Growth differentiation factor-15 stimulates the synthesis of corticotropin-releasing factor in hypothalamic 4B cells
Growth differentiation factor-15 (GDF15) is a stress-activated cytokine that regulates cell growth and inflammatory and stress responses. We previously reported the role and regulation of GDF15 in pituitary corticotrophs. Dexamethasone increases Gdf15 gene expression levels and production. GDF15 sup...
Gespeichert in:
Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2023-12, Vol.170, p.171112-171112, Article 171112 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 171112 |
---|---|
container_issue | |
container_start_page | 171112 |
container_title | Peptides (New York, N.Y. : 1980) |
container_volume | 170 |
creator | Tasso, Mizuki Kageyama, Kazunori Iwasaki, Yasumasa Watanuki, Yutaka Niioka, Kanako Takayasu, Shinobu Daimon, Makoto |
description | Growth differentiation factor-15 (GDF15) is a stress-activated cytokine that regulates cell growth and inflammatory and stress responses. We previously reported the role and regulation of GDF15 in pituitary corticotrophs. Dexamethasone increases Gdf15 gene expression levels and production. GDF15 suppresses adrenocorticotropic hormone synthesis in pituitary corticotrophs and subsequently mediates the negative feedback effect of glucocorticoids. Here, we analyzed corticotropin-releasing factor (Crf) promoter activity in hypothalamic 4B cells transfected with promoter-driven luciferase reporter constructs. The effects of time and GDF15 concentration on Crf mRNA levels were analyzed using quantitative real-time polymerase chain reaction. Glial cell-derived neurotrophic factor family receptor α-like (GFRAL) protein is expressed in 4B cells. GDF15 increased Crf promoter activity and Crf mRNA levels in 4B cells. The protein kinase A and C pathways also contributed to the GDF15-induced increase in Crf gene expression. GDF15 stimulates GFRAL, subsequently increasing the phosphorylation of AKT, an extracellular signal-related kinase, and the cAMP response element-binding protein. Therefore, GDF15-dependent pathways may be involved in regulating Crf expression under stressful conditions in hypothalamic cells. |
doi_str_mv | 10.1016/j.peptides.2023.171112 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2886331020</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2886331020</sourcerecordid><originalsourceid>FETCH-LOGICAL-c291t-5c5f11afd87fb4518d86c295e4dcf87b533f4092948dbc252a62053771b50c5e3</originalsourceid><addsrcrecordid>eNqFkctOwzAQRS0EgvL4BeQlmxSPH4mzhAoKEhIbWFuOY1NXSRxsR6h_T6u2bFndxdwzM9JB6BbIHAiU9-v5aMfsW5vmlFA2hwoA6AmagaxYIaCsT9GMQF0WdSXhAl2mtCaEcF7Lc3TBqhokl3yG8jKGn7zCrXfORjtkr7MPA3ba5BALEDhl30-dzjbhvLI4bYZtJJ9wcNiEmL0JOYbRD0W0ndXJD18HGvsBrzZjyCvd6d4bzB-xsV2XrtGZ012yN4e8Qp_PTx-Ll-Ltffm6eHgrDK0hF8IIB6BdKyvXcAGyleV2IixvjZNVIxhznNS05rJtDBVUl5QIVlXQCGKEZVfobr93jOF7simr3qfdB3qwYUqKgeAgS0npv1UqZckYEEq21XJfNTGkFK1TY_S9jhsFRO3kqLU6ylE7OWovZwveHm5MTW_bP-xog_0CsmKPNg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2886331020</pqid></control><display><type>article</type><title>Growth differentiation factor-15 stimulates the synthesis of corticotropin-releasing factor in hypothalamic 4B cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Tasso, Mizuki ; Kageyama, Kazunori ; Iwasaki, Yasumasa ; Watanuki, Yutaka ; Niioka, Kanako ; Takayasu, Shinobu ; Daimon, Makoto</creator><creatorcontrib>Tasso, Mizuki ; Kageyama, Kazunori ; Iwasaki, Yasumasa ; Watanuki, Yutaka ; Niioka, Kanako ; Takayasu, Shinobu ; Daimon, Makoto</creatorcontrib><description>Growth differentiation factor-15 (GDF15) is a stress-activated cytokine that regulates cell growth and inflammatory and stress responses. We previously reported the role and regulation of GDF15 in pituitary corticotrophs. Dexamethasone increases Gdf15 gene expression