A murine monoclonal antibody against H5N1 avian influenza virus cross-reacts with human kidney cortex cells
To investigate the biological characteristics of monoclonal antibodies (mAbs) against avian influenza virus (AIV) and the possible mechanism of AIV-related kidney injury. BALB/c mice were immunized with inactivated H5N1 AIV to prepare monoclonal antibody H5-32, and its subtype, titer and cross-react...
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| Veröffentlicht in: | Archives of microbiology 2023-11, Vol.205 (12), p.373-373, Article 373 |
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| creator | Zhang, Haixiang Sun, Jingying Feng, Yangmeng Li, Jingli Wang, Nana Zhao, Xiangrong Li, Yan Hu, Jun Sun, Lijun Xu, Cuixiang |
| description | To investigate the biological characteristics of monoclonal antibodies (mAbs) against avian influenza virus (AIV) and the possible mechanism of AIV-related kidney injury. BALB/c mice were immunized with inactivated H5N1 AIV to prepare monoclonal antibody H5-32, and its subtype, titer and cross-reactivity with other influenza viruses were identified. The reactivity of monoclonal antibody with normal human tissue was analyzed by immunohistochemistry. Immunofluorescence and confocal laser scanning technique were used to detect the binding sites between mAb and human renal cortical cells, and Western blotting was used to detect the size of binding fragments. Immunohistochemical analysis confirmed that monoclonal antibody H5-32 cross-reacted with normal human kidney tissue. In human kidney, mAb H5-32 was localized in the cytoplasm of human renal tubular epithelial cells, and its binding fragment size was about 43 kDa. H5N1 AIV appears to bind to human renal tubular epithelial cells, which may be one of the mechanisms of kidney injury caused by AIV infection. |
| doi_str_mv | 10.1007/s00203-023-03693-8 |
| format | Article |
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BALB/c mice were immunized with inactivated H5N1 AIV to prepare monoclonal antibody H5-32, and its subtype, titer and cross-reactivity with other influenza viruses were identified. The reactivity of monoclonal antibody with normal human tissue was analyzed by immunohistochemistry. Immunofluorescence and confocal laser scanning technique were used to detect the binding sites between mAb and human renal cortical cells, and Western blotting was used to detect the size of binding fragments. Immunohistochemical analysis confirmed that monoclonal antibody H5-32 cross-reacted with normal human kidney tissue. In human kidney, mAb H5-32 was localized in the cytoplasm of human renal tubular epithelial cells, and its binding fragment size was about 43 kDa. H5N1 AIV appears to bind to human renal tubular epithelial cells, which may be one of the mechanisms of kidney injury caused by AIV infection.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-023-03693-8</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antigens ; Archives & records ; Avian flu ; Binding sites ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Cross-reactivity ; Cytoplasm ; Ecology ; Edema ; Epithelial cells ; Epithelium ; Human tissues ; Immunization ; Immunofluorescence ; Immunohistochemistry ; Infections ; Influenza ; Kidneys ; Life Sciences ; Microbial Ecology ; Microbiology ; Monoclonal antibodies ; Original Paper ; Renal cortex ; Viruses ; Western blotting</subject><ispartof>Archives of microbiology, 2023-11, Vol.205 (12), p.373-373, Article 373</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c331t-304a69308808bb453bf490b8912989cfc0d1bbc61dce9925b6ae2dc7a33c8af53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-023-03693-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-023-03693-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Zhang, Haixiang</creatorcontrib><creatorcontrib>Sun, Jingying</creatorcontrib><creatorcontrib>Feng, Yangmeng</creatorcontrib><creatorcontrib>Li, Jingli</creatorcontrib><creatorcontrib>Wang, Nana</creatorcontrib><creatorcontrib>Zhao, Xiangrong</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Hu, Jun</creatorcontrib><creatorcontrib>Sun, Lijun</creatorcontrib><creatorcontrib>Xu, Cuixiang</creatorcontrib><title>A murine monoclonal antibody against H5N1 avian influenza virus cross-reacts with human kidney cortex cells</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><description>To investigate the