Sprayable chitosan nanogel with nitric oxide to accelerate diabetic wound healing through bacteria inhibition, biofilm eradication and macrophage polarization
Bacterial infection and chronic inflammation are two major risks in diabetic wound healing, which increase patient mortality. In this study, a multifunctional sprayable nanogel (Ag-G@CS) based on chitosan has been developed to synergistically inhibit bacterial infection, eradicate biofilm, and relie...
Gespeichert in:
Veröffentlicht in: | International journal of biological macromolecules 2024-01, Vol.254 (Pt 1), p.127806-127806, Article 127806 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 127806 |
---|---|
container_issue | Pt 1 |
container_start_page | 127806 |
container_title | International journal of biological macromolecules |
container_volume | 254 |
creator | Huang, Qinqin Yang, Zheng Tao, Xinyue Ma, Chenyu Cao, Peiyao Wei, Ping Jiang, Chenxiao Ren, Hao Li, Xueming |
description | Bacterial infection and chronic inflammation are two major risks in diabetic wound healing, which increase patient mortality. In this study, a multifunctional sprayable nanogel (Ag-G@CS) based on chitosan has been developed to synergistically inhibit bacterial infection, eradicate biofilm, and relieve inflammation of diabetic wounds. The nanogel is successfully crafted by encapsulating with a nitric oxide (NO) donor and performing in-situ reduction of silver nanoparticles (Ag). The released NO enhances the antibacterial efficacy of Ag, nearly achieving complete eradication of biofilms in vitro. Upon application on both normal or diabetic chronic wounds, the combination effects of released NO and Ag offer a notable antibacterial effect. Furthermore, after bacteria inhibition and biofilm eradication, the NO released by the nanogel orchestrates a transformation of M1 macrophages into M2 macrophages, significantly reducing tumor necrosis factor α (TNF-α) release and relieving inflammation. Remarkably, the released NO also promotes M2a to M2c macrophages, thereby facilitating tissue remodeling in chronic wounds. More importantly, it upregulates the expression of vascular endothelial growth factor (VEGF), further accelerating the wound healing process. Collectively, the formed sprayable nanogel exhibits excellent inhibition of bacterial infections and biofilms, and promotes chronic wound healing via inflammation resolution, which has excellent potential for clinical use in the future. |
doi_str_mv | 10.1016/j.ijbiomac.2023.127806 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2886331002</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0141813023047050</els_id><sourcerecordid>2886331002</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-4ab747a8c068954c8c1d57bfd06aae2f74da97145da1e069da851bffda8492ce3</originalsourceid><addsrcrecordid>eNqFUctuFDEQtBCIbBZ-IfKRA7PY8_TcQBGPSJE4AGerx-7Z6dWMPdheQvgYvhUvm3Dl1FJ3VXdVF2NXUuykkO2bw44OA_kFzK4UZbWTZadE-4RtpOr6QghRPWUbIWtZKFmJC3YZ4yF320aq5-yi6nqpmr7asN9f1gD3MMzIzUTJR3DcgfN7nPkdpYk7SoEM9z_JIk-egzE4Y4CE3BIMmPLwzh-d5RPCTG7P0xT8cT_xAUzCQMDJTTRQIu9e86x5pHnheYMlA6cmh0zORoJfJ9gjX_0MgX79nb1gz0aYI758qFv27cP7r9efitvPH2-u390WpmpVKmoYuroDZUSr-qY2ykjbdMNoRQuA5djVFvpO1o0FiaLtLahGDuOYa92XBqste3Xeuwb__Ygx6YViNjqDQ3-MulSqrSop8qu3rD1Ds-AYA456DbRAuNdS6FM2-qAfs9GnbPQ5m0y8erhxHBa0_2iPYWTA2zMAs9MfhEFHQ-gMWgpokrae_nfjD3p-qF8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2886331002</pqid></control><display><type>article</type><title>Sprayable chitosan nanogel with nitric oxide to accelerate diabetic wound healing through bacteria inhibition, biofilm eradication and macrophage polarization</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Huang, Qinqin ; Yang, Zheng ; Tao, Xinyue ; Ma, Chenyu ; Cao, Peiyao ; Wei, Ping ; Jiang, Chenxiao ; Ren, Hao ; Li, Xueming</creator><creatorcontrib>Huang, Qinqin ; Yang, Zheng ; Tao, Xinyue ; Ma, Chenyu ; Cao, Peiyao ; Wei, Ping ; Jiang, Chenxiao ; Ren, Hao ; Li, Xueming</creatorcontrib><description>Bacterial