levels and production. GDF15 suppresses adrenocorticotropic hormone synthesis in pituitary corticotrophs and subsequently mediates the negative feedback effect of glucocorticoids. Here, we analyzed corticotropin-releasing factor (Crf) promoter activity in hypothalamic 4B cells transfected with promoter-driven luciferase reporter constructs. The effects of time and GDF15 concentration on Crf mRNA levels were analyzed using quantitative real-time polymerase chain reaction. Glial cell-derived neurotrophic factor family receptor α-like (GFRAL) protein is expressed in 4B cells. GDF15 increased Crf promoter activity and Crf mRNA levels in 4B cells. The protein kinase A and C pathways also contributed to the GDF15-induced increase in Crf gene expression. GDF15 stimulates GFRAL, subsequently increasing the phosphorylation of AKT, an extracellular signal-related kinase, and the cAMP response element-binding protein. Therefore, GDF15-dependent pathways may be involved in regulating Crf expression under stressful conditions in hypothalamic cells.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/j.peptides.2023.171112</identifier><identifier>PMID: 37918484</identifier><language>eng</language><publisher>United States</publisher><subject>cAMP-dependent protein kinase ; cell growth ; corticotropin ; corticotropin-releasing hormone ; Corticotropin-Releasing Hormone - genetics ; Corticotropin-Releasing Hormone - metabolism ; cytokines ; dexamethasone ; family ; gene expression ; glucocorticoids ; Growth Differentiation Factors - genetics ; Growth Differentiation Factors - metabolism ; Growth Differentiation Factors - pharmacology ; Hypothalamus - metabolism ; luciferase ; phosphorylation ; Promoter Regions, Genetic ; quantitative polymerase chain reaction ; Receptors, Corticotropin-Releasing Hormone - genetics ; Receptors, Corticotropin-Releasing Hormone - metabolism ; RNA, Messenger - metabolism</subject><ispartof>Peptides (New York, N.Y. : 1980), 2023-12, Vol.170, p.171112-171112, Article 171112</ispartof><rights>Copyright © 2023 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c291t-5c5f11afd87fb4518d86c295e4dcf87b533f4092948dbc252a62053771b50c5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37918484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tasso, Mizuki</creatorcontrib><creatorcontrib>Kageyama, Kazunori</creatorcontrib><creatorcontrib>Iwasaki, Yasumasa</creatorcontrib><creatorcontrib>Watanuki, Yutaka</creatorcontrib><creatorcontrib>Niioka, Kanako</creatorcontrib><creatorcontrib>Takayasu, Shinobu</creatorcontrib><creatorcontrib>Daimon, Makoto</creatorcontrib><title>Growth differentiation factor-15 stimulates the synthesis of corticotropin-releasing factor in hypothalamic 4B cells</title><title>Peptides (New York, N.Y. : 1980)</title><addtitle>Peptides</addtitle><description>Growth differentiation factor-15 (GDF15) is a stress-activated cytokine that regulates cell growth and inflammatory and stress responses. We previously reported the role and regulation of GDF15 in pituitary corticotrophs. Dexamethasone increases Gdf15 gene expression levels and production. GDF15 suppresses adrenocorticotropic hormone synthesis in pituitary corticotrophs and subsequently mediates the negative feedback effect of glucocorticoids. Here, we analyzed corticotropin-releasing factor (Crf) promoter activity in hypothalamic 4B cells transfected with promoter-driven luciferase reporter constructs. The effects of time and GDF15 concentration on Crf mRNA levels were analyzed using quantitative real-time polymerase chain reaction. Glial cell-derived neurotrophic factor family receptor α-like (GFRAL) protein is expressed in 4B cells. GDF15 increased Crf promoter activity and Crf mRNA levels in 4B cells. The protein kinase A and C pathways also contributed to the GDF15-induced increase in Crf gene expression. GDF15 stimulates GFRAL, subsequently increasing the phosphorylation of AKT, an extracellular signal-related kinase, and the cAMP response element-binding protein. Therefore, GDF15-dependent pathways may be involved in regulating Crf expression under stressful conditions in hypothalamic cells.