria and provide an experimental foundation for the prevention of dental caries. Standard strains of S. mutans, S. sanguis, S. gordonii, and L. acidophilus were co-cultured to form 24 h, 48 h, and 72 h biofilms. The experimental groups were treated with bovine trypsin for 30 s, 1 min, and 3 min. Morphological observation and quantitative analysis of extracellular polymeric substances (EPS), live bacteria, and dead bacteria were conducted using the confocal laser scanning microscope (CLSM). The morphological changes of EPS and bacteria were also observed using a scanning electron microscope (SEM). When biofilm was treated for 1 min, the minimal inhibitory concentration (MIC) of bovine trypsin to reduce EPS was 0.5 mg/mL in 24 h and 48 h biofilms, and the MIC of bovine trypsin was 2.5 mg/mL in 72 h biofilms (P<0.05). When biofilm was treated for 3 min, the MIC of bovine trypsin to reduce EPS was 0.25 mg/mL in 24 h and 48 h biofilms, the MIC of bovine trypsin was 1 mg/mL in 72 h biofilm (P<0.05). The ratio of live-to-dead bacteria in the treatment group was significantly lower than blank group in 24 h, 48 h, and 72 h multispecies biofilms (P<0.05). Bovine trypsin can destroy multispecies biofilm structure, disperse biofilm and bacteria flora, and reduce the EPS and bacterial biomass in vitro, which are positively correlated with the application time and concentration.</abstract><cop>Singapore</cop><pub>The Society of the Nippon Dental University</pub><pmid>37955766</pmid><doi>10.1007/s10266-023-00869-y</doi><tpages>11</tpages></addata></record> |
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| subjects | Bacteria Biofilms Dental caries Dentistry Extracellular polymers Medicine Minimum inhibitory concentration Morphology Oral and Maxillofacial Surgery Original Article Scanning electron microscopy Trypsin |
| title | The effect of bovine trypsin on dental biofilm dispersion : an in vitro study |
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