infection and chronic inflammation are two major risks in diabetic wound healing, which increase patient mortality. In this study, a multifunctional sprayable nanogel (Ag-G@CS) based on chitosan has been developed to synergistically inhibit bacterial infection, eradicate biofilm, and relieve inflammation of diabetic wounds. The nanogel is successfully crafted by encapsulating with a nitric oxide (NO) donor and performing in-situ reduction of silver nanoparticles (Ag). The released NO enhances the antibacterial efficacy of Ag, nearly achieving complete eradication of biofilms in vitro. Upon application on both normal or diabetic chronic wounds, the combination effects of released NO and Ag offer a notable antibacterial effect. Furthermore, after bacteria inhibition and biofilm eradication, the NO released by the nanogel orchestrates a transformation of M1 macrophages into M2 macrophages, significantly reducing tumor necrosis factor α (TNF-α) release and relieving inflammation. Remarkably, the released NO also promotes M2a to M2c macrophages, thereby facilitating tissue remodeling in chronic wounds. More importantly, it upregulates the expression of vascular endothelial growth factor (VEGF), further accelerating the wound healing process. Collectively, the formed sprayable nanogel exhibits excellent inhibition of bacterial infections and biofilms, and promotes chronic wound healing via inflammation resolution, which has excellent potential for clinical use in the future.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2023.127806</identifier><identifier>PMID: 37918593</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibacterial activity ; Bacteria ; Bacterial Infections ; Biofilm eradication ; Biofilms ; Chitosan - pharmacology ; Diabetes Mellitus, Experimental - metabolism ; Diabetic wound healing ; Humans ; Inflammation ; Macrophages ; Macrophages polarization ; Metal Nanoparticles ; Nanogels ; Nitric oxide ; Nitric Oxide - pharmacology ; Silver - pharmacology ; Vascular Endothelial Growth Factor A - pharmacology ; Wound Healing</subject><ispartof>International journal of biological macromolecules, 2024-01, Vol.254 (Pt 1), p.127806-127806, Article 127806</ispartof><rights>2023 Elsevier B.V.</rights><rights>Copyright © 2023 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-4ab747a8c068954c8c1d57bfd06aae2f74da97145da1e069da851bffda8492ce3</citedby><cites>FETCH-LOGICAL-c368t-4ab747a8c068954c8c1d57bfd06aae2f74da97145da1e069da851bffda8492ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2023.127806$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37918593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Qinqin</creatorcontrib><creatorcontrib>Yang, Zheng</creatorcontrib><creatorcontrib>Tao, Xinyue</creatorcontrib><creatorcontrib>Ma, Chenyu</creatorcontrib><creatorcontrib>Cao, Peiyao</creatorcontrib><creatorcontrib>Wei, Ping</creatorcontrib><creatorcontrib>Jiang, Chenxiao</creatorcontrib><creatorcontrib>Ren, Hao</creatorcontrib><creatorcontrib>Li, Xueming</creatorcontrib><title>Sprayable chitosan nanogel with nitric oxide to accelerate diabetic wound healing through bacteria inhibition, biofilm eradication and macrophage polarization</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Bacterial infection and chronic inflammation are two major risks in diabetic wound healing, which increase patient mortality. In this study, a multifunctional sprayable nanogel (Ag-G@CS) based on chitosan has been developed to synergistically inhibit bacterial infection, eradicate biofilm, and relieve inflammation of diabetic wounds. The nanogel is successfully crafted by encapsulating with a nitric oxide (NO) donor and performing in-situ reduction of silver nanoparticles (Ag). The released NO enhances the antibacterial efficacy of Ag, nearly achieving complete eradication of biofilms in vitro. Upon application on both normal or diabetic chronic wounds, the combination effects of released