Immobilization of Horseradish Peroxidase on Ca Alginate-Starch Hybrid Support: Biocatalytic Properties and Application in Biodegradation of Phenol Red Dye

In this study, horseradish peroxidase was extracted, purified, and immobilized on a calcium alginate-starch hybrid support by covalent bonding and entrapment. The immobilized HRP was used for the biodegradation of phenol red dye. A 3.74-fold purification was observed after precipitation with ammoniu...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2024-08, Vol.196 (8), p.4759-4792
Hauptverfasser:	Weber, Ani Caroline, da Silva, Bruno Eduardo, Cordeiro, Sabrina Grando, Henn, Guilherme Schwingel, Costa, Bruna, dos Santos, Jéssica Samara Herek, Corbellini, Valeriano Antonio, Ethur, Eduardo Miranda, Hoehne, Lucélia
Format:	Artikel
Sprache:	eng
Schlagworte:	Alginates - chemistry Alginic acid Ammonium Ammonium sulfate Biocatalysis Biochemistry Biodegradation Biodegradation, Environmental Biotechnology Calcium alginate Chemistry Chemistry and Materials Science Coloring Agents - chemistry Coloring Agents - metabolism Dialysis Dyes Entrapment Enzymatic activity Enzyme Stability Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Free form Glucuronic Acid - chemistry Hexuronic Acids - chemistry Horseradish peroxidase Horseradish Peroxidase - chemistry Horseradish Peroxidase - metabolism Hydrogen-Ion Concentration Immobilization Original Article Peroxidase Phenols Phenolsulfonphthalein - chemistry Starch Starch - chemistry Starch - metabolism Temperature Thermal stability
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