</description><subject>cAMP-dependent protein kinase</subject><subject>cell growth</subject><subject>corticotropin</subject><subject>corticotropin-releasing hormone</subject><subject>Corticotropin-Releasing Hormone - genetics</subject><subject>Corticotropin-Releasing Hormone - metabolism</subject><subject>cytokines</subject><subject>dexamethasone</subject><subject>family</subject><subject>gene expression</subject><subject>glucocorticoids</subject><subject>Growth Differentiation Factors - genetics</subject><subject>Growth Differentiation Factors - metabolism</subject><subject>Growth Differentiation Factors - pharmacology</subject><subject>Hypothalamus - metabolism</subject><subject>luciferase</subject><subject>phosphorylation</subject><subject>Promoter Regions, Genetic</subject><subject>quantitative polymerase chain reaction</subject><subject>Receptors, Corticotropin-Releasing Hormone - genetics</subject><subject>Receptors, Corticotropin-Releasing Hormone - metabolism</subject><subject>RNA, Messenger - metabolism</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctOwzAQRS0EgvL4BeQlmxSPH4mzhAoKEhIbWFuOY1NXSRxsR6h_T6u2bFndxdwzM9JB6BbIHAiU9-v5aMfsW5vmlFA2hwoA6AmagaxYIaCsT9GMQF0WdSXhAl2mtCaEcF7Lc3TBqhokl3yG8jKGn7zCrXfORjtkr7MPA3ba5BALEDhl30-dzjbhvLI4bYZtJJ9wcNiEmL0JOYbRD0W0ndXJD18HGvsBrzZjyCvd6d4bzB-xsV2XrtGZ012yN4e8Qp_PTx-Ll-Ltffm6eHgrDK0hF8IIB6BdKyvXcAGyleV2IixvjZNVIxhznNS05rJtDBVUl5QIVlXQCGKEZVfobr93jOF7simr3qfdB3qwYUqKgeAgS0npv1UqZckYEEq21XJfNTGkFK1TY_S9jhsFRO3kqLU6ylE7OWovZwveHm5MTW_bP-xog_0CsmKPNg</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Tasso, Mizuki</creator><creator>Kageyama, Kazunori</creator><creator>Iwasaki, Yasumasa</creator><creator>Watanuki, Yutaka</creator><creator>Niioka, Kanako</creator><creator>Takayasu, Shinobu</creator><creator>Daimon, Makoto</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202312</creationdate><title>Growth differentiation factor-15 stimulates the synthesis of corticotropin-releasing factor in hypothalamic 4B cells</title><author>Tasso, Mizuki ; Kageyama, Kazunori ; Iwasaki, Yasumasa ; Watanuki, Yutaka ; Niioka, Kanako ; Takayasu, Shinobu ; Daimon, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c291t-5c5f11afd87fb4518d86c295e4dcf87b533f4092948dbc252a62053771b50c5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>cAMP-dependent protein kinase</topic><topic>cell growth</topic><topic>corticotropin</topic><topic>corticotropin-releasing hormone</topic><topic>Corticotropin-Releasing Hormone - genetics</topic><topic>Corticotropin-Releasing Hormone - metabolism</topic><topic>cytokines</topic><topic>dexamethasone</topic><topic>family</topic><topic>gene expression</topic><topic>glucocorticoids</topic><topic>Growth Differentiation Factors - genetics</topic><topic>Growth Differentiation Factors - metabolism</topic><topic>Growth Differentiation Factors - pharmacology</topic><topic>Hypothalamus - metabolism</topic><topic>luciferase</topic><topic>phosphorylation</topic><topic>Promoter Regions, Genetic</topic><topic>quantitative polymerase chain reaction</topic><topic>Receptors, Corticotropin-Releasing Hormone - genetics</topic><topic>Receptors, Corticotropin-Releasing Hormone - metabolism</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tasso, Mizuki</creatorcontrib><creatorcontrib>Kageyama, Kazunori</creatorcontrib><creatorcontrib>Iwasaki, Yasumasa</creatorcontrib><creatorcontrib>Watanuki, Yutaka</creatorcontrib><creatorcontrib>Niioka, Kanako</creatorcontrib><creatorcontrib>Takayasu, Shinobu</creatorcontrib><creatorcontrib>Daimon, Makoto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tasso, Mizuki</au><au>Kageyama, Kazunori</au><au>Iwasaki, Yasumasa</au><au>Watanuki, Yutaka</au><au>Niioka, Kanako</au><au>Takayasu, Shinobu</au><au>Daimon, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth differentiation factor-15 stimulates the synthesis of corticotropin-releasing factor in hypothalamic 4B cells</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>2023-12</date><risdate>2023</risdate><volume>170</volume><spage>171112</spage><epage>171112</epage><pages>171112-171112</pages><artnum>171112</artnum><issn>0196-9781</issn><eissn>1873-5169</eissn><abstract>Growth differentiation factor-15 (GDF15) is a stress-activated cytokine that regulates cell growth and inflammatory and stress responses. We previously reported the role and regulation of GDF15 in pituitary corticotrophs. Dexamethasone increases Gdf15 gene expression levels and production. GDF15 suppresses adrenocorticotropic hormone synthesis in pituitary corticotrophs and subsequently mediates the negative feedback effect of glucocorticoids. Here, we analyzed corticotropin-releasing factor (Crf) promoter activity in hypothalamic 4B cells transfected with promoter-driven luciferase reporter constructs. The effects of time and GDF15 concentration on Crf mRNA levels were analyzed using quantitative real-time polymerase chain reaction. Glial cell-derived neurotrophic factor family receptor α-like (GFRAL) protein is expressed in 4B cells. GDF15 increased Crf promoter activity and Crf mRNA levels in 4B cells. The protein kinase A and C pathways also contributed to the GDF15-induced increase in Crf gene expression. GDF15 stimulates GFRAL, subsequently increasing the phosphorylation of AKT, an extracellular signal-related kinase, and the cAMP response element-binding protein. Therefore, GDF15-dependent pathways may be involved in regulating Crf expression under stressful conditions in hypothalamic cells.</abstract><cop>United States</cop><pmid>37918484</pmid><doi>10.1016/j.peptides.2023.171112</doi><tpages>1</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0196-9781 |
ispartof | Peptides (New York, N.Y. : 1980), 2023-12, Vol.170, p.171112-171112, Article 171112 |
issn | 0196-9781 1873-5169 |
language | eng |
recordid | cdi_proquest_miscellaneous_2886331020 |
source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | cAMP-dependent protein kinase cell growth corticotropin corticotropin-releasing hormone Corticotropin-Releasing Hormone - genetics Corticotropin-Releasing Hormone - metabolism cytokines dexamethasone family gene expression glucocorticoids Growth Differentiation Factors - genetics Growth Differentiation Factors - metabolism Growth Differentiation Factors - pharmacology Hypothalamus - metabolism luciferase phosphorylation Promoter Regions, Genetic quantitative polymerase chain reaction Receptors, Corticotropin-Releasing Hormone - genetics Receptors, Corticotropin-Releasing Hormone - metabolism RNA, Messenger - metabolism |
title | Growth differentiation factor-15 stimulates the synthesis of corticotropin-releasing factor in hypothalamic 4B cells |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T00%3A51%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Growth%20differentiation%20factor-15%20stimulates%20the%20synthesis%20of%20corticotropin-releasing%20factor%20in%20hypothalamic%204B%20cells&rft.jtitle=Peptides%20(New%20York,%20N.Y.%20:%201980)&rft.au=Tasso,%20Mizuki&rft.date=2023-12&rft.volume=170&rft.spage=171112&rft.epage=171112&rft.pages=171112-171112&rft.artnum=171112&rft.issn=0196-9781&rft.eissn=1873-5169&rft_id=info:doi/10.1016/j.peptides.2023.171112&rft_dat=%3Cproquest_cross%3E2886331020%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2886331020&rft_id=info:pmid/37918484&rfr_iscdi=true |