biological characteristics of monoclonal antibodies (mAbs) against avian influenza virus (AIV) and the possible mechanism of AIV-related kidney injury. BALB/c mice were immunized with inactivated H5N1 AIV to prepare monoclonal antibody H5-32, and its subtype, titer and cross-reactivity with other influenza viruses were identified. The reactivity of monoclonal antibody with normal human tissue was analyzed by immunohistochemistry. Immunofluorescence and confocal laser scanning technique were used to detect the binding sites between mAb and human renal cortical cells, and Western blotting was used to detect the size of binding fragments. Immunohistochemical analysis confirmed that monoclonal antibody H5-32 cross-reacted with normal human kidney tissue. In human kidney, mAb H5-32 was localized in the cytoplasm of human renal tubular epithelial cells, and its binding fragment size was about 43 kDa. H5N1 AIV appears to bind to human renal tubular epithelial cells, which may be one of the mechanisms of kidney injury caused by AIV infection.</description><subject>Antigens</subject><subject>Archives & records</subject><subject>Avian flu</subject><subject>Binding sites</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Cross-reactivity</subject><subject>Cytoplasm</subject><subject>Ecology</subject><subject>Edema</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Human tissues</subject><subject>Immunization</subject><subject>Immunofluorescence</subject><subject>Immunohistochemistry</subject><subject>Infections</subject><subject>Influenza</subject><subject>Kidneys</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Monoclonal antibodies</subject><subject>Original 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Haixiang ; Sun, Jingying ; Feng, Yangmeng ; Li, Jingli ; Wang, Nana ; Zhao, Xiangrong ; Li, Yan ; Hu, Jun ; Sun, Lijun ; Xu, Cuixiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-304a69308808bb453bf490b8912989cfc0d1bbc61dce9925b6ae2dc7a33c8af53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antigens</topic><topic>Archives & records</topic><topic>Avian flu</topic><topic>Binding sites</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Cross-reactivity</topic><topic>Cytoplasm</topic><topic>Ecology</topic><topic>Edema</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Human 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Microbiol</stitle><date>2023-11-01</date><risdate>2023</risdate><volume>205</volume><issue>12</issue><spage>373</spage><epage>373</epage><pages>373-373</pages><artnum>373</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>To investigate the biological characteristics of monoclonal antibodies (mAbs) against avian influenza virus (AIV) and the possible mechanism of AIV-related kidney injury. BALB/c mice were immunized with inactivated H5N1 AIV to prepare monoclonal antibody H5-32, and its subtype, titer and cross-reactivity with other influenza viruses were identified. The reactivity of monoclonal antibody with normal human tissue was analyzed by immunohistochemistry. Immunofluorescence and confocal laser scanning technique were used to detect the binding sites between mAb and human renal cortical cells, and Western blotting was used to detect the size of binding fragments. Immunohistochemical analysis confirmed that monoclonal antibody H5-32 cross-reacted with normal human kidney tissue. In human kidney, mAb H5-32 was localized in the cytoplasm of human renal tubular epithelial cells, and its binding fragment size was about 43 kDa. H5N1 AIV appears to bind to human renal tubular epithelial cells, which may be one of the mechanisms of kidney injury caused by AIV infection.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00203-023-03693-8</doi><tpages>1</tpages></addata></record> |
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| subjects | Antigens Archives & records Avian flu Binding sites Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Cross-reactivity Cytoplasm Ecology Edema Epithelial cells Epithelium Human tissues Immunization Immunofluorescence Immunohistochemistry Infections Influenza Kidneys Life Sciences Microbial Ecology Microbiology Monoclonal antibodies Original Paper Renal cortex Viruses Western blotting |
| title | A murine monoclonal antibody against H5N1 avian influenza virus cross-reacts with human kidney cortex cells |
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