NO and Ag offer a notable antibacterial effect. Furthermore, after bacteria inhibition and biofilm eradication, the NO released by the nanogel orchestrates a transformation of M1 macrophages into M2 macrophages, significantly reducing tumor necrosis factor α (TNF-α) release and relieving inflammation. Remarkably, the released NO also promotes M2a to M2c macrophages, thereby facilitating tissue remodeling in chronic wounds. More importantly, it upregulates the expression of vascular endothelial growth factor (VEGF), further accelerating the wound healing process. Collectively, the formed sprayable nanogel exhibits excellent inhibition of bacterial infections and biofilms, and promotes chronic wound healing via inflammation resolution, which has excellent potential for clinical use in the future.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial activity</subject><subject>Bacteria</subject><subject>Bacterial Infections</subject><subject>Biofilm eradication</subject><subject>Biofilms</subject><subject>Chitosan - pharmacology</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetic wound healing</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Macrophages</subject><subject>Macrophages polarization</subject><subject>Metal Nanoparticles</subject><subject>Nanogels</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - pharmacology</subject><subject>Silver - pharmacology</subject><subject>Vascular Endothelial Growth Factor A - pharmacology</subject><subject>Wound Healing</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctuFDEQtBCIbBZ-IfKRA7PY8_TcQBGPSJE4AGerx-7Z6dWMPdheQvgYvhUvm3Dl1FJ3VXdVF2NXUuykkO2bw44OA_kFzK4UZbWTZadE-4RtpOr6QghRPWUbIWtZKFmJC3YZ4yF320aq5-yi6nqpmr7asN9f1gD3MMzIzUTJR3DcgfN7nPkdpYk7SoEM9z_JIk-egzE4Y4CE3BIMmPLwzh-d5RPCTG7P0xT8cT_xAUzCQMDJTTRQIu9e86x5pHnheYMlA6cmh0zORoJfJ9gjX_0MgX79nb1gz0aYI758qFv27cP7r9efitvPH2-u390WpmpVKmoYuroDZUSr-qY2ykjbdMNoRQuA5djVFvpO1o0FiaLtLahGDuOYa92XBqste3Xeuwb__Ygx6YViNjqDQ3-MulSqrSop8qu3rD1Ds-AYA456DbRAuNdS6FM2-qAfs9GnbPQ5m0y8erhxHBa0_2iPYWTA2zMAs9MfhEFHQ-gMWgpokrae_nfjD3p-qF8</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Huang, Qinqin</creator><creator>Yang, Zheng</creator><creator>Tao, Xinyue</creator><creator>Ma, Chenyu</creator><creator>Cao, Peiyao</creator><creator>Wei, Ping</creator><creator>Jiang, Chenxiao</creator><creator>Ren, Hao</creator><creator>Li, Xueming</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202401</creationdate><title>Sprayable chitosan nanogel with nitric oxide to accelerate diabetic wound healing through bacteria inhibition, biofilm eradication and macrophage polarization</title><author>Huang, Qinqin ; Yang, Zheng ; Tao, Xinyue ; Ma, Chenyu ; Cao, Peiyao ; Wei, Ping ; Jiang, Chenxiao ; Ren, Hao ; Li, Xueming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-4ab747a8c068954c8c1d57bfd06aae2f74da97145da1e069da851bffda8492ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial activity</topic><topic>Bacteria</topic><topic>Bacterial Infections</topic><topic>Biofilm eradication</topic><topic>Biofilms</topic><topic>Chitosan - pharmacology</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetic wound healing</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Macrophages</topic><topic>Macrophages polarization</topic><topic>Metal Nanoparticles</topic><topic>Nanogels</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - pharmacology</topic><topic>Silver - pharmacology</topic><topic>Vascular Endothelial Growth Factor A - pharmacology</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Qinqin</creatorcontrib><creatorcontrib>Yang, Zheng</creatorcontrib><creatorcontrib>Tao, Xinyue</creatorcontrib><creatorcontrib>Ma, Chenyu</creatorcontrib><creatorcontrib>Cao, Peiyao</creatorcontrib><creatorcontrib>Wei, Ping</creatorcontrib><creatorcontrib>Jiang, Chenxiao</creatorcontrib><creatorcontrib>Ren, Hao</creatorcontrib><creatorcontrib>Li, Xueming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Qinqin</au><au>Yang, Zheng</au><au>Tao, Xinyue</au><au>Ma, Chenyu</au><au>Cao, Peiyao</au><au>Wei, Ping</au><au>Jiang, Chenxiao</au><au>Ren, Hao</au><au>Li, Xueming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sprayable