Sprayable chitosan nanogel with nitric oxide to accelerate diabetic wound healing through bacteria inhibition, biofilm eradication and macrophage polarization

Bacterial infection and chronic inflammation are two major risks in diabetic wound healing, which increase patient mortality. In this study, a multifunctional sprayable nanogel (Ag-G@CS) based on chitosan has been developed to synergistically inhibit bacterial infection, eradicate biofilm, and relie...

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Veröffentlicht in:International journal of biological macromolecules 2024-01, Vol.254 (Pt 1), p.127806-127806, Article 127806
Hauptverfasser: Huang, Qinqin, Yang, Zheng, Tao, Xinyue, Ma, Chenyu, Cao, Peiyao, Wei, Ping, Jiang, Chenxiao, Ren, Hao, Li, Xueming
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container_end_page 127806
container_issue Pt 1
container_start_page 127806
container_title International journal of biological macromolecules
container_volume 254
creator Huang, Qinqin
Yang, Zheng
Tao, Xinyue
Ma, Chenyu
Cao, Peiyao
Wei, Ping
Jiang, Chenxiao
Ren, Hao
Li, Xueming
description Bacterial infection and chronic inflammation are two major risks in diabetic wound healing, which increase patient mortality. In this study, a multifunctional sprayable nanogel (Ag-G@CS) based on chitosan has been developed to synergistically inhibit bacterial infection, eradicate biofilm, and relieve inflammation of diabetic wounds. The nanogel is successfully crafted by encapsulating with a nitric oxide (NO) donor and performing in-situ reduction of silver nanoparticles (Ag). The released NO enhances the antibacterial efficacy of Ag, nearly achieving complete eradication of biofilms in vitro. Upon application on both normal or diabetic chronic wounds, the combination effects of released NO and Ag offer a notable antibacterial effect. Furthermore, after bacteria inhibition and biofilm eradication, the NO released by the nanogel orchestrates a transformation of M1 macrophages into M2 macrophages, significantly reducing tumor necrosis factor α (TNF-α) release and relieving inflammation. Remarkably, the released NO also promotes M2a to M2c macrophages, thereby facilitating tissue remodeling in chronic wounds. More importantly, it upregulates the expression of vascular endothelial growth factor (VEGF), further accelerating the wound healing process. Collectively, the formed sprayable nanogel exhibits excellent inhibition of bacterial infections and biofilms, and promotes chronic wound healing via inflammation resolution, which has excellent potential for clinical use in the future.
doi_str_mv 10.1016/j.ijbiomac.2023.127806
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In this study, a multifunctional sprayable nanogel (Ag-G@CS) based on chitosan has been developed to synergistically inhibit bacterial infection, eradicate biofilm, and relieve inflammation of diabetic wounds. The nanogel is successfully crafted by encapsulating with a nitric oxide (NO) donor and performing in-situ reduction of silver nanoparticles (Ag). The released NO enhances the antibacterial efficacy of Ag, nearly achieving complete eradication of biofilms in vitro. Upon application on both normal or diabetic chronic wounds, the combination effects of released NO and Ag offer a notable antibacterial effect. Furthermore, after bacteria inhibition and biofilm eradication, the NO released by the nanogel orchestrates a transformation of M1 macrophages into M2 macrophages, significantly reducing tumor necrosis factor α (TNF-α) release and relieving inflammation. Remarkably, the released NO also promotes M2a to M2c macrophages, thereby facilitating tissue remodeling in chronic wounds. More importantly, it upregulates the expression of vascular endothelial growth factor (VEGF), further accelerating the wound healing process. 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subjects Animals
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Bacteria
Bacterial Infections
Biofilm eradication
Biofilms
Chitosan - pharmacology
Diabetes Mellitus, Experimental - metabolism
Diabetic wound healing
Humans
Inflammation
Macrophages
Macrophages polarization
Metal Nanoparticles
Nanogels
Nitric oxide
Nitric Oxide - pharmacology
Silver - pharmacology
Vascular Endothelial Growth Factor A - pharmacology
Wound Healing
title Sprayable chitosan nanogel with nitric oxide to accelerate diabetic wound healing through bacteria inhibition, biofilm eradication and macrophage polarization
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