Adaptable, turn-on maturation (ATOM) fluorescent biosensors for multiplexed detection in cells

A grand challenge in biosensor design is to develop a single-molecule, fluorescent protein-based platform that can be easily adapted to recognize targets of choice. Here, we created a family of adaptable, turn-on maturation (ATOM) biosensors consisting of a monobody (circularly permuted at one of tw...

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Veröffentlicht in:	Nature methods 2023-12, Vol.20 (12), p.1920-1929
Hauptverfasser:	Sekhon, Harsimranjit, Ha, Jeung-Hoi, Presti, Maria F., Procopio, Spencer B., Jarvis, Ava R., Mirsky, Paige O., John, Anna M., Loh, Stewart N.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1888/2249 631/61/338/469 631/61/350/59 Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomolecules Biosensing Techniques - methods Biosensors Chromophores Endoplasmic reticulum Fluorescence Fluorescence Resonance Energy Transfer - methods Fluorescent Dyes - metabolism Humans Life Sciences Ligands Maturation Multiplexing Nanobodies Organelles Proteins Proteomics Sensors Target detection Target recognition
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