chitosan nanogel with nitric oxide to accelerate diabetic wound healing through bacteria inhibition, biofilm eradication and macrophage polarization</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-01</date><risdate>2024</risdate><volume>254</volume><issue>Pt 1</issue><spage>127806</spage><epage>127806</epage><pages>127806-127806</pages><artnum>127806</artnum><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Bacterial infection and chronic inflammation are two major risks in diabetic wound healing, which increase patient mortality. In this study, a multifunctional sprayable nanogel (Ag-G@CS) based on chitosan has been developed to synergistically inhibit bacterial infection, eradicate biofilm, and relieve inflammation of diabetic wounds. The nanogel is successfully crafted by encapsulating with a nitric oxide (NO) donor and performing in-situ reduction of silver nanoparticles (Ag). The released NO enhances the antibacterial efficacy of Ag, nearly achieving complete eradication of biofilms in vitro. Upon application on both normal or diabetic chronic wounds, the combination effects of released NO and Ag offer a notable antibacterial effect. Furthermore, after bacteria inhibition and biofilm eradication, the NO released by the nanogel orchestrates a transformation of M1 macrophages into M2 macrophages, significantly reducing tumor necrosis factor α (TNF-α) release and relieving inflammation. Remarkably, the released NO also promotes M2a to M2c macrophages, thereby facilitating tissue remodeling in chronic wounds. More importantly, it upregulates the expression of vascular endothelial growth factor (VEGF), further accelerating the wound healing process. Collectively, the formed sprayable nanogel exhibits excellent inhibition of bacterial infections and biofilms, and promotes chronic wound healing via inflammation resolution, which has excellent potential for clinical use in the future.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>37918593</pmid><doi>10.1016/j.ijbiomac.2023.127806</doi><tpages>1</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0141-8130 |
ispartof | International journal of biological macromolecules, 2024-01, Vol.254 (Pt 1), p.127806-127806, Article 127806 |
issn | 0141-8130 1879-0003 |
language | eng |
recordid | cdi_proquest_miscellaneous_2886331002 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Animals Anti-Bacterial Agents - pharmacology Antibacterial activity Bacteria Bacterial Infections Biofilm eradication Biofilms Chitosan - pharmacology Diabetes Mellitus, Experimental - metabolism Diabetic wound healing Humans Inflammation Macrophages Macrophages polarization Metal Nanoparticles Nanogels Nitric oxide Nitric Oxide - pharmacology Silver - pharmacology Vascular Endothelial Growth Factor A - pharmacology Wound Healing |
title | Sprayable chitosan nanogel with nitric oxide to accelerate diabetic wound healing through bacteria inhibition, biofilm eradication and macrophage polarization |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T03%3A56%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sprayable%20chitosan%20nanogel%20with%20nitric%20oxide%20to%20accelerate%20diabetic%20wound%20healing%20through%20bacteria%20inhibition,%20biofilm%20eradication%20and%20macrophage%20polarization&rft.jtitle=International%20journal%20of%20biological%20macromolecules&rft.au=Huang,%20Qinqin&rft.date=2024-01&rft.volume=254&rft.issue=Pt%201&rft.spage=127806&rft.epage=127806&rft.pages=127806-127806&rft.artnum=127806&rft.issn=0141-8130&rft.eissn=1879-0003&rft_id=info:doi/10.1016/j.ijbiomac.2023.127806&rft_dat=%3Cproquest_cross%3E2886331002%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2886331002&rft_id=info:pmid/37918593&rft_els_id=S0141813023047050&rfr_iscdi=true |